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The map label for this gene is paaF [C]

Identifier: 158316309

GI number: 158316309

Start: 5412152

End: 5412904

Strand: Reverse

Name: paaF [C]

Synonym: Franean1_4536

Alternate gene names: 158316309

Gene position: 5412904-5412152 (Counterclockwise)

Preceding gene: 158316310

Following gene: 158316308

Centisome position: 60.26

GC content: 70.12

Gene sequence:

>753_bases
ATGTCACTGGGCCTGGTCACTGTCGATCTTCAGGGAAGCGTGGCGGTGGTGGAGATGCACCGACCGCCGAACAACTTCTT
CGACGAGGCCAGCCTCCGAGAGCTGGCTCAGGCCCTGCTGCGCGTCGACGCCATGCCCGAGGCGCGCTGTGTGGTGCTGT
GCGCGGAGGGGCGGCACTTCTGCGCGGGCGCTGATCTGCGCGACGTCGACGCGGACGGACTGCGTCGGGTCTACCGCGAG
GCGTTCGCCATCTTCACCGCACGCAAGCCCGTTATCGCCGCCGTACAGGGCGCCGCGATCGGTGGTGGTCTCGGGCTGGC
CCTGGCCGCCGACTTCCGGGTTGCCGCCGAAAACGCGCGGTTCAGCGCGAACTTCGCCCGGCTGGGCTTCCACCAGGGCT
TCGGTCTCTCGGTCACCCTGCCGGCGGCTGTCGGGTCACAGAAGGCGCGCGATCTGCTGTACACAGGTCGCTCGGTTAAC
GGTGCTGAGGCGCTCGCGATGGGGCTGTGTGACCGGGTCACGGCGGCCGACCCGCGGGAGGAGGCGCTGGCCTGGGCCCA
TGACGTCGCCCTGTCGGCGCCGCTGTCCCTGCTGTCGATCAGGTCGACCCTGCGGCGTGAGCTCGTGGCGCGGGTGTCGG
CGGCTCTCGACCAGGAGGCTGCCGCGCAGGCGGTCCTGCTCGGGACCGACGACTTCCGGGAGGGAGTCCAGGCTTCAATC
GGCAAGCGGGCACCATCCTTCACCGGAGCCTGA

Upstream 100 bases:

>100_bases
GTCAACGTGGGGATGTGGGGCGACGGCGGCGGCCGGCCGCACCTCACGGGACGCGACGGTCGTCGTCCCGGGCGGCGCCG
AACCAGAGGAGGTACGCGGG

Downstream 100 bases:

>100_bases
GCAGGTGTTGCGCCGAGGTCGGGGTTAAATATGGCCATGACCGATCATCCGTCCCCGAAGCTCGACGGTGCTCCGCAGAA
CCACCGGACGATCGACCGGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSLGLVTVDLQGSVAVVEMHRPPNNFFDEASLRELAQALLRVDAMPEARCVVLCAEGRHFCAGADLRDVDADGLRRVYRE
AFAIFTARKPVIAAVQGAAIGGGLGLALAADFRVAAENARFSANFARLGFHQGFGLSVTLPAAVGSQKARDLLYTGRSVN
GAEALAMGLCDRVTAADPREEALAWAHDVALSAPLSLLSIRSTLRRELVARVSAALDQEAAAQAVLLGTDDFREGVQASI
GKRAPSFTGA

Sequences:

>Translated_250_residues
MSLGLVTVDLQGSVAVVEMHRPPNNFFDEASLRELAQALLRVDAMPEARCVVLCAEGRHFCAGADLRDVDADGLRRVYRE
AFAIFTARKPVIAAVQGAAIGGGLGLALAADFRVAAENARFSANFARLGFHQGFGLSVTLPAAVGSQKARDLLYTGRSVN
GAEALAMGLCDRVTAADPREEALAWAHDVALSAPLSLLSIRSTLRRELVARVSAALDQEAAAQAVLLGTDDFREGVQASI
GKRAPSFTGA
>Mature_249_residues
SLGLVTVDLQGSVAVVEMHRPPNNFFDEASLRELAQALLRVDAMPEARCVVLCAEGRHFCAGADLRDVDADGLRRVYREA
FAIFTARKPVIAAVQGAAIGGGLGLALAADFRVAAENARFSANFARLGFHQGFGLSVTLPAAVGSQKARDLLYTGRSVNG
AEALAMGLCDRVTAADPREEALAWAHDVALSAPLSLLSIRSTLRRELVARVSAALDQEAAAQAVLLGTDDFREGVQASIG
KRAPSFTGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=29.0697674418605, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=27.0491803278689, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI31542718, Length=237, Percent_Identity=28.6919831223629, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI20127408, Length=226, Percent_Identity=26.9911504424779, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI62530384, Length=251, Percent_Identity=25.8964143426295, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=32.6693227091633, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=30.7392996108949, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=29.6, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=31.9066147859922, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790281, Length=163, Percent_Identity=30.0613496932515, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=272, Percent_Identity=30.5147058823529, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=31.1224489795918, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=30.4878048780488, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=225, Percent_Identity=29.7777777777778, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=28.5123966942149, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=31.8435754189944, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=179, Percent_Identity=29.608938547486, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=218, Percent_Identity=27.5229357798165, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=31.8435754189944, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=179, Percent_Identity=31.8435754189944, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=26.1780104712042, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=31.3492063492063, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=28.4, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=27.1604938271605, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=27.1604938271605, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357171, Length=178, Percent_Identity=28.6516853932584, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26316; Mature: 26185

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLGLVTVDLQGSVAVVEMHRPPNNFFDEASLRELAQALLRVDAMPEARCVVLCAEGRHF
CCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEE
CAGADLRDVDADGLRRVYREAFAIFTARKPVIAAVQGAAIGGGLGLALAADFRVAAENAR
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCC
FSANFARLGFHQGFGLSVTLPAAVGSQKARDLLYTGRSVNGAEALAMGLCDRVTAADPRE
CCCHHHHHCCCCCCCEEEEECHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
EALAWAHDVALSAPLSLLSIRSTLRRELVARVSAALDQEAAAQAVLLGTDDFREGVQASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHH
GKRAPSFTGA
HCCCCCCCCC
>Mature Secondary Structure 
SLGLVTVDLQGSVAVVEMHRPPNNFFDEASLRELAQALLRVDAMPEARCVVLCAEGRHF
CCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEE
CAGADLRDVDADGLRRVYREAFAIFTARKPVIAAVQGAAIGGGLGLALAADFRVAAENAR
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHEECCCCC
FSANFARLGFHQGFGLSVTLPAAVGSQKARDLLYTGRSVNGAEALAMGLCDRVTAADPRE
CCCHHHHHCCCCCCCEEEEECHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
EALAWAHDVALSAPLSLLSIRSTLRRELVARVSAALDQEAAAQAVLLGTDDFREGVQASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHH
GKRAPSFTGA
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA