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The map label for this gene is pyrB

Identifier: 158313542

GI number: 158313542

Start: 2037609

End: 2038538

Strand: Direct

Name: pyrB

Synonym: Franean1_1706

Alternate gene names: 158313542

Gene position: 2037609-2038538 (Clockwise)

Preceding gene: 158313541

Following gene: 158313543

Centisome position: 22.69

GC content: 70.43

Gene sequence:

>930_bases
ATGAACCATCTGCTTTCCACCGCGGATCTCAAGCTCGACGACGCGCTGCTGGTGCTCGACACGGCGGACCGCATGGCGCG
GGTCGCGGACGCGCCGGTCCGCAAGCTGCCGACGCTGCGCGGCCGGACCGTGGTCAATCTGTTCTTCGAGGACTCCACCC
GTACCCGCACGTCCTTCGAGGTCGCCGCCAAGAGGCTGTCGGCAGATGTGATCAATTTTTCGGCCCGGGGGTCAAGCGTG
TCGAAGGGCGAGAGCCTGAAGGACACAGCGCAGACCCTCGAGGCGATGGGCGCGGACGCCGTCGTCTGCCGTCATCCCGC
GAGCGGCGCCCCGCACCGGCTGGCCAGCTGGGTGCGCGGCAGCGTCGTGAACGCCGGTGACGGTACGCACGAGCACCCCA
CCCAGGCGCTGCTCGACGCCTTCACCATCCGCCGCCGGCTCGGCCGGCTGGACGGGCTGGCCGTGACGATCGTCGGGGAC
GTCCTGCACTCGCGGGTGGCCCGCTCCAATGTCTGGTTGCTGACGACCCTCGGTGCGCGGGTGACCGTGGTCGCCCCGCC
CACGCTGCTGCCGCTCGGTATCTCCGGGTGGCCCGTCGAGGTGTCCTACGACCTCGAGTCGGTCCTGCCCAAGAGCGACG
TGGTCATGATGTTGCGTGTGCAGCGGGAGCGGATGTCCGCGGCGTTCTTCCCGACCGAGCGGGAGTACAGCCGCCGTTAC
GGCCTCGACGCGGACCGGGCCGCGCTCATGCCCGAGCATGCCCTGGTCATGCACCCCGGGCCGATGGTCCGCGGGATGGA
GATCGCCTCCTCGGTGGCCGACTCGCCGCGCTCGACGGTGGTCGAGCAGGTCGCGAACGGCGTGAGCGTCCGCATGGCCG
TCCTCTACCTGCTGCTCGGCGGCAGCGGCGCCGAACGGGAGTCCTCATGA

Upstream 100 bases:

>100_bases
CTCCGGGAGGTCGACGGCGCCGACGCCGTCCTCATCGTCTCGTCGGACGCGCCGTGACCGCGCACGCGACCCCGCGCCGC
CGTCCGAGGTGGTGGGTGTG

Downstream 100 bases:

>100_bases
CATCGGTGATCGTGGTGCGTGGCGTCCGTCCGCTGGGCGGTGACGCGCGGGATCTGGTGATCGCCGGCGGGACGATCGCC
GCTGTCGCGCCGGCCGGGGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNHLLSTADLKLDDALLVLDTADRMARVADAPVRKLPTLRGRTVVNLFFEDSTRTRTSFEVAAKRLSADVINFSARGSSV
SKGESLKDTAQTLEAMGADAVVCRHPASGAPHRLASWVRGSVVNAGDGTHEHPTQALLDAFTIRRRLGRLDGLAVTIVGD
VLHSRVARSNVWLLTTLGARVTVVAPPTLLPLGISGWPVEVSYDLESVLPKSDVVMMLRVQRERMSAAFFPTEREYSRRY
GLDADRAALMPEHALVMHPGPMVRGMEIASSVADSPRSTVVEQVANGVSVRMAVLYLLLGGSGAERESS

Sequences:

>Translated_309_residues
MNHLLSTADLKLDDALLVLDTADRMARVADAPVRKLPTLRGRTVVNLFFEDSTRTRTSFEVAAKRLSADVINFSARGSSV
SKGESLKDTAQTLEAMGADAVVCRHPASGAPHRLASWVRGSVVNAGDGTHEHPTQALLDAFTIRRRLGRLDGLAVTIVGD
VLHSRVARSNVWLLTTLGARVTVVAPPTLLPLGISGWPVEVSYDLESVLPKSDVVMMLRVQRERMSAAFFPTEREYSRRY
GLDADRAALMPEHALVMHPGPMVRGMEIASSVADSPRSTVVEQVANGVSVRMAVLYLLLGGSGAERESS
>Mature_309_residues
MNHLLSTADLKLDDALLVLDTADRMARVADAPVRKLPTLRGRTVVNLFFEDSTRTRTSFEVAAKRLSADVINFSARGSSV
SKGESLKDTAQTLEAMGADAVVCRHPASGAPHRLASWVRGSVVNAGDGTHEHPTQALLDAFTIRRRLGRLDGLAVTIVGD
VLHSRVARSNVWLLTTLGARVTVVAPPTLLPLGISGWPVEVSYDLESVLPKSDVVMMLRVQRERMSAAFFPTEREYSRRY
GLDADRAALMPEHALVMHPGPMVRGMEIASSVADSPRSTVVEQVANGVSVRMAVLYLLLGGSGAERESS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=39.1585760517799, Blast_Score=188, Evalue=5e-48,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=38.3386581469649, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=37.3040752351097, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322331, Length=309, Percent_Identity=36.5695792880259, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24642586, Length=286, Percent_Identity=39.5104895104895, Blast_Score=175, Evalue=4e-44,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_FRASN (A8LE09)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001506050.1
- ProteinModelPortal:   A8LE09
- GeneID:   5670108
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1706
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   FSP1855:FRANEAN1_1706-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33389; Mature: 33389

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLSTADLKLDDALLVLDTADRMARVADAPVRKLPTLRGRTVVNLFFEDSTRTRTSFE
CCCCCCCCCCCCCCEEEEEECHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCCCHHHHH
VAAKRLSADVINFSARGSSVSKGESLKDTAQTLEAMGADAVVCRHPASGAPHRLASWVRG
HHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
SVVNAGDGTHEHPTQALLDAFTIRRRLGRLDGLAVTIVGDVLHSRVARSNVWLLTTLGAR
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCEEEEEECCCE
VTVVAPPTLLPLGISGWPVEVSYDLESVLPKSDVVMMLRVQRERMSAAFFPTEREYSRRY
EEEEECCCEEEECCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
GLDADRAALMPEHALVMHPGPMVRGMEIASSVADSPRSTVVEQVANGVSVRMAVLYLLLG
CCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHC
GSGAERESS
CCCCCCCCC
>Mature Secondary Structure
MNHLLSTADLKLDDALLVLDTADRMARVADAPVRKLPTLRGRTVVNLFFEDSTRTRTSFE
CCCCCCCCCCCCCCEEEEEECHHHHHHHHCCHHHHCCCCCCCEEEEEEEECCCCCHHHHH
VAAKRLSADVINFSARGSSVSKGESLKDTAQTLEAMGADAVVCRHPASGAPHRLASWVRG
HHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHC
SVVNAGDGTHEHPTQALLDAFTIRRRLGRLDGLAVTIVGDVLHSRVARSNVWLLTTLGAR
CEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCEEEEEECCCE
VTVVAPPTLLPLGISGWPVEVSYDLESVLPKSDVVMMLRVQRERMSAAFFPTEREYSRRY
EEEEECCCEEEECCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHC
GLDADRAALMPEHALVMHPGPMVRGMEIASSVADSPRSTVVEQVANGVSVRMAVLYLLLG
CCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHC
GSGAERESS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA