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The map label for this gene is tcmA [H]

Identifier: 158314351

GI number: 158314351

Start: 3000770

End: 3002263

Strand: Direct

Name: tcmA [H]

Synonym: Franean1_2521

Alternate gene names: 158314351

Gene position: 3000770-3002263 (Clockwise)

Preceding gene: 158314350

Following gene: 158314352

Centisome position: 33.41

GC content: 65.6

Gene sequence:

>1494_bases
ATGGAGCGCGAAGACTCCGGACGCGTCGACGCGCGGAAGACGGATGCAGGGCCGCGGCGCTGGTGGATCCTGGGAGCTCT
CTGCCTCGGTCTGGTCGTCGTCGAGGTCGACGCGAGCATTCTCAACGTCGCGATCCCGTCCATCGCGGCTGACCTTGACG
CCGATCCGGCCGACATGGCGTGGATCGTCGACTCCTTCGTCCTCTCCTTCGCAAGCTTCATGCTCGTCGCCGGACGCCTG
GGTGACCGTTTTGGGCACAGGAAGATACTCGAGGCGGGACTCCTGCTGTTCTCCCTGGCCTCCTTCGCCGCCACCTTCGC
GGACTCACCCGCGGGCCTGGTCGCATGTCGCGCGGTCCTCGGTATCGGCGCCGCGGCTATCCTGCCGACATCGAGAGCGC
TGGTCATGGCGGCGTTCCCCTCCGCCGAGCACCCCAGAGCCCTGGGGTACTGGACCGCGGCAATAGGAGTGAGCTTACCG
CTGGGACCTTTGCTCGGCGGGCTGCTCCTCGACCATTTCTGGTGGGGTTCCGTCTTCCTCGTGGGCGGGCCCACCTCGCT
GCTGGCCGGTGTCATCAACGCGGTGGTGCTCGGGCGTCAGAGGGCTGGTGGGGGCAAAGGCGGATGGGATCCGCCTGGGA
TTGTCCTGTCCGCGTTCGGAACAGCAGCATTGATATTCGGCATCATCGAGGGCCCTCGCCTGGGGTGGCTCTCCGCCACG
ATTCTGGCATGCCATGGCTCCGCCCTGGTGGCACTGGCCGCCTTCTTCGTCTGGGAACGTCATGCGACGCACCCGGTGGT
CGAACCGGAACTGCTGCGAGTGCGATCGTTCACCGTGGGCTCGATGGTCGCCGCACTCTCGCTGTTCGTGTTCAACGGGC
TCCTCTTCGTGCTCACCCAGTATCTCCAGATCCTCGAGGGATACAGCCCTCTCCAGTCCGGTCTCCGAGTGATCCCGCTG
GGAGCGGGATTCATGCTTGGCAGCGTCTTGTCACGCCGTGCGGCCCTGCGAATCGGTCAACGTGGAACGCTGGCCATCGG
GTTCGCGGCGGTGGGATCGGCCCTCCTGGTACTCCTGCTCGGTACCTGGTCCGACGGTTATCTCGTCACGGGTGTCGGTG
TTCTGATCGTCGGCTGGGGAACCGGGCTGACAATGGCCTGCGCCGTCCATCTGGCACTGAGCGAGGTTCCGGCGTCCACA
GCGGGCGCCGCCGGAGCCTTCAGTAACTCGGTAAGGCAGTTGGGGGCGGCACTCGGCGTCGCAGTCCTCGGCGCCGCACT
CGGGACTTCCGCAGGTAGAAGTGGCGCGCAGAACCCTTCTTCCTCTGATCCTGTCAGCAATACCGCTGGAAGCGTCGTGG
CGGACATTGCGACCGCTCGTCCGGGCCTTGGCATGGAAAACGGGTTCAGGCTCGCCTTCGCCCTGGCGGCGATCGTGAGT
TGCCTGTGCATCGTGCTTGTCGCCGTGGCTTTCAGGAAAGTTCGATCAGCCTGA

Upstream 100 bases:

>100_bases
TGCTACACCCGGTCGATGCCCCACTTCGGACCTGGCGGGTCGGGGGAAAGCCCTCGGCCCGCCCTCCGGACTGACCTGCA
TGCGTGGTGGCCAGCCAGCG

Downstream 100 bases:

>100_bases
CGGGGGAGCGGCGTGTTCGACGTCGATGCCACGCTCGGCGAGCAGCGCCTCGACGTCCCGGCTGGGCTGTGGGGTGCCAG
AAGTCGGCCTGTGCCCGTGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MEREDSGRVDARKTDAGPRRWWILGALCLGLVVVEVDASILNVAIPSIAADLDADPADMAWIVDSFVLSFASFMLVAGRL
GDRFGHRKILEAGLLLFSLASFAATFADSPAGLVACRAVLGIGAAAILPTSRALVMAAFPSAEHPRALGYWTAAIGVSLP
LGPLLGGLLLDHFWWGSVFLVGGPTSLLAGVINAVVLGRQRAGGGKGGWDPPGIVLSAFGTAALIFGIIEGPRLGWLSAT
ILACHGSALVALAAFFVWERHATHPVVEPELLRVRSFTVGSMVAALSLFVFNGLLFVLTQYLQILEGYSPLQSGLRVIPL
GAGFMLGSVLSRRAALRIGQRGTLAIGFAAVGSALLVLLLGTWSDGYLVTGVGVLIVGWGTGLTMACAVHLALSEVPAST
AGAAGAFSNSVRQLGAALGVAVLGAALGTSAGRSGAQNPSSSDPVSNTAGSVVADIATARPGLGMENGFRLAFALAAIVS
CLCIVLVAVAFRKVRSA

Sequences:

>Translated_497_residues
MEREDSGRVDARKTDAGPRRWWILGALCLGLVVVEVDASILNVAIPSIAADLDADPADMAWIVDSFVLSFASFMLVAGRL
GDRFGHRKILEAGLLLFSLASFAATFADSPAGLVACRAVLGIGAAAILPTSRALVMAAFPSAEHPRALGYWTAAIGVSLP
LGPLLGGLLLDHFWWGSVFLVGGPTSLLAGVINAVVLGRQRAGGGKGGWDPPGIVLSAFGTAALIFGIIEGPRLGWLSAT
ILACHGSALVALAAFFVWERHATHPVVEPELLRVRSFTVGSMVAALSLFVFNGLLFVLTQYLQILEGYSPLQSGLRVIPL
GAGFMLGSVLSRRAALRIGQRGTLAIGFAAVGSALLVLLLGTWSDGYLVTGVGVLIVGWGTGLTMACAVHLALSEVPAST
AGAAGAFSNSVRQLGAALGVAVLGAALGTSAGRSGAQNPSSSDPVSNTAGSVVADIATARPGLGMENGFRLAFALAAIVS
CLCIVLVAVAFRKVRSA
>Mature_497_residues
MEREDSGRVDARKTDAGPRRWWILGALCLGLVVVEVDASILNVAIPSIAADLDADPADMAWIVDSFVLSFASFMLVAGRL
GDRFGHRKILEAGLLLFSLASFAATFADSPAGLVACRAVLGIGAAAILPTSRALVMAAFPSAEHPRALGYWTAAIGVSLP
LGPLLGGLLLDHFWWGSVFLVGGPTSLLAGVINAVVLGRQRAGGGKGGWDPPGIVLSAFGTAALIFGIIEGPRLGWLSAT
ILACHGSALVALAAFFVWERHATHPVVEPELLRVRSFTVGSMVAALSLFVFNGLLFVLTQYLQILEGYSPLQSGLRVIPL
GAGFMLGSVLSRRAALRIGQRGTLAIGFAAVGSALLVLLLGTWSDGYLVTGVGVLIVGWGTGLTMACAVHLALSEVPAST
AGAAGAFSNSVRQLGAALGVAVLGAALGTSAGRSGAQNPSSSDPVSNTAGSVVADIATARPGLGMENGFRLAFALAAIVS
CLCIVLVAVAFRKVRSA

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=450, Percent_Identity=26, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788710, Length=444, Percent_Identity=24.7747747747748, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=27.710843373494, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=28.2973621103118, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=26.8408551068884, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI48994889, Length=136, Percent_Identity=33.0882352941176, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=303, Percent_Identity=27.3927392739274, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=395, Percent_Identity=23.0379746835443, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50898; Mature: 50898

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEREDSGRVDARKTDAGPRRWWILGALCLGLVVVEVDASILNVAIPSIAADLDADPADMA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
WIVDSFVLSFASFMLVAGRLGDRFGHRKILEAGLLLFSLASFAATFADSPAGLVACRAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GIGAAAILPTSRALVMAAFPSAEHPRALGYWTAAIGVSLPLGPLLGGLLLDHFWWGSVFL
HCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
VGGPTSLLAGVINAVVLGRQRAGGGKGGWDPPGIVLSAFGTAALIFGIIEGPRLGWLSAT
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
ILACHGSALVALAAFFVWERHATHPVVEPELLRVRSFTVGSMVAALSLFVFNGLLFVLTQ
HHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQILEGYSPLQSGLRVIPLGAGFMLGSVLSRRAALRIGQRGTLAIGFAAVGSALLVLLL
HHHHHCCCCHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
GTWSDGYLVTGVGVLIVGWGTGLTMACAVHLALSEVPASTAGAAGAFSNSVRQLGAALGV
HCCCCCEEEEECEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AVLGAALGTSAGRSGAQNPSSSDPVSNTAGSVVADIATARPGLGMENGFRLAFALAAIVS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
CLCIVLVAVAFRKVRSA
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEREDSGRVDARKTDAGPRRWWILGALCLGLVVVEVDASILNVAIPSIAADLDADPADMA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
WIVDSFVLSFASFMLVAGRLGDRFGHRKILEAGLLLFSLASFAATFADSPAGLVACRAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GIGAAAILPTSRALVMAAFPSAEHPRALGYWTAAIGVSLPLGPLLGGLLLDHFWWGSVFL
HCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEE
VGGPTSLLAGVINAVVLGRQRAGGGKGGWDPPGIVLSAFGTAALIFGIIEGPRLGWLSAT
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
ILACHGSALVALAAFFVWERHATHPVVEPELLRVRSFTVGSMVAALSLFVFNGLLFVLTQ
HHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQILEGYSPLQSGLRVIPLGAGFMLGSVLSRRAALRIGQRGTLAIGFAAVGSALLVLLL
HHHHHCCCCHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
GTWSDGYLVTGVGVLIVGWGTGLTMACAVHLALSEVPASTAGAAGAFSNSVRQLGAALGV
HCCCCCEEEEECEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
AVLGAALGTSAGRSGAQNPSSSDPVSNTAGSVVADIATARPGLGMENGFRLAFALAAIVS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
CLCIVLVAVAFRKVRSA
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]