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The map label for this gene is cmcT [H]

Identifier: 158315864

GI number: 158315864

Start: 4861035

End: 4862447

Strand: Reverse

Name: cmcT [H]

Synonym: Franean1_4079

Alternate gene names: 158315864

Gene position: 4862447-4861035 (Counterclockwise)

Preceding gene: 158315866

Following gene: 158315863

Centisome position: 54.14

GC content: 73.25

Gene sequence:

>1413_bases
ATGACTCCGCGGCAGCGGCTGGCGCTCGTGCTGCTGCTGGGGACGCAGTTCATGCTCGCGATCGACTTCTCGATCCTCAA
CGTGGCCCTGCCGTCAATCGGGGCGGGGGTCGGCCTCGGTGAGGGTGACCTGCAGTGGATCGCGACCACGTTCGCGCTGC
CCTCCGCCGGGTTCACCCTGCTGTTCGCGCGGGTCGCCGACCTGGCCGGACGGCGCCGGATGTTCCTCACCGGCCTGGTG
CTGCTGATCGTGGCGTCCCTGCTCGGCGGGCTCGCCCAGGGGCCGACCGTGCTGCTGCTCGCCCGCGTCGGCCAGGGCCT
CGCCGCCGCGATCGCCATCCCGGCCGCGCTGTCCCTGCTGGTCACGACGTTCCCGGAGGGCCCCGTCCGGCAGCGTGCGC
TCAGCCTCAACGGTGCGCTGCTGCCCGCCGGCTTCACCGTGGGCGCGCTGGTGGGCGGGCTGCTGACCGGCTATCTCAGC
TGGCGCTGGGCCTTCCTCGTCAACGTTCCGGTGGCGCTGGCGATCCTGCTCGCCGCGCCGCGGGTCGTCCCCGGTGGCCG
CGCGGACGTCCGCCAGCGGCTCGACGTCCCCGGTGCGCTGACCGTCACCGCCGGGCTGGTCGCCTTCGTCTACGGCATCT
CCAGCGGCGGTGAGGACGGATGGGGCCGGCCGTCGGTGTGGATCGCGGTGGCCGCGGGGCTCGTCCTGCTCGGCGCCTTC
TGGCGGATAGAGGCGACCAGCCCGCATCCGCTGGCCTCGCTGGAGGTGCTGCGGCTGCGCCACGTCGGATGGGGCAACCT
CGGCGGGCTCGTGGTCTTCGCGATGGGCAGCTCGATCGTGTTCCTGATGACGCTGTACCTGCAGCGGGTCCTCGGCTACT
CGGCGGTGACGACCGGGCTGATCTTCGGGCTGCCCGGGACGGCCGCGTTCGTGGCCGGACTGGTGGCACCGCGCGTCCTG
GCCGTCGTGGAACCACGCCGCACCCTGGTGCTCGGGCTGCTCGTCCAGGGCCTGTCCGCGCTGGCCCTGGCCTTCGTCGG
GCTGGACCGCAGCTCGCTGTCGCTCGTGCTGGCGGCGTCCGTCGTCGGATTCTTCGGCCACGCCAGCGGGCTCGTCGCCT
ACCTGGTGACGGCGACCTCCGGGCTGCCCGACACCCAGCAGGGCCTGGCCACCGGCCTGACGACGATGACCCAGCAGGTG
GCACTCACACTGGGCATACCGATCATGAGCGCGGTGGCGACCGCCCGGACGTCCGCGCTCACCGCGGGTGGCGACCACTC
GGCGCGGGAGGCGACGCTGGGCGGCATCCAGACCGCGCTGGTCGTGAACGGGCTCGTCACGATCGCCCTGGCCGCACTGA
TCGCGACCGTCCTGCTCCGCCGCCGCCCGGGCCTCAGGACAGCTGTGGTGTAG

Upstream 100 bases:

>100_bases
GCACCCCGCACCGTCCGCCGCGGCCGAGCAGCCAGCCGCTGGGCAGCCCGTTGCCGGGCGGCCCGCCGCTGGGGATCCGC
GGCCAGCGGTGCCCGACCGG

Downstream 100 bases:

>100_bases
CGCGACCACGGGCCCGGGCTCCGGAGTGCCAGCGTGCCGGTGGATCAGCAAGCTTGGGGCGGCCCCGGGACCTTCCGTCA
GGCCCGGGGTTCCGCGCTCC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTPRQRLALVLLLGTQFMLAIDFSILNVALPSIGAGVGLGEGDLQWIATTFALPSAGFTLLFARVADLAGRRRMFLTGLV
LLIVASLLGGLAQGPTVLLLARVGQGLAAAIAIPAALSLLVTTFPEGPVRQRALSLNGALLPAGFTVGALVGGLLTGYLS
WRWAFLVNVPVALAILLAAPRVVPGGRADVRQRLDVPGALTVTAGLVAFVYGISSGGEDGWGRPSVWIAVAAGLVLLGAF
WRIEATSPHPLASLEVLRLRHVGWGNLGGLVVFAMGSSIVFLMTLYLQRVLGYSAVTTGLIFGLPGTAAFVAGLVAPRVL
AVVEPRRTLVLGLLVQGLSALALAFVGLDRSSLSLVLAASVVGFFGHASGLVAYLVTATSGLPDTQQGLATGLTTMTQQV
ALTLGIPIMSAVATARTSALTAGGDHSAREATLGGIQTALVVNGLVTIALAALIATVLLRRRPGLRTAVV

Sequences:

>Translated_470_residues
MTPRQRLALVLLLGTQFMLAIDFSILNVALPSIGAGVGLGEGDLQWIATTFALPSAGFTLLFARVADLAGRRRMFLTGLV
LLIVASLLGGLAQGPTVLLLARVGQGLAAAIAIPAALSLLVTTFPEGPVRQRALSLNGALLPAGFTVGALVGGLLTGYLS
WRWAFLVNVPVALAILLAAPRVVPGGRADVRQRLDVPGALTVTAGLVAFVYGISSGGEDGWGRPSVWIAVAAGLVLLGAF
WRIEATSPHPLASLEVLRLRHVGWGNLGGLVVFAMGSSIVFLMTLYLQRVLGYSAVTTGLIFGLPGTAAFVAGLVAPRVL
AVVEPRRTLVLGLLVQGLSALALAFVGLDRSSLSLVLAASVVGFFGHASGLVAYLVTATSGLPDTQQGLATGLTTMTQQV
ALTLGIPIMSAVATARTSALTAGGDHSAREATLGGIQTALVVNGLVTIALAALIATVLLRRRPGLRTAVV
>Mature_469_residues
TPRQRLALVLLLGTQFMLAIDFSILNVALPSIGAGVGLGEGDLQWIATTFALPSAGFTLLFARVADLAGRRRMFLTGLVL
LIVASLLGGLAQGPTVLLLARVGQGLAAAIAIPAALSLLVTTFPEGPVRQRALSLNGALLPAGFTVGALVGGLLTGYLSW
RWAFLVNVPVALAILLAAPRVVPGGRADVRQRLDVPGALTVTAGLVAFVYGISSGGEDGWGRPSVWIAVAAGLVLLGAFW
RIEATSPHPLASLEVLRLRHVGWGNLGGLVVFAMGSSIVFLMTLYLQRVLGYSAVTTGLIFGLPGTAAFVAGLVAPRVLA
VVEPRRTLVLGLLVQGLSALALAFVGLDRSSLSLVLAASVVGFFGHASGLVAYLVTATSGLPDTQQGLATGLTTMTQQVA
LTLGIPIMSAVATARTSALTAGGDHSAREATLGGIQTALVVNGLVTIALAALIATVLLRRRPGLRTAVV

Specific function: Involved in cephamycin export [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=405, Percent_Identity=28.3950617283951, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081983, Length=290, Percent_Identity=28.2758620689655, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788710, Length=416, Percent_Identity=23.7980769230769, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=24.0875912408759, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=294, Percent_Identity=29.2517006802721, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=391, Percent_Identity=21.9948849104859, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323520, Length=426, Percent_Identity=22.7699530516432, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323935, Length=412, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48121; Mature: 47990

Theoretical pI: Translated: 12.01; Mature: 12.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRQRLALVLLLGTQFMLAIDFSILNVALPSIGAGVGLGEGDLQWIATTFALPSAGFTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
LFARVADLAGRRRMFLTGLVLLIVASLLGGLAQGPTVLLLARVGQGLAAAIAIPAALSLL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHH
VTTFPEGPVRQRALSLNGALLPAGFTVGALVGGLLTGYLSWRWAFLVNVPVALAILLAAP
HHHCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVVPGGRADVRQRLDVPGALTVTAGLVAFVYGISSGGEDGWGRPSVWIAVAAGLVLLGAF
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
WRIEATSPHPLASLEVLRLRHVGWGNLGGLVVFAMGSSIVFLMTLYLQRVLGYSAVTTGL
HEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IFGLPGTAAFVAGLVAPRVLAVVEPRRTLVLGLLVQGLSALALAFVGLDRSSLSLVLAAS
HCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVGFFGHASGLVAYLVTATSGLPDTQQGLATGLTTMTQQVALTLGIPIMSAVATARTSAL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TAGGDHSAREATLGGIQTALVVNGLVTIALAALIATVLLRRRPGLRTAVV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TPRQRLALVLLLGTQFMLAIDFSILNVALPSIGAGVGLGEGDLQWIATTFALPSAGFTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHH
LFARVADLAGRRRMFLTGLVLLIVASLLGGLAQGPTVLLLARVGQGLAAAIAIPAALSLL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHH
VTTFPEGPVRQRALSLNGALLPAGFTVGALVGGLLTGYLSWRWAFLVNVPVALAILLAAP
HHHCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVVPGGRADVRQRLDVPGALTVTAGLVAFVYGISSGGEDGWGRPSVWIAVAAGLVLLGAF
CCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
WRIEATSPHPLASLEVLRLRHVGWGNLGGLVVFAMGSSIVFLMTLYLQRVLGYSAVTTGL
HEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IFGLPGTAAFVAGLVAPRVLAVVEPRRTLVLGLLVQGLSALALAFVGLDRSSLSLVLAAS
HCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VVGFFGHASGLVAYLVTATSGLPDTQQGLATGLTTMTQQVALTLGIPIMSAVATARTSAL
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
TAGGDHSAREATLGGIQTALVVNGLVTIALAALIATVLLRRRPGLRTAVV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8440253 [H]