Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 158314517

GI number: 158314517

Start: 3186476

End: 3187417

Strand: Direct

Name: paaF [C]

Synonym: Franean1_2693

Alternate gene names: 158314517

Gene position: 3186476-3187417 (Clockwise)

Preceding gene: 158314516

Following gene: 158314518

Centisome position: 35.48

GC content: 72.93

Gene sequence:

>942_bases
GTGACCGCGGGGCAGCCGGCGCCGAGCACATCGGCGCGAACCACCACCCCCATGGCAACCCCGTCCTCGGGCGGGTCGCC
GTGGGCCGAGGACGGCACCATTGAGGTCGAGATCGCCGATCGAGTCGCCACGGTCACGCTGACCCGACCACCCGGCAACG
CCTGGACCCCCCGGCTGGGCCGGCAGTTCTTCGCCGCCCTCGACCGGCTGGCCCTCGACCCCGTAGTTCGCGCCGTGGTG
GTGACCGGCGCCGGCCCGGACTTCTGCGTCGGCGCCGACGGTGGCAGCCTCGACAACGTCGCGGACGCAGGCTCCTACGA
GATCGACGCGGCCCGGCCGACGTACTGGGCCGCGATGACGATCGCCAAACCGGTCGTGGCGGCGGTCAACGGCGCCTGTT
TCGGCGTCGGGATGCAGCTGGCGCTGTTCTGCGACGTGCGGTTCGCCGGCTCGTCGACGAAGTTCGCCACCGCGTTCGTC
CGCCGCGGCCTGATCGCCGAGCTCGGCATGACCTGGCTGCTGCCCCGGGTCGTCGGGCTCGGCGTCGCCAACGACCTGCT
GCTGTCCGGCCGCCTGGTCCGCGCCGCCGAGGCCGAACGCCTCGGCCTGGCCAACAGGATCGTCGACGACGGCGAACTGC
TGGCCGCCGCACAGGACTACGCGGCGGGACTGGCCGTTGGCTGCTCACCGGCCGCGATGGGCGTCGTCAAACGCCAGACC
TACACGGACCTGACCTCCACGCTGCCCGCCGCGGTCGCCCGCGCCGACACCTACCTGGAGGCGGCGCTGGCGTCGGCGGA
CTTCGCCGAGGGTGTGCGCAGCTGGCGCGAGCGACGCCCACCAGCCTTCGCCGGGCTCGACCCCACCCATGCTCGGTTCA
CTCTCCCAGACGCCGTCGAGCCGTCAGCCGGCGAGACCAGACAGGGGGAACGACAAGGATGA

Upstream 100 bases:

>100_bases
GCCTGGCCGAACCGCTCCAGTTCGACAAGGAGATCGAGTGCTTCGACGCCACCACCAAGGCTCGGATCATGCGGGGCAAC
TCCGCCGCGCTCCTCGGGCT

Downstream 100 bases:

>100_bases
CCGGACCACACGACGTACAGGTGATCGAGACGCTGATGGGTTTCCGGTCGAGGGCCTTGGTCATCGACAACATTCCGAAC
CGTCGCGCGTCAGCTCTTGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTAGQPAPSTSARTTTPMATPSSGGSPWAEDGTIEVEIADRVATVTLTRPPGNAWTPRLGRQFFAALDRLALDPVVRAVV
VTGAGPDFCVGADGGSLDNVADAGSYEIDAARPTYWAAMTIAKPVVAAVNGACFGVGMQLALFCDVRFAGSSTKFATAFV
RRGLIAELGMTWLLPRVVGLGVANDLLLSGRLVRAAEAERLGLANRIVDDGELLAAAQDYAAGLAVGCSPAAMGVVKRQT
YTDLTSTLPAAVARADTYLEAALASADFAEGVRSWRERRPPAFAGLDPTHARFTLPDAVEPSAGETRQGERQG

Sequences:

>Translated_313_residues
MTAGQPAPSTSARTTTPMATPSSGGSPWAEDGTIEVEIADRVATVTLTRPPGNAWTPRLGRQFFAALDRLALDPVVRAVV
VTGAGPDFCVGADGGSLDNVADAGSYEIDAARPTYWAAMTIAKPVVAAVNGACFGVGMQLALFCDVRFAGSSTKFATAFV
RRGLIAELGMTWLLPRVVGLGVANDLLLSGRLVRAAEAERLGLANRIVDDGELLAAAQDYAAGLAVGCSPAAMGVVKRQT
YTDLTSTLPAAVARADTYLEAALASADFAEGVRSWRERRPPAFAGLDPTHARFTLPDAVEPSAGETRQGERQG
>Mature_312_residues
TAGQPAPSTSARTTTPMATPSSGGSPWAEDGTIEVEIADRVATVTLTRPPGNAWTPRLGRQFFAALDRLALDPVVRAVVV
TGAGPDFCVGADGGSLDNVADAGSYEIDAARPTYWAAMTIAKPVVAAVNGACFGVGMQLALFCDVRFAGSSTKFATAFVR
RGLIAELGMTWLLPRVVGLGVANDLLLSGRLVRAAEAERLGLANRIVDDGELLAAAQDYAAGLAVGCSPAAMGVVKRQTY
TDLTSTLPAAVARADTYLEAALASADFAEGVRSWRERRPPAFAGLDPTHARFTLPDAVEPSAGETRQGERQG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=265, Percent_Identity=29.811320754717, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI70995211, Length=301, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68989263, Length=182, Percent_Identity=33.5164835164835, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=241, Percent_Identity=29.045643153527, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI20127408, Length=195, Percent_Identity=28.7179487179487, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI221136756, Length=237, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136753, Length=237, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221307494, Length=237, Percent_Identity=25.3164556962025, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=31.3253012048193, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=35.0597609561753, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=30.46875, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=31.5789473684211, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=29.5081967213115, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=192, Percent_Identity=34.375, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=224, Percent_Identity=31.6964285714286, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=174, Percent_Identity=29.3103448275862, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=264, Percent_Identity=26.1363636363636, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=194, Percent_Identity=30.4123711340206, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=259, Percent_Identity=26.6409266409266, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=183, Percent_Identity=30.0546448087432, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=190, Percent_Identity=27.3684210526316, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20129971, Length=269, Percent_Identity=31.2267657992565, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=269, Percent_Identity=31.2267657992565, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=28.5185185185185, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=261, Percent_Identity=29.1187739463602, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357171, Length=295, Percent_Identity=28.8135593220339, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=30.1075268817204, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583077, Length=185, Percent_Identity=31.8918918918919, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583079, Length=185, Percent_Identity=31.8918918918919, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=28.804347826087, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650670, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 32521; Mature: 32390

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGQPAPSTSARTTTPMATPSSGGSPWAEDGTIEVEIADRVATVTLTRPPGNAWTPRLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCHHHH
RQFFAALDRLALDPVVRAVVVTGAGPDFCVGADGGSLDNVADAGSYEIDAARPTYWAAMT
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHH
IAKPVVAAVNGACFGVGMQLALFCDVRFAGSSTKFATAFVRRGLIAELGMTWLLPRVVGL
HHHHHHHHHCCHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVANDLLLSGRLVRAAEAERLGLANRIVDDGELLAAAQDYAAGLAVGCSPAAMGVVKRQT
CCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHH
YTDLTSTLPAAVARADTYLEAALASADFAEGVRSWRERRPPAFAGLDPTHARFTLPDAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
PSAGETRQGERQG
CCCCCCCCCCCCC
>Mature Secondary Structure 
TAGQPAPSTSARTTTPMATPSSGGSPWAEDGTIEVEIADRVATVTLTRPPGNAWTPRLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCHHHH
RQFFAALDRLALDPVVRAVVVTGAGPDFCVGADGGSLDNVADAGSYEIDAARPTYWAAMT
HHHHHHHHHHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHH
IAKPVVAAVNGACFGVGMQLALFCDVRFAGSSTKFATAFVRRGLIAELGMTWLLPRVVGL
HHHHHHHHHCCHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVANDLLLSGRLVRAAEAERLGLANRIVDDGELLAAAQDYAAGLAVGCSPAAMGVVKRQT
CCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHH
YTDLTSTLPAAVARADTYLEAALASADFAEGVRSWRERRPPAFAGLDPTHARFTLPDAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCC
PSAGETRQGERQG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA