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The map label for this gene is pstI [H]

Identifier: 158314198

GI number: 158314198

Start: 2811528

End: 2812703

Strand: Direct

Name: pstI [H]

Synonym: Franean1_2368

Alternate gene names: 158314198

Gene position: 2811528-2812703 (Clockwise)

Preceding gene: 158314197

Following gene: 158314203

Centisome position: 31.3

GC content: 70.49

Gene sequence:

>1176_bases
GTGAAGAGACATCTACTCCGGGCCGCGGCTACCTGTGGATTCATCGGCGCAGTGGCGATGACCCAGTTAACTGCTCAGGG
TGCCGCGTCCGCGGCGCCTCCCGACCTCACCGGCCGTTACATCGTGGTGCTGAAATCCGCGCCCTCTGCGGCCGCCTCCG
CGGCGGCCGCGACCCGGGCTCGGGATCTCGGCGCCCAGGTGACCCGCGAGTTCCAGCACACGCTGAACGGGTACTCCGCG
CAGCTCGACCCGGCGCAGCTTGCCGCCGTCCGGGCGGATCCCGAGGTCGCCTACGTGGAACCCGACCAGGTGGTGCGGGC
CGATACCGAGCAGCGAACGGCAGACTGGGGCCTGGACCGCATCGACCAGCGCAAGCTCCCACTGAACCGGGCGTACACGT
ACGCCTCGACCGGCGCCAGGGTCACCGCCTACATTGTCGACACCGGTATCCGCACCAGCCACCGGGATTTCGGTGGCCGC
GCCTCCGGCGGTTTCTCCGTCATCGATGACGGTTACGGAACCGAGGACTGCAACGGCCACGGCACGCACGTCGCCGGAAC
GACCGGGGGAACGGCGCACGGCGTCGCCAAGTCGGTGCGGCTCGTCTCGGTGCGTGTTCTGGACTGCGCCGCGTTCGGCA
CCGTCAGCGGCGTCATAGCCGGCGTCGAATGGGTCACCGCCCATCACGGCAGTGGCCCCGCCGTGGTGAACATGAGCCTG
ACCGGCGGCGCGTCGCGGGCGTTCGACCAGGCGGTGCGGCAGTCGATCGCCTCCGGCCTGGTGTACTCGGTGGCCGCGGG
AAACAGCAACGGCGACGCCTGCGCCATCTCGCCCGCGCGGGTGCCCCGGGCGATCACCGTCGGGGCGACGACGACCGCCG
ACAGCCGGGACACCACGTACTCCAACTTCGGCTCCTGCGTGGACGTCTTCGCTCCGGGGACCGGGATCACCTCGGACTGG
AACACCTCCGACACCGCCACCAACACCATCAGCGGGACGTCGATGGCGACCCCGCACGTCACCGGTGTGGCGGCGCTCTA
CCTGCAGCAGCATCCCGGCGCCGGGCCCAACAAGGTGCGCGACGCGATCGTGGACACCGCGACCCGCGGCGCGCTGACCA
ACATCGGCGCCGGCTCGCCGAACCTGCTGCTCTACTCACGCGGGTCGGGCTTCTGA

Upstream 100 bases:

>100_bases
GTCAGCGAAAGCGAACCGAAGTTCGGCTTCCAACCACCAAGCGGAATCACGCGCGACGAGGCCGGATATCCTCCGGCCGG
GTTAGGAGAAGTACGCAGCA

Downstream 100 bases:

>100_bases
CCCATCAAGATAGTCAACAATGTTGACGTTCCGCCTCGAGCAAAAACGATCTTGAAAACCTGAGGCAGCCCAGTACAAGG
CCGGGCTCGGCGGGGGAGGC

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Aqualysin-I [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKRHLLRAAATCGFIGAVAMTQLTAQGAASAAPPDLTGRYIVVLKSAPSAAASAAAATRARDLGAQVTREFQHTLNGYSA
QLDPAQLAAVRADPEVAYVEPDQVVRADTEQRTADWGLDRIDQRKLPLNRAYTYASTGARVTAYIVDTGIRTSHRDFGGR
ASGGFSVIDDGYGTEDCNGHGTHVAGTTGGTAHGVAKSVRLVSVRVLDCAAFGTVSGVIAGVEWVTAHHGSGPAVVNMSL
TGGASRAFDQAVRQSIASGLVYSVAAGNSNGDACAISPARVPRAITVGATTTADSRDTTYSNFGSCVDVFAPGTGITSDW
NTSDTATNTISGTSMATPHVTGVAALYLQQHPGAGPNKVRDAIVDTATRGALTNIGAGSPNLLLYSRGSGF

Sequences:

>Translated_391_residues
MKRHLLRAAATCGFIGAVAMTQLTAQGAASAAPPDLTGRYIVVLKSAPSAAASAAAATRARDLGAQVTREFQHTLNGYSA
QLDPAQLAAVRADPEVAYVEPDQVVRADTEQRTADWGLDRIDQRKLPLNRAYTYASTGARVTAYIVDTGIRTSHRDFGGR
ASGGFSVIDDGYGTEDCNGHGTHVAGTTGGTAHGVAKSVRLVSVRVLDCAAFGTVSGVIAGVEWVTAHHGSGPAVVNMSL
TGGASRAFDQAVRQSIASGLVYSVAAGNSNGDACAISPARVPRAITVGATTTADSRDTTYSNFGSCVDVFAPGTGITSDW
NTSDTATNTISGTSMATPHVTGVAALYLQQHPGAGPNKVRDAIVDTATRGALTNIGAGSPNLLLYSRGSGF
>Mature_391_residues
MKRHLLRAAATCGFIGAVAMTQLTAQGAASAAPPDLTGRYIVVLKSAPSAAASAAAATRARDLGAQVTREFQHTLNGYSA
QLDPAQLAAVRADPEVAYVEPDQVVRADTEQRTADWGLDRIDQRKLPLNRAYTYASTGARVTAYIVDTGIRTSHRDFGGR
ASGGFSVIDDGYGTEDCNGHGTHVAGTTGGTAHGVAKSVRLVSVRVLDCAAFGTVSGVIAGVEWVTAHHGSGPAVVNMSL
TGGASRAFDQAVRQSIASGLVYSVAAGNSNGDACAISPARVPRAITVGATTTADSRDTTYSNFGSCVDVFAPGTGITSDW
NTSDTATNTISGTSMATPHVTGVAALYLQQHPGAGPNKVRDAIVDTATRGALTNIGAGSPNLLLYSRGSGF

Specific function: Aqualysin I is a thermophilic alkaline serine protease [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI31317307, Length=363, Percent_Identity=34.1597796143251, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324576, Length=338, Percent_Identity=38.4615384615385, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320775, Length=354, Percent_Identity=39.5480225988701, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319893, Length=275, Percent_Identity=38.9090909090909, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.111 [H]

Molecular weight: Translated: 40027; Mature: 40027

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00136 SUBTILASE_ASP ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHLLRAAATCGFIGAVAMTQLTAQGAASAAPPDLTGRYIVVLKSAPSAAASAAAATRA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RDLGAQVTREFQHTLNGYSAQLDPAQLAAVRADPEVAYVEPDQVVRADTEQRTADWGLDR
HHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEECCHHHHHCCCHHHHHHCCCCH
IDQRKLPLNRAYTYASTGARVTAYIVDTGIRTSHRDFGGRASGGFSVIDDGYGTEDCNGH
HHHCCCCCHHEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
GTHVAGTTGGTAHGVAKSVRLVSVRVLDCAAFGTVSGVIAGVEWVTAHHGSGPAVVNMSL
CCEEECCCCCCHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEE
TGGASRAFDQAVRQSIASGLVYSVAAGNSNGDACAISPARVPRAITVGATTTADSRDTTY
CCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECHHHCCCEEEECCCCCCCCCCCCH
SNFGSCVDVFAPGTGITSDWNTSDTATNTISGTSMATPHVTGVAALYLQQHPGAGPNKVR
HHCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHEEEEEECCCCCCHHHHH
DAIVDTATRGALTNIGAGSPNLLLYSRGSGF
HHHHHHHHCCCHHCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MKRHLLRAAATCGFIGAVAMTQLTAQGAASAAPPDLTGRYIVVLKSAPSAAASAAAATRA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
RDLGAQVTREFQHTLNGYSAQLDPAQLAAVRADPEVAYVEPDQVVRADTEQRTADWGLDR
HHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEECCHHHHHCCCHHHHHHCCCCH
IDQRKLPLNRAYTYASTGARVTAYIVDTGIRTSHRDFGGRASGGFSVIDDGYGTEDCNGH
HHHCCCCCHHEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
GTHVAGTTGGTAHGVAKSVRLVSVRVLDCAAFGTVSGVIAGVEWVTAHHGSGPAVVNMSL
CCEEECCCCCCHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEE
TGGASRAFDQAVRQSIASGLVYSVAAGNSNGDACAISPARVPRAITVGATTTADSRDTTY
CCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECHHHCCCEEEECCCCCCCCCCCCH
SNFGSCVDVFAPGTGITSDWNTSDTATNTISGTSMATPHVTGVAALYLQQHPGAGPNKVR
HHCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHEEEEEECCCCCCHHHHH
DAIVDTATRGALTNIGAGSPNLLLYSRGSGF
HHHHHHHHCCCHHCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2182621; 3286255; 3162211 [H]