Click here to switch to the map view.

The map label for this gene is curA [H]

Identifier: 158315158

GI number: 158315158

Start: 3976243

End: 3977577

Strand: Direct

Name: curA [H]

Synonym: Franean1_3358

Alternate gene names: 158315158

Gene position: 3976243-3977577 (Clockwise)

Preceding gene: 158315157

Following gene: 158315159

Centisome position: 44.27

GC content: 67.72

Gene sequence:

>1335_bases
GTGGACGAAGTGAGCGCACAGCCGGCTGACCGTGGCGATCGGGACGTCCTTGTCACCGGAATGGGCTTCTGCCTCCCCGG
CGAAGACGGTCCGGTCCTCACCGCCGAGGAGGTGTGGGACATCGCGTCGAACGGGCGTTCCTGCCTGGTCAAGGACGGTA
TTTACCACGGTCGGGTCGACCTGGCGCCGGAGATGTTCGAGGAGCGGCTTCCGGAGATTCCCAAGCTGTACTCGGCGCAG
CTCACGAAGTCGCACCGCTTCGGGCTTCTCTCCTTCGTGGAGGCCTGCTCGGACGCGAAGATCGATTTTCGGGCGGGCGC
CCTGCGTCACGCCGCGATACTCACCGGGCGAGGGGGGCTCGACGACAACGTCGACCCGTATCGGGCCCTGCACGAGGCGA
CACCCGAGACGCTTTCCGCGGACGCCGCGACCGCGTTGTTCATCCGGGCCCAGCTCGGCATCACCTCGTACGACGTCACG
CTCCTGCAGGCGTCGATCGCCCGGTCGATGGGCCCGAGCTTCACGGTTTCCTGTGGCTGTTCCTCCTCCACCATCCAGCT
CGGCAACGCCCTGCGGATGATCGCCGCCGGGGAGGTCGACATCGCGGTCGTCACCGGTGTCGACGCGTTCAGCATGGACC
TGATGCGCAACGCGCAGCGGCTTCTGCTCGCCGCGACGCGCGGCGGCCCGCAGCCCGGCGCGGACACCCTGCCCGAGACG
CTGCCGTCGATGGACCGGCCGATGCGCCCCTACGACGTCCGCGCCGACTGCGTGAACTTCGGCGAGGGCTCGGTCACCGT
GGTCCTGGAGAGCCGCCAGCACGCCGAGTGGCGCGGAGCACACGTCTACGGGCGCCTGATCGCGCAGGCCACCACACGTG
ACGGCCTTGCCAGCCCGCTGTCGCTGGACGACTCGGGCGCCTCACTGGTCGCCGCGGTGCGCAAGTGTCTCGGGAACCGC
TGGAGCATCGACCAGATTCCGTACATCAACGGTGGCAGCGACGGTGACATGAACGTCACGAACACCGAGGCGAACGCCGT
CCGGGAACTGTACGGGACGGCGGTCCCGTCGGTTCTTCTCAGCTCGCAGGAGGCGTGTTTCGGGCACAGCGGGGCCCAGT
CCGGAAACCTCGGCCTGGCGTTGACTCTCCTCATGATGGAGAAAAGCCTGGCGTGCCCCACCGCGAACTGTGAGCAGCCG
ATCGAGGGCCTGCCTTTCGATCCGATTCCGGGAACGCGGCCGCGGCCGTTGCGGTTCGATCACGCGCTGAGCTTCACCTA
CCAGATCGGCGGCCTGAAGAGCGTGATCCTCATGAGTTCCGCCGATGCTTCCTGA

Upstream 100 bases:

>100_bases
TCGCTTCGACCAGATAAAGAACACTATGACCGAAATCGTGACTTCTCCCTGATGGTGCGCGATCTTGTGTGTCAAGATGC
CGACCATGTTGGAGACCTGA

Downstream 100 bases:

>100_bases
CGATGGTTCCTGAATATCTGAAAACCTGAACACCTGCTGAGACCGGCCTGGGACCTCGGTCGGCCGTTTCGCGCGACCGG
GGCCGTGTGGCCGATTCGGC

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MDEVSAQPADRGDRDVLVTGMGFCLPGEDGPVLTAEEVWDIASNGRSCLVKDGIYHGRVDLAPEMFEERLPEIPKLYSAQ
LTKSHRFGLLSFVEACSDAKIDFRAGALRHAAILTGRGGLDDNVDPYRALHEATPETLSADAATALFIRAQLGITSYDVT
LLQASIARSMGPSFTVSCGCSSSTIQLGNALRMIAAGEVDIAVVTGVDAFSMDLMRNAQRLLLAATRGGPQPGADTLPET
LPSMDRPMRPYDVRADCVNFGEGSVTVVLESRQHAEWRGAHVYGRLIAQATTRDGLASPLSLDDSGASLVAAVRKCLGNR
WSIDQIPYINGGSDGDMNVTNTEANAVRELYGTAVPSVLLSSQEACFGHSGAQSGNLGLALTLLMMEKSLACPTANCEQP
IEGLPFDPIPGTRPRPLRFDHALSFTYQIGGLKSVILMSSADAS

Sequences:

>Translated_444_residues
MDEVSAQPADRGDRDVLVTGMGFCLPGEDGPVLTAEEVWDIASNGRSCLVKDGIYHGRVDLAPEMFEERLPEIPKLYSAQ
LTKSHRFGLLSFVEACSDAKIDFRAGALRHAAILTGRGGLDDNVDPYRALHEATPETLSADAATALFIRAQLGITSYDVT
LLQASIARSMGPSFTVSCGCSSSTIQLGNALRMIAAGEVDIAVVTGVDAFSMDLMRNAQRLLLAATRGGPQPGADTLPET
LPSMDRPMRPYDVRADCVNFGEGSVTVVLESRQHAEWRGAHVYGRLIAQATTRDGLASPLSLDDSGASLVAAVRKCLGNR
WSIDQIPYINGGSDGDMNVTNTEANAVRELYGTAVPSVLLSSQEACFGHSGAQSGNLGLALTLLMMEKSLACPTANCEQP
IEGLPFDPIPGTRPRPLRFDHALSFTYQIGGLKSVILMSSADAS
>Mature_444_residues
MDEVSAQPADRGDRDVLVTGMGFCLPGEDGPVLTAEEVWDIASNGRSCLVKDGIYHGRVDLAPEMFEERLPEIPKLYSAQ
LTKSHRFGLLSFVEACSDAKIDFRAGALRHAAILTGRGGLDDNVDPYRALHEATPETLSADAATALFIRAQLGITSYDVT
LLQASIARSMGPSFTVSCGCSSSTIQLGNALRMIAAGEVDIAVVTGVDAFSMDLMRNAQRLLLAATRGGPQPGADTLPET
LPSMDRPMRPYDVRADCVNFGEGSVTVVLESRQHAEWRGAHVYGRLIAQATTRDGLASPLSLDDSGASLVAAVRKCLGNR
WSIDQIPYINGGSDGDMNVTNTEANAVRELYGTAVPSVLLSSQEACFGHSGAQSGNLGLALTLLMMEKSLACPTANCEQP
IEGLPFDPIPGTRPRPLRFDHALSFTYQIGGLKSVILMSSADAS

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Has A Preference For Short Chain Acid Substrates And May Function To Supply The Octanoic Substrates For Lipoic Acid Biosynthe

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=283, Percent_Identity=23.321554770318, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1787337, Length=270, Percent_Identity=26.2962962962963, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788663, Length=272, Percent_Identity=24.6323529411765, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24644341, Length=231, Percent_Identity=24.6753246753247, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 47219; Mature: 47219

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEVSAQPADRGDRDVLVTGMGFCLPGEDGPVLTAEEVWDIASNGRSCLVKDGIYHGRVD
CCCCCCCCCCCCCCEEEEEECEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCC
LAPEMFEERLPEIPKLYSAQLTKSHRFGLLSFVEACSDAKIDFRAGALRHAAILTGRGGL
CCHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEHHCCHHEEEEEECCCCC
DDNVDPYRALHEATPETLSADAATALFIRAQLGITSYDVTLLQASIARSMGPSFTVSCGC
CCCCCHHHHHHHCCCCHHCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
SSSTIQLGNALRMIAAGEVDIAVVTGVDAFSMDLMRNAQRLLLAATRGGPQPGADTLPET
CCCCEECCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHH
LPSMDRPMRPYDVRADCVNFGEGSVTVVLESRQHAEWRGAHVYGRLIAQATTRDGLASPL
HHHCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SLDDSGASLVAAVRKCLGNRWSIDQIPYINGGSDGDMNVTNTEANAVRELYGTAVPSVLL
CCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
SSQEACFGHSGAQSGNLGLALTLLMMEKSLACPTANCEQPIEGLPFDPIPGTRPRPLRFD
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
HALSFTYQIGGLKSVILMSSADAS
HHEEEEEECCCCEEEEEEECCCCC
>Mature Secondary Structure
MDEVSAQPADRGDRDVLVTGMGFCLPGEDGPVLTAEEVWDIASNGRSCLVKDGIYHGRVD
CCCCCCCCCCCCCCEEEEEECEEEECCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCC
LAPEMFEERLPEIPKLYSAQLTKSHRFGLLSFVEACSDAKIDFRAGALRHAAILTGRGGL
CCHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEHHCCHHEEEEEECCCCC
DDNVDPYRALHEATPETLSADAATALFIRAQLGITSYDVTLLQASIARSMGPSFTVSCGC
CCCCCHHHHHHHCCCCHHCCCHHHEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECC
SSSTIQLGNALRMIAAGEVDIAVVTGVDAFSMDLMRNAQRLLLAATRGGPQPGADTLPET
CCCCEECCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHH
LPSMDRPMRPYDVRADCVNFGEGSVTVVLESRQHAEWRGAHVYGRLIAQATTRDGLASPL
HHHCCCCCCCCCCCHHHEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
SLDDSGASLVAAVRKCLGNRWSIDQIPYINGGSDGDMNVTNTEANAVRELYGTAVPSVLL
CCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
SSQEACFGHSGAQSGNLGLALTLLMMEKSLACPTANCEQPIEGLPFDPIPGTRPRPLRFD
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
HALSFTYQIGGLKSVILMSSADAS
HHEEEEEECCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644304 [H]