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The map label for this gene is dinF [H]

Identifier: 157963820

GI number: 157963820

Start: 4869359

End: 4870690

Strand: Direct

Name: dinF [H]

Synonym: Spea_4009

Alternate gene names: 157963820

Gene position: 4869359-4870690 (Clockwise)

Preceding gene: 157963818

Following gene: 157963822

Centisome position: 94.1

GC content: 47.45

Gene sequence:

>1332_bases
ATGATTGCCTTACTGTTAAACCCACAAAAAAACCGACAACTGTTTGCCCTCGCACTACCGATGATTTTATCAAATATCAC
GGTGCCATTACTGGGACTTGTCGATACCGCCGTTGTTGGACACCTTAGTAACGCCTATTATTTAGGCGGTGTGGCAGTTG
GGTCAACGATTATAACCTTAATTTTGTGGTTGCTAGGATTTTTACGCATGTCGACCACGGGATTAGTCGCTCAAGCCTAT
GGCGCCAACGACACCCAGCAACAATTTAAACTCTTGGTACAAGCAGCAAGTCTAGCACTCCTGTTTGGTATTGCCGCTAT
CGCATTACAGCTACCCATAGTGAATTTAGCCATGGCGTTGTCAGATGCCAGCGTCGAAGTCGAGCGCTATTGCCGAGAGT
ATTTCCAGATCAGGATCTGGTCGACCCCCTTCGCCCTAATGAATTTAGTGCTTCTTGGCTGGCTCCTTGGGCGGCAGCAA
CCTAAGGCCGCGATGTGGCAACTGATTGTGGCAAACCTAGTAAATATCGTATTAGATGTCATCTTCGTGCTAGGTCTTGG
CTGGGGTGTGAAAGGAGCGGCACTCGCCTCTGTGTTCGCCGATATCTCTGGTTTCTTAGTAGCACTCACCATGGTTCGCA
GTCAGCTTGGTAAGCTAGGTGATTTTAAGTTGCTACAACTCATCAAGCAGCTTTCCCTGCAAAGCTACGCCAAGATGATG
AGCCTTAACACAGACATCTTTATTCGTAGCCTATGTTTGCAAGCATCATTTGCCTTTATGACTTTTTACGGCGCAGGCTT
AGGTGATAATACCGTTGCAGCCAATGCCGTATTACTCAACTTATTGCTACTCATCTCCTATGCACTCGATGGCATCGCCT
ATTATGCAGAGGCCGAGGTGGGTCGAGCTCATGGCCAGAAAAATAGCCGTTTAATGCAGGAATCGGTGACATTAGCCTGG
GCATGGTCAGCCATTGCCGCATTAGGCTTTACGCTATTTTTTGCCCTCGCGGGTTCGCAGATTATCGCTATGTTGACCAG
CATCAGTGAAGTACAAGCAGTGGCCGAACAATATCTGATTTGGATTATATTCATCCCCTTGCTCGCCTTTGGCTCCTATC
TTTTTGATGGTGTCTATATCGGTGCAGCTCAGGGCAAGGTGATGCGTAACAGTATGATTCTATCTACTTTTGGCGTATTT
TTCCCGGTCTGGTACCTATTAGAACAGCAGGGTAACTTTGCCTTATGGGCAGCCATGAGCAGCTTTATGCTGGCCCGCAG
TGCGTTACTTTCGCTGCATTACTGGTTTAGGCTCAGACTGATTTTAAACTAG

Upstream 100 bases:

>100_bases
CATGATGATACTGTAGGATCACCGTCGCGTGAGCCGAAAAACCTGTCAGCGCAAGTAGCGCTAGCAACACGTTTTTAACC
ATAAATATTAGACCTCTAAA

Downstream 100 bases:

>100_bases
CTTTTATATTTGCTTTCTTGAGTAAGAAACAAAAAACGCGACCTAGACTTAGAGCTAGAGCCGCGTTTTTTATGCAAATT
CTGCATCCAGTATTACTGGT

Product: MATE efflux family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MIALLLNPQKNRQLFALALPMILSNITVPLLGLVDTAVVGHLSNAYYLGGVAVGSTIITLILWLLGFLRMSTTGLVAQAY
GANDTQQQFKLLVQAASLALLFGIAAIALQLPIVNLAMALSDASVEVERYCREYFQIRIWSTPFALMNLVLLGWLLGRQQ
PKAAMWQLIVANLVNIVLDVIFVLGLGWGVKGAALASVFADISGFLVALTMVRSQLGKLGDFKLLQLIKQLSLQSYAKMM
SLNTDIFIRSLCLQASFAFMTFYGAGLGDNTVAANAVLLNLLLLISYALDGIAYYAEAEVGRAHGQKNSRLMQESVTLAW
AWSAIAALGFTLFFALAGSQIIAMLTSISEVQAVAEQYLIWIIFIPLLAFGSYLFDGVYIGAAQGKVMRNSMILSTFGVF
FPVWYLLEQQGNFALWAAMSSFMLARSALLSLHYWFRLRLILN

Sequences:

>Translated_443_residues
MIALLLNPQKNRQLFALALPMILSNITVPLLGLVDTAVVGHLSNAYYLGGVAVGSTIITLILWLLGFLRMSTTGLVAQAY
GANDTQQQFKLLVQAASLALLFGIAAIALQLPIVNLAMALSDASVEVERYCREYFQIRIWSTPFALMNLVLLGWLLGRQQ
PKAAMWQLIVANLVNIVLDVIFVLGLGWGVKGAALASVFADISGFLVALTMVRSQLGKLGDFKLLQLIKQLSLQSYAKMM
SLNTDIFIRSLCLQASFAFMTFYGAGLGDNTVAANAVLLNLLLLISYALDGIAYYAEAEVGRAHGQKNSRLMQESVTLAW
AWSAIAALGFTLFFALAGSQIIAMLTSISEVQAVAEQYLIWIIFIPLLAFGSYLFDGVYIGAAQGKVMRNSMILSTFGVF
FPVWYLLEQQGNFALWAAMSSFMLARSALLSLHYWFRLRLILN
>Mature_443_residues
MIALLLNPQKNRQLFALALPMILSNITVPLLGLVDTAVVGHLSNAYYLGGVAVGSTIITLILWLLGFLRMSTTGLVAQAY
GANDTQQQFKLLVQAASLALLFGIAAIALQLPIVNLAMALSDASVEVERYCREYFQIRIWSTPFALMNLVLLGWLLGRQQ
PKAAMWQLIVANLVNIVLDVIFVLGLGWGVKGAALASVFADISGFLVALTMVRSQLGKLGDFKLLQLIKQLSLQSYAKMM
SLNTDIFIRSLCLQASFAFMTFYGAGLGDNTVAANAVLLNLLLLISYALDGIAYYAEAEVGRAHGQKNSRLMQESVTLAW
AWSAIAALGFTLFFALAGSQIIAMLTSISEVQAVAEQYLIWIIFIPLLAFGSYLFDGVYIGAAQGKVMRNSMILSTFGVF
FPVWYLLEQQGNFALWAAMSSFMLARSALLSLHYWFRLRLILN

Specific function: Unknown

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790477, Length=418, Percent_Identity=45.6937799043062, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48500; Mature: 48500

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALLLNPQKNRQLFALALPMILSNITVPLLGLVDTAVVGHLSNAYYLGGVAVGSTIITL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHH
ILWLLGFLRMSTTGLVAQAYGANDTQQQFKLLVQAASLALLFGIAAIALQLPIVNLAMAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDASVEVERYCREYFQIRIWSTPFALMNLVLLGWLLGRQQPKAAMWQLIVANLVNIVLDV
CCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IFVLGLGWGVKGAALASVFADISGFLVALTMVRSQLGKLGDFKLLQLIKQLSLQSYAKMM
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SLNTDIFIRSLCLQASFAFMTFYGAGLGDNTVAANAVLLNLLLLISYALDGIAYYAEAEV
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAHGQKNSRLMQESVTLAWAWSAIAALGFTLFFALAGSQIIAMLTSISEVQAVAEQYLI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIIFIPLLAFGSYLFDGVYIGAAQGKVMRNSMILSTFGVFFPVWYLLEQQGNFALWAAMS
HHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SFMLARSALLSLHYWFRLRLILN
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIALLLNPQKNRQLFALALPMILSNITVPLLGLVDTAVVGHLSNAYYLGGVAVGSTIITL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHH
ILWLLGFLRMSTTGLVAQAYGANDTQQQFKLLVQAASLALLFGIAAIALQLPIVNLAMAL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDASVEVERYCREYFQIRIWSTPFALMNLVLLGWLLGRQQPKAAMWQLIVANLVNIVLDV
CCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IFVLGLGWGVKGAALASVFADISGFLVALTMVRSQLGKLGDFKLLQLIKQLSLQSYAKMM
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SLNTDIFIRSLCLQASFAFMTFYGAGLGDNTVAANAVLLNLLLLISYALDGIAYYAEAEV
HCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRAHGQKNSRLMQESVTLAWAWSAIAALGFTLFFALAGSQIIAMLTSISEVQAVAEQYLI
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WIIFIPLLAFGSYLFDGVYIGAAQGKVMRNSMILSTFGVFFPVWYLLEQQGNFALWAAMS
HHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SFMLARSALLSLHYWFRLRLILN
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503 [H]