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The map label for this gene is acrA [H]

Identifier: 157963872

GI number: 157963872

Start: 4937252

End: 4938403

Strand: Reverse

Name: acrA [H]

Synonym: Spea_4061

Alternate gene names: 157963872

Gene position: 4938403-4937252 (Counterclockwise)

Preceding gene: 157963873

Following gene: 157963871

Centisome position: 95.44

GC content: 45.83

Gene sequence:

>1152_bases
ATGCGGCAAATAGTGAAAATTGCCTCTGTTTTAAGTGTGGCGTTATGGATAACTGGCTGTGAGCCAAAACCTGAGCAGGG
GCCACAACAACGTGGACCTATGGAAGTTGGCGCTATAACAGTTACAGCCCAACCTCAATCGATTATGGTTGAACTACCAG
GTCGTAGTAAGGCATATCTAGAAGCTGAAGTACGTCCACAGGTGAGCGGTATCATTATGACCCGCGGCTTTACTGAAGGT
AGAGATGTTGAGCAAGGTCAGTCTTTATACCAGATCGAACCTTCTACTTACGAAGCTGCACTAGTTAGTGCGAAAGCTGA
TCTTACCAGCGCTGAAGCGGCATTGGTTTCGGCTAAAGCTAAAGCGTTACGCTTTGCGAAACTAATCAAGCTGAACTCTA
TTAGTCAACAAGACTTCGATGAAGCTGATGCGGCTTATAAAGAGGCTATGGCACGTGTAACCGTAGCAAAAGCTGCGATT
AACACAGCGTCGATCAACCTTGAATATACTAAGGTAACAGCACCAATTTCTGGCCGTATCGGTAAGTCTGATGTGACACC
GGGTGCACTTGTTACAGCTAATCAAAGCCAAACATTAGCGACAATTCAGCAGTTAGATCCAATCAATATCGATATCGCCC
AGTCGAGCGCGCAAATGTTGCGTCTTAAGTCTCAACTTAAGCGTGGTCAAATTGAAGCCGCTGCAAATGCGAATGTAGAG
CTAATCCTTGAAGATGGCACTCCATATAGTCATACAGGCGTGCTGCAGTTTGCTGAAGTTACCGTTAATGAAAACACGGG
TTCTGTGATTATTCGTGCCGAATTCCCTAATCCGGAAGGCGTGTTAATGCCGGGCATGTATGTGCGTGCTGTGCTTAATA
CTGGTACCGATCCGGCTGCGATTCTAGTACCACAAAAAGCCATTACTCGTAACACCAAAGGTGAAGCGGTAGCTATGGTT
ATTGATGATGGCAAAGTGGCTGCACGTATTGTGACAACTGCTGAAGTGATTGATAACCAATGGCGTATTCTTGATGGTTT
GAAAGTGGGCGATCAACTAATCGTTGAAGGTCTACAAAAAATTCGTCCAGGCGCCCCAGTAAAGGCGGGTCCTTTGGTCT
CTGACAAGAAACCAGAAACACAACAGAAATAG

Upstream 100 bases:

>100_bases
AAATATTCGACAAAAATTTATGCTTCCAAAGGTGGGATTGACGGCATTATCTGCTTTCAAAAAATGATTACCGAGTAGAT
GGCTTTTATTAGGAGTTCTC

Downstream 100 bases:

>100_bases
GGCAGAGTTATGGCACGTTTCTTTATCGATCGCCCTATTTTTGCATGGGTGATCGCTATTATTGTGATGCTGGCAGGTGT
GCTCTCAATTGTGGGCTTAC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRQIVKIASVLSVALWITGCEPKPEQGPQQRGPMEVGAITVTAQPQSIMVELPGRSKAYLEAEVRPQVSGIIMTRGFTEG
RDVEQGQSLYQIEPSTYEAALVSAKADLTSAEAALVSAKAKALRFAKLIKLNSISQQDFDEADAAYKEAMARVTVAKAAI
NTASINLEYTKVTAPISGRIGKSDVTPGALVTANQSQTLATIQQLDPINIDIAQSSAQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYSHTGVLQFAEVTVNENTGSVIIRAEFPNPEGVLMPGMYVRAVLNTGTDPAAILVPQKAITRNTKGEAVAMV
IDDGKVAARIVTTAEVIDNQWRILDGLKVGDQLIVEGLQKIRPGAPVKAGPLVSDKKPETQQK

Sequences:

>Translated_383_residues
MRQIVKIASVLSVALWITGCEPKPEQGPQQRGPMEVGAITVTAQPQSIMVELPGRSKAYLEAEVRPQVSGIIMTRGFTEG
RDVEQGQSLYQIEPSTYEAALVSAKADLTSAEAALVSAKAKALRFAKLIKLNSISQQDFDEADAAYKEAMARVTVAKAAI
NTASINLEYTKVTAPISGRIGKSDVTPGALVTANQSQTLATIQQLDPINIDIAQSSAQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYSHTGVLQFAEVTVNENTGSVIIRAEFPNPEGVLMPGMYVRAVLNTGTDPAAILVPQKAITRNTKGEAVAMV
IDDGKVAARIVTTAEVIDNQWRILDGLKVGDQLIVEGLQKIRPGAPVKAGPLVSDKKPETQQK
>Mature_383_residues
MRQIVKIASVLSVALWITGCEPKPEQGPQQRGPMEVGAITVTAQPQSIMVELPGRSKAYLEAEVRPQVSGIIMTRGFTEG
RDVEQGQSLYQIEPSTYEAALVSAKADLTSAEAALVSAKAKALRFAKLIKLNSISQQDFDEADAAYKEAMARVTVAKAAI
NTASINLEYTKVTAPISGRIGKSDVTPGALVTANQSQTLATIQQLDPINIDIAQSSAQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYSHTGVLQFAEVTVNENTGSVIIRAEFPNPEGVLMPGMYVRAVLNTGTDPAAILVPQKAITRNTKGEAVAMV
IDDGKVAARIVTTAEVIDNQWRILDGLKVGDQLIVEGLQKIRPGAPVKAGPLVSDKKPETQQK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=50.2717391304348, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1789665, Length=362, Percent_Identity=50.2762430939227, Blast_Score=333, Evalue=9e-93,
Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=46.1325966850829, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI87082036, Length=320, Percent_Identity=25.9375, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081796, Length=335, Percent_Identity=25.6716417910448, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40902; Mature: 40902

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIVKIASVLSVALWITGCEPKPEQGPQQRGPMEVGAITVTAQPQSIMVELPGRSKAYL
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCEEE
EAEVRPQVSGIIMTRGFTEGRDVEQGQSLYQIEPSTYEAALVSAKADLTSAEAALVSAKA
EEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
KALRFAKLIKLNSISQQDFDEADAAYKEAMARVTVAKAAINTASINLEYTKVTAPISGRI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCC
GKSDVTPGALVTANQSQTLATIQQLDPINIDIAQSSAQMLRLKSQLKRGQIEAAANANVE
CCCCCCCCEEEECCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCEEEECCCCEE
LILEDGTPYSHTGVLQFAEVTVNENTGSVIIRAEFPNPEGVLMPGMYVRAVLNTGTDPAA
EEEECCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCCEE
ILVPQKAITRNTKGEAVAMVIDDGKVAARIVTTAEVIDNQWRILDGLKVGDQLIVEGLQK
EEECCHHHHCCCCCCEEEEEEECCCEEEEEEEEHHHHCCCEEEEECCCCCHHHHHHHHHH
IRPGAPVKAGPLVSDKKPETQQK
HCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRQIVKIASVLSVALWITGCEPKPEQGPQQRGPMEVGAITVTAQPQSIMVELPGRSKAYL
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCEEE
EAEVRPQVSGIIMTRGFTEGRDVEQGQSLYQIEPSTYEAALVSAKADLTSAEAALVSAKA
EEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHH
KALRFAKLIKLNSISQQDFDEADAAYKEAMARVTVAKAAINTASINLEYTKVTAPISGRI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEECCCCCCC
GKSDVTPGALVTANQSQTLATIQQLDPINIDIAQSSAQMLRLKSQLKRGQIEAAANANVE
CCCCCCCCEEEECCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCCCEEEECCCCEE
LILEDGTPYSHTGVLQFAEVTVNENTGSVIIRAEFPNPEGVLMPGMYVRAVLNTGTDPAA
EEEECCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCCEE
ILVPQKAITRNTKGEAVAMVIDDGKVAARIVTTAEVIDNQWRILDGLKVGDQLIVEGLQK
EEECCHHHHCCCCCCEEEEEEECCCEEEEEEEEHHHHCCCEEEEECCCCCHHHHHHHHHH
IRPGAPVKAGPLVSDKKPETQQK
HCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]