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The map label for this gene is hutI

Identifier: 157963982

GI number: 157963982

Start: 5063725

End: 5064969

Strand: Direct

Name: hutI

Synonym: Spea_4171

Alternate gene names: 157963982

Gene position: 5063725-5064969 (Clockwise)

Preceding gene: 157963977

Following gene: 157963988

Centisome position: 97.86

GC content: 52.53

Gene sequence:

>1245_bases
ATGTCGTGGGATCAAGTCTGGATAGATATCAACATCGCTACAATGTCGCCTAATATTTCAGAGCCTTACGGCGCAATTAC
CGATGCCGCGCTCGCCGTTCAAGACGGTAAAATTGCCTGGGTAGGTAAACGCAGCGACCTGCCAGAATTTGATGTATTCG
CCACGCCAATCTACAAGGGAAAAGGTGGCTGGATCACCCCAGGCCTTATCGATGCCCACACGCACTTAGTATTTGCGGGT
AATCGTGCCAACGAGTTTGAACTGCGCCTCAATGGGGCCAGTTACGAAGAGATCGCTCGCAGTGGCGGCGGCATTATTTC
CACCGTTAACGCCTGCCGTGAAGCCGACGAAGCCGAACTATTTGAGCTAGGTCGCCAGCGCCTGAACGCATTAGCAAAAG
AAGGCGTCACCACGGTAGAAATCAAGTCAGGTTACGGCCTAGATATCGAAACTGAACTGAAGATTTTGCGGGTTGCCCGC
GAGCTAGGAAAGCACCACCATGTCGATGTGAAAACCACCTTCTTAGGCGCCCACGCCATTCCGCCAGAATATAAAGACTC
TCCCGGCAGCACAGAAAGGAGTGACGCATACGTCGACTTAGTGATCAACGAGATGTTGCCAAAGGTGATTGCAGAGAACC
TAGCCGATGCCGTGGATGTGTTTTGTGAGAATATCGCCTTCAATCTTGAGCAAACCGAGCGAGTACTCAGCGCAGCAAAA
GATGCAGGACTCGACATCAAGCTACACGCCGAACAGTTATCTAATATGGGCGGCTCAGCCATGGCAGCGAGGCTTGGGGC
AAAGTCGGTGGATCATATCGAATACCTAGATGAAGACGGCGTAAAAGCCCTGAGCGAAAGTGGCACCTGCGCGACCATAC
TCCCCGGCGCGTTTTACTTTTTACGTGAAACCCAGCATCCGCCAATCGATTTACTGCGCAAATACAAGGTACCTATGGTG
GTCGCCAGCGATTACAACCCCGGCTCATCACCACTTTGCTCAAGCCTATTGATGCTCAACATGGCCTGTACCTTATTGCG
CTTAACCCCAGAAGAAGCGTTAGCGGGTATGACGCGCAACGCCGCCAAAGCGTTAGGGATTGAGGACCAAGTCGGCGTTA
TCGAAGTCGGCATGACCGCAGACTTCTGCATGTGGAATATCAGCACCCCAGCAGAGCTGGCCTACACCTATGGCGTGGCC
TCATGTGTGGATGTGGTGAAGAACGGCCACCTAGTACACCAATAG

Upstream 100 bases:

>100_bases
TCCTAGCTTGTATATACAAGTAAACACAAGCTAGCTCACAAATTATTTTTAGTGTTAGTATTCCCTCATCGCAATAAGCA
ACCAAAAAGAGAACCGCATC

Downstream 100 bases:

>100_bases
TTGGGAATGCCTGAGCGATGAGGCATGTTCATCGTTTTGGTGTTTAGGGTGCCACCATTTGGCTATGGTGGCTCCAGCCT
TTTTCGGCCTGCTGGCTACG

Product: imidazolonepropionase

Products: NA

Alternate protein names: Imidazolone-5-propionate hydrolase

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MSWDQVWIDINIATMSPNISEPYGAITDAALAVQDGKIAWVGKRSDLPEFDVFATPIYKGKGGWITPGLIDAHTHLVFAG
NRANEFELRLNGASYEEIARSGGGIISTVNACREADEAELFELGRQRLNALAKEGVTTVEIKSGYGLDIETELKILRVAR
ELGKHHHVDVKTTFLGAHAIPPEYKDSPGSTERSDAYVDLVINEMLPKVIAENLADAVDVFCENIAFNLEQTERVLSAAK
DAGLDIKLHAEQLSNMGGSAMAARLGAKSVDHIEYLDEDGVKALSESGTCATILPGAFYFLRETQHPPIDLLRKYKVPMV
VASDYNPGSSPLCSSLLMLNMACTLLRLTPEEALAGMTRNAAKALGIEDQVGVIEVGMTADFCMWNISTPAELAYTYGVA
SCVDVVKNGHLVHQ

Sequences:

>Translated_414_residues
MSWDQVWIDINIATMSPNISEPYGAITDAALAVQDGKIAWVGKRSDLPEFDVFATPIYKGKGGWITPGLIDAHTHLVFAG
NRANEFELRLNGASYEEIARSGGGIISTVNACREADEAELFELGRQRLNALAKEGVTTVEIKSGYGLDIETELKILRVAR
ELGKHHHVDVKTTFLGAHAIPPEYKDSPGSTERSDAYVDLVINEMLPKVIAENLADAVDVFCENIAFNLEQTERVLSAAK
DAGLDIKLHAEQLSNMGGSAMAARLGAKSVDHIEYLDEDGVKALSESGTCATILPGAFYFLRETQHPPIDLLRKYKVPMV
VASDYNPGSSPLCSSLLMLNMACTLLRLTPEEALAGMTRNAAKALGIEDQVGVIEVGMTADFCMWNISTPAELAYTYGVA
SCVDVVKNGHLVHQ
>Mature_413_residues
SWDQVWIDINIATMSPNISEPYGAITDAALAVQDGKIAWVGKRSDLPEFDVFATPIYKGKGGWITPGLIDAHTHLVFAGN
RANEFELRLNGASYEEIARSGGGIISTVNACREADEAELFELGRQRLNALAKEGVTTVEIKSGYGLDIETELKILRVARE
LGKHHHVDVKTTFLGAHAIPPEYKDSPGSTERSDAYVDLVINEMLPKVIAENLADAVDVFCENIAFNLEQTERVLSAAKD
AGLDIKLHAEQLSNMGGSAMAARLGAKSVDHIEYLDEDGVKALSESGTCATILPGAFYFLRETQHPPIDLLRKYKVPMVV
ASDYNPGSSPLCSSLLMLNMACTLLRLTPEEALAGMTRNAAKALGIEDQVGVIEVGMTADFCMWNISTPAELAYTYGVAS
CVDVVKNGHLVHQ

Specific function: Unknown

COG id: COG1228

COG function: function code Q; Imidazolonepropionase and related amidohydrolases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hutI family

Homologues:

Organism=Homo sapiens, GI223972677, Length=343, Percent_Identity=39.9416909620991, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17555004, Length=377, Percent_Identity=32.8912466843501, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTI_SHEPA (A8HA94)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001504016.1
- ProteinModelPortal:   A8HA94
- SMR:   A8HA94
- STRING:   A8HA94
- GeneID:   5664555
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_4171
- NMPDR:   fig|398579.3.peg.3622
- eggNOG:   COG1228
- HOGENOM:   HBG686142
- OMA:   MNMACTL
- ProtClustDB:   PRK09356
- BioCyc:   SPEA398579:SPEA_4171-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00372
- InterPro:   IPR006680
- InterPro:   IPR005920
- InterPro:   IPR011059
- TIGRFAMs:   TIGR01224

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.7

Molecular weight: Translated: 44852; Mature: 44721

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: BINDING 82-82 BINDING 95-95 BINDING 145-145 BINDING 178-178 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWDQVWIDINIATMSPNISEPYGAITDAALAVQDGKIAWVGKRSDLPEFDVFATPIYKG
CCCCEEEEEEEEEEECCCCCCCCCCHHHHEEEEECCEEEEEECCCCCCCCCEEECCEEEC
KGGWITPGLIDAHTHLVFAGNRANEFELRLNGASYEEIARSGGGIISTVNACREADEAEL
CCCEECCCEECCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCEEHHHHHHHCCCHHHH
FELGRQRLNALAKEGVTTVEIKSGYGLDIETELKILRVARELGKHHHVDVKTTFLGAHAI
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
PPEYKDSPGSTERSDAYVDLVINEMLPKVIAENLADAVDVFCENIAFNLEQTERVLSAAK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DAGLDIKLHAEQLSNMGGSAMAARLGAKSVDHIEYLDEDGVKALSESGTCATILPGAFYF
HCCCEEEEEHHHHCCCCCHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCEEEHHHHHHHH
LRETQHPPIDLLRKYKVPMVVASDYNPGSSPLCSSLLMLNMACTLLRLTPEEALAGMTRN
HHCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHH
AAKALGIEDQVGVIEVGMTADFCMWNISTPAELAYTYGVASCVDVVKNGHLVHQ
HHHHCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SWDQVWIDINIATMSPNISEPYGAITDAALAVQDGKIAWVGKRSDLPEFDVFATPIYKG
CCCEEEEEEEEEEECCCCCCCCCCHHHHEEEEECCEEEEEECCCCCCCCCEEECCEEEC
KGGWITPGLIDAHTHLVFAGNRANEFELRLNGASYEEIARSGGGIISTVNACREADEAEL
CCCEECCCEECCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCEEHHHHHHHCCCHHHH
FELGRQRLNALAKEGVTTVEIKSGYGLDIETELKILRVARELGKHHHVDVKTTFLGAHAI
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCC
PPEYKDSPGSTERSDAYVDLVINEMLPKVIAENLADAVDVFCENIAFNLEQTERVLSAAK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DAGLDIKLHAEQLSNMGGSAMAARLGAKSVDHIEYLDEDGVKALSESGTCATILPGAFYF
HCCCEEEEEHHHHCCCCCHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCEEEHHHHHHHH
LRETQHPPIDLLRKYKVPMVVASDYNPGSSPLCSSLLMLNMACTLLRLTPEEALAGMTRN
HHCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHHH
AAKALGIEDQVGVIEVGMTADFCMWNISTPAELAYTYGVASCVDVVKNGHLVHQ
HHHHCCCCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA