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The map label for this gene is gsiB [H]

Identifier: 158315264

GI number: 158315264

Start: 4093947

End: 4095536

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_3464

Alternate gene names: 158315264

Gene position: 4095536-4093947 (Counterclockwise)

Preceding gene: 158315265

Following gene: 158315263

Centisome position: 45.6

GC content: 71.95

Gene sequence:

>1590_bases
ATGAGACGCGGACGCCCGCCAAGACCCCTCCGATCATTCTGGTCCGCCCGGCGGTTGCGGACCGCGGCGGCGGCCGCGCT
GGCCGCGCTGGCGGTCAGCACGGTCGCCGCCTGCGGCGGTTCCACCGCCGCCGACGGGACCGCGCCGGCGGCGCAGGGCG
GGACCCTCACCGTCCTGTGGCCCGCCGAACCACTCGACCTGTCCACCGACAGCGGCTTCGGACTACAGATGATCTCCGGA
TCGATCGAGCGCCTCGCCGTGTACGACGCCCTGGTCGCGATCACCCCGGCCGCCGAGCTGGACTACCGCCTGGCCACCTC
CCTCGACTCCGACGACTCGCTGACCTGGACGCTGCGGCTGCGCGAGGGGCTGCGGTTCAGCGACGGCACCCCGCTGGACG
CCGCCGCGGTCCGGGACAACTGGACCCTGCTGGCCGATCCGGCCCGCAAGTCACCCAGCGCCAAGATCGCACAGCGGATC
GGGTCATTCACCATCGTGGACCCGACAACCCTGCGGATCACGCTGAAGGAGGCCGACGGCCAGTTCCCCCGGCTGGTCGC
GCAGACCCCGCTGACCTTCATCGGCTCCCCCACCGCGCTGCGCGCCAAGGGCGACGGGTTCAAGACCGCGCCGGTCGGCG
CCGGGGCCTTCACCGTCCGGGAGTGGCTGCGCAACGACCACCTGACTCTGGTCCGCAACCCGACCTCGTCGGTCCAGGCG
CACTACGACACGATCGTCGTCAAGTCCGTCCCGGACGAGACGCAGCGCTACAACACCCTGCTCGCGGGGGGCGCGGACAT
CGCCTTCTCCGCGAACCTGCGCACCGGCATCACCGCGGTCGCGGCCGGGCTGGTCACCGAGAAGGCGTTCAGCGACGGCG
GGCTCAACCTGCTGTTCAACATCACCAAGGCCCCCTTCGACGACATCCGGGCCCGCCGGGCGGTCTCCTACGCCCTCGAC
GCGCAGGCGCTGAACAAGGCCCTGTTCGACGGGACCGCCGCCGTGCCGTCCAGCTTCCTGCGCGACGACTCGCCGCTGCA
CAGCGACGTGCCGCTGCCCCGCCCGGACCGGGCGAAGGCCCAGGCCCTGTTCGACGAGCTCGCCGCCGCGGGCAAGCCGG
TGCAGTTCACCATCATCTCGCCGCTGAACTTCAGCAACGTCGCCGAATGGGTGCAGTCCAGCCTCGGTGGCTTCCGGAAC
GTCAGCGTGAAGGTCGACGCGATGGCCCAGACGCTGCCCGTGCTCCAGGGCGGCTTCCAGGCCACGCTCACCGGCACCCC
GCGGTTCGTGGACCCCTACCCGCAGCTGGCCCTGAACCTGGGCACCGGCGGCCCGAGCAACTACGGCAAGTTCTCCGACC
CGGCCCTCGACGCCGCGCTGCGGGAGGGGCAGCAGTCCCGGGACACGGCCGTCCGGGTCCGGGCCTACGAGACCGCGCAG
CGGATCATCGCCGAACAGCTGCCGCTGGCCGGCCCGCTGTACCGCCTGCCGGGCCAGTACCTACACGCGTCCACGGCCTT
CGGTACGGGCAAGCTCCCGATCATCAACGACGGCGTGCTCGACATCACCCGGCTCACCGGGGCGGGGTGA

Upstream 100 bases:

>100_bases
ACTGAGCCCCGGCCCGCCGAGCCCCGGCCCGCCGGGGCACCCGCCCCACCCACCCGGCAGATCACCCACGATCACCTTGA
GCACCCGGAGCAGTTGAGCC

Downstream 100 bases:

>100_bases
TCCAGTGAGCGAGCACCGCGGCGGCACGGCCGTCCGGCTGGTCGTGCCGAGACTGCTGCGGGCCGCCGCGGTGCTGCTCC
TGGTCGGCCTGGGGGTCTCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MRRGRPPRPLRSFWSARRLRTAAAAALAALAVSTVAACGGSTAADGTAPAAQGGTLTVLWPAEPLDLSTDSGFGLQMISG
SIERLAVYDALVAITPAAELDYRLATSLDSDDSLTWTLRLREGLRFSDGTPLDAAAVRDNWTLLADPARKSPSAKIAQRI
GSFTIVDPTTLRITLKEADGQFPRLVAQTPLTFIGSPTALRAKGDGFKTAPVGAGAFTVREWLRNDHLTLVRNPTSSVQA
HYDTIVVKSVPDETQRYNTLLAGGADIAFSANLRTGITAVAAGLVTEKAFSDGGLNLLFNITKAPFDDIRARRAVSYALD
AQALNKALFDGTAAVPSSFLRDDSPLHSDVPLPRPDRAKAQALFDELAAAGKPVQFTIISPLNFSNVAEWVQSSLGGFRN
VSVKVDAMAQTLPVLQGGFQATLTGTPRFVDPYPQLALNLGTGGPSNYGKFSDPALDAALREGQQSRDTAVRVRAYETAQ
RIIAEQLPLAGPLYRLPGQYLHASTAFGTGKLPIINDGVLDITRLTGAG

Sequences:

>Translated_529_residues
MRRGRPPRPLRSFWSARRLRTAAAAALAALAVSTVAACGGSTAADGTAPAAQGGTLTVLWPAEPLDLSTDSGFGLQMISG
SIERLAVYDALVAITPAAELDYRLATSLDSDDSLTWTLRLREGLRFSDGTPLDAAAVRDNWTLLADPARKSPSAKIAQRI
GSFTIVDPTTLRITLKEADGQFPRLVAQTPLTFIGSPTALRAKGDGFKTAPVGAGAFTVREWLRNDHLTLVRNPTSSVQA
HYDTIVVKSVPDETQRYNTLLAGGADIAFSANLRTGITAVAAGLVTEKAFSDGGLNLLFNITKAPFDDIRARRAVSYALD
AQALNKALFDGTAAVPSSFLRDDSPLHSDVPLPRPDRAKAQALFDELAAAGKPVQFTIISPLNFSNVAEWVQSSLGGFRN
VSVKVDAMAQTLPVLQGGFQATLTGTPRFVDPYPQLALNLGTGGPSNYGKFSDPALDAALREGQQSRDTAVRVRAYETAQ
RIIAEQLPLAGPLYRLPGQYLHASTAFGTGKLPIINDGVLDITRLTGAG
>Mature_529_residues
MRRGRPPRPLRSFWSARRLRTAAAAALAALAVSTVAACGGSTAADGTAPAAQGGTLTVLWPAEPLDLSTDSGFGLQMISG
SIERLAVYDALVAITPAAELDYRLATSLDSDDSLTWTLRLREGLRFSDGTPLDAAAVRDNWTLLADPARKSPSAKIAQRI
GSFTIVDPTTLRITLKEADGQFPRLVAQTPLTFIGSPTALRAKGDGFKTAPVGAGAFTVREWLRNDHLTLVRNPTSSVQA
HYDTIVVKSVPDETQRYNTLLAGGADIAFSANLRTGITAVAAGLVTEKAFSDGGLNLLFNITKAPFDDIRARRAVSYALD
AQALNKALFDGTAAVPSSFLRDDSPLHSDVPLPRPDRAKAQALFDELAAAGKPVQFTIISPLNFSNVAEWVQSSLGGFRN
VSVKVDAMAQTLPVLQGGFQATLTGTPRFVDPYPQLALNLGTGGPSNYGKFSDPALDAALREGQQSRDTAVRVRAYETAQ
RIIAEQLPLAGPLYRLPGQYLHASTAFGTGKLPIINDGVLDITRLTGAG

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=500, Percent_Identity=27.6, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI87081878, Length=522, Percent_Identity=25.6704980842912, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787762, Length=400, Percent_Identity=24.5, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789887, Length=329, Percent_Identity=26.4437689969605, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56092; Mature: 56092

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGRPPRPLRSFWSARRLRTAAAAALAALAVSTVAACGGSTAADGTAPAAQGGTLTVLW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
PAEPLDLSTDSGFGLQMISGSIERLAVYDALVAITPAAELDYRLATSLDSDDSLTWTLRL
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHCCCCCCCCEEEEEEE
REGLRFSDGTPLDAAAVRDNWTLLADPARKSPSAKIAQRIGSFTIVDPTTLRITLKEADG
CCCCCCCCCCCCCHHEECCCCEEEECCCCCCCHHHHHHHHCCEEEECCCEEEEEEECCCC
QFPRLVAQTPLTFIGSPTALRAKGDGFKTAPVGAGAFTVREWLRNDHLTLVRNPTSSVQA
CCCHHHHHCCHHCCCCCCEEEECCCCCEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHE
HYDTIVVKSVPDETQRYNTLLAGGADIAFSANLRTGITAVAAGLVTEKAFSDGGLNLLFN
EEEEEEEECCCCHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITKAPFDDIRARRAVSYALDAQALNKALFDGTAAVPSSFLRDDSPLHSDVPLPRPDRAKA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCCCCCCCCCCHHHH
QALFDELAAAGKPVQFTIISPLNFSNVAEWVQSSLGGFRNVSVKVDAMAQTLPVLQGGFQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCCE
ATLTGTPRFVDPYPQLALNLGTGGPSNYGKFSDPALDAALREGQQSRDTAVRVRAYETAQ
EEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHH
RIIAEQLPLAGPLYRLPGQYLHASTAFGTGKLPIINDGVLDITRLTGAG
HHHHHHCCCCCCHHHCCCHHEEEECCCCCCCCCEEECCCEEEEECCCCC
>Mature Secondary Structure
MRRGRPPRPLRSFWSARRLRTAAAAALAALAVSTVAACGGSTAADGTAPAAQGGTLTVLW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEE
PAEPLDLSTDSGFGLQMISGSIERLAVYDALVAITPAAELDYRLATSLDSDDSLTWTLRL
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCHHCCCHHHCCCCCCCCEEEEEEE
REGLRFSDGTPLDAAAVRDNWTLLADPARKSPSAKIAQRIGSFTIVDPTTLRITLKEADG
CCCCCCCCCCCCCHHEECCCCEEEECCCCCCCHHHHHHHHCCEEEECCCEEEEEEECCCC
QFPRLVAQTPLTFIGSPTALRAKGDGFKTAPVGAGAFTVREWLRNDHLTLVRNPTSSVQA
CCCHHHHHCCHHCCCCCCEEEECCCCCEECCCCCCHHHHHHHHCCCCEEEEECCCHHHHE
HYDTIVVKSVPDETQRYNTLLAGGADIAFSANLRTGITAVAAGLVTEKAFSDGGLNLLFN
EEEEEEEECCCCHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITKAPFDDIRARRAVSYALDAQALNKALFDGTAAVPSSFLRDDSPLHSDVPLPRPDRAKA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCCCCCCCCCCHHHH
QALFDELAAAGKPVQFTIISPLNFSNVAEWVQSSLGGFRNVSVKVDAMAQTLPVLQGGFQ
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCCE
ATLTGTPRFVDPYPQLALNLGTGGPSNYGKFSDPALDAALREGQQSRDTAVRVRAYETAQ
EEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHH
RIIAEQLPLAGPLYRLPGQYLHASTAFGTGKLPIINDGVLDITRLTGAG
HHHHHHCCCCCCHHHCCCHHEEEECCCCCCCCCEEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA