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The map label for this gene is stp [H]

Identifier: 158315353

GI number: 158315353

Start: 4218201

End: 4219784

Strand: Direct

Name: stp [H]

Synonym: Franean1_3555

Alternate gene names: 158315353

Gene position: 4218201-4219784 (Clockwise)

Preceding gene: 158315352

Following gene: 158315358

Centisome position: 46.96

GC content: 73.36

Gene sequence:

>1584_bases
ATGCGTAAATGGCTGACCCTGATCACGGTCTCCCTGAGCACCTTCATGCTGCTGCTCGACGTCACGATCGTCAGCGTCGC
GGTGCCGGCGATGGCCCGCGCGCTCGACTCCTCATTCACTGATCTGCAGTGGACCGTCGACATCTACGTCCTGGTGCTCG
CCGCGCTTCTGATGGCGATCGGGTCGGCGTCCGACCTCCTCGGCCGCCGCAAGGTCTTCCTGCTCGGGCTGGTCGTCTTC
GCGGCGGCCTCCCTCGCCTGCGGGCTGGCCCCGAACACCGGCTTCCTCATCGCCGCCCGGGGGGTGCAGGGGCTCGGCGC
CGCGGCCATGTTCGCCACCAACGCCGCGCTGCTCAGCGCCACCTACCGCGGCCGCGACGTCGGTGTCGCCTTCGGGGTGT
GGGGCGCGGTCAACGGCGCCGCCGCGGCGCTCGGCCCGATCGTCGGCGGCCTGCTCACCGAGCACGTCAGCTGGCGGGCC
ATCTTCCTGGTCAACCTGCCGGTCGCGCTGATCGCCATCGTGATCGCCCTGCGCTCCGTCGCCGAATCGCGGGACCGGAT
GAGCGGCCGGATCGACATCCCCGGCACCGTCACGTTCACGCTCACCGTCTCGCTGCTCATCTACGGCCTCATCGAGGCCG
GTGACAAGGGCTGGTCGGATTCGGTCACCCTCGGGTGCCTCGCCGGCGCCGCCGTGGCCCTGGTGGTCTTCGTCCTGGTG
GAACGGGGCCGGCGCGCTCCCATGCTCGAGCCGCGGCTGTTCCGCGGCCCATCGTTCTCCGCGCTCATGGTCGGCGGCTT
CGTGCTGACCGGGGCAGCCTTCGCGAACCTGGTGTTCGTGTCGGTGTGGGCGCAGACCGTCCTCGACTTCGACCCGGTGA
AGGCCGGGCTCGTGCTCACCCCGTTGGCGGGGGTCTCGTTCGTGGTCGCCGGCGCCGGCGGCCGGCTGCTGCACGGCGTG
CCGCCGAGGTACTCGATCGGGGCGGGCCTGCTGCTGGTCGGGGTCGGTACGTTCCTCGACATGATCATCGCTCCGTCGTC
GGGATGGACCGCGCTGCTGGCCGGACTGATCGTCACCGGCGTCGGGGTGGGGCTGGCCTCGCCGGCGCTCGCCTCCGCAG
CGCTCACCACGGTGCCCCCCGAGCGCGCGGGGATGGCCAACGGCGCCATGAACACGTTCCGCCAGCTCGGGTTCGCCGTC
GGCATCCCCGTCTTCGGCACGGCCCTGGCCGGGCAGGCCCGGGCCAGCCTCAGCGACAGCGGCCAGTTCGACGACCCGCA
GGCCACCGCCAGCGCGCTGTCCGGCGGCGGCGCGCCGGAGATCATCGCCCACGTCCCCGCGGCCGCTCGCGCCGCGGTCG
ACCAGGCCCTGCACGCCGCGTTCGCCGCCGGCCTCGACCGTGTCTTCCTGATCAGCGGCATCGCCGGGGTGGTAGCCGGT
GCCGTGGTGCTGCTGCTCGTCCGACCGGAACAGGCGGCCGCCCGCGCCGCGGCGGACGACGGCCCGGCGGACGCGGTGCC
CGGAGGCCCCGCGATCCCGTCGCCCGGGGACGGCAGCCAGGTGCCGACGGGGGCGAACGGCTGA

Upstream 100 bases:

>100_bases
GCGGGCCGCTAGGAACCCGAGGTCACTCCCGCCACCGCCCGCGGGACGCCGTCGGGCCGACCCCCCGAGCGCCGCCTCGC
CCTCCCGAGAACGGTTCCCC

Downstream 100 bases:

>100_bases
GGAGGGGCCGCGGTCAGGGCCCGGGAGGTGGGGCGGCGAGCAGCCCGTCCCGCAGCTCCCGGACCTGGTCGGCCACCGCG
CCGACGGCACCCCGGTCGTG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MRKWLTLITVSLSTFMLLLDVTIVSVAVPAMARALDSSFTDLQWTVDIYVLVLAALLMAIGSASDLLGRRKVFLLGLVVF
AAASLACGLAPNTGFLIAARGVQGLGAAAMFATNAALLSATYRGRDVGVAFGVWGAVNGAAAALGPIVGGLLTEHVSWRA
IFLVNLPVALIAIVIALRSVAESRDRMSGRIDIPGTVTFTLTVSLLIYGLIEAGDKGWSDSVTLGCLAGAAVALVVFVLV
ERGRRAPMLEPRLFRGPSFSALMVGGFVLTGAAFANLVFVSVWAQTVLDFDPVKAGLVLTPLAGVSFVVAGAGGRLLHGV
PPRYSIGAGLLLVGVGTFLDMIIAPSSGWTALLAGLIVTGVGVGLASPALASAALTTVPPERAGMANGAMNTFRQLGFAV
GIPVFGTALAGQARASLSDSGQFDDPQATASALSGGGAPEIIAHVPAAARAAVDQALHAAFAAGLDRVFLISGIAGVVAG
AVVLLLVRPEQAAARAAADDGPADAVPGGPAIPSPGDGSQVPTGANG

Sequences:

>Translated_527_residues
MRKWLTLITVSLSTFMLLLDVTIVSVAVPAMARALDSSFTDLQWTVDIYVLVLAALLMAIGSASDLLGRRKVFLLGLVVF
AAASLACGLAPNTGFLIAARGVQGLGAAAMFATNAALLSATYRGRDVGVAFGVWGAVNGAAAALGPIVGGLLTEHVSWRA
IFLVNLPVALIAIVIALRSVAESRDRMSGRIDIPGTVTFTLTVSLLIYGLIEAGDKGWSDSVTLGCLAGAAVALVVFVLV
ERGRRAPMLEPRLFRGPSFSALMVGGFVLTGAAFANLVFVSVWAQTVLDFDPVKAGLVLTPLAGVSFVVAGAGGRLLHGV
PPRYSIGAGLLLVGVGTFLDMIIAPSSGWTALLAGLIVTGVGVGLASPALASAALTTVPPERAGMANGAMNTFRQLGFAV
GIPVFGTALAGQARASLSDSGQFDDPQATASALSGGGAPEIIAHVPAAARAAVDQALHAAFAAGLDRVFLISGIAGVVAG
AVVLLLVRPEQAAARAAADDGPADAVPGGPAIPSPGDGSQVPTGANG
>Mature_527_residues
MRKWLTLITVSLSTFMLLLDVTIVSVAVPAMARALDSSFTDLQWTVDIYVLVLAALLMAIGSASDLLGRRKVFLLGLVVF
AAASLACGLAPNTGFLIAARGVQGLGAAAMFATNAALLSATYRGRDVGVAFGVWGAVNGAAAALGPIVGGLLTEHVSWRA
IFLVNLPVALIAIVIALRSVAESRDRMSGRIDIPGTVTFTLTVSLLIYGLIEAGDKGWSDSVTLGCLAGAAVALVVFVLV
ERGRRAPMLEPRLFRGPSFSALMVGGFVLTGAAFANLVFVSVWAQTVLDFDPVKAGLVLTPLAGVSFVVAGAGGRLLHGV
PPRYSIGAGLLLVGVGTFLDMIIAPSSGWTALLAGLIVTGVGVGLASPALASAALTTVPPERAGMANGAMNTFRQLGFAV
GIPVFGTALAGQARASLSDSGQFDDPQATASALSGGGAPEIIAHVPAAARAAVDQALHAAFAAGLDRVFLISGIAGVVAG
AVVLLLVRPEQAAARAAADDGPADAVPGGPAIPSPGDGSQVPTGANG

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=405, Percent_Identity=29.3827160493827, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789042, Length=444, Percent_Identity=25.4504504504505, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788710, Length=425, Percent_Identity=26.1176470588235, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790195, Length=398, Percent_Identity=26.3819095477387, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=429, Percent_Identity=28.6713286713287, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI48994889, Length=128, Percent_Identity=33.59375, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=388, Percent_Identity=25.2577319587629, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=312, Percent_Identity=25.6410256410256, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=458, Percent_Identity=24.4541484716157, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=448, Percent_Identity=23.2142857142857, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=155, Percent_Identity=27.0967741935484, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=28.1879194630872, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52997; Mature: 52997

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKWLTLITVSLSTFMLLLDVTIVSVAVPAMARALDSSFTDLQWTVDIYVLVLAALLMAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GSASDLLGRRKVFLLGLVVFAAASLACGLAPNTGFLIAARGVQGLGAAAMFATNAALLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
TYRGRDVGVAFGVWGAVNGAAAALGPIVGGLLTEHVSWRAIFLVNLPVALIAIVIALRSV
HCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHH
AESRDRMSGRIDIPGTVTFTLTVSLLIYGLIEAGDKGWSDSVTLGCLAGAAVALVVFVLV
HHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERGRRAPMLEPRLFRGPSFSALMVGGFVLTGAAFANLVFVSVWAQTVLDFDPVKAGLVLT
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
PLAGVSFVVAGAGGRLLHGVPPRYSIGAGLLLVGVGTFLDMIIAPSSGWTALLAGLIVTG
CCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGVGLASPALASAALTTVPPERAGMANGAMNTFRQLGFAVGIPVFGTALAGQARASLSDS
HHHHHCCHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
GQFDDPQATASALSGGGAPEIIAHVPAAARAAVDQALHAAFAAGLDRVFLISGIAGVVAG
CCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVVLLLVRPEQAAARAAADDGPADAVPGGPAIPSPGDGSQVPTGANG
HHHHHEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKWLTLITVSLSTFMLLLDVTIVSVAVPAMARALDSSFTDLQWTVDIYVLVLAALLMAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GSASDLLGRRKVFLLGLVVFAAASLACGLAPNTGFLIAARGVQGLGAAAMFATNAALLSA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
TYRGRDVGVAFGVWGAVNGAAAALGPIVGGLLTEHVSWRAIFLVNLPVALIAIVIALRSV
HCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHH
AESRDRMSGRIDIPGTVTFTLTVSLLIYGLIEAGDKGWSDSVTLGCLAGAAVALVVFVLV
HHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ERGRRAPMLEPRLFRGPSFSALMVGGFVLTGAAFANLVFVSVWAQTVLDFDPVKAGLVLT
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
PLAGVSFVVAGAGGRLLHGVPPRYSIGAGLLLVGVGTFLDMIIAPSSGWTALLAGLIVTG
CCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VGVGLASPALASAALTTVPPERAGMANGAMNTFRQLGFAVGIPVFGTALAGQARASLSDS
HHHHHCCHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
GQFDDPQATASALSGGGAPEIIAHVPAAARAAVDQALHAAFAAGLDRVFLISGIAGVVAG
CCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AVVLLLVRPEQAAARAAADDGPADAVPGGPAIPSPGDGSQVPTGANG
HHHHHEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]