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The map label for this gene is pyk [H]

Identifier: 158314044

GI number: 158314044

Start: 2644455

End: 2645870

Strand: Direct

Name: pyk [H]

Synonym: Franean1_2211

Alternate gene names: 158314044

Gene position: 2644455-2645870 (Clockwise)

Preceding gene: 158314042

Following gene: 158314046

Centisome position: 29.44

GC content: 71.12

Gene sequence:

>1416_bases
GTGCCACGAAGAGCCAAGATAGTTTGCACCCTCGGCCCGGCCACCAACTCTCCCGAGATGGTCCGGGCCCTCGTCGACGC
CGGAATGGACATTGCCCGGCTCAACTTTTCGCACGGAACGCACGAGCAGCACGCCGCTGCGTACGCCCTGGTGCGCGAGG
CGGCCGCGGCCGCCGGCCGCAGCGTGGGGATCCTGGCCGACCTGCAGGGGCCGAAGATCCGCCTCGGCAGGTTCGCCGAC
GGCGGGGTCACCCTGCTGCCCGGGCAGCATTTCACCGTCACCATCCGTGACGTCCTGGGAGATCAGGAACAGGTCGGCAC
CACGTACCCGGGCCTGACCCGGGACGTCGCCGCCGGGGACACCATCCTCGTCGACGACGGCCGGCTGAACCTGCGGATCG
ACGGGGTCGAGGACACCGACGTCCGCACCACCGTCGTGATCGGCGGCAAGGTCAGCGACAACAAGGGGATGAACATCCCG
AGCGCCGCGCTGACGGTGCCGGCGCTGTCCGAGAAGGACGCCGACGACCTGCGCTTCGCGCTCGAGCTCGGCGTCGACAT
GATCGCGCTGTCGTTCGTCCGGTCCGCCGAGGACTACGCGGCCGTCCGCTCGATCATGGACGAGGTCGGGATCCGGGTTC
CCGTCCTCGCGAAGATCGAGAAGCCGCAGGCCGTCGACGAGCTCGACGGGATCATCGAGGCCTTCGACGGCCTCATGATC
GCCCGCGGTGACCTCGGTGTCGAGCTGCCGCTCGAGGACGTCCCCCTCGTCCAGAAGCGGGCCGTGAGCCAGGCGCGTGA
GCGGGCCAAGCCGGTCATCGTCGCCACCCAGGTGCTCGAGTCGATGGTGAGCGCGCCCCGGCCGACCCGCGCGGAGGCGA
GCGACTGCGCCAACGCCGTGCTGGACGGTGCCGACGCGATCATGCTTTCGGCCGAGACCAGTGTCGGGTCGTATCCGGTC
GAGTCGGCCGCGACCATGGCGCGGATCATCGAGACCGCCGAGGCGGATCTCGGCCGCATCCCCCCGCTGCGGACCCCGCC
GCGCACCCTCGGCGGCGCGATCGCCCAGGCGGCGGTGAGTGTCGGGGAGGCGGTCGGTGCCCGCTACCTGGTGGCCCACA
CCCTCAGCGGGGACACCGCCCGGCGGCTGATCCGATACCGCAGCTCGGTGCCCGTCATCGCCTTCACCCCCATTCCCAGC
GTGCGCAGCCAGATGGCGTTGCTGTGGGGTGTGGAGACGGTGCTGGTGCCGTTCGCGGAGAGCACCGACGAGATGGTCCG
GCAGGTCGACACGGCCCTGCAGCAGGTCGGGCACTGCCGGCCCGGCGAGCTGGTGGTCGTCGTCGCCGGTACTCCGCCGG
GCGTCGCCGGCATGACCAACGCGATGCGGGTGCACCGCATCGGCGAGGCGGTCTGA

Upstream 100 bases:

>100_bases
TCACAGAACCTCTCGGGACTAAGTGGCCCAGAACCGCCGGATCGAGTGTGTCACCAGGGCCCATGTCCTATCCCGGGCGT
TCGTTACCTAGGCTTTGACC

Downstream 100 bases:

>100_bases
CCGGGCCCGCGGGCCCGTGCCCGCCGCGGGAGTGCCGCCGCCGGTCGCTCGCGATCGGTGGCCCGTGGCGGGCTGGAGCG
GCACGATCGTGGTGGGGCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPRRAKIVCTLGPATNSPEMVRALVDAGMDIARLNFSHGTHEQHAAAYALVREAAAAAGRSVGILADLQGPKIRLGRFAD
GGVTLLPGQHFTVTIRDVLGDQEQVGTTYPGLTRDVAAGDTILVDDGRLNLRIDGVEDTDVRTTVVIGGKVSDNKGMNIP
SAALTVPALSEKDADDLRFALELGVDMIALSFVRSAEDYAAVRSIMDEVGIRVPVLAKIEKPQAVDELDGIIEAFDGLMI
ARGDLGVELPLEDVPLVQKRAVSQARERAKPVIVATQVLESMVSAPRPTRAEASDCANAVLDGADAIMLSAETSVGSYPV
ESAATMARIIETAEADLGRIPPLRTPPRTLGGAIAQAAVSVGEAVGARYLVAHTLSGDTARRLIRYRSSVPVIAFTPIPS
VRSQMALLWGVETVLVPFAESTDEMVRQVDTALQQVGHCRPGELVVVVAGTPPGVAGMTNAMRVHRIGEAV

Sequences:

>Translated_471_residues
MPRRAKIVCTLGPATNSPEMVRALVDAGMDIARLNFSHGTHEQHAAAYALVREAAAAAGRSVGILADLQGPKIRLGRFAD
GGVTLLPGQHFTVTIRDVLGDQEQVGTTYPGLTRDVAAGDTILVDDGRLNLRIDGVEDTDVRTTVVIGGKVSDNKGMNIP
SAALTVPALSEKDADDLRFALELGVDMIALSFVRSAEDYAAVRSIMDEVGIRVPVLAKIEKPQAVDELDGIIEAFDGLMI
ARGDLGVELPLEDVPLVQKRAVSQARERAKPVIVATQVLESMVSAPRPTRAEASDCANAVLDGADAIMLSAETSVGSYPV
ESAATMARIIETAEADLGRIPPLRTPPRTLGGAIAQAAVSVGEAVGARYLVAHTLSGDTARRLIRYRSSVPVIAFTPIPS
VRSQMALLWGVETVLVPFAESTDEMVRQVDTALQQVGHCRPGELVVVVAGTPPGVAGMTNAMRVHRIGEAV
>Mature_470_residues
PRRAKIVCTLGPATNSPEMVRALVDAGMDIARLNFSHGTHEQHAAAYALVREAAAAAGRSVGILADLQGPKIRLGRFADG
GVTLLPGQHFTVTIRDVLGDQEQVGTTYPGLTRDVAAGDTILVDDGRLNLRIDGVEDTDVRTTVVIGGKVSDNKGMNIPS
AALTVPALSEKDADDLRFALELGVDMIALSFVRSAEDYAAVRSIMDEVGIRVPVLAKIEKPQAVDELDGIIEAFDGLMIA
RGDLGVELPLEDVPLVQKRAVSQARERAKPVIVATQVLESMVSAPRPTRAEASDCANAVLDGADAIMLSAETSVGSYPVE
SAATMARIIETAEADLGRIPPLRTPPRTLGGAIAQAAVSVGEAVGARYLVAHTLSGDTARRLIRYRSSVPVIAFTPIPSV
RSQMALLWGVETVLVPFAESTDEMVRQVDTALQQVGHCRPGELVVVVAGTPPGVAGMTNAMRVHRIGEAV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=39.2638036809816, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=39.0946502057613, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=39.0946502057613, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=40.8536585365854, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=40.8536585365854, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.0243902439024, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.0243902439024, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=36.5957446808511, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=36.5957446808511, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=35.981308411215, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=39.1489361702128, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1788160, Length=475, Percent_Identity=40.2105263157895, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=39.2197125256673, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=38.9344262295082, Blast_Score=288, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=38.9344262295082, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=38.9344262295082, Blast_Score=287, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=38.9344262295082, Blast_Score=287, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=35.8178053830228, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=35.3909465020576, Blast_Score=266, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=39.6728016359918, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=39.6728016359918, Blast_Score=302, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=37.621359223301, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=33.1262939958592, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24646914, Length=275, Percent_Identity=36, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 49671; Mature: 49539

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRAKIVCTLGPATNSPEMVRALVDAGMDIARLNFSHGTHEQHAAAYALVREAAAAAGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
SVGILADLQGPKIRLGRFADGGVTLLPGQHFTVTIRDVLGDQEQVGTTYPGLTRDVAAGD
CEEEEEECCCCCEEEEEECCCCEEEECCCEEEEEHHHHHCCHHHCCCCCCCCCHHHCCCC
TILVDDGRLNLRIDGVEDTDVRTTVVIGGKVSDNKGMNIPSAALTVPALSEKDADDLRFA
EEEEECCEEEEEECCCCCCCCEEEEEECCEECCCCCCCCCHHHEECCCCCCCCHHHHHHH
LELGVDMIALSFVRSAEDYAAVRSIMDEVGIRVPVLAKIEKPQAVDELDGIIEAFDGLMI
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCEEE
ARGDLGVELPLEDVPLVQKRAVSQARERAKPVIVATQVLESMVSAPRPTRAEASDCANAV
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGADAIMLSAETSVGSYPVESAATMARIIETAEADLGRIPPLRTPPRTLGGAIAQAAVS
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VGEAVGARYLVAHTLSGDTARRLIRYRSSVPVIAFTPIPSVRSQMALLWGVETVLVPFAE
HHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEECCC
STDEMVRQVDTALQQVGHCRPGELVVVVAGTPPGVAGMTNAMRVHRIGEAV
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRRAKIVCTLGPATNSPEMVRALVDAGMDIARLNFSHGTHEQHAAAYALVREAAAAAGR
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
SVGILADLQGPKIRLGRFADGGVTLLPGQHFTVTIRDVLGDQEQVGTTYPGLTRDVAAGD
CEEEEEECCCCCEEEEEECCCCEEEECCCEEEEEHHHHHCCHHHCCCCCCCCCHHHCCCC
TILVDDGRLNLRIDGVEDTDVRTTVVIGGKVSDNKGMNIPSAALTVPALSEKDADDLRFA
EEEEECCEEEEEECCCCCCCCEEEEEECCEECCCCCCCCCHHHEECCCCCCCCHHHHHHH
LELGVDMIALSFVRSAEDYAAVRSIMDEVGIRVPVLAKIEKPQAVDELDGIIEAFDGLMI
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCEEE
ARGDLGVELPLEDVPLVQKRAVSQARERAKPVIVATQVLESMVSAPRPTRAEASDCANAV
EECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
LDGADAIMLSAETSVGSYPVESAATMARIIETAEADLGRIPPLRTPPRTLGGAIAQAAVS
HCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
VGEAVGARYLVAHTLSGDTARRLIRYRSSVPVIAFTPIPSVRSQMALLWGVETVLVPFAE
HHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHEEECCC
STDEMVRQVDTALQQVGHCRPGELVVVVAGTPPGVAGMTNAMRVHRIGEAV
CHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA