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The map label for this gene is tcmA [H]

Identifier: 158314085

GI number: 158314085

Start: 2691316

End: 2692905

Strand: Direct

Name: tcmA [H]

Synonym: Franean1_2252

Alternate gene names: 158314085

Gene position: 2691316-2692905 (Clockwise)

Preceding gene: 158314084

Following gene: 158314086

Centisome position: 29.96

GC content: 76.86

Gene sequence:

>1590_bases
GTGGCCGTCGAAGCTGGCGAAGTCGGCCGACTGACCCTCACCGGCCGCCAGGGACGCTGGGTCGTCGCGGCGGCGGTGCT
GGGCTCCGCACTGGCGTCGATCGACGCGACCGTCGTCGGCATCGCCCTGCCGGCGCTCGGGCGCGCGTTCGACGCCGACC
TTGCCGCCCTGCAGTGGGTGGTCAACGGCTACGCGCTGGCGCTCGCCGGACTGCTGCTCGTCGGCGGCTCGCTCGGCGAC
CGCTACGGGCGGCGGCGGGTCTTCGTGGTCGGCGTGGTCTGGTTCGCGCTGATGTCCCTGGTGTGTGGGCTGGCACCGAA
CGTGGAGATCCTCGTCGCCGCCCGGGCGCTGCAGGGCGTCGGCGCGGCCCTGCTCACCCCGGCCAGCCTGGCGATCCTGC
AGGCGTCGTTCGACGAGGCCGACCGCGGCCGGGCGATCGGCGCGTGGACGGGGCTCGGCGGGGTGGCCACGGCCGCCGGA
CCCTTCCTCGGCGGCTGGCTCATCGAGGCGTTCTCCTGGCGGCTGATCTTCCTGATCAACCTGCCGCTGGCCGCGGTCGT
GGTCGCGGTCGCGGTGCGCCACGTCCCCGAGAGCCACAACCCTGACGTCCGCGGGCGGCCCGACCTGCTCGGCGGCGCCC
TGGTGACCGCCGGTCTGGTCGGCGTCGTGTTCGCGCTCATCGACGGCCCGGCCAGCGGCTGGACGTCCGCCCCGGTGCTG
GTCGGGCTGGTCGGCGGGGTCGCGCTGCTGGCCGGGTTCGTCGCCCGCGAACACCGGGCGGCCGACCCGGTGGTCCCGCT
GGAGATGTTCGCCTCCCGGCAGTTCACGGCCGCGAACGCCGTCACCTTCCTGCAGTACGCGGCGCTGGGCGGGGCGCTGT
TCCTGCTGCCGATTCAGCTCCAGCAGGTCAGCGGCTTCAGCCCGCTCGCCGCCGGGACGGCGCTGCTGCCTGTCACCGTG
ATGATGCTGCTGTTCTCCGCGCGGGCCGGCGCGCTGGCCACCCGGATCGGGCCGCGGCTGCCGATGACGGTCGGCCCCGT
CGTCGCCGGCGCCGGCCTGCTGCTGGTCACCCGGGCCGGCCCGGACGCCTTCTACCTCACGGACGTCCTGCCCGGTGCCG
TCGTGCTGGGCCTCGGGCTCGCCACCTGCGTGGCCCCGTTGACGTCCACCACGCTCGCCGCGGTGCCGGCGCATCATGCC
GGGGCGGCGTCGGCGGTGAACAATGACGTCGCGCGCACCGGCGGCCTGGTCGCCGTCGCCGTGCTGCCCGCCCTCGCCGG
GCTGGGTGGCACCGCCTACCTCGACCCGGCCCTGTTCGACCGCGGGTTCCGCCGGGCGATGGTCATCTCGGCGCTGGTGT
GCGCGGCCGGCGGTCTGCTCGCCGCGCTCGGTATCCGCAACCCGCGCCGGCCGGCCGCCGAGCTGCCGGCCGCCGAGCGG
GCCTCCGCGCGGATCGGGCGGCAGGCGGGGGAGCGGGTCTGCTGCCCACTGGACGCACCCGGCCTGCGTGCCACACCGGG
CCCGCGGGGCACCCCGGGCCCGGGTGCCACCTCGGGCCCGCCCGCCGTCCCGGGCGGGCCCGCCGGCTGA

Upstream 100 bases:

>100_bases
AAGTGGGATTCTCGGTGGCCTTCGGCGTCCTGCTGGACACGATCGTCGTCCGCTCCATCCTGGTGCCGGCGCTCAGCTAC
GACATCGGGAAGAAGATCTG

Downstream 100 bases:

>100_bases
CCCCAGAGTGGAATGCCGGCCGGCCCCGTGGTTGGCTCAGACCAATGGACGACACGAAGATCTTCGAGCGCATCGACTCC
CTGGTGGCGGAGGAGCACGA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAVEAGEVGRLTLTGRQGRWVVAAAVLGSALASIDATVVGIALPALGRAFDADLAALQWVVNGYALALAGLLLVGGSLGD
RYGRRRVFVVGVVWFALMSLVCGLAPNVEILVAARALQGVGAALLTPASLAILQASFDEADRGRAIGAWTGLGGVATAAG
PFLGGWLIEAFSWRLIFLINLPLAAVVVAVAVRHVPESHNPDVRGRPDLLGGALVTAGLVGVVFALIDGPASGWTSAPVL
VGLVGGVALLAGFVAREHRAADPVVPLEMFASRQFTAANAVTFLQYAALGGALFLLPIQLQQVSGFSPLAAGTALLPVTV
MMLLFSARAGALATRIGPRLPMTVGPVVAGAGLLLVTRAGPDAFYLTDVLPGAVVLGLGLATCVAPLTSTTLAAVPAHHA
GAASAVNNDVARTGGLVAVAVLPALAGLGGTAYLDPALFDRGFRRAMVISALVCAAGGLLAALGIRNPRRPAAELPAAER
ASARIGRQAGERVCCPLDAPGLRATPGPRGTPGPGATSGPPAVPGGPAG

Sequences:

>Translated_529_residues
MAVEAGEVGRLTLTGRQGRWVVAAAVLGSALASIDATVVGIALPALGRAFDADLAALQWVVNGYALALAGLLLVGGSLGD
RYGRRRVFVVGVVWFALMSLVCGLAPNVEILVAARALQGVGAALLTPASLAILQASFDEADRGRAIGAWTGLGGVATAAG
PFLGGWLIEAFSWRLIFLINLPLAAVVVAVAVRHVPESHNPDVRGRPDLLGGALVTAGLVGVVFALIDGPASGWTSAPVL
VGLVGGVALLAGFVAREHRAADPVVPLEMFASRQFTAANAVTFLQYAALGGALFLLPIQLQQVSGFSPLAAGTALLPVTV
MMLLFSARAGALATRIGPRLPMTVGPVVAGAGLLLVTRAGPDAFYLTDVLPGAVVLGLGLATCVAPLTSTTLAAVPAHHA
GAASAVNNDVARTGGLVAVAVLPALAGLGGTAYLDPALFDRGFRRAMVISALVCAAGGLLAALGIRNPRRPAAELPAAER
ASARIGRQAGERVCCPLDAPGLRATPGPRGTPGPGATSGPPAVPGGPAG
>Mature_528_residues
AVEAGEVGRLTLTGRQGRWVVAAAVLGSALASIDATVVGIALPALGRAFDADLAALQWVVNGYALALAGLLLVGGSLGDR
YGRRRVFVVGVVWFALMSLVCGLAPNVEILVAARALQGVGAALLTPASLAILQASFDEADRGRAIGAWTGLGGVATAAGP
FLGGWLIEAFSWRLIFLINLPLAAVVVAVAVRHVPESHNPDVRGRPDLLGGALVTAGLVGVVFALIDGPASGWTSAPVLV
GLVGGVALLAGFVAREHRAADPVVPLEMFASRQFTAANAVTFLQYAALGGALFLLPIQLQQVSGFSPLAAGTALLPVTVM
MLLFSARAGALATRIGPRLPMTVGPVVAGAGLLLVTRAGPDAFYLTDVLPGAVVLGLGLATCVAPLTSTTLAAVPAHHAG
AASAVNNDVARTGGLVAVAVLPALAGLGGTAYLDPALFDRGFRRAMVISALVCAAGGLLAALGIRNPRRPAAELPAAERA
SARIGRQAGERVCCPLDAPGLRATPGPRGTPGPGATSGPPAVPGGPAG

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=410, Percent_Identity=27.3170731707317, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081983, Length=381, Percent_Identity=28.3464566929134, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789042, Length=411, Percent_Identity=26.7639902676399, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=28.4037558685446, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788392, Length=423, Percent_Identity=27.4231678486998, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1790146, Length=146, Percent_Identity=30.1369863013699, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=389, Percent_Identity=25.4498714652956, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323735, Length=304, Percent_Identity=25.6578947368421, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319770, Length=274, Percent_Identity=27.007299270073, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=371, Percent_Identity=23.4501347708895, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=28.1879194630872, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=460, Percent_Identity=21.0869565217391, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323935, Length=438, Percent_Identity=21.4611872146119, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52910; Mature: 52778

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEAGEVGRLTLTGRQGRWVVAAAVLGSALASIDATVVGIALPALGRAFDADLAALQWV
CCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VNGYALALAGLLLVGGSLGDRYGRRRVFVVGVVWFALMSLVCGLAPNVEILVAARALQGV
HHHHHHHHHHHHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHC
GAALLTPASLAILQASFDEADRGRAIGAWTGLGGVATAAGPFLGGWLIEAFSWRLIFLIN
CHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
LPLAAVVVAVAVRHVPESHNPDVRGRPDLLGGALVTAGLVGVVFALIDGPASGWTSAPVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VGLVGGVALLAGFVAREHRAADPVVPLEMFASRQFTAANAVTFLQYAALGGALFLLPIQL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEHHH
QQVSGFSPLAAGTALLPVTVMMLLFSARAGALATRIGPRLPMTVGPVVAGAGLLLVTRAG
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHCCCEEEEEECC
PDAFYLTDVLPGAVVLGLGLATCVAPLTSTTLAAVPAHHAGAASAVNNDVARTGGLVAVA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCHHHHH
VLPALAGLGGTAYLDPALFDRGFRRAMVISALVCAAGGLLAALGIRNPRRPAAELPAAER
HHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHH
ASARIGRQAGERVCCPLDAPGLRATPGPRGTPGPGATSGPPAVPGGPAG
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVEAGEVGRLTLTGRQGRWVVAAAVLGSALASIDATVVGIALPALGRAFDADLAALQWV
CCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VNGYALALAGLLLVGGSLGDRYGRRRVFVVGVVWFALMSLVCGLAPNVEILVAARALQGV
HHHHHHHHHHHHHHCCCHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHC
GAALLTPASLAILQASFDEADRGRAIGAWTGLGGVATAAGPFLGGWLIEAFSWRLIFLIN
CHHHHCHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
LPLAAVVVAVAVRHVPESHNPDVRGRPDLLGGALVTAGLVGVVFALIDGPASGWTSAPVL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VGLVGGVALLAGFVAREHRAADPVVPLEMFASRQFTAANAVTFLQYAALGGALFLLPIQL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEHHH
QQVSGFSPLAAGTALLPVTVMMLLFSARAGALATRIGPRLPMTVGPVVAGAGLLLVTRAG
HHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHCCCEEEEEECC
PDAFYLTDVLPGAVVLGLGLATCVAPLTSTTLAAVPAHHAGAASAVNNDVARTGGLVAVA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCHHHHH
VLPALAGLGGTAYLDPALFDRGFRRAMVISALVCAAGGLLAALGIRNPRRPAAELPAAER
HHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHH
ASARIGRQAGERVCCPLDAPGLRATPGPRGTPGPGATSGPPAVPGGPAG
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]