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The map label for this gene is bioH [H]

Identifier: 157963824

GI number: 157963824

Start: 4874918

End: 4875685

Strand: Direct

Name: bioH [H]

Synonym: Spea_4013

Alternate gene names: 157963824

Gene position: 4874918-4875685 (Clockwise)

Preceding gene: 157963822

Following gene: 157963825

Centisome position: 94.21

GC content: 52.34

Gene sequence:

>768_bases
ATGAGTCAGAGCCAGCTACATATCGAATCTATCGGTCAAGGGCCCGATGTTGTAATGCTTCATGGTTGGGGGGTTAACAG
CGCCGTATTTACTCCACTGCAGGGCGCATTAGCCAAGTATAGGGTTCACTTCGTCGATCTGCCGGGATTTGGCCATAGCC
CAGCGGTCAATGGCGATATCTACGACTGGCTAAACGCCATCTTAGAAGCGGTTCCTGAGCAAGCGATCTGGACTGGCTGG
TCACTCGGCGGACTCATTGCTACATTAGCCGCCATCCATCATCCGCAGCGTGTGTCGGCGCTATGCACCATAGCCTCATC
GCCTTGCTTTATGGCGGGTGAAGACTGGCCGGGGATCCCGGAAAAGGTATTGAGCCAATTCGCCCAACAGCTGACTCAAG
ATCTCGATAAGACCATCGAACGCTTTCTTGCTATCCAAGCCATGGGTAGCGAAACCGCCAAGGGCGATATCAAACAATTA
CGTGAGTTAGTGCTTACTAAACCTCAGGCTCAACAGACAGCTCTGGCCCAAGGATTAGCGATGCTTGAACGGGTCGACTT
ACGTGCCGAGTTATCTCAAATCAACCAACCTTGGCTAAGAGTCTGGGGACGATTAGATGGGTTAGTGCCGCGCAGAATGA
TAAAAGCCATGCCACAGCTGCCGCAAAGCCAAGACCTATTACTTCACAAGGCCTCTCACGCGCCGTTTATTAGCCACCAG
CAAGAGTTTCTCGACGGCTTCAATCTATGGCTCGACGAGCAAGTCTAG

Upstream 100 bases:

>100_bases
GATTGGGTAAAATTGCCGATAACCACCGAGTCCAAGGTGTTTTGCTAATAATGAAATCCTTTTCATTGTTAAGTTTCAAC
GTTAACCCATGAGGCCCAAA

Downstream 100 bases:

>100_bases
TTAAAACAACAAATTTGTTATTCTAAATTTGCTCAATCTAAAGCTAACCAGTCTAAATAGTTAGCTTTATTTTTCGCCTG
CGATTGGTTAATATTTAAAC

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSQSQLHIESIGQGPDVVMLHGWGVNSAVFTPLQGALAKYRVHFVDLPGFGHSPAVNGDIYDWLNAILEAVPEQAIWTGW
SLGGLIATLAAIHHPQRVSALCTIASSPCFMAGEDWPGIPEKVLSQFAQQLTQDLDKTIERFLAIQAMGSETAKGDIKQL
RELVLTKPQAQQTALAQGLAMLERVDLRAELSQINQPWLRVWGRLDGLVPRRMIKAMPQLPQSQDLLLHKASHAPFISHQ
QEFLDGFNLWLDEQV

Sequences:

>Translated_255_residues
MSQSQLHIESIGQGPDVVMLHGWGVNSAVFTPLQGALAKYRVHFVDLPGFGHSPAVNGDIYDWLNAILEAVPEQAIWTGW
SLGGLIATLAAIHHPQRVSALCTIASSPCFMAGEDWPGIPEKVLSQFAQQLTQDLDKTIERFLAIQAMGSETAKGDIKQL
RELVLTKPQAQQTALAQGLAMLERVDLRAELSQINQPWLRVWGRLDGLVPRRMIKAMPQLPQSQDLLLHKASHAPFISHQ
QEFLDGFNLWLDEQV
>Mature_254_residues
SQSQLHIESIGQGPDVVMLHGWGVNSAVFTPLQGALAKYRVHFVDLPGFGHSPAVNGDIYDWLNAILEAVPEQAIWTGWS
LGGLIATLAAIHHPQRVSALCTIASSPCFMAGEDWPGIPEKVLSQFAQQLTQDLDKTIERFLAIQAMGSETAKGDIKQLR
ELVLTKPQAQQTALAQGLAMLERVDLRAELSQINQPWLRVWGRLDGLVPRRMIKAMPQLPQSQDLLLHKASHAPFISHQQ
EFLDGFNLWLDEQV

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=239, Percent_Identity=47.2803347280335, Blast_Score=223, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28249; Mature: 28118

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSQLHIESIGQGPDVVMLHGWGVNSAVFTPLQGALAKYRVHFVDLPGFGHSPAVNGDI
CCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHH
YDWLNAILEAVPEQAIWTGWSLGGLIATLAAIHHPQRVSALCTIASSPCFMAGEDWPGIP
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCEECCCCCCCCH
EKVLSQFAQQLTQDLDKTIERFLAIQAMGSETAKGDIKQLRELVLTKPQAQQTALAQGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
MLERVDLRAELSQINQPWLRVWGRLDGLVPRRMIKAMPQLPQSQDLLLHKASHAPFISHQ
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHH
QEFLDGFNLWLDEQV
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQSQLHIESIGQGPDVVMLHGWGVNSAVFTPLQGALAKYRVHFVDLPGFGHSPAVNGDI
CCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHH
YDWLNAILEAVPEQAIWTGWSLGGLIATLAAIHHPQRVSALCTIASSPCFMAGEDWPGIP
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCEECCCCCCCCH
EKVLSQFAQQLTQDLDKTIERFLAIQAMGSETAKGDIKQLRELVLTKPQAQQTALAQGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
MLERVDLRAELSQINQPWLRVWGRLDGLVPRRMIKAMPQLPQSQDLLLHKASHAPFISHQ
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHH
QEFLDGFNLWLDEQV
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA