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The map label for this gene is polA [H]

Identifier: 158315552

GI number: 158315552

Start: 4453077

End: 4455098

Strand: Reverse

Name: polA [H]

Synonym: Franean1_3759

Alternate gene names: 158315552

Gene position: 4455098-4453077 (Counterclockwise)

Preceding gene: 158315554

Following gene: 158315550

Centisome position: 49.6

GC content: 78.73

Gene sequence:

>2022_bases
GTGGTCCCCACTTCCCGTCCCGTCGGCACGCCGGCCGCCGCGGGCGCCGGCTCTGGTGCCGCAGCTGGCTCTGGTGCCGC
GGCTGGCGCTGGGGCCGGCGCCGTGGCCGCGCTGCGGGCGGCCGGAGTGGGCCGGGGCGGCCTGGTGGCGGTGGTCCCGG
TCTCGGATGTCGCGGTGGCGCTGGCGTGCGCCGGCGCCGTCTGGGCCCAGCCCGCCGACCCGGCCGCCGTCCTGGCCGAA
GTGGAGTGCGCCCTCGCCCCCCGGTGGGTCTGGTGGTCGGCGCACAGCACCGGGGCACTGCTGACCAGGTCAGGTGCCAG
CACGATCGCCGGACCGCGCACTGGCTCTGGCGCCTACGCCGGCCCCGGCACCCACGCCGGCCCCGGCGCTGGCACCGGTC
CCGGGCGCAGTCCCATTCCCGCCGTGTGCTGGGATCTCGCGGCGGCGCACCGCGTCCTGTACGGGGGGCACCGGGATGAT
CCGGCGGCGGTCTGGGCCGGCGCACGCGGACTGCCGCTCCCGCCGCCACCGCCCCGGCCCCGCGCCGACGGCCAGCTCGA
CCTGCTCGCCCTGGTCGATCACCCGGGCGGCCCGGGTGACGGCGAGGCCGACGATCCGATCGGCCCGGACGGCCAGCTCC
GACCGGGCTGGGCCGAGGAGACGCTGCGCGGCGCGGGCCCGGGCGGGACGGGTGCCCAGCTGGCCCGGGCCACCCGGCTC
GCCCAGCTGGCCCTCACCGTCACGGCTGCTCAGGACGAGGCGCTGCGCGCACTGCCCGACCCGCGGGCGCGGCCGGCCGG
GCTCCCGCTGGCCGTCCTGACCGCGCGGGCCGAGTCCGGCGCCGAGCTCCTCGCCCGCGAACTGCGGCGGGACGGCCTGC
CGGTGGACCGGCCGACCGCCGAGGCGACGATCGCCGCCGCTGTCGGGCCGAGGCCCGACGGCGACGCCGCGGGCGCGGCT
GCCCGGCAGGCCCGGGACGCCCCCGTCCTGCGCCACCTCGGCACGTCGGTCGACCTGCGCAACCCGGTGGCGGTCCGGTC
CGCGCTCGCCGCGGTGGGCATCGACGTGCCGGACACCCGGTCGGGCCGGCTGAGCGCGCTGCGCGGTGTCCACCCTGTCG
TCGAGGCGCTGCTCGCCTGGCGCAAGGCCGAGCGGATCGCGACGACCTACGGGTACCGGTGGCTCGACACCCATGTCGGC
GCCGACGGCCGGCTACGGGGCGAGTGGCGCGGCAGCGACGGCGCCGCCGGCCGGATGACCGCGGCCGCCGGCCTGCACAA
CCTGCCGGCCGACCTGCGGGTCGTGGTGATCGCCGAGCCGGGGTACCGGTTCGTCCGCGCCGACCTCGGCCAGATCGAGC
CGCGGGTGCTGGCCGTGGTCTCCGGCGACCCGGAGCTGACCAGGGCTACCCGGCAGGACGACCTCTACGCCCCGGTCGCG
GCCAGCCTCGGCGTCGACCGGCCGACGGCGAAGGTCGCGGTGCTCGCTGCGATGTACGGGCAGACGACCGGCGTCGCCGG
CCAGGCCCTGCGGCAGATGCGCGAGACCTACCCGGTCGCGCTGGCGTACCTGGACGCCGCCGACGCCACGGGCCGGGCCG
CCGCTCTGGGCCGGCCACCGCCGAACCCACCGGTCACAGACCCACAGGCGCGGCTGCGGACGTTCGGCGGGCGGCGGGTC
CCGCTGCGGTTCGCCGCGGTGGACGACGGCGCCGACGAGAACAGGGTGGGTATGACCTGGGGCCGGTTCGCGCGCAACGC
CGTCGTGCAGGGCGCGGCCGCCGAGCTGTTCAAGGCGTGGGCCGTGACGGTACGCCAGGCGATCGCCCCGCTCGGCGGCG
CGATCGTCCTCTGCCTGCACGACGAGCTGCTGCTGCATGTGCCGGCCCCGCGGGCGGAGGCGGCGGCGGAGGCGACCCGC
CGGGCGCTGGACGCCACCGTCGGATACTGGGCCGCCGGCAGCGGGGTCCGTTTCGTCGTCGACATCGCGGTCGTGCGGCG
GTGGTCCGATGCCAAGGCCTGA

Upstream 100 bases:

>100_bases
AGCAGGCGGTGTGCGCGCCGGAATCTGGCATGCGTAGATCCCGCACCCGTCCCGGGCCCGTCCCGGGATTTCTGTCAGTG
CGGCGGGAGAGGATGACCGC

Downstream 100 bases:

>100_bases
CACCCGCCCGCGCGGCGTCGCGAGCAGGCGAAGCCCTCGGCGGCCTGGCGTAGCACCCGAGGAGTGACCGAAAGGCTACT
GTATGGCTTCGGCGCTGCGT

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: TFI polymerase 1 [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MVPTSRPVGTPAAAGAGSGAAAGSGAAAGAGAGAVAALRAAGVGRGGLVAVVPVSDVAVALACAGAVWAQPADPAAVLAE
VECALAPRWVWWSAHSTGALLTRSGASTIAGPRTGSGAYAGPGTHAGPGAGTGPGRSPIPAVCWDLAAAHRVLYGGHRDD
PAAVWAGARGLPLPPPPPRPRADGQLDLLALVDHPGGPGDGEADDPIGPDGQLRPGWAEETLRGAGPGGTGAQLARATRL
AQLALTVTAAQDEALRALPDPRARPAGLPLAVLTARAESGAELLARELRRDGLPVDRPTAEATIAAAVGPRPDGDAAGAA
ARQARDAPVLRHLGTSVDLRNPVAVRSALAAVGIDVPDTRSGRLSALRGVHPVVEALLAWRKAERIATTYGYRWLDTHVG
ADGRLRGEWRGSDGAAGRMTAAAGLHNLPADLRVVVIAEPGYRFVRADLGQIEPRVLAVVSGDPELTRATRQDDLYAPVA
ASLGVDRPTAKVAVLAAMYGQTTGVAGQALRQMRETYPVALAYLDAADATGRAAALGRPPPNPPVTDPQARLRTFGGRRV
PLRFAAVDDGADENRVGMTWGRFARNAVVQGAAAELFKAWAVTVRQAIAPLGGAIVLCLHDELLLHVPAPRAEAAAEATR
RALDATVGYWAAGSGVRFVVDIAVVRRWSDAKA

Sequences:

>Translated_673_residues
MVPTSRPVGTPAAAGAGSGAAAGSGAAAGAGAGAVAALRAAGVGRGGLVAVVPVSDVAVALACAGAVWAQPADPAAVLAE
VECALAPRWVWWSAHSTGALLTRSGASTIAGPRTGSGAYAGPGTHAGPGAGTGPGRSPIPAVCWDLAAAHRVLYGGHRDD
PAAVWAGARGLPLPPPPPRPRADGQLDLLALVDHPGGPGDGEADDPIGPDGQLRPGWAEETLRGAGPGGTGAQLARATRL
AQLALTVTAAQDEALRALPDPRARPAGLPLAVLTARAESGAELLARELRRDGLPVDRPTAEATIAAAVGPRPDGDAAGAA
ARQARDAPVLRHLGTSVDLRNPVAVRSALAAVGIDVPDTRSGRLSALRGVHPVVEALLAWRKAERIATTYGYRWLDTHVG
ADGRLRGEWRGSDGAAGRMTAAAGLHNLPADLRVVVIAEPGYRFVRADLGQIEPRVLAVVSGDPELTRATRQDDLYAPVA
ASLGVDRPTAKVAVLAAMYGQTTGVAGQALRQMRETYPVALAYLDAADATGRAAALGRPPPNPPVTDPQARLRTFGGRRV
PLRFAAVDDGADENRVGMTWGRFARNAVVQGAAAELFKAWAVTVRQAIAPLGGAIVLCLHDELLLHVPAPRAEAAAEATR
RALDATVGYWAAGSGVRFVVDIAVVRRWSDAKA
>Mature_673_residues
MVPTSRPVGTPAAAGAGSGAAAGSGAAAGAGAGAVAALRAAGVGRGGLVAVVPVSDVAVALACAGAVWAQPADPAAVLAE
VECALAPRWVWWSAHSTGALLTRSGASTIAGPRTGSGAYAGPGTHAGPGAGTGPGRSPIPAVCWDLAAAHRVLYGGHRDD
PAAVWAGARGLPLPPPPPRPRADGQLDLLALVDHPGGPGDGEADDPIGPDGQLRPGWAEETLRGAGPGGTGAQLARATRL
AQLALTVTAAQDEALRALPDPRARPAGLPLAVLTARAESGAELLARELRRDGLPVDRPTAEATIAAAVGPRPDGDAAGAA
ARQARDAPVLRHLGTSVDLRNPVAVRSALAAVGIDVPDTRSGRLSALRGVHPVVEALLAWRKAERIATTYGYRWLDTHVG
ADGRLRGEWRGSDGAAGRMTAAAGLHNLPADLRVVVIAEPGYRFVRADLGQIEPRVLAVVSGDPELTRATRQDDLYAPVA
ASLGVDRPTAKVAVLAAMYGQTTGVAGQALRQMRETYPVALAYLDAADATGRAAALGRPPPNPPVTDPQARLRTFGGRRV
PLRFAAVDDGADENRVGMTWGRFARNAVVQGAAAELFKAWAVTVRQAIAPLGGAIVLCLHDELLLHVPAPRAEAAAEATR
RALDATVGYWAAGSGVRFVVDIAVVRRWSDAKA

Specific function: Has 5'-to-3' exonuclease activity and no 3'-to-5' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI86563272, Length=321, Percent_Identity=25.8566978193146, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337
- InterPro:   IPR015361 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A; PF09281 Taq-exonuc [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 68515; Mature: 68515

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPTSRPVGTPAAAGAGSGAAAGSGAAAGAGAGAVAALRAAGVGRGGLVAVVPVSDVAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
LACAGAVWAQPADPAAVLAEVECALAPRWVWWSAHSTGALLTRSGASTIAGPRTGSGAYA
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCC
GPGTHAGPGAGTGPGRSPIPAVCWDLAAAHRVLYGGHRDDPAAVWAGARGLPLPPPPPRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCC
RADGQLDLLALVDHPGGPGDGEADDPIGPDGQLRPGWAEETLRGAGPGGTGAQLARATRL
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AQLALTVTAAQDEALRALPDPRARPAGLPLAVLTARAESGAELLARELRRDGLPVDRPTA
HHHHEEEECCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
EATIAAAVGPRPDGDAAGAAARQARDAPVLRHLGTSVDLRNPVAVRSALAAVGIDVPDTR
CEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
SGRLSALRGVHPVVEALLAWRKAERIATTYGYRWLDTHVGADGRLRGEWRGSDGAAGRMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCCCCEEECEECCCCCCCCCHH
AAAGLHNLPADLRVVVIAEPGYRFVRADLGQIEPRVLAVVSGDPELTRATRQDDLYAPVA
HHHCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCHHH
ASLGVDRPTAKVAVLAAMYGQTTGVAGQALRQMRETYPVALAYLDAADATGRAAALGRPP
HHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCC
PNPPVTDPQARLRTFGGRRVPLRFAAVDDGADENRVGMTWGRFARNAVVQGAAAELFKAW
CCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVTVRQAIAPLGGAIVLCLHDELLLHVPAPRAEAAAEATRRALDATVGYWAAGSGVRFVV
HHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
DIAVVRRWSDAKA
EHHHHHHCCCCCC
>Mature Secondary Structure
MVPTSRPVGTPAAAGAGSGAAAGSGAAAGAGAGAVAALRAAGVGRGGLVAVVPVSDVAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHH
LACAGAVWAQPADPAAVLAEVECALAPRWVWWSAHSTGALLTRSGASTIAGPRTGSGAYA
HHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCC
GPGTHAGPGAGTGPGRSPIPAVCWDLAAAHRVLYGGHRDDPAAVWAGARGLPLPPPPPRP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCC
RADGQLDLLALVDHPGGPGDGEADDPIGPDGQLRPGWAEETLRGAGPGGTGAQLARATRL
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHH
AQLALTVTAAQDEALRALPDPRARPAGLPLAVLTARAESGAELLARELRRDGLPVDRPTA
HHHHEEEECCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCC
EATIAAAVGPRPDGDAAGAAARQARDAPVLRHLGTSVDLRNPVAVRSALAAVGIDVPDTR
CEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCC
SGRLSALRGVHPVVEALLAWRKAERIATTYGYRWLDTHVGADGRLRGEWRGSDGAAGRMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCCCCEEECEECCCCCCCCCHH
AAAGLHNLPADLRVVVIAEPGYRFVRADLGQIEPRVLAVVSGDPELTRATRQDDLYAPVA
HHHCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCHHH
ASLGVDRPTAKVAVLAAMYGQTTGVAGQALRQMRETYPVALAYLDAADATGRAAALGRPP
HHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCC
PNPPVTDPQARLRTFGGRRVPLRFAAVDDGADENRVGMTWGRFARNAVVQGAAAELFKAW
CCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVTVRQAIAPLGGAIVLCLHDELLLHVPAPRAEAAAEATRRALDATVGYWAAGSGVRFVV
HHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
DIAVVRRWSDAKA
EHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9509419 [H]