Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 158314241

GI number: 158314241

Start: 2865822

End: 2867066

Strand: Direct

Name: paaF [C]

Synonym: Franean1_2411

Alternate gene names: 158314241

Gene position: 2865822-2867066 (Clockwise)

Preceding gene: 158314237

Following gene: 158314242

Centisome position: 31.91

GC content: 76.63

Gene sequence:

>1245_bases
GTGGGCGCCGTGGATGTTCGTCAGGACCAGGCGGGGGCCCGGCCGACCGGGCGGGGGAGTACGACGATCGTCCCCGGCGT
CGCTGCCCCGAATGTCGCCGCCGCGAATGTGGCGGATGTGGCTGTCCTGGAGGTCGATGTCCTGGAGCCGGAGGAGGCGC
TGCTCCTGCTGCGCGCCGACCCCGAGATGTTCGCCGCGCACACCGGACGGCCGCTGCTGGCCGTGCGCGCCCACGGCCCG
CTGGCGGTCGAGGTCGGTGAGCTCGCCCGCCGGCTGCCGTGCGTGCTGGTGGGTGTCGCGCCGGATCTCGCCGATCTCCC
CTCGGGCATCGAGTCGGCCGGCTACGACGTGCTGCTGACCGGCGAGGCCACGCCGCCCGCCCCGTGGGTCGGCCCGCTGC
GGCCGGGCGACGGGTTCACGGCCGCCCTCGGCGCGATCGCAGCCATGACCCGTGCCGTCCCCGGCGCCTGCGTGGCGTTC
ACCCAGCTGGTGCGGTACTCGGCGGGGATCGACGTCCGCGACGCCGTGATCGCCGAGTCGTTCACCTACTCGACGCTGCA
AGGGGGCCCCGAGTTCGCCGCCTGGCTCGCCGAGCACCGTGCCGTGCGGGAGCGTCGCGAGGCCGGGGCCGCCACCGCGC
CGGTGCGCGCCGAGCCCACCGGCCAGCCCACGGAGCCGGCGGTTCTCGTCGAGCGGGACGGCGACGAGCTGTGGGTCGTG
CTGAACCGGCCGCGCGTGCACAACGCGGTGAGCCGCGATCTGCGGGACGGCCTCGCCGCCGCGTTCGCCCTGGCCGGGGC
CGACCCGACCGTCGCCGAGGTGCGGCTGCGGGGGGCCGGCCGCTCGTTCTGCAGTGGCGGCGACCTCACCGAGTTCGGCT
CGTTGCCCGACCCGGCCCGCGCCCACGCGGTGCGGACCACCCGCAGCATCCCGCTGTCACTGCTGCGCTGCGCGCGCCGG
GTCACCGCGTACATGCACGGGACCTGCGTGGGAGCCGGGGTGGAGGTCCCGGCCTTCGCCCGGAGGGTGATCGCCGATCC
GGCGACCACCCTCCGGCTGCCCGAGCTCGCGATGGGGCTGGTGCCCGGCGCGGGTGGGACGTCCAGCGTCGTGCGCCGGG
TCGGCCGGGAGCGCGCCGCGTTCCTGTCGCTGACCGGCTGGCCGCTGGACGCCGCCACCGCGGCGAACTGGGGCCTGGTT
GACGAGGTCCGCCCAGTGCACGGAGACGCCCGCCCCCCGGTGTGA

Upstream 100 bases:

>100_bases
TCTGACCAGCACGGGATCACCTCGGGGCGCGGGGGAGCGGCAGCCCGAGAGTACGGTGTGCGGGCCTGTGCCTGGCACGA
CGGCACGGGACGGGCCTAAC

Downstream 100 bases:

>100_bases
TCGCCCAGGCCGCGGCGCGGCGAGTTGGGTGACGACCTCCCGTCCCGGGTTAACTCCGCCCCTGTCGGGCAGAGATGGGG
GCAGGCCTGACCCCAACGAA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGAVDVRQDQAGARPTGRGSTTIVPGVAAPNVAAANVADVAVLEVDVLEPEEALLLLRADPEMFAAHTGRPLLAVRAHGP
LAVEVGELARRLPCVLVGVAPDLADLPSGIESAGYDVLLTGEATPPAPWVGPLRPGDGFTAALGAIAAMTRAVPGACVAF
TQLVRYSAGIDVRDAVIAESFTYSTLQGGPEFAAWLAEHRAVRERREAGAATAPVRAEPTGQPTEPAVLVERDGDELWVV
LNRPRVHNAVSRDLRDGLAAAFALAGADPTVAEVRLRGAGRSFCSGGDLTEFGSLPDPARAHAVRTTRSIPLSLLRCARR
VTAYMHGTCVGAGVEVPAFARRVIADPATTLRLPELAMGLVPGAGGTSSVVRRVGRERAAFLSLTGWPLDAATAANWGLV
DEVRPVHGDARPPV

Sequences:

>Translated_414_residues
MGAVDVRQDQAGARPTGRGSTTIVPGVAAPNVAAANVADVAVLEVDVLEPEEALLLLRADPEMFAAHTGRPLLAVRAHGP
LAVEVGELARRLPCVLVGVAPDLADLPSGIESAGYDVLLTGEATPPAPWVGPLRPGDGFTAALGAIAAMTRAVPGACVAF
TQLVRYSAGIDVRDAVIAESFTYSTLQGGPEFAAWLAEHRAVRERREAGAATAPVRAEPTGQPTEPAVLVERDGDELWVV
LNRPRVHNAVSRDLRDGLAAAFALAGADPTVAEVRLRGAGRSFCSGGDLTEFGSLPDPARAHAVRTTRSIPLSLLRCARR
VTAYMHGTCVGAGVEVPAFARRVIADPATTLRLPELAMGLVPGAGGTSSVVRRVGRERAAFLSLTGWPLDAATAANWGLV
DEVRPVHGDARPPV
>Mature_413_residues
GAVDVRQDQAGARPTGRGSTTIVPGVAAPNVAAANVADVAVLEVDVLEPEEALLLLRADPEMFAAHTGRPLLAVRAHGPL
AVEVGELARRLPCVLVGVAPDLADLPSGIESAGYDVLLTGEATPPAPWVGPLRPGDGFTAALGAIAAMTRAVPGACVAFT
QLVRYSAGIDVRDAVIAESFTYSTLQGGPEFAAWLAEHRAVRERREAGAATAPVRAEPTGQPTEPAVLVERDGDELWVVL
NRPRVHNAVSRDLRDGLAAAFALAGADPTVAEVRLRGAGRSFCSGGDLTEFGSLPDPARAHAVRTTRSIPLSLLRCARRV
TAYMHGTCVGAGVEVPAFARRVIADPATTLRLPELAMGLVPGAGGTSSVVRRVGRERAAFLSLTGWPLDAATAANWGLVD
EVRPVHGDARPPV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=169, Percent_Identity=30.1775147928994, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787659, Length=179, Percent_Identity=30.7262569832402, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=127, Percent_Identity=35.4330708661417, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=185, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=208, Percent_Identity=25.4807692307692, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=181, Percent_Identity=27.0718232044199, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=169, Percent_Identity=27.810650887574, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24653477, Length=169, Percent_Identity=27.810650887574, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 42978; Mature: 42847

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVDVRQDQAGARPTGRGSTTIVPGVAAPNVAAANVADVAVLEVDVLEPEEALLLLRAD
CCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHEEEEEEEEEECCCCEEEEEECC
PEMFAAHTGRPLLAVRAHGPLAVEVGELARRLPCVLVGVAPDLADLPSGIESAGYDVLLT
CHHHHHCCCCCEEEEECCCCEEEEHHHHHHHCCEEEEECCCCHHHCCCCHHHCCCEEEEE
GEATPPAPWVGPLRPGDGFTAALGAIAAMTRAVPGACVAFTQLVRYSAGIDVRDAVIAES
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
FTYSTLQGGPEFAAWLAEHRAVRERREAGAATAPVRAEPTGQPTEPAVLVERDGDELWVV
CCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
LNRPRVHNAVSRDLRDGLAAAFALAGADPTVAEVRLRGAGRSFCSGGDLTEFGSLPDPAR
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCHHH
AHAVRTTRSIPLSLLRCARRVTAYMHGTCVGAGVEVPAFARRVIADPATTLRLPELAMGL
HHHHHHHHCCHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHCCCHHHEECHHHHHHC
VPGAGGTSSVVRRVGRERAAFLSLTGWPLDAATAANWGLVDEVRPVHGDARPPV
CCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GAVDVRQDQAGARPTGRGSTTIVPGVAAPNVAAANVADVAVLEVDVLEPEEALLLLRAD
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHEEEEEEEEEECCCCEEEEEECC
PEMFAAHTGRPLLAVRAHGPLAVEVGELARRLPCVLVGVAPDLADLPSGIESAGYDVLLT
CHHHHHCCCCCEEEEECCCCEEEEHHHHHHHCCEEEEECCCCHHHCCCCHHHCCCEEEEE
GEATPPAPWVGPLRPGDGFTAALGAIAAMTRAVPGACVAFTQLVRYSAGIDVRDAVIAES
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
FTYSTLQGGPEFAAWLAEHRAVRERREAGAATAPVRAEPTGQPTEPAVLVERDGDELWVV
CCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEE
LNRPRVHNAVSRDLRDGLAAAFALAGADPTVAEVRLRGAGRSFCSGGDLTEFGSLPDPAR
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCHHH
AHAVRTTRSIPLSLLRCARRVTAYMHGTCVGAGVEVPAFARRVIADPATTLRLPELAMGL
HHHHHHHHCCHHHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHCCCHHHEECHHHHHHC
VPGAGGTSSVVRRVGRERAAFLSLTGWPLDAATAANWGLVDEVRPVHGDARPPV
CCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA