Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 158315923

GI number: 158315923

Start: 4920577

End: 4922028

Strand: Reverse

Name: gatA [H]

Synonym: Franean1_4139

Alternate gene names: 158315923

Gene position: 4922028-4920577 (Counterclockwise)

Preceding gene: 158315924

Following gene: 158315920

Centisome position: 54.8

GC content: 71.01

Gene sequence:

>1452_bases
ATGGAGTGGAGTTTTCAGTCAGCCGAAGAGCTCGCGGCCGCGTTGCGTGCCGGTGACGTGACCTCGGCGGAGCTGACCGA
CGAGGCGATCGCCCGTATCGAGCGGGACGACAAGGTGATCAACGCGATCTGCGTGCCGGACTTCGACCGCGCGCGGGACG
CCGCGCGCGGCGCCGACCAGGCGCGCGCCCGCGGCGAGGACCGGCCGCTGCTCGGCATTCCGGTGACGGTCAAAGAGTCC
TACAACATCGCCGGGCTGCCCACGACCTGGGGCATGCCGCAGCACGCGAACTACCTGCCGGCCGAGGACGCGGTACAGGT
GTCGCGACTCAAGGCCGCAGGCGCGGTGGTGCTCGGCAAGACCAACGTGCCGTTGGGGCTGCAAGATATCCAGAGCTTCA
ACGAGATCTACGGCACCACCAACAACCCGTGGGATCACGATCGCACGCCGGGCGGCTCCTCCGGTGGATCGGCGGCGGCC
CTGGCGTCCGGGTTCGGCGCGCTGTCCATCGCCTCCGACATCGCCGGTTCGCTGCGCACCCCCGCGCATTTCTGCGGCGT
CTACGCGCACAAGCCGACACTCGGGCTGGCGGCGAGCCGCGGCATGGTCGCGCCTCCCGCGCCGGCATTGCCGACCGACC
TCGACCTCGCCGTCGTCGGTCCGATGGCGCGCACTGCCCGCGACCTCACCCTCCTGCTCGACGTCATGGCCGGACCGGAC
CCGCTGACGCTCGGCGTGGCGCACAACGTGGCGCTGCCGCCCGCGCGCCACGAGCGCCTCGCCGACTTCCGGGTCTTGGT
CCTCGACGAGCACCCGCTCATTCCGACCGGGTCCGCTGTGCGGGCGGGCGTGCACCAGGTGGCCGACGCGCTTGTCGACG
GAGGAGCCCGCGTCGAACGGCACACTCCGCTGCTGCCCGATCTGACCGAAGCCGCGACGCTCTACACGCAGTTGCTGTTC
TCGGGCTCCGTCGCGCGTTTTCCCGTCGAGGCGTACGAGCAGCTGCGGACCCGCGCCGCCGGGCTGAGCGCGGACGACCG
GAGTCTCGACGCGGCGCGGCTGCGCGCCATGGTGTTCAGCCACCGCGACTGGATCGAGGCGAACAACCGTCGCGAGCTCC
ACCGCCACGGCTGGCGGCAGCTGTTCGCCGAGTTCGACGTCGTGGTGTGTCCGATCACGCCGACTCCCGCGTTCGGTCAC
GACCACAACCCCAATCCGCTGGAACGCCGGATCGACATCGACGGCGTCGAGTACCCGTACTTCGACCAGCTCGTCTTGGC
CGGCCTGGCCACCATGCCGGGCCTGCCGGCCACCGCGATACCCGCGGGCCGGTCCCCCGAGGGCCTGCCGGTGGGAGTGC
AGCTCATCGGCCCGATGTTCGAGGACCGCACCCCGCTGCGGCTGGCCGAACTGCTCGAGCAGAAGATCGGCGGCTTCCAG
GCACCGACGTAG

Upstream 100 bases:

>100_bases
TTGTCTGTGGCCGGAGAGCAACATGATGAGTTGAGACATCTATCCTGTTGCGCGAATCGGCATTTCATGACCTATTTTAG
GGGCCGGTAGCGTCGGAATC

Downstream 100 bases:

>100_bases
GGCGTACCACAGGGTGTCCGTCGAGGACGAGGTGCGGATGACGAGCGAGGGCTGGGGCCTGGTCCTGATGCTCGCGGCCT
GGCACGCGGGACCGGCCATG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MEWSFQSAEELAAALRAGDVTSAELTDEAIARIERDDKVINAICVPDFDRARDAARGADQARARGEDRPLLGIPVTVKES
YNIAGLPTTWGMPQHANYLPAEDAVQVSRLKAAGAVVLGKTNVPLGLQDIQSFNEIYGTTNNPWDHDRTPGGSSGGSAAA
LASGFGALSIASDIAGSLRTPAHFCGVYAHKPTLGLAASRGMVAPPAPALPTDLDLAVVGPMARTARDLTLLLDVMAGPD
PLTLGVAHNVALPPARHERLADFRVLVLDEHPLIPTGSAVRAGVHQVADALVDGGARVERHTPLLPDLTEAATLYTQLLF
SGSVARFPVEAYEQLRTRAAGLSADDRSLDAARLRAMVFSHRDWIEANNRRELHRHGWRQLFAEFDVVVCPITPTPAFGH
DHNPNPLERRIDIDGVEYPYFDQLVLAGLATMPGLPATAIPAGRSPEGLPVGVQLIGPMFEDRTPLRLAELLEQKIGGFQ
APT

Sequences:

>Translated_483_residues
MEWSFQSAEELAAALRAGDVTSAELTDEAIARIERDDKVINAICVPDFDRARDAARGADQARARGEDRPLLGIPVTVKES
YNIAGLPTTWGMPQHANYLPAEDAVQVSRLKAAGAVVLGKTNVPLGLQDIQSFNEIYGTTNNPWDHDRTPGGSSGGSAAA
LASGFGALSIASDIAGSLRTPAHFCGVYAHKPTLGLAASRGMVAPPAPALPTDLDLAVVGPMARTARDLTLLLDVMAGPD
PLTLGVAHNVALPPARHERLADFRVLVLDEHPLIPTGSAVRAGVHQVADALVDGGARVERHTPLLPDLTEAATLYTQLLF
SGSVARFPVEAYEQLRTRAAGLSADDRSLDAARLRAMVFSHRDWIEANNRRELHRHGWRQLFAEFDVVVCPITPTPAFGH
DHNPNPLERRIDIDGVEYPYFDQLVLAGLATMPGLPATAIPAGRSPEGLPVGVQLIGPMFEDRTPLRLAELLEQKIGGFQ
APT
>Mature_483_residues
MEWSFQSAEELAAALRAGDVTSAELTDEAIARIERDDKVINAICVPDFDRARDAARGADQARARGEDRPLLGIPVTVKES
YNIAGLPTTWGMPQHANYLPAEDAVQVSRLKAAGAVVLGKTNVPLGLQDIQSFNEIYGTTNNPWDHDRTPGGSSGGSAAA
LASGFGALSIASDIAGSLRTPAHFCGVYAHKPTLGLAASRGMVAPPAPALPTDLDLAVVGPMARTARDLTLLLDVMAGPD
PLTLGVAHNVALPPARHERLADFRVLVLDEHPLIPTGSAVRAGVHQVADALVDGGARVERHTPLLPDLTEAATLYTQLLF
SGSVARFPVEAYEQLRTRAAGLSADDRSLDAARLRAMVFSHRDWIEANNRRELHRHGWRQLFAEFDVVVCPITPTPAFGH
DHNPNPLERRIDIDGVEYPYFDQLVLAGLATMPGLPATAIPAGRSPEGLPVGVQLIGPMFEDRTPLRLAELLEQKIGGFQ
APT

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=504, Percent_Identity=29.3650793650794, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI222831590, Length=515, Percent_Identity=25.631067961165, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI166795287, Length=156, Percent_Identity=39.1025641025641, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=503, Percent_Identity=26.8389662027833, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538252, Length=531, Percent_Identity=26.5536723163842, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=35.3711790393013, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71990152, Length=261, Percent_Identity=31.8007662835249, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=430, Percent_Identity=27.4418604651163, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=152, Percent_Identity=44.7368421052632, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=152, Percent_Identity=44.7368421052632, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=528, Percent_Identity=26.8939393939394, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=425, Percent_Identity=27.2941176470588, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=239, Percent_Identity=28.0334728033473, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644968, Length=516, Percent_Identity=28.6821705426357, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24648435, Length=498, Percent_Identity=27.9116465863454, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24648437, Length=498, Percent_Identity=27.9116465863454, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45550774, Length=498, Percent_Identity=27.9116465863454, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=27.7777777777778, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21356731, Length=502, Percent_Identity=27.6892430278884, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=28.0632411067194, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24648441, Length=440, Percent_Identity=27.0454545454545, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24648439, Length=440, Percent_Identity=27.0454545454545, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24648113, Length=520, Percent_Identity=27.8846153846154, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 51644; Mature: 51644

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWSFQSAEELAAALRAGDVTSAELTDEAIARIERDDKVINAICVPDFDRARDAARGADQ
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHH
ARARGEDRPLLGIPVTVKESYNIAGLPTTWGMPQHANYLPAEDAVQVSRLKAAGAVVLGK
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECC
TNVPLGLQDIQSFNEIYGTTNNPWDHDRTPGGSSGGSAAALASGFGALSIASDIAGSLRT
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCC
PAHFCGVYAHKPTLGLAASRGMVAPPAPALPTDLDLAVVGPMARTARDLTLLLDVMAGPD
CHHHHHHEECCCCCCHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCC
PLTLGVAHNVALPPARHERLADFRVLVLDEHPLIPTGSAVRAGVHQVADALVDGGARVER
CEEEEEECCCCCCCHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
HTPLLPDLTEAATLYTQLLFSGSVARFPVEAYEQLRTRAAGLSADDRSLDAARLRAMVFS
CCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
HRDWIEANNRRELHRHGWRQLFAEFDVVVCPITPTPAFGHDHNPNPLERRIDIDGVEYPY
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHEECCCCCCCCH
FDQLVLAGLATMPGLPATAIPAGRSPEGLPVGVQLIGPMFEDRTPLRLAELLEQKIGGFQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCHHHHHHHHHHHHCCCC
APT
CCC
>Mature Secondary Structure
MEWSFQSAEELAAALRAGDVTSAELTDEAIARIERDDKVINAICVPDFDRARDAARGADQ
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHH
ARARGEDRPLLGIPVTVKESYNIAGLPTTWGMPQHANYLPAEDAVQVSRLKAAGAVVLGK
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECC
TNVPLGLQDIQSFNEIYGTTNNPWDHDRTPGGSSGGSAAALASGFGALSIASDIAGSLRT
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCC
PAHFCGVYAHKPTLGLAASRGMVAPPAPALPTDLDLAVVGPMARTARDLTLLLDVMAGPD
CHHHHHHEECCCCCCHHCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCC
PLTLGVAHNVALPPARHERLADFRVLVLDEHPLIPTGSAVRAGVHQVADALVDGGARVER
CEEEEEECCCCCCCHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
HTPLLPDLTEAATLYTQLLFSGSVARFPVEAYEQLRTRAAGLSADDRSLDAARLRAMVFS
CCCCCCCHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
HRDWIEANNRRELHRHGWRQLFAEFDVVVCPITPTPAFGHDHNPNPLERRIDIDGVEYPY
HHHHHCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHEECCCCCCCCH
FDQLVLAGLATMPGLPATAIPAGRSPEGLPVGVQLIGPMFEDRTPLRLAELLEQKIGGFQ
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCHHHCCCCHHHHHHHHHHHHCCCC
APT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]