Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 157963935

GI number: 157963935

Start: 5007771

End: 5009228

Strand: Reverse

Name: pleD [H]

Synonym: Spea_4124

Alternate gene names: 157963935

Gene position: 5009228-5007771 (Counterclockwise)

Preceding gene: 157963936

Following gene: 157963934

Centisome position: 96.8

GC content: 44.51

Gene sequence:

>1458_bases
ATGGGCAATAGAGTTTGGAATGATAAAAAGCGCTTTATATGGATTCTATCTCTATTGCTGCTAGTGGCTTTTGCGGTGAC
TAGCGGGATCAGTTATCAAGTTGCCCATGACTCATTAAGTACACAGATTAAAGAGAATACTCTGCCGCTCACTAGCGATA
ATATTTACTCCGAAATTCAGCAAGACCTGCTTAGACCGATTTTTATCTCCTCGTTAATGGCTCAGGATACGTTTGTCAGA
GACTGGACCATTAACCGTGAGCAGACGCCAGATAAACTTATCCGTTATCTCAAAGAGATCCAGGATAAGTACGCTACAGT
CACCAGCTTTTTTGTCTCCGAAGCTTCACGCAAATATTACCACTCTAGCGGTGTATTAAAGCAGATAGAAGATATCGAAC
CGGAGGATGCCTGGTACTTTCGAGTGCGTTCTTTACCTAAGCATGAGCATTATGAAGTGAATATCGACCCCGATACGGCG
GATAAAAACCGGACAGTGATCTATGTTAACTACAAGGTATTTGATTTTGATGGTAATTTCATAGGCGTGACCGGGGTTGG
TCTAGCGGTTGAAAGCGTTAAACGCCTGATTGAGTTATATCAGGCTCGCTATAACCGCAGCGTCTATTTTACCGATAGGC
AAGGTAACGTCACCTTGCACAGTGAACAATACAATGGTGCGGCTAGCCTGCAAACGAGCTCTGGCTTGGAATCTCTCGCC
ACTCGAATTCTCACCAGTCCTAGTGCCTCATTTTCGTATCAGAAAGCGGGAGAGACGGTCTATTTAAACAGCCGCCTAGT
ATCGGAGTTCAAGTGGCATCTGATTGTTGAGCAACAGGAAGATCGAGGCGAGCAAAAGCTATTGACGACCTTCTGGGTCA
ACATAGGTTTAAGCCTATTAATTACTATCGCTATTTTACTTATCGCCAATATGACCTTAGGTAAGTATCAGCGAAAATTA
GAGAGGATGGCCTCGACCGATAAGCTTACAGGTGCGGCTAATCGCCAAGCATTCGAAGACTACTTCGCTAAATCCTTAGC
TAATTCGATATTAGAAAGCTCGCCAATGTCAGTGCTGCTGCTCGATATTGACCATTTTAAGAGGGTCAATGATTGCCACG
GCCATAATATTGGTGACTTAGTGATCCAAACCTTAAGCCATCTCCTGAAAGGCCAGCTAAGGAATGATGATCTTCTGTGT
CGTTGGGGCGGCGAGGAGTTTTTGATCTTGCTACCCGGTTGTAGCTTAAGCCAAGGTAGTGATATTGCCGAAAAGATTAG
AAAGTTAGTGGAGGCCCGACTGGTCACCATAAATGGTTGTGAAATCAATATCACCATAAGCTGTGGCGTGGCAGAGTGCA
GAACATCTGAAAAGGCTGAAGAGCTGATTAATCGTGTCGATATCGCACTCTACCAAGCAAAAGAGCAGGGACGTAATCGA
GTGGTCATGTCTTATTAG

Upstream 100 bases:

>100_bases
TCGATGCGAGTCCCATGCTTTTCAGTTACCATTCACAAAAGTTTAACAACTGCTGAGCTTAGGCTTGCAGCAGTTCACTG
ATGTTGAGTGAGGATGGAGT

Downstream 100 bases:

>100_bases
CGGCTTGATGATGAGATCTGACATCATTTCTATTGGAGTTGGATCAAGTTGCTGCATCTATCGGTGAGGGTGTTAGGATA
GTTTAAGTTTTTCAACAGAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MGNRVWNDKKRFIWILSLLLLVAFAVTSGISYQVAHDSLSTQIKENTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTINREQTPDKLIRYLKEIQDKYATVTSFFVSEASRKYYHSSGVLKQIEDIEPEDAWYFRVRSLPKHEHYEVNIDPDTA
DKNRTVIYVNYKVFDFDGNFIGVTGVGLAVESVKRLIELYQARYNRSVYFTDRQGNVTLHSEQYNGAASLQTSSGLESLA
TRILTSPSASFSYQKAGETVYLNSRLVSEFKWHLIVEQQEDRGEQKLLTTFWVNIGLSLLITIAILLIANMTLGKYQRKL
ERMASTDKLTGAANRQAFEDYFAKSLANSILESSPMSVLLLDIDHFKRVNDCHGHNIGDLVIQTLSHLLKGQLRNDDLLC
RWGGEEFLILLPGCSLSQGSDIAEKIRKLVEARLVTINGCEINITISCGVAECRTSEKAEELINRVDIALYQAKEQGRNR
VVMSY

Sequences:

>Translated_485_residues
MGNRVWNDKKRFIWILSLLLLVAFAVTSGISYQVAHDSLSTQIKENTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVR
DWTINREQTPDKLIRYLKEIQDKYATVTSFFVSEASRKYYHSSGVLKQIEDIEPEDAWYFRVRSLPKHEHYEVNIDPDTA
DKNRTVIYVNYKVFDFDGNFIGVTGVGLAVESVKRLIELYQARYNRSVYFTDRQGNVTLHSEQYNGAASLQTSSGLESLA
TRILTSPSASFSYQKAGETVYLNSRLVSEFKWHLIVEQQEDRGEQKLLTTFWVNIGLSLLITIAILLIANMTLGKYQRKL
ERMASTDKLTGAANRQAFEDYFAKSLANSILESSPMSVLLLDIDHFKRVNDCHGHNIGDLVIQTLSHLLKGQLRNDDLLC
RWGGEEFLILLPGCSLSQGSDIAEKIRKLVEARLVTINGCEINITISCGVAECRTSEKAEELINRVDIALYQAKEQGRNR
VVMSY
>Mature_484_residues
GNRVWNDKKRFIWILSLLLLVAFAVTSGISYQVAHDSLSTQIKENTLPLTSDNIYSEIQQDLLRPIFISSLMAQDTFVRD
WTINREQTPDKLIRYLKEIQDKYATVTSFFVSEASRKYYHSSGVLKQIEDIEPEDAWYFRVRSLPKHEHYEVNIDPDTAD
KNRTVIYVNYKVFDFDGNFIGVTGVGLAVESVKRLIELYQARYNRSVYFTDRQGNVTLHSEQYNGAASLQTSSGLESLAT
RILTSPSASFSYQKAGETVYLNSRLVSEFKWHLIVEQQEDRGEQKLLTTFWVNIGLSLLITIAILLIANMTLGKYQRKLE
RMASTDKLTGAANRQAFEDYFAKSLANSILESSPMSVLLLDIDHFKRVNDCHGHNIGDLVIQTLSHLLKGQLRNDDLLCR
WGGEEFLILLPGCSLSQGSDIAEKIRKLVEARLVTINGCEINITISCGVAECRTSEKAEELINRVDIALYQAKEQGRNRV
VMSY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=40, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=38.4615384615385, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=204, Percent_Identity=37.7450980392157, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787262, Length=200, Percent_Identity=34.5, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=35.625, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=34.3023255813954, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=33.5164835164835, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=33.7278106508876, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787802, Length=139, Percent_Identity=35.2517985611511, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=30.952380952381, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=194, Percent_Identity=26.2886597938144, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=33.5329341317365, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=33.1395348837209, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55217; Mature: 55085

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRVWNDKKRFIWILSLLLLVAFAVTSGISYQVAHDSLSTQIKENTLPLTSDNIYSEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTINREQTPDKLIRYLKEIQDKYATVTSFFVSEASRKYY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSSGVLKQIEDIEPEDAWYFRVRSLPKHEHYEVNIDPDTADKNRTVIYVNYKVFDFDGNF
HHCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVESVKRLIELYQARYNRSVYFTDRQGNVTLHSEQYNGAASLQTSSGLESLA
EEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHH
TRILTSPSASFSYQKAGETVYLNSRLVSEFKWHLIVEQQEDRGEQKLLTTFWVNIGLSLL
HHHHCCCCCCCCHHHCCCEEEECHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ITIAILLIANMTLGKYQRKLERMASTDKLTGAANRQAFEDYFAKSLANSILESSPMSVLL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LDIDHFKRVNDCHGHNIGDLVIQTLSHLLKGQLRNDDLLCRWGGEEFLILLPGCSLSQGS
EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCC
DIAEKIRKLVEARLVTINGCEINITISCGVAECRTSEKAEELINRVDIALYQAKEQGRNR
HHHHHHHHHHHHHEEEEECEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
VVMSY
EEEEC
>Mature Secondary Structure 
GNRVWNDKKRFIWILSLLLLVAFAVTSGISYQVAHDSLSTQIKENTLPLTSDNIYSEIQ
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHH
QDLLRPIFISSLMAQDTFVRDWTINREQTPDKLIRYLKEIQDKYATVTSFFVSEASRKYY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSSGVLKQIEDIEPEDAWYFRVRSLPKHEHYEVNIDPDTADKNRTVIYVNYKVFDFDGNF
HHCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCCEEEEEEEEEEEECCCE
IGVTGVGLAVESVKRLIELYQARYNRSVYFTDRQGNVTLHSEQYNGAASLQTSSGLESLA
EEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHH
TRILTSPSASFSYQKAGETVYLNSRLVSEFKWHLIVEQQEDRGEQKLLTTFWVNIGLSLL
HHHHCCCCCCCCHHHCCCEEEECHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ITIAILLIANMTLGKYQRKLERMASTDKLTGAANRQAFEDYFAKSLANSILESSPMSVLL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
LDIDHFKRVNDCHGHNIGDLVIQTLSHLLKGQLRNDDLLCRWGGEEFLILLPGCSLSQGS
EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCC
DIAEKIRKLVEARLVTINGCEINITISCGVAECRTSEKAEELINRVDIALYQAKEQGRNR
HHHHHHHHHHHHHEEEEECEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
VVMSY
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA