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The map label for this gene is ispG [H]

Identifier: 158313022

GI number: 158313022

Start: 1394101

End: 1395255

Strand: Direct

Name: ispG [H]

Synonym: Franean1_1170

Alternate gene names: 158313022

Gene position: 1394101-1395255 (Clockwise)

Preceding gene: 158313021

Following gene: 158313023

Centisome position: 15.52

GC content: 69.52

Gene sequence:

>1155_bases
GTGACCGTAACTCTGGGCATGCCCGCCGCTCCGCCTCGTCCGGTCGGAACGCGGCGGCTCAGCCGGCAGATCCACGTGGG
CAACGTCCCGGTCGGAGGTGACGCCCCGGTCTCCATCCAGTCGATGTGCACCACGCTGACCTCGGACGTCAACGCGACCC
TTCAGCAGATCGCCCAGCTCACCGCCGCCGGATGTCAGATCGTGCGGGTGGCGGTGCCCAGCCAGGACGACGCCGACGCG
CTGCCGACGATCGCCCGCAAGTCGCCGATCCCGATCATCGCCGACATCCACTTCCAGCCGAAGTACGTCTTCGCCGCGAT
CGACGCCGGGTGCGCCGCCGTCCGGGTGAACCCGGGCAACATCAAGCAGTTCGACGACAAGGTCGGCGAGATCGCCCGCG
CCGCCAAGGCGGCCGGCACCCCGATTCGGATCGGGGTCAACGCCGGGTCACTCGACAAGCGCCTGCTGGAGAAGTACGGG
AAGGCCACTCCGGAGGCGCTGGTCGAGTCGGCGCTGTGGGAGGCCTCGCTGTTCGAGGAGCACGACTTCCGGGACATCAA
GATCTCGGTGAAGCACAACGACCCCGTGATCATGATTCAGGCCTACCGGCTGCTGGCCCAGGCCTGTGACTACCCGCTGC
ACCTCGGCGTCACCGAGGCGGGCCCGGCGTTCCAGGGCACGGTGAAGTCCGCGGTGGCCTTCGGCGCGCTGCTGTCCGAG
GGCATCGGCGACACGATCCGGGTCTCGCTGTCGGCGCCGCCCATCGAGGAGATCAAGGTCGCCTCGGCGATCCTGGAGTC
GCTCGGGCTGCGCGAGCGCAAGCTGGAGATCGTCTCCTGCCCGTCCTGCGGCCGCGCCCAGGTGGACGTCTACACCCTGG
CCAACGAGGTCAGCGCCGGCCTGGAGGGCATGGAGGTGCCGCTGCGGGTGGCCGTCATGGGCTGCGTCGTCAACGGCCCC
GGTGAGGCCCGCGAAGCCGACCTCGGCGTTGCCTCCGGCAACGGCAAGGGTCAGATCTTCGTGCGCGGAAAGGTCATCAA
GACGGTGCCCGAGGCGCAGATCGTGGAGACTCTCATCGAGGAGGCCATGCGGCTGGCCGAGCAGATGGAGGCCGACGGCG
CGGCCTCCGGCTCGCCGTCGGTGTCCGTGGCCTAG

Upstream 100 bases:

>100_bases
GCCCCGCACGACGCCGACTGACCCGGACGATCCCGTCCGTGACGGGCCTGGCCGGGTCGCCATCTGACGATCAAGCATCC
GACACGCCAGTGAGGACATC

Downstream 100 bases:

>100_bases
TACCTGGAAGGGCTGGATGGGTCTGCCGCTTCGCTCCGCTCCGACCCGGCTGCTCGACGATCGGGATCTTCCCGCGGTCA
CCGAACTGCTGGGCCGCAAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTVTLGMPAAPPRPVGTRRLSRQIHVGNVPVGGDAPVSIQSMCTTLTSDVNATLQQIAQLTAAGCQIVRVAVPSQDDADA
LPTIARKSPIPIIADIHFQPKYVFAAIDAGCAAVRVNPGNIKQFDDKVGEIARAAKAAGTPIRIGVNAGSLDKRLLEKYG
KATPEALVESALWEASLFEEHDFRDIKISVKHNDPVIMIQAYRLLAQACDYPLHLGVTEAGPAFQGTVKSAVAFGALLSE
GIGDTIRVSLSAPPIEEIKVASAILESLGLRERKLEIVSCPSCGRAQVDVYTLANEVSAGLEGMEVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGKVIKTVPEAQIVETLIEEAMRLAEQMEADGAASGSPSVSVA

Sequences:

>Translated_384_residues
MTVTLGMPAAPPRPVGTRRLSRQIHVGNVPVGGDAPVSIQSMCTTLTSDVNATLQQIAQLTAAGCQIVRVAVPSQDDADA
LPTIARKSPIPIIADIHFQPKYVFAAIDAGCAAVRVNPGNIKQFDDKVGEIARAAKAAGTPIRIGVNAGSLDKRLLEKYG
KATPEALVESALWEASLFEEHDFRDIKISVKHNDPVIMIQAYRLLAQACDYPLHLGVTEAGPAFQGTVKSAVAFGALLSE
GIGDTIRVSLSAPPIEEIKVASAILESLGLRERKLEIVSCPSCGRAQVDVYTLANEVSAGLEGMEVPLRVAVMGCVVNGP
GEAREADLGVASGNGKGQIFVRGKVIKTVPEAQIVETLIEEAMRLAEQMEADGAASGSPSVSVA
>Mature_383_residues
TVTLGMPAAPPRPVGTRRLSRQIHVGNVPVGGDAPVSIQSMCTTLTSDVNATLQQIAQLTAAGCQIVRVAVPSQDDADAL
PTIARKSPIPIIADIHFQPKYVFAAIDAGCAAVRVNPGNIKQFDDKVGEIARAAKAAGTPIRIGVNAGSLDKRLLEKYGK
ATPEALVESALWEASLFEEHDFRDIKISVKHNDPVIMIQAYRLLAQACDYPLHLGVTEAGPAFQGTVKSAVAFGALLSEG
IGDTIRVSLSAPPIEEIKVASAILESLGLRERKLEIVSCPSCGRAQVDVYTLANEVSAGLEGMEVPLRVAVMGCVVNGPG
EAREADLGVASGNGKGQIFVRGKVIKTVPEAQIVETLIEEAMRLAEQMEADGAASGSPSVSVA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=326, Percent_Identity=47.5460122699387, Blast_Score=311, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40241; Mature: 40110

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTLGMPAAPPRPVGTRRLSRQIHVGNVPVGGDAPVSIQSMCTTLTSDVNATLQQIAQL
CEEEECCCCCCCCCCCHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAAGCQIVRVAVPSQDDADALPTIARKSPIPIIADIHFQPKYVFAAIDAGCAAVRVNPGN
HHCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCEEEEECCCC
IKQFDDKVGEIARAAKAAGTPIRIGVNAGSLDKRLLEKYGKATPEALVESALWEASLFEE
CHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
HDFRDIKISVKHNDPVIMIQAYRLLAQACDYPLHLGVTEAGPAFQGTVKSAVAFGALLSE
CCCEEEEEEEECCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPIEEIKVASAILESLGLRERKLEIVSCPSCGRAQVDVYTLANEVSAG
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEHEEHHHHHHCC
LEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGKVIKTVPEAQIVETLIE
CCCCCCHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCHHHHHHHHHH
EAMRLAEQMEADGAASGSPSVSVA
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTLGMPAAPPRPVGTRRLSRQIHVGNVPVGGDAPVSIQSMCTTLTSDVNATLQQIAQL
EEEECCCCCCCCCCCHHHHHCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAAGCQIVRVAVPSQDDADALPTIARKSPIPIIADIHFQPKYVFAAIDAGCAAVRVNPGN
HHCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCEEEEECCCC
IKQFDDKVGEIARAAKAAGTPIRIGVNAGSLDKRLLEKYGKATPEALVESALWEASLFEE
CHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
HDFRDIKISVKHNDPVIMIQAYRLLAQACDYPLHLGVTEAGPAFQGTVKSAVAFGALLSE
CCCEEEEEEEECCCCEEEHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHC
GIGDTIRVSLSAPPIEEIKVASAILESLGLRERKLEIVSCPSCGRAQVDVYTLANEVSAG
CCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEHEEHHHHHHCC
LEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGKVIKTVPEAQIVETLIE
CCCCCCHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEEECEEEECCCHHHHHHHHHH
EAMRLAEQMEADGAASGSPSVSVA
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA