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The map label for this gene is ilvB1 [H]

Identifier: 158312944

GI number: 158312944

Start: 1301369

End: 1303165

Strand: Direct

Name: ilvB1 [H]

Synonym: Franean1_1090

Alternate gene names: 158312944

Gene position: 1301369-1303165 (Clockwise)

Preceding gene: 158312943

Following gene: 158312945

Centisome position: 14.49

GC content: 71.56

Gene sequence:

>1797_bases
ATGACCAGAGAGACCAGACAGATCACCGGAGCGCAGTCGCTCGTCCACTCGCTCGAGGCGGTCGGTGCCGACGTGGTCTT
CGGCATTCCGGGCGGGGCGATCCTGCCCGCCTACGATCCGCTGTTCGACTCCACCCGGGTCCGGCACGTGCTCGTCCGGC
ACGAGCAGGGCGCCGGGCACGCCGCCGAGGGGTATGCCCAGGCCACCGGGCGTGTCGGGGTCTGCATGGCGACGTCCGGG
CCGGGCGCGACGAACCTCGTGACACCGATCGCGGACGCCTACATGGACTCGGTGCCCATCGTCGCCATCACCGGGCAGGT
GCCGAGCGCGGCGATCGGCACCGACGCCTTCCAGGAAGCCGACATCTGCGGCATCACGCTGCCGATCACCAAGCACAACT
TCCTGGTGCAGTCGGCGGACGACATCCCACGCACGATCGCCGAGGCGTTCCACATCGCCTCGACGGGGCGCCCGGGCCCG
GTGCTGGTCGACCTGCCGAAGGACATCCTGCAGGCGGTCACGTCGGTCCTGCCGCACGAGGTGTGGCCGCCGACGCTCGA
CCTGCCCGGCTACCGCCCGGTCACCCGGCCGCACGCCAAGCAGGTCCGCGAGGCGGCCAAGATGATCAGTGCGGCGCGCC
GGCCGGTGCTCTACGTGGGCGGTGGGGTGCTGAAGGCCCGTGCCGCCGCCGAGCTGCGGGTGCTCGCCGAGCTCACCGGC
ATCCCGGTGGTCACCACGCTGATGGCGCGCGGCGCGTTCCCCGACTCCCACCCGCAGCACCTGGGCATGCCCGGGATGCA
CGGCTCCGTCGCCGCGGTCACCGCGATGCAGAAGGCCGACCTGCTGATCACCCTGGGGGCCAGGTTCGACGACCGGGTCA
CCGGCAAGCTGTCGTCATTCGCGCCCGGCGCGGCCGTCATCCACGCCGACATCGACCCGGCCGAGATCGGCAAGAACCGG
ATCGCCGACGTGCCGATCGTGGGCGACTGCCGGGAGGTCATCGTCGACCTGACCGCGGCACTGCGCACCGAGGAGCGCCC
CGACCTCGAGGGCTGGTGGCGGTCGCTGGACAGGTGGCGCGCGACCTACCCGCTCGGCTACGACCAGCCCGCCGACGGCT
CGCTCGCCCCGCAGCAGGTCATCGAGCGGATCGGCCGGATCGCCGGCCCGGAGACCGTTTTCGCGGCCGGGGTGGGGCAG
CACCAGATGTGGGCCGCCCAGTTCATCTCCTACGAGCACCCCTACACCTGGCTGAACTCCGGCGGCGCCGGCACCATGGG
CTACGCCGTGCCGGCCGCGATGGGCGCCAAGGTCGGCCGCCCGGAGGCCACCGTGTGGGCGATCGACGGCGACGGCTGCT
TCCAGATGACCAACCAGGAGCTCGCCACCTGCGCCCTGGAGGGCATCCCGATCAAGGTCGCGGTCATCAACAACGGTTCG
CTGGGCATGGTCCGGCAGTGGCAGACGCTCTTCTACGACAAGCGCTACTCGAACACCGACCTGGGCACCCACCCGGTGTC
GGCGCGCACCGGGGTGACCCGGGTCCCCGACTTCGTGCGCCTCGCCGAGGCCCTGGGCTGCGTCGGCCTGCGCTGTGAGT
CGCCCGCGGACGTGGACGCCACGATCGAGAAGGCGATGAGCATCGACGACGCCCCGGTCGTGGTCGACTTCGTGGTCCAC
CCCGACGCGATGGTGTGGCCGATGGTGGCCGCCGGCTCGAGCAACGACGAGATCCGCGTCGCCCGCGACATCGCCCCCGA
CTTCGACTACTCCGGCGACGCGGAGGTCAACCTGTGA

Upstream 100 bases:

>100_bases
TGGCAACACCGCGCACGGCGACCACCCGTCGCCGTCCGTGGACCCGTCCGTGAACGACGTCCCGTCCGCACCTCGCCGGC
CCCGTCAAGGAAGACGACAG

Downstream 100 bases:

>100_bases
CCCGGCACACTCTCTCGGTGCTCGTCGAGAACAAGCCCGGCGTCCTGGCCCGGGTGTCCGGCCTGTTCTCCCGGCGCGGC
TTCAACATCGAGTCGCTCGC

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTRETRQITGAQSLVHSLEAVGADVVFGIPGGAILPAYDPLFDSTRVRHVLVRHEQGAGHAAEGYAQATGRVGVCMATSG
PGATNLVTPIADAYMDSVPIVAITGQVPSAAIGTDAFQEADICGITLPITKHNFLVQSADDIPRTIAEAFHIASTGRPGP
VLVDLPKDILQAVTSVLPHEVWPPTLDLPGYRPVTRPHAKQVREAAKMISAARRPVLYVGGGVLKARAAAELRVLAELTG
IPVVTTLMARGAFPDSHPQHLGMPGMHGSVAAVTAMQKADLLITLGARFDDRVTGKLSSFAPGAAVIHADIDPAEIGKNR
IADVPIVGDCREVIVDLTAALRTEERPDLEGWWRSLDRWRATYPLGYDQPADGSLAPQQVIERIGRIAGPETVFAAGVGQ
HQMWAAQFISYEHPYTWLNSGGAGTMGYAVPAAMGAKVGRPEATVWAIDGDGCFQMTNQELATCALEGIPIKVAVINNGS
LGMVRQWQTLFYDKRYSNTDLGTHPVSARTGVTRVPDFVRLAEALGCVGLRCESPADVDATIEKAMSIDDAPVVVDFVVH
PDAMVWPMVAAGSSNDEIRVARDIAPDFDYSGDAEVNL

Sequences:

>Translated_598_residues
MTRETRQITGAQSLVHSLEAVGADVVFGIPGGAILPAYDPLFDSTRVRHVLVRHEQGAGHAAEGYAQATGRVGVCMATSG
PGATNLVTPIADAYMDSVPIVAITGQVPSAAIGTDAFQEADICGITLPITKHNFLVQSADDIPRTIAEAFHIASTGRPGP
VLVDLPKDILQAVTSVLPHEVWPPTLDLPGYRPVTRPHAKQVREAAKMISAARRPVLYVGGGVLKARAAAELRVLAELTG
IPVVTTLMARGAFPDSHPQHLGMPGMHGSVAAVTAMQKADLLITLGARFDDRVTGKLSSFAPGAAVIHADIDPAEIGKNR
IADVPIVGDCREVIVDLTAALRTEERPDLEGWWRSLDRWRATYPLGYDQPADGSLAPQQVIERIGRIAGPETVFAAGVGQ
HQMWAAQFISYEHPYTWLNSGGAGTMGYAVPAAMGAKVGRPEATVWAIDGDGCFQMTNQELATCALEGIPIKVAVINNGS
LGMVRQWQTLFYDKRYSNTDLGTHPVSARTGVTRVPDFVRLAEALGCVGLRCESPADVDATIEKAMSIDDAPVVVDFVVH
PDAMVWPMVAAGSSNDEIRVARDIAPDFDYSGDAEVNL
>Mature_597_residues
TRETRQITGAQSLVHSLEAVGADVVFGIPGGAILPAYDPLFDSTRVRHVLVRHEQGAGHAAEGYAQATGRVGVCMATSGP
GATNLVTPIADAYMDSVPIVAITGQVPSAAIGTDAFQEADICGITLPITKHNFLVQSADDIPRTIAEAFHIASTGRPGPV
LVDLPKDILQAVTSVLPHEVWPPTLDLPGYRPVTRPHAKQVREAAKMISAARRPVLYVGGGVLKARAAAELRVLAELTGI
PVVTTLMARGAFPDSHPQHLGMPGMHGSVAAVTAMQKADLLITLGARFDDRVTGKLSSFAPGAAVIHADIDPAEIGKNRI
ADVPIVGDCREVIVDLTAALRTEERPDLEGWWRSLDRWRATYPLGYDQPADGSLAPQQVIERIGRIAGPETVFAAGVGQH
QMWAAQFISYEHPYTWLNSGGAGTMGYAVPAAMGAKVGRPEATVWAIDGDGCFQMTNQELATCALEGIPIKVAVINNGSL
GMVRQWQTLFYDKRYSNTDLGTHPVSARTGVTRVPDFVRLAEALGCVGLRCESPADVDATIEKAMSIDDAPVVVDFVVHP
DAMVWPMVAAGSSNDEIRVARDIAPDFDYSGDAEVNL

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=575, Percent_Identity=24.1739130434783, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI21361361, Length=514, Percent_Identity=26.4591439688716, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI87081685, Length=581, Percent_Identity=44.0619621342513, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1790104, Length=577, Percent_Identity=41.7677642980936, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=591, Percent_Identity=35.0253807106599, Blast_Score=329, Evalue=4e-91,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=29.2035398230088, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788716, Length=496, Percent_Identity=25.8064516129032, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=581, Percent_Identity=25.9896729776248, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=581, Percent_Identity=25.8175559380379, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=514, Percent_Identity=25.2918287937743, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=43.6332767402377, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=23.3606557377049, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=498, Percent_Identity=24.4979919678715, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=495, Percent_Identity=23.2323232323232, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=23.2464929859719, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=578, Percent_Identity=26.643598615917, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63565; Mature: 63434

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00187 TPP_ENZYMES ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRETRQITGAQSLVHSLEAVGADVVFGIPGGAILPAYDPLFDSTRVRHVLVRHEQGAGH
CCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCCCC
AAEGYAQATGRVGVCMATSGPGATNLVTPIADAYMDSVPIVAITGQVPSAAIGTDAFQEA
CCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHCCC
DICGITLPITKHNFLVQSADDIPRTIAEAFHIASTGRPGPVLVDLPKDILQAVTSVLPHE
CCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCC
VWPPTLDLPGYRPVTRPHAKQVREAAKMISAARRPVLYVGGGVLKARAAAELRVLAELTG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCC
IPVVTTLMARGAFPDSHPQHLGMPGMHGSVAAVTAMQKADLLITLGARFDDRVTGKLSSF
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCHHCCHHCC
APGAAVIHADIDPAEIGKNRIADVPIVGDCREVIVDLTAALRTEERPDLEGWWRSLDRWR
CCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ATYPLGYDQPADGSLAPQQVIERIGRIAGPETVFAAGVGQHQMWAAQFISYEHPYTWLNS
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEEEECC
GGAGTMGYAVPAAMGAKVGRPEATVWAIDGDGCFQMTNQELATCALEGIPIKVAVINNGS
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHCCCEEEEEEEECCC
LGMVRQWQTLFYDKRYSNTDLGTHPVSARTGVTRVPDFVRLAEALGCVGLRCESPADVDA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCHH
TIEKAMSIDDAPVVVDFVVHPDAMVWPMVAAGSSNDEIRVARDIAPDFDYSGDAEVNL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRETRQITGAQSLVHSLEAVGADVVFGIPGGAILPAYDPLFDSTRVRHVLVRHEQGAGH
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHEEEEEECCCCCC
AAEGYAQATGRVGVCMATSGPGATNLVTPIADAYMDSVPIVAITGQVPSAAIGTDAFQEA
CCCCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHCCC
DICGITLPITKHNFLVQSADDIPRTIAEAFHIASTGRPGPVLVDLPKDILQAVTSVLPHE
CCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHCCCC
VWPPTLDLPGYRPVTRPHAKQVREAAKMISAARRPVLYVGGGVLKARAAAELRVLAELTG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCC
IPVVTTLMARGAFPDSHPQHLGMPGMHGSVAAVTAMQKADLLITLGARFDDRVTGKLSSF
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCHHCCHHCC
APGAAVIHADIDPAEIGKNRIADVPIVGDCREVIVDLTAALRTEERPDLEGWWRSLDRWR
CCCCEEEEECCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ATYPLGYDQPADGSLAPQQVIERIGRIAGPETVFAAGVGQHQMWAAQFISYEHPYTWLNS
EECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCEEEECC
GGAGTMGYAVPAAMGAKVGRPEATVWAIDGDGCFQMTNQELATCALEGIPIKVAVINNGS
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEECCHHHHHHHHCCCEEEEEEEECCC
LGMVRQWQTLFYDKRYSNTDLGTHPVSARTGVTRVPDFVRLAEALGCVGLRCESPADVDA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCHH
TIEKAMSIDDAPVVVDFVVHPDAMVWPMVAAGSSNDEIRVARDIAPDFDYSGDAEVNL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]