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The map label for this gene is nifV [H]

Identifier: 158315856

GI number: 158315856

Start: 4853713

End: 4854954

Strand: Reverse

Name: nifV [H]

Synonym: Franean1_4071

Alternate gene names: 158315856

Gene position: 4854954-4853713 (Counterclockwise)

Preceding gene: 158315857

Following gene: 158315854

Centisome position: 54.05

GC content: 76.65

Gene sequence:

>1242_bases
ATGAAGAGCGGCACGTCGTCCTGCCCGCCCGCGCCGCGCCCCGTCCCGCCGGATCACGCGCGGGAGAACGGGTCCGGGCC
GGAACCCGGGCCTGGCCCCGGTCCGGTCCGGTTCTGCGACACCACCCTGCGTGACGGCGAGCAGACCCCGGGGGTCGCCT
TCACCGCCAAGGAGAAGATCGCCATCGCGGTCGCGCTCGACGCCGCCGGCGTGCACCAGATCGAGGCCGGGGTGCCGGCG
ATGGGGCCGGTCGAGGTGGATGTCCTGCGCCGCGTCGTCGCGGCGGTCGAGCGGGCCGGCGTGGTCGCCTGGTGCCGCGC
GGACCGCCGCGACGTCGACGCCGCCGTCGCCAGCGGGGTCGACGCCGCGCACCTGACCATCCCCGCCTCCGACCTTCACC
TGCGCACCAAGCTCGGCAAGGACCGGGCCTGGGCGCGGGCCCGCATCCGCGACTGCGTCCTCGACGCCACCGACCGCGGC
CTGCGGGTGAGCGTCGGCTTCGAGGACGCCTCCCGCGCGGACGACGGCTTCGTCATCGACCTGGCCGGGGAGCTACGGCG
CCTCGGCGTCACCCGGTTCCGCTGGGCGGACACGGTCGGGGTGGCCAATCCGATCACCCTGCACACGCGGCTGCGAGCGC
TGCTCGACGCCGTCCCCGGGCCGTGGGAGATCCACGCCCACGACGACTTCGGGCTGGCGACCGCCAACACGATCGCCGCG
GTGCAGGCCGGGTTCACCTGGGTGAGCACCACGGTGGCCGGCCTGGGCGAGCGTGCCGGCAACGCGCCGACCGAGGAGGT
CGCGATGGCGCTGCGGCACCTGCTCGGCCTGCCCGTCGACCTGGACACCGCCGCGTTCCGCCCGCTGGCGCGGCTGGTCG
CCGGCGCGTCGCGCCGGCCGGTTCCCGCCGGCAAGGCCGTGGTCGGCGACGCCGTGTTCGACCACGAGTCCGGCATCCAC
GTGCACGGCGTGCTGCGCGCCCCGGCGACCTACGAGCCGTTCGACCCGGCGGAGGTCGGCGCGCGCCGGCGGCTGGTGCT
GGGCAAGCACAGCGGCCGCGCCGCCGTGCGGCACGCGATGGACCGGCACGGCATCGACGCGCCCGACGAGGACCTGGAAC
CGATCGTCGGCCTGGTCCGCGCGCACGCCACCGTGTACAAGCAGCCGCTGAGTTCCGACCAGCTGCGGGCGATGGCCCGG
CGGGTCGCCACCCGCCGCGGCGCACGGCCCCGCCGCGGCTGA

Upstream 100 bases:

>100_bases
CCTGGAACCGTGCGTCTGCCTGTATGAGCCGGCCCGCGAGACCGGGAACACACAGCCAGCGCCGGATTCTCCTCAGCCGT
CGAAAGTGCCGGTGACCATC

Downstream 100 bases:

>100_bases
CCCGCCGCCGCTGGTGGCGCCACCGGCGGCGGCCCGCGCCGCCGGTCGCGCCTCGGCGGTCGGCCGCGGCGCGGTGGCGG
TCAGGGCCCGGGGCGGAAGG

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKSGTSSCPPAPRPVPPDHARENGSGPEPGPGPGPVRFCDTTLRDGEQTPGVAFTAKEKIAIAVALDAAGVHQIEAGVPA
MGPVEVDVLRRVVAAVERAGVVAWCRADRRDVDAAVASGVDAAHLTIPASDLHLRTKLGKDRAWARARIRDCVLDATDRG
LRVSVGFEDASRADDGFVIDLAGELRRLGVTRFRWADTVGVANPITLHTRLRALLDAVPGPWEIHAHDDFGLATANTIAA
VQAGFTWVSTTVAGLGERAGNAPTEEVAMALRHLLGLPVDLDTAAFRPLARLVAGASRRPVPAGKAVVGDAVFDHESGIH
VHGVLRAPATYEPFDPAEVGARRRLVLGKHSGRAAVRHAMDRHGIDAPDEDLEPIVGLVRAHATVYKQPLSSDQLRAMAR
RVATRRGARPRRG

