Click here to switch to the map view.

The map label for this gene is insG [H]

Identifier: 158315229

GI number: 158315229

Start: 4059514

End: 4060779

Strand: Direct

Name: insG [H]

Synonym: Franean1_3429

Alternate gene names: 158315229

Gene position: 4059514-4060779 (Clockwise)

Preceding gene: 158315225

Following gene: 158315231

Centisome position: 45.2

GC content: 71.88

Gene sequence:

>1266_bases
GTGCCGAGACGCGGTCAGGTCAAGGAGAAGCCCGAGGACCGGTTGGTGGACCGGGTCGGGTTGGGGGTGCTGGCGGCGCA
GTTCCCGGACGCGCTGGTCGACCGGGTGGTCGCTGAGACCGGGCGGCGTGAGCGGCGGACGCGGGACCTGCCGGCGGCGT
TGACCCTGCGGTATGTGGTGGCGTTGGCGTTGTTCCCGTCGGACGGCTACGACGAGGTGATGCGGCAGGTGAAGGTCGCC
GACGACTGGCTGTCGGACAAGGCCGGCCCGGTGAAGGTGCCCGCGACCACGGCGATCACGAAGGCGCGGGACCGGCTCGG
GGTGGAGCCGGTGAAGCTGCTGTTCGAGCGCACGGCGGTGCCGATGGCCCTGCCCCGGCGGACGGTGGGGGCGTTCTACC
GGGGCTGGCGGGTGTGCACGGTCGACGGGACGACGCTGCTGGTCCCGGACACCGACGAGAACGCCGCCGCGTTCGGTAAG
CCCGGCAACGACCAGGGCGAGGGCGCGTTGCCGCAGGTCCGGGTGCTCGGGCTGGTCGAGTGCGGCACCCGGGCGCTGCT
GGGCGCCGGGTTCGGCGGGACCGGGGGGTCCAAGGCTGCCAGCGAGCAGGCCCTGTTCCCCGACCTGCTCGGCGCGTTGC
GCCCGGGCATGCTGGTGCTGGCCGACCGGAACTTCCTCGGCTTCGAGTTGTTCGCCAAGGCCGCCGCGACCGGCGCGGAC
CTGCTGTGGCGGGCCAAGTCCGACCGGCGCCTGCCCATCGACACCGAGCTCGCCGACGGCTCTTACCTGTCCCACCTCGT
CGAACCCGGCACCCGAGACAAAGGCCGGAAAATCACGGTACGGGTCGTCGAGTACACCCTCGACCGCGACCCCGACAGCC
CCCTGCCCGCGGGCAAGAAGGAGACCTACCGGCTGGTCACCACAATCCTCGATCCCGACGCCGCGCCCGCCACCGACCTG
GCCGCGCTCTACAGCGACCGGTGGGAAGTCGAGACCCTCCTCGACGAAATCAAGGTCCACCAGCAGGACGGCCGGCTGGT
GCTGCGGTCCCGGGCCCCCGACCGGGTCGAGCAGGAGGTCTGGGGCGTCCTGCTGCTGCACCGGGCGTTACGGAAACTGA
TCCACGACACCGCGCTGGTCGAAGGGATCGACCCCGACCGGCTCTCCTTCACCCACACCGTGACGATCGTCCGCCGCCAG
GTTGTGCGCCGGGCGATTTTCCCCCCTCCGCCGGACGGCCCGGATCCTGGCCGCGGTGACCACTGA

Upstream 100 bases:

>100_bases
GCCCGCCAGCGGGCCTGGGCGGCCTCGATGAGTTTGAACGCCATGGCCAGCCCGGCGGCCAATACCAGGAACTTAGCATC
TGATCGTGTAGGTTCGGTGG

Downstream 100 bases:

>100_bases
GATCGCCGAACACGTCCTCCCGAAACGCCTCCGCTCCAATCCCCGGGTCGTCCGGCGGACCAAGCGCACCCCGTTCCCCG
CCAAGAAGCCGATCGACAAG

Product: transposase IS4 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MPRRGQVKEKPEDRLVDRVGLGVLAAQFPDALVDRVVAETGRRERRTRDLPAALTLRYVVALALFPSDGYDEVMRQVKVA
DDWLSDKAGPVKVPATTAITKARDRLGVEPVKLLFERTAVPMALPRRTVGAFYRGWRVCTVDGTTLLVPDTDENAAAFGK
PGNDQGEGALPQVRVLGLVECGTRALLGAGFGGTGGSKAASEQALFPDLLGALRPGMLVLADRNFLGFELFAKAAATGAD
LLWRAKSDRRLPIDTELADGSYLSHLVEPGTRDKGRKITVRVVEYTLDRDPDSPLPAGKKETYRLVTTILDPDAAPATDL
AALYSDRWEVETLLDEIKVHQQDGRLVLRSRAPDRVEQEVWGVLLLHRALRKLIHDTALVEGIDPDRLSFTHTVTIVRRQ
VVRRAIFPPPPDGPDPGRGDH

Sequences:

>Translated_421_residues
MPRRGQVKEKPEDRLVDRVGLGVLAAQFPDALVDRVVAETGRRERRTRDLPAALTLRYVVALALFPSDGYDEVMRQVKVA
DDWLSDKAGPVKVPATTAITKARDRLGVEPVKLLFERTAVPMALPRRTVGAFYRGWRVCTVDGTTLLVPDTDENAAAFGK
PGNDQGEGALPQVRVLGLVECGTRALLGAGFGGTGGSKAASEQALFPDLLGALRPGMLVLADRNFLGFELFAKAAATGAD
LLWRAKSDRRLPIDTELADGSYLSHLVEPGTRDKGRKITVRVVEYTLDRDPDSPLPAGKKETYRLVTTILDPDAAPATDL
AALYSDRWEVETLLDEIKVHQQDGRLVLRSRAPDRVEQEVWGVLLLHRALRKLIHDTALVEGIDPDRLSFTHTVTIVRRQ
VVRRAIFPPPPDGPDPGRGDH
>Mature_420_residues
PRRGQVKEKPEDRLVDRVGLGVLAAQFPDALVDRVVAETGRRERRTRDLPAALTLRYVVALALFPSDGYDEVMRQVKVAD
DWLSDKAGPVKVPATTAITKARDRLGVEPVKLLFERTAVPMALPRRTVGAFYRGWRVCTVDGTTLLVPDTDENAAAFGKP
GNDQGEGALPQVRVLGLVECGTRALLGAGFGGTGGSKAASEQALFPDLLGALRPGMLVLADRNFLGFELFAKAAATGADL
LWRAKSDRRLPIDTELADGSYLSHLVEPGTRDKGRKITVRVVEYTLDRDPDSPLPAGKKETYRLVTTILDPDAAPATDLA
ALYSDRWEVETLLDEIKVHQQDGRLVLRSRAPDRVEQEVWGVLLLHRALRKLIHDTALVEGIDPDRLSFTHTVTIVRRQV
VRRAIFPPPPDGPDPGRGDH

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=395, Percent_Identity=27.8481012658228, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 46151; Mature: 46020

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRGQVKEKPEDRLVDRVGLGVLAAQFPDALVDRVVAETGRRERRTRDLPAALTLRYVV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHH
ALALFPSDGYDEVMRQVKVADDWLSDKAGPVKVPATTAITKARDRLGVEPVKLLFERTAV
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
PMALPRRTVGAFYRGWRVCTVDGTTLLVPDTDENAAAFGKPGNDQGEGALPQVRVLGLVE
CHHCCHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
CGTRALLGAGFGGTGGSKAASEQALFPDLLGALRPGMLVLADRNFLGFELFAKAAATGAD
HCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCH
LLWRAKSDRRLPIDTELADGSYLSHLVEPGTRDKGRKITVRVVEYTLDRDPDSPLPAGKK
HEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCH
ETYRLVTTILDPDAAPATDLAALYSDRWEVETLLDEIKVHQQDGRLVLRSRAPDRVEQEV
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
WGVLLLHRALRKLIHDTALVEGIDPDRLSFTHTVTIVRRQVVRRAIFPPPPDGPDPGRGD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
H
C
>Mature Secondary Structure 
PRRGQVKEKPEDRLVDRVGLGVLAAQFPDALVDRVVAETGRRERRTRDLPAALTLRYVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHH
ALALFPSDGYDEVMRQVKVADDWLSDKAGPVKVPATTAITKARDRLGVEPVKLLFERTAV
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHCC
PMALPRRTVGAFYRGWRVCTVDGTTLLVPDTDENAAAFGKPGNDQGEGALPQVRVLGLVE
CHHCCHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
CGTRALLGAGFGGTGGSKAASEQALFPDLLGALRPGMLVLADRNFLGFELFAKAAATGAD
HCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCH
LLWRAKSDRRLPIDTELADGSYLSHLVEPGTRDKGRKITVRVVEYTLDRDPDSPLPAGKK
HEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCH
ETYRLVTTILDPDAAPATDLAALYSDRWEVETLLDEIKVHQQDGRLVLRSRAPDRVEQEV
HHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
WGVLLLHRALRKLIHDTALVEGIDPDRLSFTHTVTIVRRQVVRRAIFPPPPDGPDPGRGD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]