BacMap

Definition	Frankia sp. EAN1pec chromosome, complete genome.
Accession	NC_009921
Length	8,982,042

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The map label for this gene is lcfB [H]

Identifier: 158315291

GI number: 158315291

Start: 4153548

End: 4154951

Strand: Reverse

Name: lcfB [H]

Synonym: Franean1_3491

Alternate gene names: 158315291

Gene position: 4154951-4153548 (Counterclockwise)

Preceding gene: 158315294

Following gene: 158315290

Centisome position: 46.26

GC content: 72.51

Gene sequence:

>1404_bases
GTGCCACCTTCGTATCCAGAGGCAAGCCACTGCAGCGAGCCAGCAAGCCGGGACTCGACTGTTCCGTCGACCGAACCGGG
TGGCGGGCCCGACAATGCGAGGGTGCTGACTGCATTGCGTCAAGGCCGGGCCGTGGTCCGGATGTCGGACGCCGCGCTCG
CGGGCGATGATCTGGCCGGCGCGGTGGGCGCCGTCGCCGAGCGGCTACGGGGGCTGCGCCGGATCGCGGTGGTGGCCACT
CCGACGTTGCAGACCGTGGTCGCGATCGCCGGGGCGGTGGAGGCCGGCGTCACCGTGGTCACGATCAACCCGCAGGCCGG
CGACGCCGAGCGGGCGTACGTCCTGGCCGACAGCGACCCCGACCTGCTGCTCGACGACGTGGATCTCATGCAGCGCGGCG
CGCTGCCGAGCGAGCCGGCCGATGACGACGCACCCGCGCTGGTCATCTACACCTCCGGGACCACCGGGCCCCCGAAGGGC
GCGGTGATTCCGCGCCGCGCGGTCGCGTCCAACATCGACGCGCTGGCCAACGCGTGGGCCTGGTCGCCGGACGATGTGCT
CGGGCACGCGCTGCCGCTGTTCCACGTGCACGGGCTGGTGCTCGGGACGCTGGGCCCGCTGCGGATCGGGTCCGCGCTGC
ACCATGTGGCGCGGTTCGCGCCGGTGCCGGGCGCGACGTTGTACTTCGCCGTGCCGACCATGTGGTCCCGGGTGCCCGAG
CCCGAGGCGTTCGCGTCCGCCCGGCTGCTCGTGTCGGGTTCGGCGGCGCTGCCCGTGCCGGTGTTCGACCGGTTCGCCGC
ACGCGCCGGCCAGCGGGTCGCCGAACGTTACGGGCTCACCGAGACCCTCATCAACACCGCCGCGCGGGCCGACGGCGACC
GCCGTCCGGGCCTGGTGGGTGCGCCGCTGGGCGGCGTCGAGGTCCGGGTCACCGGCGTCACCGGCGACGACGAGTTCGGA
CCGGTGGAGGTGCGCGGCCCGAACGTCTTCACCGGCTACCTGGGCAACCCGGCCGCCACCGCCGCGGCGCTGACACCCGA
CGGCTGGTTCTCCACCGGAGACCTCGGCCTCTTCGAGCCGGACGGGCAGCTGCGCATCGTCGGACGGCAGAGCACCGACC
TCATCAAGTCGGGTGGGTACAAGATCGGAGCCGGTGAGGTCGAGAACGCGCTGCTGGCCCATCCGTCCGTCGCCGAGGCC
GCGGTCGTCGGCGTGCCCGACGACGATCTCGGCCAGCGCATCGTGGCGGTTGTCATCACGCACGAGCCGATCGACGCCAC
GACGTTGATCGACCATGTCGCGACCGCGCTGGCCCCGCACAAGCGCCCGCGCGAGATCCGGTTCGCCACCGAACTGCCGC
GCAACCAGATGGGCAAGGTGCAGAAGAAAAAGCTGCTCACCTGA

Upstream 100 bases:

>100_bases
TGACGTCCTTCCAGTTCAACTCTGACACTTTGATATATCAGGTGTCAAGACGGCGGCAGGGATGGCCAATGGCACGCCCG
GCGCCGTGGGGCGTTGCGGG

Downstream 100 bases:

>100_bases
TCACCGGTCGCTGAAGAGTTACCGGACGGCCCGCGCTCGACGATGCCGACCCGCTGTTCCGGAAAGCGAAGTACGCCGGC
ACTCCCCGCGTGACGCGGTC

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPPSYPEASHCSEPASRDSTVPSTEPGGGPDNARVLTALRQGRAVVRMSDAALAGDDLAGAVGAVAERLRGLRRIAVVAT
PTLQTVVAIAGAVEAGVTVVTINPQAGDAERAYVLADSDPDLLLDDVDLMQRGALPSEPADDDAPALVIYTSGTTGPPKG
AVIPRRAVASNIDALANAWAWSPDDVLGHALPLFHVHGLVLGTLGPLRIGSALHHVARFAPVPGATLYFAVPTMWSRVPE
PEAFASARLLVSGSAALPVPVFDRFAARAGQRVAERYGLTETLINTAARADGDRRPGLVGAPLGGVEVRVTGVTGDDEFG
PVEVRGPNVFTGYLGNPAATAAALTPDGWFSTGDLGLFEPDGQLRIVGRQSTDLIKSGGYKIGAGEVENALLAHPSVAEA
AVVGVPDDDLGQRIVAVVITHEPIDATTLIDHVATALAPHKRPREIRFATELPRNQMGKVQKKKLLT

Sequences:

>Translated_467_residues
MPPSYPEASHCSEPASRDSTVPSTEPGGGPDNARVLTALRQGRAVVRMSDAALAGDDLAGAVGAVAERLRGLRRIAVVAT
PTLQTVVAIAGAVEAGVTVVTINPQAGDAERAYVLADSDPDLLLDDVDLMQRGALPSEPADDDAPALVIYTSGTTGPPKG
AVIPRRAVASNIDALANAWAWSPDDVLGHALPLFHVHGLVLGTLGPLRIGSALHHVARFAPVPGATLYFAVPTMWSRVPE
PEAFASARLLVSGSAALPVPVFDRFAARAGQRVAERYGLTETLINTAARADGDRRPGLVGAPLGGVEVRVTGVTGDDEFG
PVEVRGPNVFTGYLGNPAATAAALTPDGWFSTGDLGLFEPDGQLRIVGRQSTDLIKSGGYKIGAGEVENALLAHPSVAEA
AVVGVPDDDLGQRIVAVVITHEPIDATTLIDHVATALAPHKRPREIRFATELPRNQMGKVQKKKLLT
>Mature_466_residues
PPSYPEASHCSEPASRDSTVPSTEPGGGPDNARVLTALRQGRAVVRMSDAALAGDDLAGAVGAVAERLRGLRRIAVVATP
TLQTVVAIAGAVEAGVTVVTINPQAGDAERAYVLADSDPDLLLDDVDLMQRGALPSEPADDDAPALVIYTSGTTGPPKGA
VIPRRAVASNIDALANAWAWSPDDVLGHALPLFHVHGLVLGTLGPLRIGSALHHVARFAPVPGATLYFAVPTMWSRVPEP
EAFASARLLVSGSAALPVPVFDRFAARAGQRVAERYGLTETLINTAARADGDRRPGLVGAPLGGVEVRVTGVTGDDEFGP
VEVRGPNVFTGYLGNPAATAAALTPDGWFSTGDLGLFEPDGQLRIVGRQSTDLIKSGGYKIGAGEVENALLAHPSVAEAA
VVGVPDDDLGQRIVAVVITHEPIDATTLIDHVATALAPHKRPREIRFATELPRNQMGKVQKKKLLT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=517, Percent_Identity=34.0425531914894, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI187761343, Length=517, Percent_Identity=34.0425531914894, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI42544132, Length=349, Percent_Identity=29.512893982808, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI157311624, Length=232, Percent_Identity=35.7758620689655, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI157311622, Length=232, Percent_Identity=35.7758620689655, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI58082049, Length=359, Percent_Identity=28.9693593314763, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI122937307, Length=353, Percent_Identity=28.6118980169972, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI115511026, Length=442, Percent_Identity=26.4705882352941, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI42794760, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI42794758, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI42794756, Length=420, Percent_Identity=27.1428571428571, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI38505220, Length=355, Percent_Identity=30.1408450704225, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI156151445, Length=354, Percent_Identity=28.8135593220339, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI40807491, Length=382, Percent_Identity=29.3193717277487, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI57165410, Length=346, Percent_Identity=28.3236994219653, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI57165412, Length=346, Percent_Identity=28.3236994219653, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI13375727, Length=374, Percent_Identity=25.668449197861, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI28416953, Length=123, Percent_Identity=32.520325203252, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693145, Length=349, Percent_Identity=30.6590257879656, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788107, Length=344, Percent_Identity=28.4883720930233, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI221142682, Length=354, Percent_Identity=28.2485875706215, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1786810, Length=356, Percent_Identity=29.4943820224719, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789201, Length=354, Percent_Identity=27.9661016949153, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788595, Length=317, Percent_Identity=31.8611987381703, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786801, Length=471, Percent_Identity=23.1422505307856, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790505, Length=387, Percent_Identity=24.2894056847545, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786529, Length=386, Percent_Identity=27.4611398963731, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=444, Percent_Identity=32.8828828828829, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI32563687, Length=434, Percent_Identity=32.7188940092166, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17559526, Length=373, Percent_Identity=27.6139410187668, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=357, Percent_Identity=29.1316526610644, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=377, Percent_Identity=28.6472148541114, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=377, Percent_Identity=28.6472148541114, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=346, Percent_Identity=26.878612716763, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=397, Percent_Identity=28.9672544080605, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=395, Percent_Identity=26.5822784810127, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=394, Percent_Identity=26.1421319796954, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=368, Percent_Identity=23.9130434782609, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25141359, Length=476, Percent_Identity=23.3193277310924, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=414, Percent_Identity=24.8792270531401, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17510401, Length=177, Percent_Identity=31.0734463276836, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25147511, Length=378, Percent_Identity=28.042328042328, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556552, Length=342, Percent_Identity=25.7309941520468, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=24.5179063360882, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17541856, Length=164, Percent_Identity=35.3658536585366, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204819, Length=162, Percent_Identity=34.5679012345679, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=342, Percent_Identity=32.1637426900585, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6323182, Length=388, Percent_Identity=27.0618556701031, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323903, Length=322, Percent_Identity=25.4658385093168, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322182, Length=331, Percent_Identity=26.2839879154079, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324893, Length=334, Percent_Identity=26.6467065868263, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=384, Percent_Identity=23.4375, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320852, Length=179, Percent_Identity=28.4916201117318, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859661, Length=504, Percent_Identity=25.5952380952381, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355181, Length=373, Percent_Identity=27.3458445040214, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24581924, Length=369, Percent_Identity=28.7262872628726, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=412, Percent_Identity=28.1553398058252, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24656500, Length=368, Percent_Identity=29.0760869565217, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24666501, Length=184, Percent_Identity=35.3260869565217, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24666497, Length=184, Percent_Identity=35.3260869565217, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281366413, Length=184, Percent_Identity=35.3260869565217, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=24.7863247863248, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356947, Length=232, Percent_Identity=29.7413793103448, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358303, Length=216, Percent_Identity=27.3148148148148, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648257, Length=416, Percent_Identity=24.5192307692308, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356441, Length=323, Percent_Identity=25.077399380805, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130357, Length=342, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI281365686, Length=291, Percent_Identity=23.3676975945017, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=358, Percent_Identity=22.9050279329609, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648255, Length=319, Percent_Identity=24.4514106583072, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648260, Length=341, Percent_Identity=25.2199413489736, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648253, Length=319, Percent_Identity=24.4514106583072, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 48533; Mature: 48401

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSYPEASHCSEPASRDSTVPSTEPGGGPDNARVLTALRQGRAVVRMSDAALAGDDLAG
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHCCCHHHH
AVGAVAERLRGLRRIAVVATPTLQTVVAIAGAVEAGVTVVTINPQAGDAERAYVLADSDP
HHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCC
DLLLDDVDLMQRGALPSEPADDDAPALVIYTSGTTGPPKGAVIPRRAVASNIDALANAWA
CEEEHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
WSPDDVLGHALPLFHVHGLVLGTLGPLRIGSALHHVARFAPVPGATLYFAVPTMWSRVPE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCC
PEAFASARLLVSGSAALPVPVFDRFAARAGQRVAERYGLTETLINTAARADGDRRPGLVG
CHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEE
APLGGVEVRVTGVTGDDEFGPVEVRGPNVFTGYLGNPAATAAALTPDGWFSTGDLGLFEP
CCCCCEEEEEEEECCCCCCCCEEEECCCEEEECCCCCHHCEEEECCCCCCCCCCCCEECC
DGQLRIVGRQSTDLIKSGGYKIGAGEVENALLAHPSVAEAAVVGVPDDDLGQRIVAVVIT
CCCEEEEECCCHHHHHCCCEEECCCHHHHHHHCCCCHHCEEEEECCCHHHCCEEEEEEEE
HEPIDATTLIDHVATALAPHKRPREIRFATELPRNQMGKVQKKKLLT
CCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PPSYPEASHCSEPASRDSTVPSTEPGGGPDNARVLTALRQGRAVVRMSDAALAGDDLAG
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHCCCHHHH
AVGAVAERLRGLRRIAVVATPTLQTVVAIAGAVEAGVTVVTINPQAGDAERAYVLADSDP
HHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEECCCC
DLLLDDVDLMQRGALPSEPADDDAPALVIYTSGTTGPPKGAVIPRRAVASNIDALANAWA
CEEEHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCC
WSPDDVLGHALPLFHVHGLVLGTLGPLRIGSALHHVARFAPVPGATLYFAVPTMWSRVPE
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHCCCC
PEAFASARLLVSGSAALPVPVFDRFAARAGQRVAERYGLTETLINTAARADGDRRPGLVG
CHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEE
APLGGVEVRVTGVTGDDEFGPVEVRGPNVFTGYLGNPAATAAALTPDGWFSTGDLGLFEP
CCCCCEEEEEEEECCCCCCCCEEEECCCEEEECCCCCHHCEEEECCCCCCCCCCCCEECC
DGQLRIVGRQSTDLIKSGGYKIGAGEVENALLAHPSVAEAAVVGVPDDDLGQRIVAVVIT
CCCEEEEECCCHHHHHCCCEEECCCHHHHHHHCCCCHHCEEEEECCCHHHCCEEEEEEEE
HEPIDATTLIDHVATALAPHKRPREIRFATELPRNQMGKVQKKKLLT
CCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]