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The map label for this gene is fadE25 [H]

Identifier: 158316085

GI number: 158316085

Start: 5141153

End: 5142241

Strand: Reverse

Name: fadE25 [H]

Synonym: Franean1_4303

Alternate gene names: 158316085

Gene position: 5142241-5141153 (Counterclockwise)

Preceding gene: 158316086

Following gene: 158316083

Centisome position: 57.25

GC content: 70.71

Gene sequence:

>1089_bases
TTGGACTTTCGTGACTCGCCGGAGGAGGCCGCCTTCCGCACGCGGCTGCAGGACTGGCTCGCCGAGAACGCGAAGAAGTT
CCCGACGTCGGGTGACGAGTACTGGGCCAAGGCGGCGCAGTGGCACCAGGCCCTGTACGCGGGCGGGTTCTTCGGGACCT
CCTGGCCGAAGGCGTACGGCGGCCAGGAGCTGCCGCCCGTCTACGACGTGATCGTCGACGAGGAGCTGGCCCGCGCCGGC
GCGCCGGCGCGGCCGAGCCTGGGCTACCTGGTCATCGGCCTGGGCCGGCACGGCAGCGAGGAGCTGCGGCAGCGATTCCT
GCCGGGCATGATCAACGGCGCTGAGCGCTGGTGCCAGGGCTTCAGCGAGCCCGGCGCCGGCTCCGACCTGGCCTCCCTGA
CCACGACGGCCACCCTGGAGGGCGACCACTACGTCCTCAACGGCCACAAGATCTGGACGAGCTACTCCGATGACGCCGAC
TGGTGCCTCGTGCTCGCCCGCACCGACCCGGACGCCCCACGGCACAAGGGCATCTCCGGATTCATCGTGAACATGCACCA
GCCGGGCATCGAGCAGCGCCCGCTGCCGATGATCAGCGGGGTGACCAAGGAGTTCGGCCAGGTCCTGTTCGACGGCGCGA
CGGTGCCGGCCGACCAGATAGTCGGCGCGCCCGGCGAGGGCTGGAAGCTGGCCATGACGGTCGTCAGCCACGAGCGGGAG
CCCTCGACGCTGGGCTTCACCGCGCGGTACGGAAAGACCGTGCGCAACCTGGCGGCCCGCCTGGACGGGGAGCCGTCGGA
GTCGCTGCGGTGGGCCGCCGTGCAGACCGAGATGCTGCGCCTGCACGTGCGCCGGCGGCTGTCCGAGCAGCTCGACGGGC
TCAGCCACGGCCCGGACGGCTCGCTGGACAAGCTGTTGATGACCTGGGTCGAGCAGTCGGTGGGGCACGCCTCGCTGGAC
GCGGGCGGCACTCGCGACGCCGAGCTGCTGGGCACCTACCTCTACAGCCGCTCGCAGAGCGTCATGGGCGGTACGTCGCA
GATCCAGAAGAACATCATCGCGACCCGCATCATCGGCCTGGAGGGCTGA

Upstream 100 bases:

>100_bases
GGCGGCATCGGCAACACCTGGGAGTGCCTCGCCCACGTCTTCCTGCGCCGCGCGCTGACGTCCGGCGCGCTGTTCCCCGT
CTCTCTGGAGGAGATCGACC

Downstream 100 bases:

>100_bases
CCCGGAACGGGCGCCACCGCCGGTCGGCGGTGGCGCGGGGAGCCACTCCCCGCGCCACCGCCGAGGCGGTGCGGGGACTA
CTTCGGCGGGAAGCGCAGCG

Product: acyl-CoA dehydrogenase domain-containing protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MDFRDSPEEAAFRTRLQDWLAENAKKFPTSGDEYWAKAAQWHQALYAGGFFGTSWPKAYGGQELPPVYDVIVDEELARAG
APARPSLGYLVIGLGRHGSEELRQRFLPGMINGAERWCQGFSEPGAGSDLASLTTTATLEGDHYVLNGHKIWTSYSDDAD
WCLVLARTDPDAPRHKGISGFIVNMHQPGIEQRPLPMISGVTKEFGQVLFDGATVPADQIVGAPGEGWKLAMTVVSHERE
PSTLGFTARYGKTVRNLAARLDGEPSESLRWAAVQTEMLRLHVRRRLSEQLDGLSHGPDGSLDKLLMTWVEQSVGHASLD
AGGTRDAELLGTYLYSRSQSVMGGTSQIQKNIIATRIIGLEG

Sequences:

>Translated_362_residues
MDFRDSPEEAAFRTRLQDWLAENAKKFPTSGDEYWAKAAQWHQALYAGGFFGTSWPKAYGGQELPPVYDVIVDEELARAG
APARPSLGYLVIGLGRHGSEELRQRFLPGMINGAERWCQGFSEPGAGSDLASLTTTATLEGDHYVLNGHKIWTSYSDDAD
WCLVLARTDPDAPRHKGISGFIVNMHQPGIEQRPLPMISGVTKEFGQVLFDGATVPADQIVGAPGEGWKLAMTVVSHERE
PSTLGFTARYGKTVRNLAARLDGEPSESLRWAAVQTEMLRLHVRRRLSEQLDGLSHGPDGSLDKLLMTWVEQSVGHASLD
AGGTRDAELLGTYLYSRSQSVMGGTSQIQKNIIATRIIGLEG
>Mature_362_residues
MDFRDSPEEAAFRTRLQDWLAENAKKFPTSGDEYWAKAAQWHQALYAGGFFGTSWPKAYGGQELPPVYDVIVDEELARAG
APARPSLGYLVIGLGRHGSEELRQRFLPGMINGAERWCQGFSEPGAGSDLASLTTTATLEGDHYVLNGHKIWTSYSDDAD
WCLVLARTDPDAPRHKGISGFIVNMHQPGIEQRPLPMISGVTKEFGQVLFDGATVPADQIVGAPGEGWKLAMTVVSHERE
PSTLGFTARYGKTVRNLAARLDGEPSESLRWAAVQTEMLRLHVRRRLSEQLDGLSHGPDGSLDKLLMTWVEQSVGHASLD
AGGTRDAELLGTYLYSRSQSVMGGTSQIQKNIIATRIIGLEG

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=230, Percent_Identity=33.0434782608696, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI226958414, Length=197, Percent_Identity=33.502538071066, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI4557231, Length=335, Percent_Identity=26.2686567164179, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI187960098, Length=335, Percent_Identity=26.2686567164179, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4501859, Length=231, Percent_Identity=31.6017316017316, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4557233, Length=196, Percent_Identity=33.1632653061224, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4501857, Length=387, Percent_Identity=25.8397932816537, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI21361497, Length=346, Percent_Identity=25.1445086705202, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI48976061, Length=306, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI211971008, Length=306, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4557235, Length=237, Percent_Identity=30.379746835443, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI76496475, Length=237, Percent_Identity=29.957805907173, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI7656849, Length=194, Percent_Identity=30.4123711340206, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI7669494, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI4503943, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786223, Length=245, Percent_Identity=27.7551020408163, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081958, Length=243, Percent_Identity=27.1604938271605, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17570075, Length=394, Percent_Identity=27.4111675126904, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538396, Length=246, Percent_Identity=33.739837398374, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17534899, Length=227, Percent_Identity=34.3612334801762, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17569725, Length=386, Percent_Identity=26.6839378238342, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17506239, Length=389, Percent_Identity=25.9640102827763, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17508101, Length=362, Percent_Identity=27.0718232044199, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551932, Length=236, Percent_Identity=27.5423728813559, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505929, Length=198, Percent_Identity=29.2929292929293, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32563615, Length=197, Percent_Identity=31.9796954314721, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71985184, Length=163, Percent_Identity=33.7423312883436, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71985192, Length=163, Percent_Identity=33.7423312883436, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982178, Length=243, Percent_Identity=28.8065843621399, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71990804, Length=206, Percent_Identity=30.0970873786408, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533517, Length=191, Percent_Identity=28.7958115183246, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24666513, Length=345, Percent_Identity=28.4057971014493, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24646207, Length=338, Percent_Identity=28.4023668639053, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356377, Length=196, Percent_Identity=32.1428571428571, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355753, Length=242, Percent_Identity=30.5785123966942, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24660351, Length=260, Percent_Identity=29.6153846153846, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281363737, Length=211, Percent_Identity=28.9099526066351, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 39610; Mature: 39610

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRDSPEEAAFRTRLQDWLAENAKKFPTSGDEYWAKAAQWHQALYAGGFFGTSWPKAYG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCC
GQELPPVYDVIVDEELARAGAPARPSLGYLVIGLGRHGSEELRQRFLPGMINGAERWCQG
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHC
FSEPGAGSDLASLTTTATLEGDHYVLNGHKIWTSYSDDADWCLVLARTDPDAPRHKGISG
CCCCCCCCCHHHHHEEEEECCCEEEECCEEEEECCCCCCCEEEEEEECCCCCHHHCCCCE
FIVNMHQPGIEQRPLPMISGVTKEFGQVLFDGATVPADQIVGAPGEGWKLAMTVVSHERE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEHHCCCC
PSTLGFTARYGKTVRNLAARLDGEPSESLRWAAVQTEMLRLHVRRRLSEQLDGLSHGPDG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SLDKLLMTWVEQSVGHASLDAGGTRDAELLGTYLYSRSQSVMGGTSQIQKNIIATRIIGL
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHEECC
EG
CC
>Mature Secondary Structure
MDFRDSPEEAAFRTRLQDWLAENAKKFPTSGDEYWAKAAQWHQALYAGGFFGTSWPKAYG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCC
GQELPPVYDVIVDEELARAGAPARPSLGYLVIGLGRHGSEELRQRFLPGMINGAERWCQG
CCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHC
FSEPGAGSDLASLTTTATLEGDHYVLNGHKIWTSYSDDADWCLVLARTDPDAPRHKGISG
CCCCCCCCCHHHHHEEEEECCCEEEECCEEEEECCCCCCCEEEEEEECCCCCHHHCCCCE
FIVNMHQPGIEQRPLPMISGVTKEFGQVLFDGATVPADQIVGAPGEGWKLAMTVVSHERE
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEEHHCCCC
PSTLGFTARYGKTVRNLAARLDGEPSESLRWAAVQTEMLRLHVRRRLSEQLDGLSHGPDG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SLDKLLMTWVEQSVGHASLDAGGTRDAELLGTYLYSRSQSVMGGTSQIQKNIIATRIIGL
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHEECC
EG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]