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The map label for this gene is hrdB [H]

Identifier: 158313089

GI number: 158313089

Start: 1484052

End: 1485323

Strand: Direct

Name: hrdB [H]

Synonym: Franean1_1237

Alternate gene names: 158313089

Gene position: 1484052-1485323 (Clockwise)

Preceding gene: 158313087

Following gene: 158313091

Centisome position: 16.52

GC content: 69.89

Gene sequence:

>1272_bases
ATGACGCTGCCTGCCCTGGAACTAGCCGAGCGCACCGACGAGTCACGCCCGCGCCCGCGGCGTACCCGTCGCTCCGCTTC
TCCTGTCCGTAACACTCCAAGCCGCACGCTGGCCGCGGTCCCCGACGAGCTCGACGAGCTCGACGTCTCCGCCATCGCGG
AACTCATCGCGCGTGGCCGCGAGACCGGCGAGCTCAGCCGTTCCGAGCTCCGTGAGGCTCTCGAGGCCGCCGACATCGGC
GTCGAGCTGCTGCCCGCGCTGATCTCCCGCCTGGGTGCCGCCGGGATCGACCTCGTCGAAGAAGAAGAGGAGCGCGTCAC
CCCCGGCGCTCCGGCCGCCGGCCGCACGGTCGCCGACCACGCCGGCACCGCCGACCTCGTCCGCATGTACTTGCGGGAGA
TCGGCAAGGTCCCGCTGCTCAACGCCGCTCAGGAGGTCGAGCTCTCCAAGCGCGTCGAGGCGGGCCTGTTCGCCGAGCAC
AAGCTCGACACCGACCAGGACCTGGCCGACGACCTGCGCCGGGATCTCGGCGTGCTGGTCACCGACGGCCAGGCTGCCAA
GCAGCAGCTGGTCTCGGCCAACCTGCGCCTGGTGGTGTCGGTCGCCAAGAAGTACAGCGGCCGGGGTATGACGCTGCTGG
ACCTCGTCCAGGAGGGAAACCTGGGCCTGATCCGCGCGGTCGAGAAGTTCGACTACGCGAAGGGCTACAAGTTCTCCACC
TACGCCACCTGGTGGATCCGTCAGGCGATCGGCCGCGCGCTGGCCGACCAGGCCCGCACGATCCGTATCCCGGTGCACGT
GGTCGAGCAGATCAACAAGATCACCCGGCTGCAGCGCCAGCTTGTCTCGACCCTCGGCCGCGAGCCGACGGACGAGGAGC
TCGCGCTCGAGCTGGACATGCCGATCGAGCAGGTCGTCGAACTGCGCCGCTACGCGCAGGACACGGTCAGCCTGGAGACG
TCCGTCGGTGACGACGGCGACTCCGTGCTCGGCGACTTCATCGAGGACTCGGACGCGACGTCGCCCGCCGACGCCGCCTC
CTACGGCGCCATGCAGGACGAGATCGACAACGTCCTCGGTGCGCTGAACCCTCGTGAGCGCGAGGTCATGCGGCTGCGTT
TCGGGCTCGCCGACGGGAAGCAGCACACCCTCGCCGAGGTCGGCAACCGGCTCGGGCTCACCCGTGAGCGCATCCGCCAG
ATCGAGCGGGACACGCTGCGGGAGCTGCGCAAGCCGGCCGTGGCCGGAAGGCTGCGCGAGTTCCTCGACTGA

Upstream 100 bases:

>100_bases
CGGCTGTGCCTTCACGGGGAATGGTTGCGTCTGTTCAGTCGTTACCTCTCGTGCAAGCAACTAGACGACCCACCCGCACC
GACCATCGGATCTGCCACTG

Downstream 100 bases:

>100_bases
GGAGCCGGCGTCTGGGACGAGGTCGTCGTGTGGGCGCCGGCATCGCGGGCCGGCACCCATGACCGCCCGGGGTGTGGGCC
ATACGGTCCTGCTCCGGCAG

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTLPALELAERTDESRPRPRRTRRSASPVRNTPSRTLAAVPDELDELDVSAIAELIARGRETGELSRSELREALEAADIG
VELLPALISRLGAAGIDLVEEEEERVTPGAPAAGRTVADHAGTADLVRMYLREIGKVPLLNAAQEVELSKRVEAGLFAEH
KLDTDQDLADDLRRDLGVLVTDGQAAKQQLVSANLRLVVSVAKKYSGRGMTLLDLVQEGNLGLIRAVEKFDYAKGYKFST
YATWWIRQAIGRALADQARTIRIPVHVVEQINKITRLQRQLVSTLGREPTDEELALELDMPIEQVVELRRYAQDTVSLET
SVGDDGDSVLGDFIEDSDATSPADAASYGAMQDEIDNVLGALNPREREVMRLRFGLADGKQHTLAEVGNRLGLTRERIRQ
IERDTLRELRKPAVAGRLREFLD

Sequences:

>Translated_423_residues
MTLPALELAERTDESRPRPRRTRRSASPVRNTPSRTLAAVPDELDELDVSAIAELIARGRETGELSRSELREALEAADIG
VELLPALISRLGAAGIDLVEEEEERVTPGAPAAGRTVADHAGTADLVRMYLREIGKVPLLNAAQEVELSKRVEAGLFAEH
KLDTDQDLADDLRRDLGVLVTDGQAAKQQLVSANLRLVVSVAKKYSGRGMTLLDLVQEGNLGLIRAVEKFDYAKGYKFST
YATWWIRQAIGRALADQARTIRIPVHVVEQINKITRLQRQLVSTLGREPTDEELALELDMPIEQVVELRRYAQDTVSLET
SVGDDGDSVLGDFIEDSDATSPADAASYGAMQDEIDNVLGALNPREREVMRLRFGLADGKQHTLAEVGNRLGLTRERIRQ
IERDTLRELRKPAVAGRLREFLD
>Mature_422_residues
TLPALELAERTDESRPRPRRTRRSASPVRNTPSRTLAAVPDELDELDVSAIAELIARGRETGELSRSELREALEAADIGV
ELLPALISRLGAAGIDLVEEEEERVTPGAPAAGRTVADHAGTADLVRMYLREIGKVPLLNAAQEVELSKRVEAGLFAEHK
LDTDQDLADDLRRDLGVLVTDGQAAKQQLVSANLRLVVSVAKKYSGRGMTLLDLVQEGNLGLIRAVEKFDYAKGYKFSTY
ATWWIRQAIGRALADQARTIRIPVHVVEQINKITRLQRQLVSTLGREPTDEELALELDMPIEQVVELRRYAQDTVSLETS
VGDDGDSVLGDFIEDSDATSPADAASYGAMQDEIDNVLGALNPREREVMRLRFGLADGKQHTLAEVGNRLGLTRERIRQI
ERDTLRELRKPAVAGRLREFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=52.1739130434783, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=42.1602787456446, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789871, Length=253, Percent_Identity=30.0395256916996, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 46751; Mature: 46619

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPALELAERTDESRPRPRRTRRSASPVRNTPSRTLAAVPDELDELDVSAIAELIARGR
CCCCHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCHHHHHCCHHHHHHHHHCCC
ETGELSRSELREALEAADIGVELLPALISRLGAAGIDLVEEEEERVTPGAPAAGRTVADH
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHC
AGTADLVRMYLREIGKVPLLNAAQEVELSKRVEAGLFAEHKLDTDQDLADDLRRDLGVLV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEE
TDGQAAKQQLVSANLRLVVSVAKKYSGRGMTLLDLVQEGNLGLIRAVEKFDYAKGYKFST
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
YATWWIRQAIGRALADQARTIRIPVHVVEQINKITRLQRQLVSTLGREPTDEELALELDM
HHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
PIEQVVELRRYAQDTVSLETSVGDDGDSVLGDFIEDSDATSPADAASYGAMQDEIDNVLG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALNPREREVMRLRFGLADGKQHTLAEVGNRLGLTRERIRQIERDTLRELRKPAVAGRLRE
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLD
HCC
>Mature Secondary Structure 
TLPALELAERTDESRPRPRRTRRSASPVRNTPSRTLAAVPDELDELDVSAIAELIARGR
CCCHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCHHHHHCCHHHHHHHHHCCC
ETGELSRSELREALEAADIGVELLPALISRLGAAGIDLVEEEEERVTPGAPAAGRTVADH
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHC
AGTADLVRMYLREIGKVPLLNAAQEVELSKRVEAGLFAEHKLDTDQDLADDLRRDLGVLV
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEE
TDGQAAKQQLVSANLRLVVSVAKKYSGRGMTLLDLVQEGNLGLIRAVEKFDYAKGYKFST
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHH
YATWWIRQAIGRALADQARTIRIPVHVVEQINKITRLQRQLVSTLGREPTDEELALELDM
HHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
PIEQVVELRRYAQDTVSLETSVGDDGDSVLGDFIEDSDATSPADAASYGAMQDEIDNVLG
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
ALNPREREVMRLRFGLADGKQHTLAEVGNRLGLTRERIRQIERDTLRELRKPAVAGRLRE
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FLD
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8690707 [H]