Click here to switch to the map view.

The map label for this gene is yjjN [H]

Identifier: 158312443

GI number: 158312443

Start: 678635

End: 679573

Strand: Direct

Name: yjjN [H]

Synonym: Franean1_0586

Alternate gene names: 158312443

Gene position: 678635-679573 (Clockwise)

Preceding gene: 158312442

Following gene: 158312444

Centisome position: 7.56

GC content: 71.78

Gene sequence:

>939_bases
GTGAAGGCCGCACGCCCCAAAGCGGGAGAGATCACCGTCGTCGACCTCGACGAGCCACCGGGAGTCGGGGACGAGGTCAT
CCGGATCGCGGCAGCGAGCATCTGCAGCTCGGACCTGATGTACATGCGGTTCGGGCTGGAACGGGTCATCGGACATGAGC
TGGCCGGCGTCCGCGCGGACGGAACCGGGGCCGTCGTGGAGGCGCTCTACGGGTGCATGGAATGCGACCTGTGTCTGGAC
GGCCGGTACAACCTGTGCCCCACCCACATCCATCGGGCACTGGGCTCCAGCGCGGACGGCGGGATGGCCGAGCAGTTCCG
CGTCCCCTCGCAGCGGCTCGTCGCGCTGCCGCCCGGTCTCGATATCCGCGACGCCTCGCTCGTCGAGCCCGCGTCGGTCT
CCTGGCACGCCCTCCGGCTGGCCGGCACCGGTCCGGACACCCGGCTGGCTGTCGTGGGGGCCGGTGCACTCGGCCTGCTC
GCCGCCGCCGGCGCGCGCCGTCAGGGTGCTCCCGAGGTGGACCTCGAGGCACGCCATCCGCACCAGGTCGAAGCCGGCGA
GCGCCTCGGGGCCAAGGTCGGGACCACGGGCCTGTACGACGTGGTCGTGGAGGCCGCGGGAACGTCGGCCAGCCTCGCCC
GCGCCGTCGACCTCGTGGCGCCGGGCGGGACGGTGGTCGTCGTCGGCTTCCACATGGGCACGGTGGAAGTCAACTGGTCT
CCGCTGTTCCACCGTGAGGCCCGGCTCATCCCCTCGATGAGCTACTGCCGGCACGCGAACGGGCGCGAGATGGAGGACGC
GGCGGCCATGCTCGCCGACAACCCGGAGATCGCCCGCACGCTGATCACCCATCGGTTCCCGCTTGAGGACGCTGTCGAGG
CGTTCCGGGTAGCCGCCGACCGCAAGGCCGGAGCGCTGCGGGTGGTGGTCGAACCATGA

Upstream 100 bases:

>100_bases
TCGACGCCATCGACAAGATCGTGCCTCCCGGCACCGACATGCCCGGGATCGACCACTTCACCCAGCACCCGGCCCTCCTG
CCCGCCGCCCGCCGACGCAC

Downstream 100 bases:

>100_bases
CCTCCGTGGGCATCGTGACGGGCGCCGGCCCTACAGGGGCGTGACCGCACCACCGGCGACGAAGCCGCCAAGGGATATGG
CCGTCTCACTCTGAGCGCCA

Product: alcohol dehydrogenase

Products: L-sorbose; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKAARPKAGEITVVDLDEPPGVGDEVIRIAAASICSSDLMYMRFGLERVIGHELAGVRADGTGAVVEALYGCMECDLCLD
GRYNLCPTHIHRALGSSADGGMAEQFRVPSQRLVALPPGLDIRDASLVEPASVSWHALRLAGTGPDTRLAVVGAGALGLL
AAAGARRQGAPEVDLEARHPHQVEAGERLGAKVGTTGLYDVVVEAAGTSASLARAVDLVAPGGTVVVVGFHMGTVEVNWS
PLFHREARLIPSMSYCRHANGREMEDAAAMLADNPEIARTLITHRFPLEDAVEAFRVAADRKAGALRVVVEP

Sequences:

>Translated_312_residues
MKAARPKAGEITVVDLDEPPGVGDEVIRIAAASICSSDLMYMRFGLERVIGHELAGVRADGTGAVVEALYGCMECDLCLD
GRYNLCPTHIHRALGSSADGGMAEQFRVPSQRLVALPPGLDIRDASLVEPASVSWHALRLAGTGPDTRLAVVGAGALGLL
AAAGARRQGAPEVDLEARHPHQVEAGERLGAKVGTTGLYDVVVEAAGTSASLARAVDLVAPGGTVVVVGFHMGTVEVNWS
PLFHREARLIPSMSYCRHANGREMEDAAAMLADNPEIARTLITHRFPLEDAVEAFRVAADRKAGALRVVVEP
>Mature_312_residues
MKAARPKAGEITVVDLDEPPGVGDEVIRIAAASICSSDLMYMRFGLERVIGHELAGVRADGTGAVVEALYGCMECDLCLD
GRYNLCPTHIHRALGSSADGGMAEQFRVPSQRLVALPPGLDIRDASLVEPASVSWHALRLAGTGPDTRLAVVGAGALGLL
AAAGARRQGAPEVDLEARHPHQVEAGERLGAKVGTTGLYDVVVEAAGTSASLARAVDLVAPGGTVVVVGFHMGTVEVNWS
PLFHREARLIPSMSYCRHANGREMEDAAAMLADNPEIARTLITHRFPLEDAVEAFRVAADRKAGALRVVVEP

Specific function: Putative L-galactonate oxidoreductase that is required for growth on L-galactonate as the sole carbon source [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=329, Percent_Identity=28.2674772036474, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI226510992, Length=308, Percent_Identity=28.2467532467532, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788073, Length=346, Percent_Identity=26.3005780346821, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788407, Length=325, Percent_Identity=27.3846153846154, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788075, Length=341, Percent_Identity=23.7536656891496, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790718, Length=314, Percent_Identity=24.8407643312102, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790045, Length=348, Percent_Identity=25, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082125, Length=347, Percent_Identity=28.2420749279539, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787863, Length=341, Percent_Identity=24.633431085044, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=330, Percent_Identity=24.8484848484848, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=307, Percent_Identity=30.2931596091205, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319955, Length=307, Percent_Identity=29.9674267100977, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323099, Length=347, Percent_Identity=25.3602305475504, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319258, Length=374, Percent_Identity=23.2620320855615, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=374, Percent_Identity=24.331550802139, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17737897, Length=330, Percent_Identity=27.8787878787879, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137530, Length=330, Percent_Identity=26.3636363636364, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.14

Molecular weight: Translated: 32834; Mature: 32834

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAARPKAGEITVVDLDEPPGVGDEVIRIAAASICSSDLMYMRFGLERVIGHELAGVRAD
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
GTGAVVEALYGCMECDLCLDGRYNLCPTHIHRALGSSADGGMAEQFRVPSQRLVALPPGL
CCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCHHEEECCCCC
DIRDASLVEPASVSWHALRLAGTGPDTRLAVVGAGALGLLAAAGARRQGAPEVDLEARHP
CCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCC
HQVEAGERLGAKVGTTGLYDVVVEAAGTSASLARAVDLVAPGGTVVVVGFHMGTVEVNWS
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHEECCCCEEEEEEEEEEEEEEECC
PLFHREARLIPSMSYCRHANGREMEDAAAMLADNPEIARTLITHRFPLEDAVEAFRVAAD
HHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHC
RKAGALRVVVEP
CCCCEEEEEECC
>Mature Secondary Structure
MKAARPKAGEITVVDLDEPPGVGDEVIRIAAASICSSDLMYMRFGLERVIGHELAGVRAD
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECC
GTGAVVEALYGCMECDLCLDGRYNLCPTHIHRALGSSADGGMAEQFRVPSQRLVALPPGL
CCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCHHEEECCCCC
DIRDASLVEPASVSWHALRLAGTGPDTRLAVVGAGALGLLAAAGARRQGAPEVDLEARHP
CCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCC
HQVEAGERLGAKVGTTGLYDVVVEAAGTSASLARAVDLVAPGGTVVVVGFHMGTVEVNWS
CCCCHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHEECCCCEEEEEEEEEEEEEEECC
PLFHREARLIPSMSYCRHANGREMEDAAAMLADNPEIARTLITHRFPLEDAVEAFRVAAD
HHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHC
RKAGALRVVVEP
CCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-iditol; NAD+

Specific reaction: L-iditol + NAD+ = L-sorbose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]