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The map label for this gene is moaE [H]

Identifier: 157963927

GI number: 157963927

Start: 5001551

End: 5001997

Strand: Reverse

Name: moaE [H]

Synonym: Spea_4116

Alternate gene names: 157963927

Gene position: 5001997-5001551 (Counterclockwise)

Preceding gene: 157963928

Following gene: 157963926

Centisome position: 96.66

GC content: 50.34

Gene sequence:

>447_bases
ATGATCCGTGTACAGACTCAGGACTTTAATGTTCCAGAGGAATATCACTTAATCAGCCAAGATAACAGCGATGGCGCTGT
GGTGACCTTTGTCGGTAAAGTGCGTGACTTTAACGATGGCAGCGCGGTAACCGACTTAACGCTTGAGCATTACCCTGGCA
TGACAGAAGCAGTGCTGAATCAGATTGAAGCCGAGGCGCGTGAGCGTTGGCCGCTGAACAATGTCACCATTATTCACCGA
GTCGGCGCTATGGCGCTGGGCGAGCAGATTGTATTTATCGGCGTGACTAGCGCGCACCGTAAAGCGGCATTTGCTGCCTG
TGAGTTCTTGATCGACTTTCTAAAAACCAAAGCCCCGTTTTGGAAACTAGAAGCTGGTGACAAAGGCTCTAACTGGGTTG
AAGCCCGTGACGCCGACGAACAAGCCGCAAAAATGTGGGATAAATAA

Upstream 100 bases:

>100_bases
AAGCTGTTAGTTGCAGTAAACCAAACCATTAGCCAGTGGGATACACCTATTGTAGATGGTGATGAAGTCGCATTCTTCCC
ACCAGTCACTGGAGGTTAAG

Downstream 100 bases:

>100_bases
AGCGTAAAATTAGCGCTGTTAAGGACGCAAGGAGATTGGCTAAATGAAGTTAAGGGTGATGTTACTGGCATTATTGCTGC
CCTTTTTAGCAATGAATAAA

Product: molybdopterin biosynthesis MoaE protein

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEAEARERWPLNNVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGSNWVEARDADEQAAKMWDK

Sequences:

>Translated_148_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEAEARERWPLNNVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGSNWVEARDADEQAAKMWDK
>Mature_148_residues
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLNQIEAEARERWPLNNVTIIHR
VGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPFWKLEAGDKGSNWVEARDADEQAAKMWDK

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=108, Percent_Identity=37.037037037037, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1787003, Length=145, Percent_Identity=59.3103448275862, Blast_Score=182, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17509279, Length=108, Percent_Identity=38.8888888888889, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356259, Length=126, Percent_Identity=36.5079365079365, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 16583; Mature: 16583

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLN
CEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHHHHHHH
QIEAEARERWPLNNVTIIHRVGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPF
HHHHHHHHCCCCCCEEHEEHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
WKLEAGDKGSNWVEARDADEQAAKMWDK
EEEECCCCCCCEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MIRVQTQDFNVPEEYHLISQDNSDGAVVTFVGKVRDFNDGSAVTDLTLEHYPGMTEAVLN
CEEEECCCCCCCHHEEEEECCCCCCEEEEEEECCEECCCCCEEEEEEHHHCCCHHHHHHH
QIEAEARERWPLNNVTIIHRVGAMALGEQIVFIGVTSAHRKAAFAACEFLIDFLKTKAPF
HHHHHHHHCCCCCCEEHEEHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCE
WKLEAGDKGSNWVEARDADEQAAKMWDK
EEEECCCCCCCEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]