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The map label for this gene is rsmD [H]

Identifier: 157963792

GI number: 157963792

Start: 4837891

End: 4838520

Strand: Direct

Name: rsmD [H]

Synonym: Spea_3981

Alternate gene names: 157963792

Gene position: 4837891-4838520 (Clockwise)

Preceding gene: 157963787

Following gene: 157963793

Centisome position: 93.49

GC content: 47.46

Gene sequence:

>630_bases
ATGGTCAAAAAAAGACCTTCTAGCGGTCAAGTTAGGATTATTTCAGGCCAGTGGCGCTCTCGAAAGTTGCCCATTCACGA
CCTTGAAGGGCTACGCCCAACCACAGATCGCGTTAGAGAAACGCTGTTCAATTGGATTGCTAACGATGTGCGCGGTTCCC
GCGTACTCGACTGCTTTGCTGGTAGCGGTGCATTAAGCCTTGAGTCACTGTCACGATATGCCAGCTACGCCAAGATTTTC
GAGCTTCAGGCTCAAGCGGCGAAACAGCTTATAGCCAACCTAGCCACATTAAAATGCGATAACGCAGATGTCATCAAGGG
TGACAGCCTAGCGTTACTGGCGAATAAGCCAAGCGAAGGCTTCGATCTAGTCTTTATCGACCCGCCTTTTAGAAAGTCAC
TGGCAGAGAAAAGCATTAGCCTTATCGATCAGCACCAATGGCTAAACCATGATGCGCTGATCTATGTGGAAACAGAGAGT
GAAATATCGCTGCTTAACGTACCTGCTAGCTGGGCATTACTGAAAGAGAAAAAAGCAGGCCAAGTGATCTACCGTTTATA
TCAGTATTCAGCTGAAGCGGTTATTGAAGAGCTTAATGGCACGAGTCGCGATACAGAGGAAACAAACTAA

Upstream 100 bases:

>100_bases
GCTTTTTACCTTTTAGCTGTGCTTTGATATAAAATAACGACCTAAATTCAGACGCCATAGTCTACCACTTTCTTTCATTA
GGCAAAATCACGAGGACACC

Downstream 100 bases:

>100_bases
ATGAAAGTCATGCTAGTTCTAGGAAGAATTGTCACCGCGCTTGCTTGGTTGATGATGCTATATAATTTAATTTCGCCCTT
TGAAGGCGGCATTGCAGTGG

Product: putative methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWIANDVRGSRVLDCFAGSGALSLESLSRYASYAKIF
ELQAQAAKQLIANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNHDALIYVETES
EISLLNVPASWALLKEKKAGQVIYRLYQYSAEAVIEELNGTSRDTEETN

Sequences:

>Translated_209_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWIANDVRGSRVLDCFAGSGALSLESLSRYASYAKIF
ELQAQAAKQLIANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNHDALIYVETES
EISLLNVPASWALLKEKKAGQVIYRLYQYSAEAVIEELNGTSRDTEETN
>Mature_209_residues
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWIANDVRGSRVLDCFAGSGALSLESLSRYASYAKIF
ELQAQAAKQLIANLATLKCDNADVIKGDSLALLANKPSEGFDLVFIDPPFRKSLAEKSISLIDQHQWLNHDALIYVETES
EISLLNVPASWALLKEKKAGQVIYRLYQYSAEAVIEELNGTSRDTEETN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=193, Percent_Identity=55.440414507772, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23408; Mature: 23408

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWIANDVRGSRVLDCFA
CCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSGALSLESLSRYASYAKIFELQAQAAKQLIANLATLKCDNADVIKGDSLALLANKPSEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCEEEEECCCCCC
FDLVFIDPPFRKSLAEKSISLIDQHQWLNHDALIYVETESEISLLNVPASWALLKEKKAG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHH
QVIYRLYQYSAEAVIEELNGTSRDTEETN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVKKRPSSGQVRIISGQWRSRKLPIHDLEGLRPTTDRVRETLFNWIANDVRGSRVLDCFA
CCCCCCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
GSGALSLESLSRYASYAKIFELQAQAAKQLIANLATLKCDNADVIKGDSLALLANKPSEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCEEEEECCCCCC
FDLVFIDPPFRKSLAEKSISLIDQHQWLNHDALIYVETESEISLLNVPASWALLKEKKAG
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHH
QVIYRLYQYSAEAVIEELNGTSRDTEETN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]