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The map label for this gene is rlmF

Identifier: 157963957

GI number: 157963957

Start: 5035780

End: 5036922

Strand: Reverse

Name: rlmF

Synonym: Spea_4146

Alternate gene names: 157963957

Gene position: 5036922-5035780 (Counterclockwise)

Preceding gene: 157963958

Following gene: 157963956

Centisome position: 97.34

GC content: 47.42

Gene sequence:

>1143_bases
ATGAGTCATAAAACTAAATCTACCACTCAAGAGCGCAAGGCTGGCAAGTCTTCGGCACCTCAACGACAAGTCGTTTCAAA
ATCGAGTTTACAGAAAAAGGTGAGCAAGCCTACTGCGACAGCCAAGCTCCAAAAGAGTAAGGCGCTGCACCCACGAAATG
CCCACAACAATGGCTATGATTTTGCCGCGTTGGTGACGGCATTTCCGAAACTTAAGGCTTTTGTGAAGCCTAACCCCTAT
GGCAATTTGTCGATAGACTTCGCCGATCCTGTAGCGGTTAAAATGCTAAATGCGGCGTTGCTAAAGTTTCACTATGGCGT
TGGCCATTGGGATATTCCCGATGGCTTTCTATGTCCGCCTATCCCTGGACGGGCCGACTATATCCATCATGTGGCCGATC
TGCTGGCGGTTAAAAAGTCGAGTAAGAAGCGCATTCCTAAAGGCCCGAAAGTTCGAGTATTAGATATCGGTACCGGAGCG
AATGTTATCTATCCGCTACTAGGTATTCAATCCTATGGTTGGGACTTTGTTGGCAGCGATGTCGACCCGCTCTCGATTGC
TAATGCACAGCAGATTTTTGCCGGCAATTCCGATATTGCTAATAAGTTTAGCTCTAGGCTGCAAGCTGACTCTAAACACG
TATTTCATGGGGTAATTGAGGCAAATGAGCGCTTCGACCTGACCCTATGCAACCCACCATTTCATGCTTCCTTAGCCGAG
GCAAGTGAGGGCACTGCGCGAAAACTTAAAAATCTTGCTGCCAATCGAGCCAAATCATCTCAGGTTATGTCACCTCAAGT
TCAGCCCTCTGGGAAAGTGAAGCCTGCCACAGATAAAAACCATGGCGTGCTTAACTTTGGCGGCCAGAAAGCCGAGTTGT
GGTGTGAGGGAGGCGAGTTGCAATTTTTACGGACTATGATCGAAGAGAGTCATGACTTTGCAAGCCAGTGTTTATGGTTC
ACTACCTTAGTGTCTAAAAAGGAAAACCTTAACCCGGCAAAGGCCTTATTGGCCAAGATAAAAGCTGAAGAGGTGAAAGA
GATAGAGATGCACCAGGGCAATAAAATTACTCGAGTGTTGGCTTGGACCTTTCTTAAACCTGAGCAGCGAGAGCTTTGGG
TGCAGTATCGAGATGCCCAATAA

Upstream 100 bases:

>100_bases
TTTGGTGGTGAACTTACTTTTCACCAAATATCTATACGATTAATTTTCAATGCAAACGTCAGTAATGTTTGCTCTTTTAC
AGGTGCTAGCGCGTTAAAAC

Downstream 100 bases:

>100_bases
TTGAGTTTTAGACGCTTGTCGTTAGAATGGAAGCGAAATAACTACTCATGTGATTTCAAAAGTTTAAAAACTTATCCTAG
ACTCAAAAGATGTAATTATA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSHKTKSTTQERKAGKSSAPQRQVVSKSSLQKKVSKPTATAKLQKSKALHPRNAHNNGYDFAALVTAFPKLKAFVKPNPY
GNLSIDFADPVAVKMLNAALLKFHYGVGHWDIPDGFLCPPIPGRADYIHHVADLLAVKKSSKKRIPKGPKVRVLDIGTGA
NVIYPLLGIQSYGWDFVGSDVDPLSIANAQQIFAGNSDIANKFSSRLQADSKHVFHGVIEANERFDLTLCNPPFHASLAE
ASEGTARKLKNLAANRAKSSQVMSPQVQPSGKVKPATDKNHGVLNFGGQKAELWCEGGELQFLRTMIEESHDFASQCLWF
TTLVSKKENLNPAKALLAKIKAEEVKEIEMHQGNKITRVLAWTFLKPEQRELWVQYRDAQ

Sequences:

>Translated_380_residues
MSHKTKSTTQERKAGKSSAPQRQVVSKSSLQKKVSKPTATAKLQKSKALHPRNAHNNGYDFAALVTAFPKLKAFVKPNPY
GNLSIDFADPVAVKMLNAALLKFHYGVGHWDIPDGFLCPPIPGRADYIHHVADLLAVKKSSKKRIPKGPKVRVLDIGTGA
NVIYPLLGIQSYGWDFVGSDVDPLSIANAQQIFAGNSDIANKFSSRLQADSKHVFHGVIEANERFDLTLCNPPFHASLAE
ASEGTARKLKNLAANRAKSSQVMSPQVQPSGKVKPATDKNHGVLNFGGQKAELWCEGGELQFLRTMIEESHDFASQCLWF
TTLVSKKENLNPAKALLAKIKAEEVKEIEMHQGNKITRVLAWTFLKPEQRELWVQYRDAQ
>Mature_379_residues
SHKTKSTTQERKAGKSSAPQRQVVSKSSLQKKVSKPTATAKLQKSKALHPRNAHNNGYDFAALVTAFPKLKAFVKPNPYG
NLSIDFADPVAVKMLNAALLKFHYGVGHWDIPDGFLCPPIPGRADYIHHVADLLAVKKSSKKRIPKGPKVRVLDIGTGAN
VIYPLLGIQSYGWDFVGSDVDPLSIANAQQIFAGNSDIANKFSSRLQADSKHVFHGVIEANERFDLTLCNPPFHASLAEA
SEGTARKLKNLAANRAKSSQVMSPQVQPSGKVKPATDKNHGVLNFGGQKAELWCEGGELQFLRTMIEESHDFASQCLWFT
TLVSKKENLNPAKALLAKIKAEEVKEIEMHQGNKITRVLAWTFLKPEQRELWVQYRDAQ

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=322, Percent_Identity=31.3664596273292, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI87081786, Length=327, Percent_Identity=47.4006116207951, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI32564931, Length=329, Percent_Identity=27.0516717325228, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=329, Percent_Identity=25.531914893617, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281363415, Length=325, Percent_Identity=25.5384615384615, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922302, Length=325, Percent_Identity=25.5384615384615, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_SHEPA (A8HA69)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001503991.1
- ProteinModelPortal:   A8HA69
- SMR:   A8HA69
- STRING:   A8HA69
- GeneID:   5664530
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_4146
- NMPDR:   fig|398579.3.peg.3600
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- ProtClustDB:   PRK11727
- BioCyc:   SPEA398579:SPEA_4146-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 41954; Mature: 41822

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKTKSTTQERKAGKSSAPQRQVVSKSSLQKKVSKPTATAKLQKSKALHPRNAHNNGYD
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCH
FAALVTAFPKLKAFVKPNPYGNLSIDFADPVAVKMLNAALLKFHYGVGHWDIPDGFLCPP
HHHHHHHHHHHHHEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
IPGRADYIHHVADLLAVKKSSKKRIPKGPKVRVLDIGTGANVIYPLLGIQSYGWDFVGSD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
VDPLSIANAQQIFAGNSDIANKFSSRLQADSKHVFHGVIEANERFDLTLCNPPFHASLAE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHEECCCCEEEEECCCCCCCHHHH
ASEGTARKLKNLAANRAKSSQVMSPQVQPSGKVKPATDKNHGVLNFGGQKAELWCEGGEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCHH
QFLRTMIEESHDFASQCLWFTTLVSKKENLNPAKALLAKIKAEEVKEIEMHQGNKITRVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AWTFLKPEQRELWVQYRDAQ
HHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure 
SHKTKSTTQERKAGKSSAPQRQVVSKSSLQKKVSKPTATAKLQKSKALHPRNAHNNGYD
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCH
FAALVTAFPKLKAFVKPNPYGNLSIDFADPVAVKMLNAALLKFHYGVGHWDIPDGFLCPP
HHHHHHHHHHHHHEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
IPGRADYIHHVADLLAVKKSSKKRIPKGPKVRVLDIGTGANVIYPLLGIQSYGWDFVGSD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
VDPLSIANAQQIFAGNSDIANKFSSRLQADSKHVFHGVIEANERFDLTLCNPPFHASLAE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHEECCCCEEEEECCCCCCCHHHH
ASEGTARKLKNLAANRAKSSQVMSPQVQPSGKVKPATDKNHGVLNFGGQKAELWCEGGEL
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCHH
QFLRTMIEESHDFASQCLWFTTLVSKKENLNPAKALLAKIKAEEVKEIEMHQGNKITRVL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AWTFLKPEQRELWVQYRDAQ
HHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA