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The map label for this gene is pleD [H]

Identifier: 158312524

GI number: 158312524

Start: 788004

End: 788648

Strand: Direct

Name: pleD [H]

Synonym: Franean1_0667

Alternate gene names: 158312524

Gene position: 788004-788648 (Clockwise)

Preceding gene: 158312523

Following gene: 158312525

Centisome position: 8.77

GC content: 70.08

Gene sequence:

>645_bases
GTGACCGAGCGGACCGGCGGTCCTGTCGACACCGAGGGCGCGTCGAACACTGAGCGGCCCCGGAGCAAGATCCTCCTGGT
GGACGACCGACCCGACAACCTGATGGCGCTGGAGGCGATCCTCGCCTCCCTGGACCAGGATCTGGTGACCGCGTCCTCGG
GCGAGGAGGCGCTCAAGCGCCTGCTCATCGACGACTTCGCGGTCATCCTCCTCGACGTCCAGATGCCTGGAATGGACGGC
TTCGAGACCGCGCACCGGATCAAGCAGCGCGGGCGGACGCGGGACACTCCGATCATCTTCCTGACGGCGATCGACCGCGA
GCCGCACCACGCCTTCCGGGGCTACGCGGTCGGCGCGGTGGACTACATCGCCAAGCCGTTCGACCCCTGGCTGCTGCGGG
CCAAGGTGAGCGTCTTCGTCGAGCTGTTCGAGAAGAACGAGCGGCTGGCCGAGCTCGCCCACCAGCGGGTGCGCGACTTC
GACCTCATCACCGAGGTCGCCGAGCGCCTGATCGCGCTTGACGTGCTCCTCGACGGCGCCGCGACGTCGAACGAGGAGAC
GCTCGCCGCCGCGAGCGCCGAGGTGCGCGGCCTGGTCTCGCGGCTGCGGCCCTTTGCGCGCTCGACGGGCGGCCAGCCCG
AGTAG

Upstream 100 bases:

>100_bases
ACGGGCCGGTGCTGGACCGGACATGCCGATCGGCGATGGTTGAGCAGTGGTGTCATCACCACGAACACCCAGGCCGACCG
GCGAAGGAGGAGGCAGGCGG

Downstream 100 bases:

>100_bases
CCGCTGCCGCTGCCGGCGCCGGCCGGCGCCGGCAGCGGCGGGGCCGGTAGTTGCCCGCCGACTGGCGGGTTGCCGTTTCG
TCCCCCTGCCGTGTGCGCCG

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTERTGGPVDTEGASNTERPRSKILLVDDRPDNLMALEAILASLDQDLVTASSGEEALKRLLIDDFAVILLDVQMPGMDG
FETAHRIKQRGRTRDTPIIFLTAIDREPHHAFRGYAVGAVDYIAKPFDPWLLRAKVSVFVELFEKNERLAELAHQRVRDF
DLITEVAERLIALDVLLDGAATSNEETLAAASAEVRGLVSRLRPFARSTGGQPE

Sequences:

>Translated_214_residues
MTERTGGPVDTEGASNTERPRSKILLVDDRPDNLMALEAILASLDQDLVTASSGEEALKRLLIDDFAVILLDVQMPGMDG
FETAHRIKQRGRTRDTPIIFLTAIDREPHHAFRGYAVGAVDYIAKPFDPWLLRAKVSVFVELFEKNERLAELAHQRVRDF
DLITEVAERLIALDVLLDGAATSNEETLAAASAEVRGLVSRLRPFARSTGGQPE
>Mature_213_residues
TERTGGPVDTEGASNTERPRSKILLVDDRPDNLMALEAILASLDQDLVTASSGEEALKRLLIDDFAVILLDVQMPGMDGF
ETAHRIKQRGRTRDTPIIFLTAIDREPHHAFRGYAVGAVDYIAKPFDPWLLRAKVSVFVELFEKNERLAELAHQRVRDFD
LITEVAERLIALDVLLDGAATSNEETLAAASAEVRGLVSRLRPFARSTGGQPE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=35.4545454545455, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 23663; Mature: 23532

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERTGGPVDTEGASNTERPRSKILLVDDRPDNLMALEAILASLDQDLVTASSGEEALKR
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLIDDFAVILLDVQMPGMDGFETAHRIKQRGRTRDTPIIFLTAIDREPHHAFRGYAVGAV
HHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHCHHHHHH
DYIAKPFDPWLLRAKVSVFVELFEKNERLAELAHQRVRDFDLITEVAERLIALDVLLDGA
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ATSNEETLAAASAEVRGLVSRLRPFARSTGGQPE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TERTGGPVDTEGASNTERPRSKILLVDDRPDNLMALEAILASLDQDLVTASSGEEALKR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
LLIDDFAVILLDVQMPGMDGFETAHRIKQRGRTRDTPIIFLTAIDREPHHAFRGYAVGAV
HHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHCHHHHHH
DYIAKPFDPWLLRAKVSVFVELFEKNERLAELAHQRVRDFDLITEVAERLIALDVLLDGA
HHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ATSNEETLAAASAEVRGLVSRLRPFARSTGGQPE
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA