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The map label for this gene is lipA [H]

Identifier: 158315124

GI number: 158315124

Start: 3935620

End: 3936546

Strand: Direct

Name: lipA [H]

Synonym: Franean1_3323

Alternate gene names: 158315124

Gene position: 3935620-3936546 (Clockwise)

Preceding gene: 158315123

Following gene: 158315129

Centisome position: 43.82

GC content: 71.2

Gene sequence:

>927_bases
GTGACACCGGACGGCCGTGCGATGCTGCGGCTCGAGGTCCGCAACGCCCGGACCCCGGTCGAGAACAAGCCGCCGTGGAT
GCGCACCCGGATGCGCGTCGGTCCCGCCTACACCGAGGTGATGGCCGTGGTCCGCGAGTCCGGGCTGCACACGGTGTGCG
AGGAGGCCGCCTGCCCGAACATCTTCGAGTGCTGGGAGGATCGGGAGGCGACCTTCCTGATCGGCGGGGACGTCTGCACG
CGGCGTTGCGACTTCTGCCAGATCGACTCCGGCCGGCCCGCTCCGCTCGACCGGGACGAGCCTCGCCGGGTGGCCGAATC
CGTCGCCAGCCTGGGACTGCACTACGCCACCGTCACCGGGGTGGCACGGGACGACCTGGCCGACGGAGGGGCCTGGCTGT
ACGCCGAGACCGTGCGCCAGATCCATGCGCGTTCCTCCGGAACCGGCGTCGAGCTGTTGATCCCGGACTTCCGTGGTGAC
CCGGCCCTGCTGGCCGAGGTGTTCGGTGCCGGCCCGGAGGTGCTTGCCCACAATGTCGAGACGGTGCCCCGGATCTTCCG
CGGCATCCGGCCGGGCTTCCGCTACGAGCGCTCGCTTGAGGTGATCAACCGTGCCCACGCGGAAGGGATCGTCACGAAGT
CGAACCTGATCCTCGGTCTGGGTGAGACCGTCGACGAGGTCGACGCCGTGCTGGCGGACCTGCGGGCAGCTGGTTGTGAT
CTGGTGACGGTGACCCAGTACCTGCGGCCCGGCCCGCGCCACCATCCGGTGGAACGCTGGGTGGAGCCGGAGGAGTTCGA
ACGGTGGTCCGCCCGGGCCGCCGAGCTCGGCTTCGCCGGGGCGATGGCGGGGCCGCTGGTCCGCTCCTCCTACCGCGCGG
GCCGGCTGTATCGCCAGGCAGTGGCGCTCCGCGGTGAACGCCGCTGA

Upstream 100 bases:

>100_bases
AAGATTCTCTGGGAGAACGCGACAAAGCTGTACCGGATCGAGGAGCCGTCGGCGTCGTGGCGGGCCACCGCCGGGACGAG
CTGATGACCGAGGACGGCCC

Downstream 100 bases:

>100_bases
GCGCCGCCCGCCGCCCGCCGCCCGGGCTGACAGGCCTCCGGCCGGGCGGCGCGCGGCTGCGCCGGTCAGCTCGTGGCCGT
GTGCGAAGGACCAGTCCAGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTPDGRAMLRLEVRNARTPVENKPPWMRTRMRVGPAYTEVMAVVRESGLHTVCEEAACPNIFECWEDREATFLIGGDVCT
RRCDFCQIDSGRPAPLDRDEPRRVAESVASLGLHYATVTGVARDDLADGGAWLYAETVRQIHARSSGTGVELLIPDFRGD
PALLAEVFGAGPEVLAHNVETVPRIFRGIRPGFRYERSLEVINRAHAEGIVTKSNLILGLGETVDEVDAVLADLRAAGCD
LVTVTQYLRPGPRHHPVERWVEPEEFERWSARAAELGFAGAMAGPLVRSSYRAGRLYRQAVALRGERR

Sequences:

>Translated_308_residues
MTPDGRAMLRLEVRNARTPVENKPPWMRTRMRVGPAYTEVMAVVRESGLHTVCEEAACPNIFECWEDREATFLIGGDVCT
RRCDFCQIDSGRPAPLDRDEPRRVAESVASLGLHYATVTGVARDDLADGGAWLYAETVRQIHARSSGTGVELLIPDFRGD
PALLAEVFGAGPEVLAHNVETVPRIFRGIRPGFRYERSLEVINRAHAEGIVTKSNLILGLGETVDEVDAVLADLRAAGCD
LVTVTQYLRPGPRHHPVERWVEPEEFERWSARAAELGFAGAMAGPLVRSSYRAGRLYRQAVALRGERR
>Mature_307_residues
TPDGRAMLRLEVRNARTPVENKPPWMRTRMRVGPAYTEVMAVVRESGLHTVCEEAACPNIFECWEDREATFLIGGDVCTR
RCDFCQIDSGRPAPLDRDEPRRVAESVASLGLHYATVTGVARDDLADGGAWLYAETVRQIHARSSGTGVELLIPDFRGDP
ALLAEVFGAGPEVLAHNVETVPRIFRGIRPGFRYERSLEVINRAHAEGIVTKSNLILGLGETVDEVDAVLADLRAAGCDL
VTVTQYLRPGPRHHPVERWVEPEEFERWSARAAELGFAGAMAGPLVRSSYRAGRLYRQAVALRGERR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=294, Percent_Identity=42.5170068027211, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=42.2131147540984, Blast_Score=194, Evalue=6e-50,
Organism=Escherichia coli, GI1786846, Length=298, Percent_Identity=43.6241610738255, Blast_Score=251, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=40.5498281786942, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=287, Percent_Identity=43.9024390243902, Blast_Score=235, Evalue=8e-63,
Organism=Drosophila melanogaster, GI221513272, Length=294, Percent_Identity=44.8979591836735, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34128; Mature: 33997

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDGRAMLRLEVRNARTPVENKPPWMRTRMRVGPAYTEVMAVVRESGLHTVCEEAACPN
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCC
IFECWEDREATFLIGGDVCTRRCDFCQIDSGRPAPLDRDEPRRVAESVASLGLHYATVTG
HHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
VARDDLADGGAWLYAETVRQIHARSSGTGVELLIPDFRGDPALLAEVFGAGPEVLAHNVE
CHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHCHH
TVPRIFRGIRPGFRYERSLEVINRAHAEGIVTKSNLILGLGETVDEVDAVLADLRAAGCD
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCC
LVTVTQYLRPGPRHHPVERWVEPEEFERWSARAAELGFAGAMAGPLVRSSYRAGRLYRQA
HHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
VALRGERR
HHHCCCCC
>Mature Secondary Structure 
TPDGRAMLRLEVRNARTPVENKPPWMRTRMRVGPAYTEVMAVVRESGLHTVCEEAACPN
CCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCC
IFECWEDREATFLIGGDVCTRRCDFCQIDSGRPAPLDRDEPRRVAESVASLGLHYATVTG
HHHHHCCCCEEEEECCHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
VARDDLADGGAWLYAETVRQIHARSSGTGVELLIPDFRGDPALLAEVFGAGPEVLAHNVE
CHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHCHH
TVPRIFRGIRPGFRYERSLEVINRAHAEGIVTKSNLILGLGETVDEVDAVLADLRAAGCD
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEECCCEEEECCCCHHHHHHHHHHHHHCCCC
LVTVTQYLRPGPRHHPVERWVEPEEFERWSARAAELGFAGAMAGPLVRSSYRAGRLYRQA
HHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
VALRGERR
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA