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The map label for this gene is bmr3 [H]

Identifier: 158313345

GI number: 158313345

Start: 1809518

End: 1810891

Strand: Direct

Name: bmr3 [H]

Synonym: Franean1_1507

Alternate gene names: 158313345

Gene position: 1809518-1810891 (Clockwise)

Preceding gene: 158313343

Following gene: 158313346

Centisome position: 20.15

GC content: 72.71

Gene sequence:

>1374_bases
GTGTCCCACCCCCTTGCCGGACCCGCCGATCTTGCCAGTGACGCCGATCCTGCCAGTGACGCCGACGCCGACGCCGACGC
CGACGCCGACGCCGTCACGGCACCGCGGGCGGGTGCCGTCGTGGCGGTTCTGGCGTTCGCCGGGATCGTGGTCGCGTCGA
TGCAGAGCCTGGTGATCCCGCTGCTCCCCGAGCTGCCCGGGCTGGTGCACGCGTCGGCGTCCGGGACGGCTTGGGCGATC
ACCGCGACGCTGCTCGCCTCCGCCATCGCCACTCCGGTGGCGGGCCGGCTCGGGGACATGTACGGCAAGCGGCGCATGCT
GCTGGCCAGCCTCGGCCTGTTGGTGGTCGGCTCGGCCGCCGCCGGCCTGTCCACCACCCTGACCCCCCTGGTCATCGGGC
GGACACTGCAGGGTCTGTCAGCCGGTGTCATCCCGCTGGGGATCAGCATCATGCGCGACGAGCTGCCGCCCGAGCGCCTC
GGTTCGGCGACCGCGACGATGAGCTCCTCGCTCGGTGTTGGCGGCGCCCTGGGCCTGCCCGCGGCGGCCCTGATCGCCGA
CCACACCGACTGGCACCTGCTGTTCTGGATCTCGGCGGGGCTGGGCGTCGTCGCCACGGCACTCGTGCTGCGGCTGGTGC
CGGAATCCCGAGCCCGCACCGGCGGGCGCGTCGACCTGGTCGGTGCGGCAGGGTTGTCGGCCGTGCTCGTGTGCCTGCTG
CTGGCGATCTCCCAGGGGGCCGACTGGGGCTGGGCCAGCGGCCGCACCCTCGGCCTGTTCGTCGCAGGCGTCGCGGTCCT
GCTGGCGTGGGGGCGCTGGGAGCTGCGCGCGCGGCAGCCGCTGGTGGACCTGCGCACCAGCGCGCGACGTCAGGTGCTGG
TCACCAACCTCGCCTCGGTCATGTTCGGTGTCGCCACGATGCCGGTCCGGCTGGTGCAGCCCCAGATACTGCAGCTGCCC
GCCGCCACCGGCTACGGGCTGGGGAAGTCGCTTCTGGTCACCGGCCTGGTCCTGACCCCCACGGGCCTGGTGATGATGGC
CGTCTCACCGCTGTCCGCGCGTATCTCCGCCGCCAGGGGACCGAAGACGACCCTGATGGCCGGAGCCGTCGTGATCGCCG
CCGGCTATGCGCTGGGCATCGGGCTGATGTCCGCCATCTGGCAGCTCATGATGGTCACCAGCGTCATCGGCGCCGGGATC
GGGCTCGCCTACGGCGCCATGCCGGCGCTCATCATGGCGGCGGTTCCGATCTCCGAGACCGGCGCCGCCAACAGCCTCAA
CAGCCTCATGCGGACCATCGGCGCGGCTCTGCTCGCCCTGGCCATCGCCACGCTCGTACCCCGCCGTCGCCCGCTTGTGC
ATGCCGACGCATGA

Upstream 100 bases:

>100_bases
GATTTGTCGCTTCTGTCTGCAAATTTAGTCATCTGATTTAATCCGGTAAATCGCATGAATTAACTGATCTTGCGCTGCTC
TCCCGGTCGGAGGCTCGTCT

Downstream 100 bases:

>100_bases
CGATCCAACATTCCTGATCTTTACCTAAAGAACCCTATAGTGAGGCGTGTTAAGCTGCGTTTGTGGAACGTCTCTACTCG
GTTGATCAAGTTGCCGACCT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSHPLAGPADLASDADPASDADADADADADAVTAPRAGAVVAVLAFAGIVVASMQSLVIPLLPELPGLVHASASGTAWAI
TATLLASAIATPVAGRLGDMYGKRRMLLASLGLLVVGSAAAGLSTTLTPLVIGRTLQGLSAGVIPLGISIMRDELPPERL
GSATATMSSSLGVGGALGLPAAALIADHTDWHLLFWISAGLGVVATALVLRLVPESRARTGGRVDLVGAAGLSAVLVCLL
LAISQGADWGWASGRTLGLFVAGVAVLLAWGRWELRARQPLVDLRTSARRQVLVTNLASVMFGVATMPVRLVQPQILQLP
AATGYGLGKSLLVTGLVLTPTGLVMMAVSPLSARISAARGPKTTLMAGAVVIAAGYALGIGLMSAIWQLMMVTSVIGAGI
GLAYGAMPALIMAAVPISETGAANSLNSLMRTIGAALLALAIATLVPRRRPLVHADA

Sequences:

>Translated_457_residues
MSHPLAGPADLASDADPASDADADADADADAVTAPRAGAVVAVLAFAGIVVASMQSLVIPLLPELPGLVHASASGTAWAI
TATLLASAIATPVAGRLGDMYGKRRMLLASLGLLVVGSAAAGLSTTLTPLVIGRTLQGLSAGVIPLGISIMRDELPPERL
GSATATMSSSLGVGGALGLPAAALIADHTDWHLLFWISAGLGVVATALVLRLVPESRARTGGRVDLVGAAGLSAVLVCLL
LAISQGADWGWASGRTLGLFVAGVAVLLAWGRWELRARQPLVDLRTSARRQVLVTNLASVMFGVATMPVRLVQPQILQLP
AATGYGLGKSLLVTGLVLTPTGLVMMAVSPLSARISAARGPKTTLMAGAVVIAAGYALGIGLMSAIWQLMMVTSVIGAGI
GLAYGAMPALIMAAVPISETGAANSLNSLMRTIGAALLALAIATLVPRRRPLVHADA
>Mature_456_residues
SHPLAGPADLASDADPASDADADADADADAVTAPRAGAVVAVLAFAGIVVASMQSLVIPLLPELPGLVHASASGTAWAIT
ATLLASAIATPVAGRLGDMYGKRRMLLASLGLLVVGSAAAGLSTTLTPLVIGRTLQGLSAGVIPLGISIMRDELPPERLG
SATATMSSSLGVGGALGLPAAALIADHTDWHLLFWISAGLGVVATALVLRLVPESRARTGGRVDLVGAAGLSAVLVCLLL
AISQGADWGWASGRTLGLFVAGVAVLLAWGRWELRARQPLVDLRTSARRQVLVTNLASVMFGVATMPVRLVQPQILQLPA
ATGYGLGKSLLVTGLVLTPTGLVMMAVSPLSARISAARGPKTTLMAGAVVIAAGYALGIGLMSAIWQLMMVTSVIGAGIG
LAYGAMPALIMAAVPISETGAANSLNSLMRTIGAALLALAIATLVPRRRPLVHADA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=404, Percent_Identity=25.4950495049505, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789042, Length=408, Percent_Identity=24.2647058823529, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081983, Length=437, Percent_Identity=27.2311212814645, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790195, Length=373, Percent_Identity=24.9329758713137, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46012; Mature: 45881

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPLAGPADLASDADPASDADADADADADAVTAPRAGAVVAVLAFAGIVVASMQSLVIP
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLPELPGLVHASASGTAWAITATLLASAIATPVAGRLGDMYGKRRMLLASLGLLVVGSAA
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AGLSTTLTPLVIGRTLQGLSAGVIPLGISIMRDELPPERLGSATATMSSSLGVGGALGLP
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCCHHHCC
AAALIADHTDWHLLFWISAGLGVVATALVLRLVPESRARTGGRVDLVGAAGLSAVLVCLL
HHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCHHHHHHHHH
LAISQGADWGWASGRTLGLFVAGVAVLLAWGRWELRARQPLVDLRTSARRQVLVTNLASV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MFGVATMPVRLVQPQILQLPAATGYGLGKSLLVTGLVLTPTGLVMMAVSPLSARISAARG
HHHHHHCCHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCHHHHHHHHCCC
PKTTLMAGAVVIAAGYALGIGLMSAIWQLMMVTSVIGAGIGLAYGAMPALIMAAVPISET
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAANSLNSLMRTIGAALLALAIATLVPRRRPLVHADA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SHPLAGPADLASDADPASDADADADADADAVTAPRAGAVVAVLAFAGIVVASMQSLVIP
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLPELPGLVHASASGTAWAITATLLASAIATPVAGRLGDMYGKRRMLLASLGLLVVGSAA
HHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AGLSTTLTPLVIGRTLQGLSAGVIPLGISIMRDELPPERLGSATATMSSSLGVGGALGLP
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCCHHHCC
AAALIADHTDWHLLFWISAGLGVVATALVLRLVPESRARTGGRVDLVGAAGLSAVLVCLL
HHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHCCHHHCCCCCCEEEEECCCHHHHHHHHH
LAISQGADWGWASGRTLGLFVAGVAVLLAWGRWELRARQPLVDLRTSARRQVLVTNLASV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MFGVATMPVRLVQPQILQLPAATGYGLGKSLLVTGLVLTPTGLVMMAVSPLSARISAARG
HHHHHHCCHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCHHHHHHHHCCC
PKTTLMAGAVVIAAGYALGIGLMSAIWQLMMVTSVIGAGIGLAYGAMPALIMAAVPISET
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAANSLNSLMRTIGAALLALAIATLVPRRRPLVHADA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]