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The map label for this gene is gsiB [H]

Identifier: 158315996

GI number: 158315996

Start: 5019629

End: 5021074

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_4214

Alternate gene names: 158315996

Gene position: 5021074-5019629 (Counterclockwise)

Preceding gene: 158315997

Following gene: 158315994

Centisome position: 55.9

GC content: 67.63

Gene sequence:

>1446_bases
GTGGCGGGCGGCCACGGGCGAATCCTGACACTGAGCGATCTCCGCAGCCTGGACCCGGGGACGATCGGCAACGCCTATGC
GAGCACAGGTGTCGTGGGTAACGCCCTGTACGGAACGTTGATGACCAACGACGAGGCGGGCGAGATCCGCTACCAGATGG
CCGAGTCCTTCACCACGACGGACAACGGCCGCACCTTCACCCTGAAGCTGCGGCCCGGGCTGGTCTTCTCCGACGGCACT
CCGCTCAATGCCGAAGCGGTGAAGTTCAACTGGGATCGGGTCAAGGATCCGGCCACCGGCTCTCCCCACCGCTCGGAGGC
GTCGATGATCGCCTCGTCCGAGGTGGTGGACGACGTCACGCTGACCGCCACGATGGTCACCCAGGTGCCCAAGTATGCCC
AGTCCGTCGTCACCTCGTCACTGAACTGGATCGCCTCGCCCGCGGCGCTGCGGAAGGGCACCGCGGCCTTCGACGCCGCC
CCGATCGGCGCCGGCCCGTTCACGCTGAAGAGCTGGACCCGCCAGGCCGCCATGGAGCTGGTCAAGAACCCCCGCTACTG
GGACGCCCCCAAGCCGTACCTGGACACCCTCACCTTCCAGGCCGCGCTCGACTCCAACCAGCGTTACAACACCCTGCAGA
CCGGGGGCGCGGACGTCGCCGTCGAGTCGAATCCCGTCAACTTCGACAAGGCCGACAAGGCCGGCCTGCCGAACACCGTG
CTGGAGCTCAGCGGCGGCCTCTTCATGGCGATGAACACCCGCAGGGCGCCGTTCGACGACGTCCGCGCACGGCAGGCCGT
CGCCGGGGCACTTGACCTGGACGCGCTGAACCTCGCCGCCTACAACGGAACCGGCACACCCGTCGACACGCTGTTCAGCA
AGGCCTCGCCCTTCTACTCGGACACGCCGCTGCGCAAGGCGGACAAGGCCACCGCCCAGCGACTGTTCGACGAGCTGGCC
GCGGAAGGCAAGCCGGTGTCCTTCGCCGTCTCCACCGCCCCCACCTCGGAGAGTAGGACGACGGCGGAGAACATCCAGGC
CCAGCTCGGCACGTTCAAAAACGTCAAGGTCCAGGTCAAGATTATCGAGGTTGCCGACGTCGTCTCGCTGCGCACGACGC
ATGACTTCGACGCGACCACGTCGTCAGCGTTCTTCCAGGACCCCGAACCACGGCTGTGGACGGCCTTCAGCGGCGACTCG
CCGGCGAACCTGTCCGGGATCGCCGACAAGGCGCTCAACGACGCCCTGCTGGCGGGGCGGACAGGTACATCGGAACAGGA
CCGCGAGAAGGCCTACGACACGGTCCAGCAGCGCCTCACCGAGCTGGCCCCGGTGGTCTTCTTCACGCGAGCGGAGCCGG
GCGCCATCGCCGGCAAGGACGTCGGCGGCCTCGTGCAGTACGGCCTCGGCTCGCTCCTGCCCGAGGAACTCTGGATCCAG
AAGTAG

Upstream 100 bases:

>100_bases
ACTGGCCACGGCTGTTACATGTTGCGCCGCCCTTCTTTTAGGGGCCTGTGGCGGAGGTGGCGATTCCGCCAGCCCGTCCG
CGGGTGCCTCCGGCAAGCCA

Downstream 100 bases:

>100_bases
CCGCCCGCCCCGGACGTCGACCTGACGACTCAGTCCGGGTGCTGAGATCTCGAGATCCTGGGGAAAGATCGAACGGAGAG
ATTTTCGACGGGAAACAGGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAGGHGRILTLSDLRSLDPGTIGNAYASTGVVGNALYGTLMTNDEAGEIRYQMAESFTTTDNGRTFTLKLRPGLVFSDGT
PLNAEAVKFNWDRVKDPATGSPHRSEASMIASSEVVDDVTLTATMVTQVPKYAQSVVTSSLNWIASPAALRKGTAAFDAA
PIGAGPFTLKSWTRQAAMELVKNPRYWDAPKPYLDTLTFQAALDSNQRYNTLQTGGADVAVESNPVNFDKADKAGLPNTV
LELSGGLFMAMNTRRAPFDDVRARQAVAGALDLDALNLAAYNGTGTPVDTLFSKASPFYSDTPLRKADKATAQRLFDELA
AEGKPVSFAVSTAPTSESRTTAENIQAQLGTFKNVKVQVKIIEVADVVSLRTTHDFDATTSSAFFQDPEPRLWTAFSGDS
PANLSGIADKALNDALLAGRTGTSEQDREKAYDTVQQRLTELAPVVFFTRAEPGAIAGKDVGGLVQYGLGSLLPEELWIQ
K

Sequences:

>Translated_481_residues
MAGGHGRILTLSDLRSLDPGTIGNAYASTGVVGNALYGTLMTNDEAGEIRYQMAESFTTTDNGRTFTLKLRPGLVFSDGT
PLNAEAVKFNWDRVKDPATGSPHRSEASMIASSEVVDDVTLTATMVTQVPKYAQSVVTSSLNWIASPAALRKGTAAFDAA
PIGAGPFTLKSWTRQAAMELVKNPRYWDAPKPYLDTLTFQAALDSNQRYNTLQTGGADVAVESNPVNFDKADKAGLPNTV
LELSGGLFMAMNTRRAPFDDVRARQAVAGALDLDALNLAAYNGTGTPVDTLFSKASPFYSDTPLRKADKATAQRLFDELA
AEGKPVSFAVSTAPTSESRTTAENIQAQLGTFKNVKVQVKIIEVADVVSLRTTHDFDATTSSAFFQDPEPRLWTAFSGDS
PANLSGIADKALNDALLAGRTGTSEQDREKAYDTVQQRLTELAPVVFFTRAEPGAIAGKDVGGLVQYGLGSLLPEELWIQ
K
>Mature_480_residues
AGGHGRILTLSDLRSLDPGTIGNAYASTGVVGNALYGTLMTNDEAGEIRYQMAESFTTTDNGRTFTLKLRPGLVFSDGTP
LNAEAVKFNWDRVKDPATGSPHRSEASMIASSEVVDDVTLTATMVTQVPKYAQSVVTSSLNWIASPAALRKGTAAFDAAP
IGAGPFTLKSWTRQAAMELVKNPRYWDAPKPYLDTLTFQAALDSNQRYNTLQTGGADVAVESNPVNFDKADKAGLPNTVL
ELSGGLFMAMNTRRAPFDDVRARQAVAGALDLDALNLAAYNGTGTPVDTLFSKASPFYSDTPLRKADKATAQRLFDELAA
EGKPVSFAVSTAPTSESRTTAENIQAQLGTFKNVKVQVKIIEVADVVSLRTTHDFDATTSSAFFQDPEPRLWTAFSGDSP
ANLSGIADKALNDALLAGRTGTSEQDREKAYDTVQQRLTELAPVVFFTRAEPGAIAGKDVGGLVQYGLGSLLPEELWIQK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=363, Percent_Identity=28.9256198347107, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789966, Length=466, Percent_Identity=26.1802575107296, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1787495, Length=489, Percent_Identity=24.5398773006135, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789397, Length=467, Percent_Identity=24.1970021413276, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789887, Length=471, Percent_Identity=23.7791932059448, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081878, Length=452, Percent_Identity=25.2212389380531, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787762, Length=439, Percent_Identity=22.5512528473804, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082063, Length=314, Percent_Identity=23.8853503184713, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 51438; Mature: 51307

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGHGRILTLSDLRSLDPGTIGNAYASTGVVGNALYGTLMTNDEAGEIRYQMAESFTTT
CCCCCCCEEEHHHHCCCCCCCCCCHHHHCCCCCHHHEEEEEECCCCCHHHHHHHHHCEEC
DNGRTFTLKLRPGLVFSDGTPLNAEAVKFNWDRVKDPATGSPHRSEASMIASSEVVDDVT
CCCEEEEEEECCCEEECCCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LTATMVTQVPKYAQSVVTSSLNWIASPAALRKGTAAFDAAPIGAGPFTLKSWTRQAAMEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VKNPRYWDAPKPYLDTLTFQAALDSNQRYNTLQTGGADVAVESNPVNFDKADKAGLPNTV
HCCCCCCCCCCCHHHHEEEEEHHCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCHHH
LELSGGLFMAMNTRRAPFDDVRARQAVAGALDLDALNLAAYNGTGTPVDTLFSKASPFYS
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCC
DTPLRKADKATAQRLFDELAAEGKPVSFAVSTAPTSESRTTAENIQAQLGTFKNVKVQVK
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
IIEVADVVSLRTTHDFDATTSSAFFQDPEPRLWTAFSGDSPANLSGIADKALNDALLAGR
EEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
TGTSEQDREKAYDTVQQRLTELAPVVFFTRAEPGAIAGKDVGGLVQYGLGSLLPEELWIQ
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHCC
K
C
>Mature Secondary Structure 
AGGHGRILTLSDLRSLDPGTIGNAYASTGVVGNALYGTLMTNDEAGEIRYQMAESFTTT
CCCCCCEEEHHHHCCCCCCCCCCHHHHCCCCCHHHEEEEEECCCCCHHHHHHHHHCEEC
DNGRTFTLKLRPGLVFSDGTPLNAEAVKFNWDRVKDPATGSPHRSEASMIASSEVVDDVT
CCCEEEEEEECCCEEECCCCCCCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LTATMVTQVPKYAQSVVTSSLNWIASPAALRKGTAAFDAAPIGAGPFTLKSWTRQAAMEL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VKNPRYWDAPKPYLDTLTFQAALDSNQRYNTLQTGGADVAVESNPVNFDKADKAGLPNTV
HCCCCCCCCCCCHHHHEEEEEHHCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCHHH
LELSGGLFMAMNTRRAPFDDVRARQAVAGALDLDALNLAAYNGTGTPVDTLFSKASPFYS
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCC
DTPLRKADKATAQRLFDELAAEGKPVSFAVSTAPTSESRTTAENIQAQLGTFKNVKVQVK
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
IIEVADVVSLRTTHDFDATTSSAFFQDPEPRLWTAFSGDSPANLSGIADKALNDALLAGR
EEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
TGTSEQDREKAYDTVQQRLTELAPVVFFTRAEPGAIAGKDVGGLVQYGLGSLLPEELWIQ
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA