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The map label for this gene is gsiB [H]

Identifier: 158316004

GI number: 158316004

Start: 5028475

End: 5030040

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_4222

Alternate gene names: 158316004

Gene position: 5030040-5028475 (Counterclockwise)

Preceding gene: 158316005

Following gene: 158316003

Centisome position: 56.0

GC content: 66.6

Gene sequence:

>1566_bases
ATGATGTTCAGTAGGTCTCGATTAATCGTTACGGCCGTTGTGTGCGGTGCGGTTCTGGCTCTGGGGGCCTGTGGTGGTGC
TGATCCCGCGTCGCCGACGGGCGGTGCCACCGGCGAGCCGGTCGCGGGTGGTCATGGCCGCATCCTGATGCTCAGCGACC
CCCGTAGCCTGGACCCGGCGACGCTCGGCAACGCCTACGCGACCACCGGCGCCCTCGGTAACGCCCTGTACGGCACCTTG
ATGACGACCGACGATGCCGGTGAGATCCAGTACACGATGGCCGAGTCGTTCACCACCACCGACGGCGGCGCGACCTTCAC
CCTGAAACTGCGCCCCGGCCTGACGTTCTCCGACGGCACCCCGCTGGACGCCGAAGCGGTGAAGTTCGACTGGGACCGCC
TCAAGGATCCGGCCACCCGCGCGACCAACCTGTCCGAAGCATCGATGATCTCCTCGACCGAGGTCGTCGACAGCACCACA
CTGAAGATCACCATGGTGGCGCCCGCACCGAAGTACGCCCACTCGGTCATCACCTCCACCCTGAACTGGATCGCCTCACC
CGCGGCTCTGCAAAAGGGCGCGCAGGCCTTCGACGCGGCCCCGGTCGGTGCCGGGCCGTTCACCCTGACGAGCTGGACCC
GCCAGGCAGCCATCGAACTGGCCAGGAACCCCCGCTACTGGGACGCACCCAGGCCCTACCTCGACCGTCTCACCCTGCGC
ACCACCTCCGACACCGGCCAGCGCTTCAACACGGTGCTCACCGGTGGCGCGGACGTGGCCATCGAGTCGAACCCGGTCAA
CATCGAAAAGGCCACCGACGCCGGCCTGCCCACCACCGTCATGGCCCTCAGCGGTGGCACCTTCATCGCGCTGAACACCC
GCCGGGCACCCTTCGACGACGTCCGCGCCCGCCAGGCCGTCGCCGCCGCCCTCGACATGGACGCGCTGAACCTCGCCGTC
TACAACGGCAAGGGCGAACCTGTCGACACCCTGTTCAGCGACACCTCGCCGTTCCACTCGGACACACCACTGCGCACGAC
GGACAAGGCGACCGCCCAACGGCTCTTCGACGAACTCGCCGCCGAAGGCAAGCCGGTGACCTTCACCTACTCCAGCGCTC
CCACCACCGAGAACAGAAACACAGCCGAGAACATTCAGGCCCAGCTCGGCGCTTTCAAAAACGTCAAAGTCAACGTCAAG
GTCATCGAAGTGACCGAACTCGCCGCGCTACGCACGACCCACGACTTCGACGCGGCCACCTCGTCGGCGTTCTTCCAGGA
CCCCGAGCCACGCCTGTGGACGGCCTTCGCCGCCAGTTCGGCCGCGAACCTGTCCGGGATCAACGACCAGGAACTCAACG
ACGCCCTCCTCGCCGGCCGGACCGGTACGTCGGAACAGGAACGCGCAGCCGCCTACAAGACGGTGCAGCAGCGACTCACC
GAGCTGTCCCCGGTGGTCTTCCTCACTCGAGCCGAACCCAGCGCCATCGCGGGAAAGAACGTGGGCGGCCTCATCCAGTA
CGGACTCGGATCTCTGCTGCCCGACCAGATCTGGATCCAGAAGTAG

Upstream 100 bases:

>100_bases
CGCTGCTTTACAGCGGAAGTCGGCGCCGTGGCGCAGGTGCGGAAACCGCGCAGTAGAAGACCCGAAAACGTTCCTGATAT
CAATCGCGAGGGAAAAGTTC

Downstream 100 bases:

>100_bases
CGGTCGTTCCCGATCCGGGCACCGACCCGACGACAGATGCGCTCCGACCCGTCGGAGATCACCCAGGGGGTACTTGTGGA
CACTCCGCCGATTTCCCTCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MMFSRSRLIVTAVVCGAVLALGACGGADPASPTGGATGEPVAGGHGRILMLSDPRSLDPATLGNAYATTGALGNALYGTL
MTTDDAGEIQYTMAESFTTTDGGATFTLKLRPGLTFSDGTPLDAEAVKFDWDRLKDPATRATNLSEASMISSTEVVDSTT
LKITMVAPAPKYAHSVITSTLNWIASPAALQKGAQAFDAAPVGAGPFTLTSWTRQAAIELARNPRYWDAPRPYLDRLTLR
TTSDTGQRFNTVLTGGADVAIESNPVNIEKATDAGLPTTVMALSGGTFIALNTRRAPFDDVRARQAVAAALDMDALNLAV
YNGKGEPVDTLFSDTSPFHSDTPLRTTDKATAQRLFDELAAEGKPVTFTYSSAPTTENRNTAENIQAQLGAFKNVKVNVK
VIEVTELAALRTTHDFDAATSSAFFQDPEPRLWTAFAASSAANLSGINDQELNDALLAGRTGTSEQERAAAYKTVQQRLT
ELSPVVFLTRAEPSAIAGKNVGGLIQYGLGSLLPDQIWIQK

Sequences:

>Translated_521_residues
MMFSRSRLIVTAVVCGAVLALGACGGADPASPTGGATGEPVAGGHGRILMLSDPRSLDPATLGNAYATTGALGNALYGTL
MTTDDAGEIQYTMAESFTTTDGGATFTLKLRPGLTFSDGTPLDAEAVKFDWDRLKDPATRATNLSEASMISSTEVVDSTT
LKITMVAPAPKYAHSVITSTLNWIASPAALQKGAQAFDAAPVGAGPFTLTSWTRQAAIELARNPRYWDAPRPYLDRLTLR
TTSDTGQRFNTVLTGGADVAIESNPVNIEKATDAGLPTTVMALSGGTFIALNTRRAPFDDVRARQAVAAALDMDALNLAV
YNGKGEPVDTLFSDTSPFHSDTPLRTTDKATAQRLFDELAAEGKPVTFTYSSAPTTENRNTAENIQAQLGAFKNVKVNVK
VIEVTELAALRTTHDFDAATSSAFFQDPEPRLWTAFAASSAANLSGINDQELNDALLAGRTGTSEQERAAAYKTVQQRLT
ELSPVVFLTRAEPSAIAGKNVGGLIQYGLGSLLPDQIWIQK
>Mature_521_residues
MMFSRSRLIVTAVVCGAVLALGACGGADPASPTGGATGEPVAGGHGRILMLSDPRSLDPATLGNAYATTGALGNALYGTL
MTTDDAGEIQYTMAESFTTTDGGATFTLKLRPGLTFSDGTPLDAEAVKFDWDRLKDPATRATNLSEASMISSTEVVDSTT
LKITMVAPAPKYAHSVITSTLNWIASPAALQKGAQAFDAAPVGAGPFTLTSWTRQAAIELARNPRYWDAPRPYLDRLTLR
TTSDTGQRFNTVLTGGADVAIESNPVNIEKATDAGLPTTVMALSGGTFIALNTRRAPFDDVRARQAVAAALDMDALNLAV
YNGKGEPVDTLFSDTSPFHSDTPLRTTDKATAQRLFDELAAEGKPVTFTYSSAPTTENRNTAENIQAQLGAFKNVKVNVK
VIEVTELAALRTTHDFDAATSSAFFQDPEPRLWTAFAASSAANLSGINDQELNDALLAGRTGTSEQERAAAYKTVQQRLT
ELSPVVFLTRAEPSAIAGKNVGGLIQYGLGSLLPDQIWIQK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=462, Percent_Identity=25.974025974026, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789966, Length=457, Percent_Identity=24.0700218818381, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=23.2645403377111, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789397, Length=534, Percent_Identity=23.2209737827715, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787762, Length=495, Percent_Identity=23.4343434343434, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081878, Length=519, Percent_Identity=23.3140655105973, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789887, Length=269, Percent_Identity=24.907063197026, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787551, Length=477, Percent_Identity=22.2222222222222, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082063, Length=296, Percent_Identity=22.6351351351351, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55023; Mature: 55023

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFSRSRLIVTAVVCGAVLALGACGGADPASPTGGATGEPVAGGHGRILMLSDPRSLDPA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
TLGNAYATTGALGNALYGTLMTTDDAGEIQYTMAESFTTTDGGATFTLKLRPGLTFSDGT
HCCCCHHHHHHHHHHHEEEEEECCCCCCEEEEEECCEEECCCCEEEEEEECCCEEECCCC
PLDAEAVKFDWDRLKDPATRATNLSEASMISSTEVVDSTTLKITMVAPAPKYAHSVITST
CCCHHHEECCHHHCCCCHHHHCCCCHHHHHCCHHEECCCEEEEEEECCCCHHHHHHHHHH
LNWIASPAALQKGAQAFDAAPVGAGPFTLTSWTRQAAIELARNPRYWDAPRPYLDRLTLR
HHHHCCCHHHHHCHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEE
TTSDTGQRFNTVLTGGADVAIESNPVNIEKATDAGLPTTVMALSGGTFIALNTRRAPFDD
ECCCCCCCEEEEEECCCEEEEECCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCHHH
VRARQAVAAALDMDALNLAVYNGKGEPVDTLFSDTSPFHSDTPLRTTDKATAQRLFDELA
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEGKPVTFTYSSAPTTENRNTAENIQAQLGAFKNVKVNVKVIEVTELAALRTTHDFDAAT
HCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHCCCCCCCCC
SSAFFQDPEPRLWTAFAASSAANLSGINDQELNDALLAGRTGTSEQERAAAYKTVQQRLT
CCCCCCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELSPVVFLTRAEPSAIAGKNVGGLIQYGLGSLLPDQIWIQK
HCCCEEEEEECCCCHHCCCCCCHHHHHHHHHCCCCHHEECC
>Mature Secondary Structure
MMFSRSRLIVTAVVCGAVLALGACGGADPASPTGGATGEPVAGGHGRILMLSDPRSLDPA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCH
TLGNAYATTGALGNALYGTLMTTDDAGEIQYTMAESFTTTDGGATFTLKLRPGLTFSDGT
HCCCCHHHHHHHHHHHEEEEEECCCCCCEEEEEECCEEECCCCEEEEEEECCCEEECCCC
PLDAEAVKFDWDRLKDPATRATNLSEASMISSTEVVDSTTLKITMVAPAPKYAHSVITST
CCCHHHEECCHHHCCCCHHHHCCCCHHHHHCCHHEECCCEEEEEEECCCCHHHHHHHHHH
LNWIASPAALQKGAQAFDAAPVGAGPFTLTSWTRQAAIELARNPRYWDAPRPYLDRLTLR
HHHHCCCHHHHHCHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEE
TTSDTGQRFNTVLTGGADVAIESNPVNIEKATDAGLPTTVMALSGGTFIALNTRRAPFDD
ECCCCCCCEEEEEECCCEEEEECCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCHHH
VRARQAVAAALDMDALNLAVYNGKGEPVDTLFSDTSPFHSDTPLRTTDKATAQRLFDELA
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEGKPVTFTYSSAPTTENRNTAENIQAQLGAFKNVKVNVKVIEVTELAALRTTHDFDAAT
HCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHCCCCCCCCC
SSAFFQDPEPRLWTAFAASSAANLSGINDQELNDALLAGRTGTSEQERAAAYKTVQQRLT
CCCCCCCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELSPVVFLTRAEPSAIAGKNVGGLIQYGLGSLLPDQIWIQK
HCCCEEEEEECCCCHHCCCCCCHHHHHHHHHCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA