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The map label for this gene is wcaF [H]

Identifier: 158312614

GI number: 158312614

Start: 885304

End: 885933

Strand: Direct

Name: wcaF [H]

Synonym: Franean1_0758

Alternate gene names: 158312614

Gene position: 885304-885933 (Clockwise)

Preceding gene: 158312613

Following gene: 158312615

Centisome position: 9.86

GC content: 67.94

Gene sequence:

>630_bases
GTGACCAGGGTTCTCGGCTTTCTTCGTTCCCTGCTCGACGCCCGCGCCCTCCTGCACTCACTGCGGATCCTCCACTTCTA
CAACTACTCGCATGTCCAGCAGGTTCGCCGGATGACGATCGAACGGGGTGTCCGCCTGGCGCCGAACGTCTCCGTCCGGA
ACGGGGAGCGGGTGGAGATACGGGCGTTCGCCCACATCGGTGAGCGGGTTTCGCTGTGGGCCGGTGACCGCACTGGCCGG
ATCGAGATCGGCGAGCACTCCCTGATCGGCCCGGGCACGTTTCTCACGGCCGCGAACTACGAGACGCTGCCGGACATCGT
CATCGACAGCCAGCCGAAGCGGGAGGCGGACATCGTCATCGGTAACGACGTGTGGGTCGGCGCCAACTCGGTGGTTCTTC
CGGGCGTGACGATAGGCGACAGCACGATTGTCGGCGCCGGTTCTGTCGTCACGAAGTCGTTGCCCGCCGGAGCGTTGGCC
GCGGGTGTTCCGGCGAAGGTCATCGGGAAGCGCGGGGAGTCGACCCGTTCCGGTCGTGCCGTCCAGGCGGACCGGCCTGA
GCAGAACGGCCGTACCGGTGCGGACGGCACCGGGCGGCGTCCCGGCTCCCACCCGGCGGGTGAGCAGTGA

Upstream 100 bases:

>100_bases
CGGCGGTGGCGAAGCTGCTGGCCATCCGGAAGGTCCGCCACGGCGACCTCGGTGCCTGGGAGCGTGACGACAGCAGCAGG
CGCCTTCTTGAGGAGACGAC

Downstream 100 bases:

>100_bases
CCGTCGAGGTGTCCGTGCTGGTCGTGTCCTACAACACCGCCGAGCTGACGGTCCGGTGCCTGGAGTCCGTCCTGGCTGAG
CCGGCCGGGGCCGAGATTGA

Product: acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTRVLGFLRSLLDARALLHSLRILHFYNYSHVQQVRRMTIERGVRLAPNVSVRNGERVEIRAFAHIGERVSLWAGDRTGR
IEIGEHSLIGPGTFLTAANYETLPDIVIDSQPKREADIVIGNDVWVGANSVVLPGVTIGDSTIVGAGSVVTKSLPAGALA
AGVPAKVIGKRGESTRSGRAVQADRPEQNGRTGADGTGRRPGSHPAGEQ

Sequences:

>Translated_209_residues
MTRVLGFLRSLLDARALLHSLRILHFYNYSHVQQVRRMTIERGVRLAPNVSVRNGERVEIRAFAHIGERVSLWAGDRTGR
IEIGEHSLIGPGTFLTAANYETLPDIVIDSQPKREADIVIGNDVWVGANSVVLPGVTIGDSTIVGAGSVVTKSLPAGALA
AGVPAKVIGKRGESTRSGRAVQADRPEQNGRTGADGTGRRPGSHPAGEQ
>Mature_208_residues
TRVLGFLRSLLDARALLHSLRILHFYNYSHVQQVRRMTIERGVRLAPNVSVRNGERVEIRAFAHIGERVSLWAGDRTGRI
EIGEHSLIGPGTFLTAANYETLPDIVIDSQPKREADIVIGNDVWVGANSVVLPGVTIGDSTIVGAGSVVTKSLPAGALAA
GVPAKVIGKRGESTRSGRAVQADRPEQNGRTGADGTGRRPGSHPAGEQ

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=136, Percent_Identity=36.0294117647059, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786537, Length=155, Percent_Identity=30.3225806451613, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788345, Length=190, Percent_Identity=30, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786664, Length=90, Percent_Identity=37.7777777777778, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 22312; Mature: 22180

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLGFLRSLLDARALLHSLRILHFYNYSHVQQVRRMTIERGVRLAPNVSVRNGERVEI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCEECCCCEEEE
RAFAHIGERVSLWAGDRTGRIEIGEHSLIGPGTFLTAANYETLPDIVIDSQPKREADIVI
EEEECCCCEEEEEECCCCCEEEECCCCEECCCCEEEECCCCCCCCEEECCCCCCCCCEEE
GNDVWVGANSVVLPGVTIGDSTIVGAGSVVTKSLPAGALAAGVPAKVIGKRGESTRSGRA
CCCEEECCCCEEECCEEECCCEEECCCHHHHHCCCCCHHHHCCCHHHHCCCCCCCCCCCE
VQADRPEQNGRTGADGTGRRPGSHPAGEQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRVLGFLRSLLDARALLHSLRILHFYNYSHVQQVRRMTIERGVRLAPNVSVRNGERVEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEECCCCEECCCCEEEE
RAFAHIGERVSLWAGDRTGRIEIGEHSLIGPGTFLTAANYETLPDIVIDSQPKREADIVI
EEEECCCCEEEEEECCCCCEEEECCCCEECCCCEEEECCCCCCCCEEECCCCCCCCCEEE
GNDVWVGANSVVLPGVTIGDSTIVGAGSVVTKSLPAGALAAGVPAKVIGKRGESTRSGRA
CCCEEECCCCEEECCEEECCCEEECCCHHHHHCCCCCHHHHCCCHHHHCCCCCCCCCCCE
VQADRPEQNGRTGADGTGRRPGSHPAGEQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]