Click here to switch to the map view.

The map label for this gene is pckG

Identifier: 158312689

GI number: 158312689

Start: 974309

End: 976132

Strand: Direct

Name: pckG

Synonym: Franean1_0833

Alternate gene names: 158312689

Gene position: 974309-976132 (Clockwise)

Preceding gene: 158312687

Following gene: 158312690

Centisome position: 10.85

GC content: 68.15

Gene sequence:

>1824_bases
GTGACGACCGCGGCACAGATTCCCGGGCTGGATGCCGCCCCCACCAAGCACGCCAGGCTGCTCGCCTGGGTGCGTGAGAT
CGCCGAACTCACCCAGCCGGACCGTATTGAATGGTGCGACGGCTCGGAATCCGAGTTCGATCGGCTGACATCACTGCTCA
TCGACAAGGGCACTCTCGTCCAGCTTGACGACGAGAAACGCCCGAACAGCTTCTACGCCGCTTCGGACCCCAGCGACGTG
GCGCGTGTGGAGGACCGGACCTACATTTGTTCGGAAAAGGCCGAGGACGCCGGCCCGACGAACAACTGGCTTGATCCGGC
CGAAATGCGCACGAAGCTGGGTAAGCTCTTCTCCGGATGCATGCGGGGACGGACGATGTATGTCGTCCCGTTCTGCATGG
GTCCGCTCGGTTCGCACATCTCGGCGCTCGGCGTCGAAATCACGGACTCGCCCTACGTCGTCATCTCTATGCGCACGATG
ACCCGTATGGGGACTCCCGCGCTGGACCAGCTCGGTGACGACGGCTTCTTCGTCCCGGCGGTTCATTCACTGGGCGCGCC
CCTGGAGCCGGGCGCGACGGACGTCCCGTGGCCGTGTAATTCGACGAAGTACATCACGCACTTCCCGGAGACCCGCGAGA
TCTGGTCGTACGGCTCCGGTTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTACGCCCTGCGCATCGCCTCGGTCATG
GCGCGTGACCAGGGGTGGCTGGCCGAGCACATGCTGATCCTCAAGCTGACCTCGCCGGCCGGCAAGGTCCACTTCATCGC
CGCCGCGTTCCCGTCGGCATGCGGCAAGACCAACCTCGCGATGCTGATCCCGACGCTGCCCGGCTGGAAGGCCGAGACGG
TCGGCGACGACATCGCCTGGATGCGCTTCGGTGACGACGGCCGGCTCTACGCCGTCAACCCGGAGGCGGGTCTGTTCGGG
GTGGCTCCGGGCACCGGCGAGGAGACCAATCCGAACGCGGTCAAGACCCTGTGGGGGAACGCGGTCTTCACCAACGTCGC
GCGGACCGACGACGGTGACGTCTGGTGGGAGGGCCTCACTCCGGACAAGCCCGCACACCTCATCGACTGGAAGGGCCGGG
ACTGGACGCCCGACTCGACCGAACCGGCCGCGCACCCGAACGCCCGGTTCACCGTGCCGGCGAGCCAGTGCCCGACCATG
GCGCCCGAGTGGGAGAACCCGGCCGGCGTTCCCATCTCGGCGATCCTGTTCGGCGGCCGCCGCGCGACGGCCGTCCCGCT
GGTGACCGAGGCGCCGGACTGGCAGCGCGGGGTGTTCTTCGGCTCGATCGTCTCCTCCGAGACCACTGCCGCCGCGGCCG
GGGCGATCGGCAAGCTGCGACGGGACCCGTTCGCGATGCTGCCGTTCTGCGGCTACAACATGGCCGACTATTTCGCCCAC
TGGCTGGAGATCGGCAACAAGGCCGACCCGGACAAGCTGCCGCGGATCTACTACGTGAACTGGTTCCGCAAGAGCCCCGA
GGGCCGTTACCTCTGGCCAGGGTTCGGCGAGAACAGCCGCGTGCTGGCCTGGGTCGCGAGCCGGCTCGACGGCGAGGCGA
AGGGCACGGAGACCCCGCTGGGCATCCTCCCGGCCCCGGGCGAGCTCCCCACCGAGGGCCTGGACGTCTCCGAGGCCGAC
CTGGAGACGCTGCTCTCCGTCGACACCGAGGTGTGGCGGCAGGAGGCGGAGCTGATCCCGGAGCACTACAACACCTTCGG
CGACCGCCTGCCGGCCCAGCTGTGGGCGGAGCACGAGGCCCTCACCAAGCGCCTCAACGGCTGA

Upstream 100 bases:

>100_bases
GGTGTCTGGTGGTGTTCGGGTGCGAACGCGGCACCTGGCTCACGGCACTCGCACACGGGACGAGAACGCGACAGGTTCAA
CTCGACCGGAGGTGTGAGCT

Downstream 100 bases:

