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The map label for this gene is rnhB

Identifier: 158313006

GI number: 158313006

Start: 1376391

End: 1377113

Strand: Direct

Name: rnhB

Synonym: Franean1_1154

Alternate gene names: 158313006

Gene position: 1376391-1377113 (Clockwise)

Preceding gene: 158313005

Following gene: 158313007

Centisome position: 15.32

GC content: 70.12

Gene sequence:

>723_bases
ATGGACGTGCGTGGCACGGCGATCCGCTCCGATGCCGGCCTCTTCGGCTACGAACGGGCGCTCGTCCGGCGCGGGCTCGG
GCCGGTCGCGGGTGTGGACGAGGCCGGCCGGGGAGCCTGCGCCGGGCCGATGGTGGTGGCCGCCGTCGTCCTGGACCAGC
GTCGTCTGGGGCTGCTCAGGCGGCTGGCCGACTCGAAGCTCCTCACCGAGCGGGTCCGCGAGGAGGTCCACGCGGACGTC
CTGGCAGCGGCGGCGGCGGTGTCGACCATCGTCATCCCCGCCGCCGAGATCGATCGGAACGGCGTGCACGTCGCCAACAT
CATGGGGATGCGGCGGGCGGTGGCGGTGCTCGACGTCCGGCCGAGGTATGTGCTCACCGACGGGTTCGCGGTCGCGGGTC
TTGGGGCCGAGTCGCTGGCGGTGATCAAAGGCGACCTGATGGTCGGGTGCATCGCCGCGGCGTCGGTCGTGGCCAAGGTG
ACCAGAGACCGGATCATGCGTAGTTTGCACAAGCGGTATCCCGAGTACGATTTCGCGCAGCACAAGGGCTACGTCACAGC
GGCGCACACCGCCGCGATGACGCGGTACGGGCCGTGCGAGCAGCACCGACTGTCATATGTCAACGTCGCCGCGCTGGCGG
CGCCGGCAGGGGAAACACGATCGTTGCGGCTGGAGGACAGGGTTGCTATGACCAGCCTGCGTGGCGTCACGGAGACCGCA
TGA

Upstream 100 bases:

>100_bases
GGTCCATCGGCTGGTCGACGCGGGCGAGCGGGGAGCGGCGCTGCGGCCCGCGCGGGCGGTGGGCGAGACGGCGCGGTATG
GGCGGTTCACCCTGATCGCG

Downstream 100 bases:

>100_bases
GCGCGGAGGATCTCGAAAAGTACGAGACCGAGATGGAGCTCCAGCTCTACCGCGAGTACCGCGACGTGGTCGGGTTGTTC
TCGTACGTGATCGAGACGGA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MDVRGTAIRSDAGLFGYERALVRRGLGPVAGVDEAGRGACAGPMVVAAVVLDQRRLGLLRRLADSKLLTERVREEVHADV
LAAAAAVSTIVIPAAEIDRNGVHVANIMGMRRAVAVLDVRPRYVLTDGFAVAGLGAESLAVIKGDLMVGCIAAASVVAKV
TRDRIMRSLHKRYPEYDFAQHKGYVTAAHTAAMTRYGPCEQHRLSYVNVAALAAPAGETRSLRLEDRVAMTSLRGVTETA

Sequences:

>Translated_240_residues
MDVRGTAIRSDAGLFGYERALVRRGLGPVAGVDEAGRGACAGPMVVAAVVLDQRRLGLLRRLADSKLLTERVREEVHADV
LAAAAAVSTIVIPAAEIDRNGVHVANIMGMRRAVAVLDVRPRYVLTDGFAVAGLGAESLAVIKGDLMVGCIAAASVVAKV
TRDRIMRSLHKRYPEYDFAQHKGYVTAAHTAAMTRYGPCEQHRLSYVNVAALAAPAGETRSLRLEDRVAMTSLRGVTETA
>Mature_240_residues
MDVRGTAIRSDAGLFGYERALVRRGLGPVAGVDEAGRGACAGPMVVAAVVLDQRRLGLLRRLADSKLLTERVREEVHADV
LAAAAAVSTIVIPAAEIDRNGVHVANIMGMRRAVAVLDVRPRYVLTDGFAVAGLGAESLAVIKGDLMVGCIAAASVVAKV
TRDRIMRSLHKRYPEYDFAQHKGYVTAAHTAAMTRYGPCEQHRLSYVNVAALAAPAGETRSLRLEDRVAMTSLRGVTETA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=178, Percent_Identity=43.8202247191011, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_FRASN (A8L6C6)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505514.1
- ProteinModelPortal:   A8L6C6
- SMR:   A8L6C6
- GeneID:   5669567
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_1154
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   FSP1855:FRANEAN1_1154-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25572; Mature: 25572

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRGTAIRSDAGLFGYERALVRRGLGPVAGVDEAGRGACAGPMVVAAVVLDQRRLGLLR
CCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLADSKLLTERVREEVHADVLAAAAAVSTIVIPAAEIDRNGVHVANIMGMRRAVAVLDVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCHHHHHHHHHHHHHEECC
PRYVLTDGFAVAGLGAESLAVIKGDLMVGCIAAASVVAKVTRDRIMRSLHKRYPEYDFAQ
CCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
HKGYVTAAHTAAMTRYGPCEQHRLSYVNVAALAAPAGETRSLRLEDRVAMTSLRGVTETA
CCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDVRGTAIRSDAGLFGYERALVRRGLGPVAGVDEAGRGACAGPMVVAAVVLDQRRLGLLR
CCCCCCEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RLADSKLLTERVREEVHADVLAAAAAVSTIVIPAAEIDRNGVHVANIMGMRRAVAVLDVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCCCHHHHHHHHHHHHHEECC
PRYVLTDGFAVAGLGAESLAVIKGDLMVGCIAAASVVAKVTRDRIMRSLHKRYPEYDFAQ
CCEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
HKGYVTAAHTAAMTRYGPCEQHRLSYVNVAALAAPAGETRSLRLEDRVAMTSLRGVTETA
CCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA