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The map label for this gene is lcfB [H]

Identifier: 158314519

GI number: 158314519

Start: 3189088

End: 3190638

Strand: Direct

Name: lcfB [H]

Synonym: Franean1_2695

Alternate gene names: 158314519

Gene position: 3189088-3190638 (Clockwise)

Preceding gene: 158314518

Following gene: 158314520

Centisome position: 35.51

GC content: 66.15

Gene sequence:

>1551_bases
ATGTATCCGGGCTTCTACGCCGAGAAGGACCCCGCCAAGCCGGCCGTAGTGCTCTGCCCGGCCGGAGAGCGGGTCAGCTA
CGGATCGCTGGAGGCACGGTCCCGCCAGTTCGCCCGCGTGCTCCGAGCCCGCGGACTGCGGCCCGGCGACACGGTGGCCC
TCCTGGCCGAGAACCACGCGCGCTACCTGGAGGTGTACTGGGCAGCGATCCGCTCCGGGCTCTACCTGACGGCGGTCAAC
TGGCACCTGACCGCGGCCGAAGCCGCCCACCTGCTCGGTGACTCCGCAGCACGCGTACTCGTCACTACCGCCCGGTTCAC
CGACCTGGCCCGCACGGCCGCGGATCTCAGCCCGACCTGCTCAACGCTCCTCCTCCTGGACGGGACCGAGGACGGCTTCG
AATCGTACGAGGAAGTGATCGCGGCCCAGTCCGCCGCACCGCTCGCCGACCAGCCAGCCGGCGACGTCATGCTCTACTCC
TCCGGCACGACCGGACGCGCCAAAGGCATCCGACGCCCGCTGTCCGACCTGCAGGTGGACCAGCCCGGCCGCCCCAGTGC
CTCTCCGATGGCAAAGGCATTTCTCGGAATCGGCGAGGACTCGACATACCTAACCCCGGCGCCGCTGTACCACGCAGCTA
GCCTGCACTGGGCAGCCGGCGCCCACGAGCTCGGCGCGACACTCGTCATCATGGACCGCTTCGACGCCGAACAGATGCTT
GCCGTTATCGAAAAAGAACGAGTCACCCACGCCCAAGTCGTCCCCACGATGATGATCCGCCTACTGAAACTCCCGGCCGA
AGTACGAACGAGATACGACGTCTCCAGCCTCCGCTCATTGACACATGCGGGAGCACCCTGCCCCCCGGCCATCAAACGTC
AGATGATCGACTGGCTCGGCCCGATCGTCGACGAGTACTACTCCAGCACTGAAGGCTCCGGTATGACGTTCATCGGCTCC
GCCGACTGGCTGGCACATCCGGGATCTGTCGGCAGAACAATCATCGGCACCCCGCACATCTGCGACGACAACGGTAGGGA
GCTACCGGTAGGCGAGCCCGGGCTGCTGTACTTCGACCGGGGGACGGAGCACTTCGAATACCACAACGACCCCGAAAAGA
CTCGCGAGGGCCGCCACCCCAAGCACCCGACCTGGACGACCTCCGGAGACATGGGCTACGTCGATACCGACGGCTACCTA
TACCTGACGGACCGCAAAAGCTTCATGATCATATCCGGAGGGGTCAACATCTACCCCGCCGAGATCGAGGCCGCCCTCAT
CCTGCACCCCGCCATCACGGATGTCGCCGTCTTCGGCCTTCCGCACGCCGACATGGGCGAATATGTCCACGCCGTCGTTC
AGCCCACGGACGGCGTCGACGCCACACCCGAACTCGCCGAGCAAATCCGCGCGTTCGCCCGCGACCACCTCGCCGGCTAC
AAGGTCCCCCGAGCAATCACCTTCCGCGACCAGCTACCGCGCATGTCCACCGGCAAACTCGCCAAGAACGCCCTGCGCCA
GGAATACCTCGGTGCTGCGCTACCGCGGTAG

Upstream 100 bases:

>100_bases
GTGAAGGACCGGCTATACATCGGTCGTACCTGGGTCGCACCATCGGGAAGCAGTACCGCGGGGCAGCTGCCGTTGACCAC
ACACTCCTGGAGGAACGCGG

Downstream 100 bases:

>100_bases
CAGGAAAGGCGTTCAACAGCTACGGTGACACGGTGCCCGTGAAGCTGCTGGGATTTCTGCCGCCGCTGTTCCCCGTCTGC
GTTGAGTGACGCTGATCCTG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MYPGFYAEKDPAKPAVVLCPAGERVSYGSLEARSRQFARVLRARGLRPGDTVALLAENHARYLEVYWAAIRSGLYLTAVN
WHLTAAEAAHLLGDSAARVLVTTARFTDLARTAADLSPTCSTLLLLDGTEDGFESYEEVIAAQSAAPLADQPAGDVMLYS
SGTTGRAKGIRRPLSDLQVDQPGRPSASPMAKAFLGIGEDSTYLTPAPLYHAASLHWAAGAHELGATLVIMDRFDAEQML
AVIEKERVTHAQVVPTMMIRLLKLPAEVRTRYDVSSLRSLTHAGAPCPPAIKRQMIDWLGPIVDEYYSSTEGSGMTFIGS
ADWLAHPGSVGRTIIGTPHICDDNGRELPVGEPGLLYFDRGTEHFEYHNDPEKTREGRHPKHPTWTTSGDMGYVDTDGYL
YLTDRKSFMIISGGVNIYPAEIEAALILHPAITDVAVFGLPHADMGEYVHAVVQPTDGVDATPELAEQIRAFARDHLAGY
KVPRAITFRDQLPRMSTGKLAKNALRQEYLGAALPR

Sequences:

>Translated_516_residues
MYPGFYAEKDPAKPAVVLCPAGERVSYGSLEARSRQFARVLRARGLRPGDTVALLAENHARYLEVYWAAIRSGLYLTAVN
WHLTAAEAAHLLGDSAARVLVTTARFTDLARTAADLSPTCSTLLLLDGTEDGFESYEEVIAAQSAAPLADQPAGDVMLYS
SGTTGRAKGIRRPLSDLQVDQPGRPSASPMAKAFLGIGEDSTYLTPAPLYHAASLHWAAGAHELGATLVIMDRFDAEQML
AVIEKERVTHAQVVPTMMIRLLKLPAEVRTRYDVSSLRSLTHAGAPCPPAIKRQMIDWLGPIVDEYYSSTEGSGMTFIGS
ADWLAHPGSVGRTIIGTPHICDDNGRELPVGEPGLLYFDRGTEHFEYHNDPEKTREGRHPKHPTWTTSGDMGYVDTDGYL
YLTDRKSFMIISGGVNIYPAEIEAALILHPAITDVAVFGLPHADMGEYVHAVVQPTDGVDATPELAEQIRAFARDHLAGY
KVPRAITFRDQLPRMSTGKLAKNALRQEYLGAALPR
>Mature_516_residues
MYPGFYAEKDPAKPAVVLCPAGERVSYGSLEARSRQFARVLRARGLRPGDTVALLAENHARYLEVYWAAIRSGLYLTAVN
WHLTAAEAAHLLGDSAARVLVTTARFTDLARTAADLSPTCSTLLLLDGTEDGFESYEEVIAAQSAAPLADQPAGDVMLYS
SGTTGRAKGIRRPLSDLQVDQPGRPSASPMAKAFLGIGEDSTYLTPAPLYHAASLHWAAGAHELGATLVIMDRFDAEQML
AVIEKERVTHAQVVPTMMIRLLKLPAEVRTRYDVSSLRSLTHAGAPCPPAIKRQMIDWLGPIVDEYYSSTEGSGMTFIGS
ADWLAHPGSVGRTIIGTPHICDDNGRELPVGEPGLLYFDRGTEHFEYHNDPEKTREGRHPKHPTWTTSGDMGYVDTDGYL
YLTDRKSFMIISGGVNIYPAEIEAALILHPAITDVAVFGLPHADMGEYVHAVVQPTDGVDATPELAEQIRAFARDHLAGY
KVPRAITFRDQLPRMSTGKLAKNALRQEYLGAALPR

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=540, Percent_Identity=26.6666666666667, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI38505220, Length=520, Percent_Identity=26.3461538461538, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI122937307, Length=523, Percent_Identity=27.5334608030593, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI42544132, Length=541, Percent_Identity=26.2476894639556, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=27.3743016759777, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=27.3743016759777, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI8923896, Length=541, Percent_Identity=24.5841035120148, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=24.7081712062257, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI62865631, Length=544, Percent_Identity=24.4485294117647, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI13325055, Length=544, Percent_Identity=24.4485294117647, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=26.3888888888889, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=25.5952380952381, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=25.5952380952381, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI253970489, Length=363, Percent_Identity=26.9972451790634, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI227499619, Length=552, Percent_Identity=23.731884057971, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI38524616, Length=544, Percent_Identity=24.8161764705882, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI28416953, Length=533, Percent_Identity=23.6397748592871, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI13375727, Length=535, Percent_Identity=23.7383177570093, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=538, Percent_Identity=28.8104089219331, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=27.4161735700197, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=27.027027027027, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790505, Length=526, Percent_Identity=23.384030418251, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=504, Percent_Identity=25.7936507936508, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786529, Length=566, Percent_Identity=24.0282685512367, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=26.5267175572519, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=25.7309941520468, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=543, Percent_Identity=25.414364640884, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=543, Percent_Identity=25.414364640884, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=543, Percent_Identity=25.414364640884, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=26.6666666666667, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=507, Percent_Identity=27.2189349112426, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=515, Percent_Identity=25.2427184466019, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=505, Percent_Identity=26.1386138613861, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI115534440, Length=554, Percent_Identity=22.5631768953069, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=537, Percent_Identity=24.2085661080074, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=537, Percent_Identity=24.2085661080074, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=386, Percent_Identity=24.6113989637306, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=487, Percent_Identity=24.435318275154, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=565, Percent_Identity=24.070796460177, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=564, Percent_Identity=22.3404255319149, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=27.8118609406953, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24581924, Length=545, Percent_Identity=26.2385321100917, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922652, Length=500, Percent_Identity=24.4, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356947, Length=523, Percent_Identity=25.4302103250478, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=25.7367387033399, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=24.4575936883629, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=24.3809523809524, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653035, Length=530, Percent_Identity=23.5849056603774, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=22.962962962963, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356441, Length=502, Percent_Identity=24.1035856573705, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667955, Length=540, Percent_Identity=22.962962962963, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667959, Length=371, Percent_Identity=24.5283018867925, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648255, Length=530, Percent_Identity=24.5283018867925, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648253, Length=530, Percent_Identity=24.3396226415094, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20130357, Length=507, Percent_Identity=24.8520710059172, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45552801, Length=556, Percent_Identity=23.7410071942446, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550496, Length=556, Percent_Identity=23.7410071942446, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21358303, Length=475, Percent_Identity=23.1578947368421, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281365686, Length=493, Percent_Identity=22.5152129817444, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161076582, Length=540, Percent_Identity=22.2222222222222, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648257, Length=489, Percent_Identity=24.9488752556237, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355225, Length=392, Percent_Identity=26.7857142857143, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24583465, Length=542, Percent_Identity=23.9852398523985, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583463, Length=542, Percent_Identity=23.9852398523985, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281364798, Length=542, Percent_Identity=23.9852398523985, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56203; Mature: 56203

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPGFYAEKDPAKPAVVLCPAGERVSYGSLEARSRQFARVLRARGLRPGDTVALLAENHA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
RYLEVYWAAIRSGLYLTAVNWHLTAAEAAHLLGDSAARVLVTTARFTDLARTAADLSPTC
HHHHHHHHHHHCCEEEEEEEEEEEHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCC
STLLLLDGTEDGFESYEEVIAAQSAAPLADQPAGDVMLYSSGTTGRAKGIRRPLSDLQVD
EEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCHHHCCCC
QPGRPSASPMAKAFLGIGEDSTYLTPAPLYHAASLHWAAGAHELGATLVIMDRFDAEQML
CCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHCCCHHCCCCHHHCCEEEEEECCCHHHHH
AVIEKERVTHAQVVPTMMIRLLKLPAEVRTRYDVSSLRSLTHAGAPCPPAIKRQMIDWLG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
PIVDEYYSSTEGSGMTFIGSADWLAHPGSVGRTIIGTPHICDDNGRELPVGEPGLLYFDR
HHHHHHHCCCCCCCEEEEECCCHHCCCCCCCCEEECCCCEECCCCCCCCCCCCCEEEECC
GTEHFEYHNDPEKTREGRHPKHPTWTTSGDMGYVDTDGYLYLTDRKSFMIISGGVNIYPA
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCCEEEEC
EIEAALILHPAITDVAVFGLPHADMGEYVHAVVQPTDGVDATPELAEQIRAFARDHLAGY
CCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
KVPRAITFRDQLPRMSTGKLAKNALRQEYLGAALPR
CCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYPGFYAEKDPAKPAVVLCPAGERVSYGSLEARSRQFARVLRARGLRPGDTVALLAENHA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCH
RYLEVYWAAIRSGLYLTAVNWHLTAAEAAHLLGDSAARVLVTTARFTDLARTAADLSPTC
HHHHHHHHHHHCCEEEEEEEEEEEHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCC
STLLLLDGTEDGFESYEEVIAAQSAAPLADQPAGDVMLYSSGTTGRAKGIRRPLSDLQVD
EEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHCCHHHCCCC
QPGRPSASPMAKAFLGIGEDSTYLTPAPLYHAASLHWAAGAHELGATLVIMDRFDAEQML
CCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHCCCHHCCCCHHHCCEEEEEECCCHHHHH
AVIEKERVTHAQVVPTMMIRLLKLPAEVRTRYDVSSLRSLTHAGAPCPPAIKRQMIDWLG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
PIVDEYYSSTEGSGMTFIGSADWLAHPGSVGRTIIGTPHICDDNGRELPVGEPGLLYFDR
HHHHHHHCCCCCCCEEEEECCCHHCCCCCCCCEEECCCCEECCCCCCCCCCCCCEEEECC
GTEHFEYHNDPEKTREGRHPKHPTWTTSGDMGYVDTDGYLYLTDRKSFMIISGGVNIYPA
CCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEECCCEEEEECCCEEEEEECCCEEEEC
EIEAALILHPAITDVAVFGLPHADMGEYVHAVVQPTDGVDATPELAEQIRAFARDHLAGY
CCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
KVPRAITFRDQLPRMSTGKLAKNALRQEYLGAALPR
CCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]