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The map label for this gene is miaB

Identifier: 158313078

GI number: 158313078

Start: 1467206

End: 1468690

Strand: Direct

Name: miaB

Synonym: Franean1_1226

Alternate gene names: 158313078

Gene position: 1467206-1468690 (Clockwise)

Preceding gene: 158313073

Following gene: 158313079

Centisome position: 16.33

GC content: 72.19

Gene sequence:

>1485_bases
GTGAGCGGCCGTAGCTATCAGGTCCGGACGTTCGGCTGTCAGATGAACGTGCACGACTCCGAACGGCTCGCCGGCCTTCT
CGAGTCCGCCGGCTACGTTCCGGCCCCGGAGGGGTCCGACGCCGACGTCGTCGTGTTCAACACCTGCGCTGTCCGTGAGA
ACGCCGACAACCGGTTGTACGGAAACCTCGGTCATCTGTACCCGGCGAAGAAGAGCAACCCGGCGATGCAGATCGCGGTG
GGTGGCTGCCTCGCGCAGAAGGACCGGTCGGTGATCCTGGATCGCGCGCCCTGGGTCGACGTCGTGTTCGGCACCCACAA
CATCCATCGGCTGCCGGTGCTGCTCGAGCGGGCCCGGCACAACTCCGCCGCCCAGGTGGAGATCGCCGAGGCGCTCGAGG
TCTTCCCGAGCAGCCTGCCGGCCCGCCGGGCCAGCCATCACTCGGCGTGGGTCAGCATCAGTGTGGGCTGCGACAACACC
TGCACCTTCTGCATCGTGCCGAGCCTGCGCGGCGGTGAGCGCGACCGGCGTCCCGGCGACGTGCTCGCCGAGGTGGAGGC
GCTGGTCGCCGAGGGCGCGCTGGAGATAACGCTGCTCGGCCAGAACGTGAACTCCTACGGGCGCTCACTGGGTGACCCGG
GCGCGTTCGCGAAGCTGCTGCGCGCGTGCGGGCGGGTCGAGGGGCTGGAGCGGGTCCGTTTCACCTCACCGCATCCGCGT
GATTTCACCGACGACGTCATCGAGGCGATGGCGCAGACGCCGAACGTGTGCCCACAGCTGCACATGCCGCTGCAGTCGGG
GTCGGACGTGGTGCTGCGCCGGATGCGGCGCAGCTACCGCCGGGAGCGTTTCCTCGGCATCGTCGAGCGGGTCCGCGCGG
CCATGCCGGACGCCGCGATCACCACCGACATCATCGTCGGATTCCCGGGTGAGACCGAGGCCGACTTCGCCGACACCCTG
GACGTCGTCCGTACCGCGCGTTTCGCCGGGGCGTTCACCTTCAAGTACTCCCCGCGTCCCGGCACGCCCGCGGCCGAGAT
GGACGGCGCGGTCGATCCCGCGGTGGTCTCCGAGCGCTACGGGCGGCTCGCGAGCCTGCAGGACGAGATGTCGTGGGCGG
AGAACCGGGCGCAGGTCGGGCGCCGGGTCGAGATCGTCGTGGCAGAGGGGGAGGGGCGCAAGGACTCCGCCACCGGCCGC
CTCTCCGGACGCGCCCGCGACGGCCGCCTCGTCCACCTGCTGGTGACCGGGCCGCATGCGCAGGAGGGGCTCGTCCGGCC
GGGTGACGTCGTGGAGACCGTGGTGACCAGGGGCGCGCCGCACCACCTGACGGCGGACGGCCCGCTGCTCACCCACCGCC
GGACCCGCGCCGGTGATGCCTGGGCCGCCGGCCGCGCCATCGCCGGCGACACCCCGCGCCCGGACCGCCCCGCGGTCTCT
CTGGGGATGCCGCAGCTGCGCCCGAGCGCCCCCGCCGCGCGGTGA

Upstream 100 bases:

>100_bases
GTGCCGCGCCGGTCCCTGCGCTTAACGTGAAGGTCAAGGTTCGAAGGAGTCGATCCCGGCGTGTCGAACGGTGTCCGGGT
GCGGGCCGTAGGCTGGGTGG

Downstream 100 bases:

>100_bases
CCCTGGTCGCCGCGGCTGTCTGCCCGCATCCGCCGCTGCTGGTCCCGCAGGTCGCCGGGGTGGACCCGGTCGAGGTCCGC
GACCATGCCGGCGCGGCCGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLYGNLGHLYPAKKSNPAMQIAV
GGCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARHNSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNT
CTFCIVPSLRGGERDRRPGDVLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPR
DFTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAITTDIIVGFPGETEADFADTL
DVVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERYGRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGR
LSGRARDGRLVHLLVTGPHAQEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVS
LGMPQLRPSAPAAR

Sequences:

>Translated_494_residues
MSGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLYGNLGHLYPAKKSNPAMQIAV
GGCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARHNSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNT
CTFCIVPSLRGGERDRRPGDVLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPR
DFTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAITTDIIVGFPGETEADFADTL
DVVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERYGRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGR
LSGRARDGRLVHLLVTGPHAQEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVS
LGMPQLRPSAPAAR
>Mature_493_residues
SGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLYGNLGHLYPAKKSNPAMQIAVG
GCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARHNSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNTC
TFCIVPSLRGGERDRRPGDVLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPRD
FTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAITTDIIVGFPGETEADFADTLD
VVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERYGRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGRL
SGRARDGRLVHLLVTGPHAQEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVSL
GMPQLRPSAPAAR

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=497, Percent_Identity=34.20523138833, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI28872782, Length=497, Percent_Identity=34.20523138833, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI93277076, Length=403, Percent_Identity=30.5210918114144, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI1786882, Length=419, Percent_Identity=40.3341288782816, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=30.7174887892377, Blast_Score=185, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=32.2510822510823, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=215, Percent_Identity=34.4186046511628, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356207, Length=426, Percent_Identity=36.6197183098592, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19922432, Length=397, Percent_Identity=31.2342569269521, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_FRASN (A8L6J8)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505586.1
- ProteinModelPortal:   A8L6J8
- SMR:   A8L6J8
- GeneID:   5669639
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1226
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14326
- BioCyc:   FSP1855:FRANEAN1_1226-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53439; Mature: 53308

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLY
CCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCEE
GNLGHLYPAKKSNPAMQIAVGGCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARH
CCCCCCCCCCCCCCCEEEEECCHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCC
NSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNTCTFCIVPSLRGGERDRRPGD
CCCCEEHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCHH
VLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPR
HHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAI
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TTDIIVGFPGETEADFADTLDVVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERY
EEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHCCCCCCHHHHHHHH
GRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGRLSGRARDGRLVHLLVTGPHA
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEEEECCCC
QEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVS
CCCCCCCHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEE
LGMPQLRPSAPAAR
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SGRSYQVRTFGCQMNVHDSERLAGLLESAGYVPAPEGSDADVVVFNTCAVRENADNRLY
CCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCEE
GNLGHLYPAKKSNPAMQIAVGGCLAQKDRSVILDRAPWVDVVFGTHNIHRLPVLLERARH
CCCCCCCCCCCCCCCEEEEECCHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCC
NSAAQVEIAEALEVFPSSLPARRASHHSAWVSISVGCDNTCTFCIVPSLRGGERDRRPGD
CCCCEEHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCHH
VLAEVEALVAEGALEITLLGQNVNSYGRSLGDPGAFAKLLRACGRVEGLERVRFTSPHPR
HHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFTDDVIEAMAQTPNVCPQLHMPLQSGSDVVLRRMRRSYRRERFLGIVERVRAAMPDAAI
CCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TTDIIVGFPGETEADFADTLDVVRTARFAGAFTFKYSPRPGTPAAEMDGAVDPAVVSERY
EEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHCCCCCCHHHHHHHH
GRLASLQDEMSWAENRAQVGRRVEIVVAEGEGRKDSATGRLSGRARDGRLVHLLVTGPHA
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEEEECCCC
QEGLVRPGDVVETVVTRGAPHHLTADGPLLTHRRTRAGDAWAAGRAIAGDTPRPDRPAVS
CCCCCCCHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEE
LGMPQLRPSAPAAR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA