Click here to switch to the map view.

The map label for this gene is bmr3 [H]

Identifier: 158315985

GI number: 158315985

Start: 5005969

End: 5007489

Strand: Reverse

Name: bmr3 [H]

Synonym: Franean1_4203

Alternate gene names: 158315985

Gene position: 5007489-5005969 (Counterclockwise)

Preceding gene: 158315987

Following gene: 158315984

Centisome position: 55.75

GC content: 72.06

Gene sequence:

>1521_bases
ATGGCGCGAGCTCGCACGGACCCGGCCGAACCGGGGGCGGGCCCGGCGTCGACGGCACCGATGGGACCGACCGCGCCGCG
CCCGAGCCTGTTGACCGCGGCGCTGGCCGTGGCGGGCATCGTGGCGTCGTTCATGCACACCCTGGTCGTGCCGATCATTC
CGCAGCTCCCGCACCTGTTGGACTCCTCGGCGTCCAACACGACCTGGGTCGTCACGATCACCTTGCTGGCCGGTGCCGTC
GCGACGCCTGTCGCCGGGCGGCTCGGTGACATGTACGGCAAGCGCCGGATCATGCTCGGCAGCGTGGGGCTGCTCGGCGC
CGGCTCGCTGGTCTGCGCGCTGAGCACGTCGTTGCCACAGATGGTTGTCGGCCGCGGGCTGCAGGGGCTGGCCACCGGGC
TGATCCCGCTCGGGATCAGCCTCATGCGCGACGAGCTGCCGCCCGAGCGGCTCGGCTCCGCGCTCGGGCTGATGAGCTCC
TCGCTCGGCATCGGTGGCGCGCTAGGTGTCCCGACCTCGGCGCTGATCGCTCAGAACTTCAGCTGGCACGTGCTCTTCTG
GACGGCCACCGTCCTGAGCGTGCTGGTGCTCGCCATGCTGTGGCGGGTCGTCCCCGAGTCGCCGGTGCGCGACGTCCGCG
GCCGCTTCGACGTCGTCGGGGCGCTCGGGCTCGGCACCGGGATCACCTGCCTGCTGCTGGCGATCTCCAAGGGCAGCACC
TGGGGCTGGACGTCCATCACCACCCTGGGGACGGTGCTCGCCGCGCTCGCGGTGCTGGCCCTGTGGGGCCCGTGGGAGCT
GCGCCACGCCTCCCCGGTGGTCGACCTGCGGGTCAGTGCCCGCCGTCAGGTGCTGATGACCAACCTGACCTCGGTCGTCG
TCGGCTTCGCGATGTACGGGATGGGGCTGGTCATCCCGCAGTTCCTGCAGCTGTCCGCGGGCACCGGCTACGGGCTGGGC
AAGTCGATGGTCGTCGCCGGGCTGTGCTTCGCCCCGTTCGGGGTGGTCATGATGCTCGCCTCGCCGCTCACCGCCCGGGT
GTCGGCGATGTGGGGGTCGAAGACGACGCTGGTGCTCGGTTCCGCGATCATCGGGGTCAGCTACCTGATCGGCCTGTTCG
TGATGCACTCGGTCTGGCAGGTCGTGCTCCTCGCGGTGATCGGCGGGATCGGGGTGGCGCTGGCCTACGCATCCATGCCG
TCGCTGATCATGGCCGCGGTGCCGGCGACCGAGACCGCGGCGGCCAACGGTCTGAACACCCTGATGCGCTCGCTGGGCAC
CTCCTCGTCGGCTGCCATCGTCGGGGTGATGCTGGCCAACATGACCATCACGTTCGGCGGCCGGGAGGTTCCGTCGCTGG
CGGGCTTCCACGCGGTGTTCGCGCTCGGCGCGGGCGCGGCGGCGGCCGCCGTGCTGATGGGCCTGTTCATCCCCGGCCGG
GGGGCGCGGGAGGTGCCCGTCCCGGCTGTCGCGGCCCCGCGTGAGACGGGCCGACCGCGCGCGCAGCTCCAGCAGAGCTG
A

Upstream 100 bases:

>100_bases
CCTGAGCTGGCGTTCGGCCTGGCAGTCTGTCCCGGCGTCGCCGCCGCCGCGCACCACCTTCGTCGAGCCCGCACCACCTT
CGTCGAATCGGAGTACCGCC

Downstream 100 bases:

>100_bases
CAACCTCCGCTACAGCTGACAGCCGCCGGCCGGCCTCACGATCCGGACCCGGATCGTGAGGCCGTGTGCGGACGTCCGAG
CTGATCTTGGGGCGGCGGCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MARARTDPAEPGAGPASTAPMGPTAPRPSLLTAALAVAGIVASFMHTLVVPIIPQLPHLLDSSASNTTWVVTITLLAGAV
ATPVAGRLGDMYGKRRIMLGSVGLLGAGSLVCALSTSLPQMVVGRGLQGLATGLIPLGISLMRDELPPERLGSALGLMSS
SLGIGGALGVPTSALIAQNFSWHVLFWTATVLSVLVLAMLWRVVPESPVRDVRGRFDVVGALGLGTGITCLLLAISKGST
WGWTSITTLGTVLAALAVLALWGPWELRHASPVVDLRVSARRQVLMTNLTSVVVGFAMYGMGLVIPQFLQLSAGTGYGLG
KSMVVAGLCFAPFGVVMMLASPLTARVSAMWGSKTTLVLGSAIIGVSYLIGLFVMHSVWQVVLLAVIGGIGVALAYASMP
SLIMAAVPATETAAANGLNTLMRSLGTSSSAAIVGVMLANMTITFGGREVPSLAGFHAVFALGAGAAAAAVLMGLFIPGR
GAREVPVPAVAAPRETGRPRAQLQQS

Sequences:

>Translated_506_residues
MARARTDPAEPGAGPASTAPMGPTAPRPSLLTAALAVAGIVASFMHTLVVPIIPQLPHLLDSSASNTTWVVTITLLAGAV
ATPVAGRLGDMYGKRRIMLGSVGLLGAGSLVCALSTSLPQMVVGRGLQGLATGLIPLGISLMRDELPPERLGSALGLMSS
SLGIGGALGVPTSALIAQNFSWHVLFWTATVLSVLVLAMLWRVVPESPVRDVRGRFDVVGALGLGTGITCLLLAISKGST
WGWTSITTLGTVLAALAVLALWGPWELRHASPVVDLRVSARRQVLMTNLTSVVVGFAMYGMGLVIPQFLQLSAGTGYGLG
KSMVVAGLCFAPFGVVMMLASPLTARVSAMWGSKTTLVLGSAIIGVSYLIGLFVMHSVWQVVLLAVIGGIGVALAYASMP
SLIMAAVPATETAAANGLNTLMRSLGTSSSAAIVGVMLANMTITFGGREVPSLAGFHAVFALGAGAAAAAVLMGLFIPGR
GAREVPVPAVAAPRETGRPRAQLQQS
>Mature_505_residues
ARARTDPAEPGAGPASTAPMGPTAPRPSLLTAALAVAGIVASFMHTLVVPIIPQLPHLLDSSASNTTWVVTITLLAGAVA
TPVAGRLGDMYGKRRIMLGSVGLLGAGSLVCALSTSLPQMVVGRGLQGLATGLIPLGISLMRDELPPERLGSALGLMSSS
LGIGGALGVPTSALIAQNFSWHVLFWTATVLSVLVLAMLWRVVPESPVRDVRGRFDVVGALGLGTGITCLLLAISKGSTW
GWTSITTLGTVLAALAVLALWGPWELRHASPVVDLRVSARRQVLMTNLTSVVVGFAMYGMGLVIPQFLQLSAGTGYGLGK
SMVVAGLCFAPFGVVMMLASPLTARVSAMWGSKTTLVLGSAIIGVSYLIGLFVMHSVWQVVLLAVIGGIGVALAYASMPS
LIMAAVPATETAAANGLNTLMRSLGTSSSAAIVGVMLANMTITFGGREVPSLAGFHAVFALGAGAAAAAVLMGLFIPGRG
AREVPVPAVAAPRETGRPRAQLQQS

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=435, Percent_Identity=27.5862068965517, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789042, Length=421, Percent_Identity=26.8408551068884, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=27.2289156626506, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=24.0425531914894, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081723, Length=127, Percent_Identity=35.4330708661417, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788392, Length=422, Percent_Identity=25.3554502369668, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51662; Mature: 51531

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARARTDPAEPGAGPASTAPMGPTAPRPSLLTAALAVAGIVASFMHTLVVPIIPQLPHLL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSSASNTTWVVTITLLAGAVATPVAGRLGDMYGKRRIMLGSVGLLGAGSLVCALSTSLPQ
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHCCHH
MVVGRGLQGLATGLIPLGISLMRDELPPERLGSALGLMSSSLGIGGALGVPTSALIAQNF
HHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
SWHVLFWTATVLSVLVLAMLWRVVPESPVRDVRGRFDVVGALGLGTGITCLLLAISKGST
CEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
WGWTSITTLGTVLAALAVLALWGPWELRHASPVVDLRVSARRQVLMTNLTSVVVGFAMYG
CCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLVIPQFLQLSAGTGYGLGKSMVVAGLCFAPFGVVMMLASPLTARVSAMWGSKTTLVLG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
SAIIGVSYLIGLFVMHSVWQVVLLAVIGGIGVALAYASMPSLIMAAVPATETAAANGLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LMRSLGTSSSAAIVGVMLANMTITFGGREVPSLAGFHAVFALGAGAAAAAVLMGLFIPGR
HHHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GAREVPVPAVAAPRETGRPRAQLQQS
CCCCCCCCCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure 
ARARTDPAEPGAGPASTAPMGPTAPRPSLLTAALAVAGIVASFMHTLVVPIIPQLPHLL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSSASNTTWVVTITLLAGAVATPVAGRLGDMYGKRRIMLGSVGLLGAGSLVCALSTSLPQ
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHCCHH
MVVGRGLQGLATGLIPLGISLMRDELPPERLGSALGLMSSSLGIGGALGVPTSALIAQNF
HHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCC
SWHVLFWTATVLSVLVLAMLWRVVPESPVRDVRGRFDVVGALGLGTGITCLLLAISKGST
CEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
WGWTSITTLGTVLAALAVLALWGPWELRHASPVVDLRVSARRQVLMTNLTSVVVGFAMYG
CCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGLVIPQFLQLSAGTGYGLGKSMVVAGLCFAPFGVVMMLASPLTARVSAMWGSKTTLVLG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
SAIIGVSYLIGLFVMHSVWQVVLLAVIGGIGVALAYASMPSLIMAAVPATETAAANGLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LMRSLGTSSSAAIVGVMLANMTITFGGREVPSLAGFHAVFALGAGAAAAAVLMGLFIPGR
HHHHHCCCCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
GAREVPVPAVAAPRETGRPRAQLQQS
CCCCCCCCCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]