Sequences:

>Translated_413_residues
MKSGTSSCPPAPRPVPPDHARENGSGPEPGPGPGPVRFCDTTLRDGEQTPGVAFTAKEKIAIAVALDAAGVHQIEAGVPA
MGPVEVDVLRRVVAAVERAGVVAWCRADRRDVDAAVASGVDAAHLTIPASDLHLRTKLGKDRAWARARIRDCVLDATDRG
LRVSVGFEDASRADDGFVIDLAGELRRLGVTRFRWADTVGVANPITLHTRLRALLDAVPGPWEIHAHDDFGLATANTIAA
VQAGFTWVSTTVAGLGERAGNAPTEEVAMALRHLLGLPVDLDTAAFRPLARLVAGASRRPVPAGKAVVGDAVFDHESGIH
VHGVLRAPATYEPFDPAEVGARRRLVLGKHSGRAAVRHAMDRHGIDAPDEDLEPIVGLVRAHATVYKQPLSSDQLRAMAR
RVATRRGARPRRG
>Mature_413_residues
MKSGTSSCPPAPRPVPPDHARENGSGPEPGPGPGPVRFCDTTLRDGEQTPGVAFTAKEKIAIAVALDAAGVHQIEAGVPA
MGPVEVDVLRRVVAAVERAGVVAWCRADRRDVDAAVASGVDAAHLTIPASDLHLRTKLGKDRAWARARIRDCVLDATDRG
LRVSVGFEDASRADDGFVIDLAGELRRLGVTRFRWADTVGVANPITLHTRLRALLDAVPGPWEIHAHDDFGLATANTIAA
VQAGFTWVSTTVAGLGERAGNAPTEEVAMALRHLLGLPVDLDTAAFRPLARLVAGASRRPVPAGKAVVGDAVFDHESGIH
VHGVLRAPATYEPFDPAEVGARRRLVLGKHSGRAAVRHAMDRHGIDAPDEDLEPIVGLVRAHATVYKQPLSSDQLRAMAR
RVATRRGARPRRG

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=356, Percent_Identity=34.5505617977528, Blast_Score=172, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320071, Length=390, Percent_Identity=27.6923076923077, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=373, Percent_Identity=27.8820375335121, Blast_Score=150, Evalue=4e-37,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 43801; Mature: 43801

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSGTSSCPPAPRPVPPDHARENGSGPEPGPGPGPVRFCDTTLRDGEQTPGVAFTAKEKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCE
AIAVALDAAGVHQIEAGVPAMGPVEVDVLRRVVAAVERAGVVAWCRADRRDVDAAVASGV
EEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCC
DAAHLTIPASDLHLRTKLGKDRAWARARIRDCVLDATDRGLRVSVGFEDASRADDGFVID
CCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEE
LAGELRRLGVTRFRWADTVGVANPITLHTRLRALLDAVPGPWEIHAHDDFGLATANTIAA
ECHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
VQAGFTWVSTTVAGLGERAGNAPTEEVAMALRHLLGLPVDLDTAAFRPLARLVAGASRRP
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
VPAGKAVVGDAVFDHESGIHVHGVLRAPATYEPFDPAEVGARRRLVLGKHSGRAAVRHAM
CCCCCCEECCHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHH
DRHGIDAPDEDLEPIVGLVRAHATVYKQPLSSDQLRAMARRVATRRGARPRRG
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSGTSSCPPAPRPVPPDHARENGSGPEPGPGPGPVRFCDTTLRDGEQTPGVAFTAKEKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCE
AIAVALDAAGVHQIEAGVPAMGPVEVDVLRRVVAAVERAGVVAWCRADRRDVDAAVASGV
EEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCC
DAAHLTIPASDLHLRTKLGKDRAWARARIRDCVLDATDRGLRVSVGFEDASRADDGFVID
CCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEE
LAGELRRLGVTRFRWADTVGVANPITLHTRLRALLDAVPGPWEIHAHDDFGLATANTIAA
ECHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHH
VQAGFTWVSTTVAGLGERAGNAPTEEVAMALRHLLGLPVDLDTAAFRPLARLVAGASRRP
HHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCC
VPAGKAVVGDAVFDHESGIHVHGVLRAPATYEPFDPAEVGARRRLVLGKHSGRAAVRHAM
CCCCCCEECCHHHCCCCCEEEEEEEECCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHH
DRHGIDAPDEDLEPIVGLVRAHATVYKQPLSSDQLRAMARRVATRRGARPRRG
HHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA