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The map label for this gene is yogA [H]

Identifier: 158315531

GI number: 158315531

Start: 4425279

End: 4426226

Strand: Reverse

Name: yogA [H]

Synonym: Franean1_3738

Alternate gene names: 158315531

Gene position: 4426226-4425279 (Counterclockwise)

Preceding gene: 158315533

Following gene: 158315530

Centisome position: 49.28

GC content: 73.52

Gene sequence:

>948_bases
ATGCGCGCACTGAATGTCCCGGCCGCGGGCGAGCAGCCCCGGCTGTCTGACCTGCCTGTCCCCGGGCCCGTCGAGGGCAC
CGTGCTGGTCCGTGTGAAGGCAGCCGGTCTCAACGCCATCGACAACGCCCTGGCCGCCGGAATGATGGCCGAGATGCTGC
CGCACCTATACCCGCTGGTCCTCGGTCGTGACGCCGCCGGCGTCGTCGAGGCCGTCGGAGCCGGCGTCGACCACGTCGCC
GTCGGCGACGAGGTGATCGGCCACATGCTGCTGGTCCCACCGATTCAGGCCGGCACCCTCGCCGAGTACGCACTGCTGCC
CGCTGCCGCCGTCACCCGCAAGCCCGCCGGCCTCGACTTCACCACCGCCGCGGCACTCCCCCTCGCCGCAGCCGCCGCCA
AGACGGCCGTCGACGCCGTCGAGCCTGAGCCGGGTCAGACTGTTCTCGTCGTCGGGGCCGGCGGCGGCGTGGGTTCGTAC
GCCGTCCAGCTGCTCGCGGCACGCGACGTCACCGTCGTGGCCACCGGCACCGCCGCCGACGCCGGCCGGCTGACCGCGCT
GGGCGCGACCACCGTCGTCGATTACACCGCCGGCCCGGTCGCCGAACAGGTGCGCGCCGCCTACCCCGACGGTGTCGACG
CCCTGATCGACCTCGTCGCCTACACCCCTGACAGTGCGCCTCTGGACGTGGTCCGCAAGGGCGGCAAGGTCGCCAGCACC
CTGGGCGCCGCCGACGAAGAGGCCCTCGCCGCCGCCGGCCTGACCGGCACCAGCGTCATGGCCGCCCCCGTCCGCGAGCT
CATCGCGGAGCTTGCCGCCCTGGCTGCCGCCGGCACCCTGAAGGTCGACATCACCACCGTCCTGCCCCTCGAGAAGGCCA
CCGACGGGCTGGCCACTCTCGCCAGCGGTGCCGCCCGCGGAAAGATCGTCGTCGAGATCGGCGACTGA

Upstream 100 bases:

>100_bases
GCTTCAACTCACAGACTGGGTGTCCGAGGCTGCGAGTTTGGCCGCGACGCTCACCCACCCACCGGGGTCGCCATCGATCC
CGATCCCCTAGGGAGCACAC

Downstream 100 bases:

>100_bases
CAGGACGCGCCCCATAACCACTGACGGGAACACCCGCGGAGACACACCGAACCGTAGGCGACCAACATGTACGGCAGAGA
CAGGAGATAAGACGGGTGCC

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRALNVPAAGEQPRLSDLPVPGPVEGTVLVRVKAAGLNAIDNALAAGMMAEMLPHLYPLVLGRDAAGVVEAVGAGVDHVA
VGDEVIGHMLLVPPIQAGTLAEYALLPAAAVTRKPAGLDFTTAAALPLAAAAAKTAVDAVEPEPGQTVLVVGAGGGVGSY
AVQLLAARDVTVVATGTAADAGRLTALGATTVVDYTAGPVAEQVRAAYPDGVDALIDLVAYTPDSAPLDVVRKGGKVAST
LGAADEEALAAAGLTGTSVMAAPVRELIAELAALAAAGTLKVDITTVLPLEKATDGLATLASGAARGKIVVEIGD

Sequences:

>Translated_315_residues
MRALNVPAAGEQPRLSDLPVPGPVEGTVLVRVKAAGLNAIDNALAAGMMAEMLPHLYPLVLGRDAAGVVEAVGAGVDHVA
VGDEVIGHMLLVPPIQAGTLAEYALLPAAAVTRKPAGLDFTTAAALPLAAAAAKTAVDAVEPEPGQTVLVVGAGGGVGSY
AVQLLAARDVTVVATGTAADAGRLTALGATTVVDYTAGPVAEQVRAAYPDGVDALIDLVAYTPDSAPLDVVRKGGKVAST
LGAADEEALAAAGLTGTSVMAAPVRELIAELAALAAAGTLKVDITTVLPLEKATDGLATLASGAARGKIVVEIGD
>Mature_315_residues
MRALNVPAAGEQPRLSDLPVPGPVEGTVLVRVKAAGLNAIDNALAAGMMAEMLPHLYPLVLGRDAAGVVEAVGAGVDHVA
VGDEVIGHMLLVPPIQAGTLAEYALLPAAAVTRKPAGLDFTTAAALPLAAAAAKTAVDAVEPEPGQTVLVVGAGGGVGSY
AVQLLAARDVTVVATGTAADAGRLTALGATTVVDYTAGPVAEQVRAAYPDGVDALIDLVAYTPDSAPLDVVRKGGKVAST
LGAADEEALAAAGLTGTSVMAAPVRELIAELAALAAAGTLKVDITTVLPLEKATDGLATLASGAARGKIVVEIGD

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=327, Percent_Identity=30.2752293577982, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI194239674, Length=327, Percent_Identity=29.6636085626911, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI13236495, Length=327, Percent_Identity=29.6636085626911, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI18379349, Length=314, Percent_Identity=28.6624203821656, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI24308257, Length=224, Percent_Identity=31.6964285714286, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI194239676, Length=320, Percent_Identity=28.4375, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI28557745, Length=208, Percent_Identity=28.3653846153846, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790485, Length=304, Percent_Identity=27.6315789473684, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71987554, Length=370, Percent_Identity=28.1081081081081, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17507255, Length=224, Percent_Identity=34.375, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=221, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=324, Percent_Identity=26.2345679012346, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581341, Length=376, Percent_Identity=25, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581339, Length=376, Percent_Identity=25, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 30984; Mature: 30984

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALNVPAAGEQPRLSDLPVPGPVEGTVLVRVKAAGLNAIDNALAAGMMAEMLPHLYPLV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LGRDAAGVVEAVGAGVDHVAVGDEVIGHMLLVPPIQAGTLAEYALLPAAAVTRKPAGLDF
HCCCHHHHHHHHCCCCCEEECCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
TTAAALPLAAAAAKTAVDAVEPEPGQTVLVVGAGGGVGSYAVQLLAARDVTVVATGTAAD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCC
AGRLTALGATTVVDYTAGPVAEQVRAAYPDGVDALIDLVAYTPDSAPLDVVRKGGKVAST
CCCEEEECCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
LGAADEEALAAAGLTGTSVMAAPVRELIAELAALAAAGTLKVDITTVLPLEKATDGLATL
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECHHHHCCHHHHH
ASGAARGKIVVEIGD
HCCCCCCEEEEEECC
>Mature Secondary Structure
MRALNVPAAGEQPRLSDLPVPGPVEGTVLVRVKAAGLNAIDNALAAGMMAEMLPHLYPLV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LGRDAAGVVEAVGAGVDHVAVGDEVIGHMLLVPPIQAGTLAEYALLPAAAVTRKPAGLDF
HCCCHHHHHHHHCCCCCEEECCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
TTAAALPLAAAAAKTAVDAVEPEPGQTVLVVGAGGGVGSYAVQLLAARDVTVVATGTAAD
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCC
AGRLTALGATTVVDYTAGPVAEQVRAAYPDGVDALIDLVAYTPDSAPLDVVRKGGKVAST
CCCEEEECCCEEEECCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHH
LGAADEEALAAAGLTGTSVMAAPVRELIAELAALAAAGTLKVDITTVLPLEKATDGLATL
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECHHHHCCHHHHH
ASGAARGKIVVEIGD
HCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]