>100_bases
CGGCCAGCCAGCCAGCTCGCGCGGGCTGAGCTCGGCGCGGGCCGGGTTTCTCGAGGGCTGAACGGCCTCGAGGGCCAGGC
GTCTGGACGGCTGACGACCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MTTAAQIPGLDAAPTKHARLLAWVREIAELTQPDRIEWCDGSESEFDRLTSLLIDKGTLVQLDDEKRPNSFYAASDPSDV
ARVEDRTYICSEKAEDAGPTNNWLDPAEMRTKLGKLFSGCMRGRTMYVVPFCMGPLGSHISALGVEITDSPYVVISMRTM
TRMGTPALDQLGDDGFFVPAVHSLGAPLEPGATDVPWPCNSTKYITHFPETREIWSYGSGYGGNALLGKKCYALRIASVM
ARDQGWLAEHMLILKLTSPAGKVHFIAAAFPSACGKTNLAMLIPTLPGWKAETVGDDIAWMRFGDDGRLYAVNPEAGLFG
VAPGTGEETNPNAVKTLWGNAVFTNVARTDDGDVWWEGLTPDKPAHLIDWKGRDWTPDSTEPAAHPNARFTVPASQCPTM
APEWENPAGVPISAILFGGRRATAVPLVTEAPDWQRGVFFGSIVSSETTAAAAGAIGKLRRDPFAMLPFCGYNMADYFAH
WLEIGNKADPDKLPRIYYVNWFRKSPEGRYLWPGFGENSRVLAWVASRLDGEAKGTETPLGILPAPGELPTEGLDVSEAD
LETLLSVDTEVWRQEAELIPEHYNTFGDRLPAQLWAEHEALTKRLNG

Sequences:

>Translated_607_residues
MTTAAQIPGLDAAPTKHARLLAWVREIAELTQPDRIEWCDGSESEFDRLTSLLIDKGTLVQLDDEKRPNSFYAASDPSDV
ARVEDRTYICSEKAEDAGPTNNWLDPAEMRTKLGKLFSGCMRGRTMYVVPFCMGPLGSHISALGVEITDSPYVVISMRTM
TRMGTPALDQLGDDGFFVPAVHSLGAPLEPGATDVPWPCNSTKYITHFPETREIWSYGSGYGGNALLGKKCYALRIASVM
ARDQGWLAEHMLILKLTSPAGKVHFIAAAFPSACGKTNLAMLIPTLPGWKAETVGDDIAWMRFGDDGRLYAVNPEAGLFG
VAPGTGEETNPNAVKTLWGNAVFTNVARTDDGDVWWEGLTPDKPAHLIDWKGRDWTPDSTEPAAHPNARFTVPASQCPTM
APEWENPAGVPISAILFGGRRATAVPLVTEAPDWQRGVFFGSIVSSETTAAAAGAIGKLRRDPFAMLPFCGYNMADYFAH
WLEIGNKADPDKLPRIYYVNWFRKSPEGRYLWPGFGENSRVLAWVASRLDGEAKGTETPLGILPAPGELPTEGLDVSEAD
LETLLSVDTEVWRQEAELIPEHYNTFGDRLPAQLWAEHEALTKRLNG
>Mature_606_residues
TTAAQIPGLDAAPTKHARLLAWVREIAELTQPDRIEWCDGSESEFDRLTSLLIDKGTLVQLDDEKRPNSFYAASDPSDVA
RVEDRTYICSEKAEDAGPTNNWLDPAEMRTKLGKLFSGCMRGRTMYVVPFCMGPLGSHISALGVEITDSPYVVISMRTMT
RMGTPALDQLGDDGFFVPAVHSLGAPLEPGATDVPWPCNSTKYITHFPETREIWSYGSGYGGNALLGKKCYALRIASVMA
RDQGWLAEHMLILKLTSPAGKVHFIAAAFPSACGKTNLAMLIPTLPGWKAETVGDDIAWMRFGDDGRLYAVNPEAGLFGV
APGTGEETNPNAVKTLWGNAVFTNVARTDDGDVWWEGLTPDKPAHLIDWKGRDWTPDSTEPAAHPNARFTVPASQCPTMA
PEWENPAGVPISAILFGGRRATAVPLVTEAPDWQRGVFFGSIVSSETTAAAAGAIGKLRRDPFAMLPFCGYNMADYFAHW
LEIGNKADPDKLPRIYYVNWFRKSPEGRYLWPGFGENSRVLAWVASRLDGEAKGTETPLGILPAPGELPTEGLDVSEADL
ETLLSVDTEVWRQEAELIPEHYNTFGDRLPAQLWAEHEALTKRLNG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=598, Percent_Identity=52.5083612040134, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI187281517, Length=604, Percent_Identity=50.9933774834437, Blast_Score=601, Evalue=1e-172,
Organism=Homo sapiens, GI66346723, Length=368, Percent_Identity=55.1630434782609, Blast_Score=391, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989645, Length=606, Percent_Identity=50.6600660066007, Blast_Score=597, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71989658, Length=606, Percent_Identity=50.6600660066007, Blast_Score=596, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI71989653, Length=592, Percent_Identity=51.0135135135135, Blast_Score=587, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17555492, Length=615, Percent_Identity=47.6422764227642, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=537, Percent_Identity=50.8379888268156, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI32564407, Length=523, Percent_Identity=50.6692160611855, Blast_Score=515, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71989649, Length=509, Percent_Identity=51.2770137524558, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32564409, Length=368, Percent_Identity=47.8260869565217, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17540956, Length=530, Percent_Identity=25.6603773584906, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24655085, Length=600, Percent_Identity=50.8333333333333, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24655082, Length=598, Percent_Identity=50.8361204013378, Blast_Score=600, Evalue=1e-171,
Organism=Drosophila melanogaster, GI161077226, Length=568, Percent_Identity=51.4084507042254, Blast_Score=572, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24655088, Length=504, Percent_Identity=52.5793650793651, Blast_Score=538, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_FRASN (A8L175)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001505197.1
- ProteinModelPortal:   A8L175
- SMR:   A8L175
- GeneID:   5669249
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_0833
- HOGENOM:   HBG484437
- OMA:   EGLGDVN
- ProtClustDB:   PRK04210
- BioCyc:   FSP1855:FRANEAN1_0833-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 66459; Mature: 66327

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 274-274 BINDING 82-82 BINDING 223-223 BINDING 230-230 BINDING 272-272 BINDING 389-389 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAQIPGLDAAPTKHARLLAWVREIAELTQPDRIEWCDGSESEFDRLTSLLIDKGTLV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCEE
QLDDEKRPNSFYAASDPSDVARVEDRTYICSEKAEDAGPTNNWLDPAEMRTKLGKLFSGC
EECCCCCCCCEEECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MRGRTMYVVPFCMGPLGSHISALGVEITDSPYVVISMRTMTRMGTPALDQLGDDGFFVPA
HCCCEEEEEEEHHCCHHHHHHHEEEEECCCCEEEEEEHHHHHCCCCHHHHCCCCCEEHHH
VHSLGAPLEPGATDVPWPCNSTKYITHFPETREIWSYGSGYGGNALLGKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARDQGWLAEHMLILKLTSPAGKVHFIAAAFPSACGKTNLAMLIPTLPGWKAETVGDDIAW
HHCCCHHHHEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEEECCCCCCCCCCCCCEEE
MRFGDDGRLYAVNPEAGLFGVAPGTGEETNPNAVKTLWGNAVFTNVARTDDGDVWWEGLT
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCHHHHHHCCHHEEECCCCCCCCEEECCCC
PDKPAHLIDWKGRDWTPDSTEPAAHPNARFTVPASQCPTMAPEWENPAGVPISAILFGGR
CCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEECCC
RATAVPLVTEAPDWQRGVFFGSIVSSETTAAAAGAIGKLRRDPFAMLPFCGYNMADYFAH
CCEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHH
WLEIGNKADPDKLPRIYYVNWFRKSPEGRYLWPGFGENSRVLAWVASRLDGEAKGTETPL
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCCEEHHHHHHHCCCCCCCCCCCC
GILPAPGELPTEGLDVSEADLETLLSVDTEVWRQEAELIPEHYNTFGDRLPAQLWAEHEA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHH
LTKRLNG
HHHHCCC
>Mature Secondary Structure 
TTAAQIPGLDAAPTKHARLLAWVREIAELTQPDRIEWCDGSESEFDRLTSLLIDKGTLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCEE
QLDDEKRPNSFYAASDPSDVARVEDRTYICSEKAEDAGPTNNWLDPAEMRTKLGKLFSGC
EECCCCCCCCEEECCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
MRGRTMYVVPFCMGPLGSHISALGVEITDSPYVVISMRTMTRMGTPALDQLGDDGFFVPA
HCCCEEEEEEEHHCCHHHHHHHEEEEECCCCEEEEEEHHHHHCCCCHHHHCCCCCEEHHH
VHSLGAPLEPGATDVPWPCNSTKYITHFPETREIWSYGSGYGGNALLGKKCYALRIASVM
HHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARDQGWLAEHMLILKLTSPAGKVHFIAAAFPSACGKTNLAMLIPTLPGWKAETVGDDIAW
HHCCCHHHHEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEEECCCCCCCCCCCCCEEE
MRFGDDGRLYAVNPEAGLFGVAPGTGEETNPNAVKTLWGNAVFTNVARTDDGDVWWEGLT
EEECCCCEEEEECCCCCEEECCCCCCCCCCCCHHHHHHCCHHEEECCCCCCCCEEECCCC
PDKPAHLIDWKGRDWTPDSTEPAAHPNARFTVPASQCPTMAPEWENPAGVPISAILFGGR
CCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEECCC
RATAVPLVTEAPDWQRGVFFGSIVSSETTAAAAGAIGKLRRDPFAMLPFCGYNMADYFAH
CCEEEEEEECCCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHH
WLEIGNKADPDKLPRIYYVNWFRKSPEGRYLWPGFGENSRVLAWVASRLDGEAKGTETPL
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCCEEHHHHHHHCCCCCCCCCCCC
GILPAPGELPTEGLDVSEADLETLLSVDTEVWRQEAELIPEHYNTFGDRLPAQLWAEHEA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHH
LTKRLNG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA