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The map label for this gene is menE [H]

Identifier: 157963785

GI number: 157963785

Start: 4828495

End: 4829949

Strand: Direct

Name: menE [H]

Synonym: Spea_3974

Alternate gene names: 157963785

Gene position: 4828495-4829949 (Clockwise)

Preceding gene: 157963784

Following gene: 157963787

Centisome position: 93.31

GC content: 51.2

Gene sequence:

>1455_bases
ATGCAAGCTCAGGCAATATCACCAATACATCAAGTTGCTCAGCGTAATAGCGCTGCACAGGCTTACCGGTTTATGGGGCA
AAGCGTTAGCTACGGCTCACTTAGCCAAGGTGTTATTTCGTTAGCCGAGCAGCTATCAGCCATGGGCTTAAAACGCGGTG
ACCGCTTAGCTTGTATCGGCCATAACTGTCCGCAACTACTGCAGATCTACTGGGCTTGTGTCGATAGCGGTGTACTCTTC
TGCCCCATCTCACCGCGTTTTACCAGCCAGCAAATTATCGAGCTGATGCAGACTCATAGTTATCACCGCTATTGGCTCGC
AGCTGACACCCCGGCGGACTCGACACTGATTCAAGCCTTAAATCATCAAAACCAGTTTGAGTCAGTGACGCTAGATTTTA
CTCGCCAAAGCCAGCGGTCAGCGCCAGCCCTAGAGCCTCACATCGCCGTTAACGCTATTCTCACCTCCGGCAGCAGCGGT
ACACCTAAAGCCGCCGCCCACAGCCTAGCGAATCATATCGCCAGCGCCAAAGGTTCGAGAGAATTAATAAAGTTAGCAGC
AGAAGATAGCTGGTTATTGTCGCTACCACTGTTTCATATCGGTGGCCTCGCGATTTTAAACCGCTGCGCCCTCGCAGGCG
CCTGCGTGGTATTTGAAGACAAGCGGCTATCACTCGCCCAGCAGCTTGTTCGTGACAAGATTAGCCACCTCTCCTTAGTG
AGCGCACAACTGCAGCAGCTGCTTGCAAATAGTGATAGCGAAAATGCCAATAACGACTGCTTAAACCAAGTCAAAGCCAT
GCTACTCGGAGGCGGCGCGATCTCAGGCGACCTATTAGCAGAATTAGCCAAGCGCGACATAAAGGCCTACACCAGCTACG
GCATGACAGAGATGGGATCGCAGATCACCACAGGCATTGCCCGCACCGACGGATCCAGCGGCAAGCTGTTATCGGGACGA
GAACTGAAGATTATCGATGGTGAAATATGGCTAAAAGGCGAGTGTCTGTTTCTTGGATATTTAAGCGAGGACGGTTTAAA
TTTACCGCTCAATAAAGACGGCTGGTTCTACACCAAAGATCTAGGCCACTTTGATGCAAACGCCAATCTCTGCATCGACG
GCCGCGCCGATAACATGTTTATCAGCGGCGGCGAAAACATCCAACCCGAAGAGATAGAAGCCGCTTTAAAGCTGCACCCG
TTAATCAGCGATGCCATCGTGTTCCCTGTGGCAGATGCCAAGTTTGGTAATCTCCCAGCAGCCATTCTTAAGTTAAATGA
GAGTAAGCTGAATAACAGTTCAGCTAACCAGCCTTTGGAAGCCGAGCTAACAGAGTTCCTCGCCGACAAGATCGCCCGCT
TCAAAAGGCCAAGGCAATATTACCCTTGGCCAGAGGTCAGCAGCGCCGGGATTAAGGTTGTACGCAAACAGGTGATAGAG
GCAGTTAAGCGTTAG

Upstream 100 bases:

>100_bases
CACTAATCAGCCTAAGCTAGTCAAAACCAGATTAGAACTAATCCTTGGTCAGTGTTAGTTTGGCTCAATCAAAAAGATAA
ATTCGATAACGGATCTCAGC

Downstream 100 bases:

>100_bases
CGACTTAATCACCAGCGCTGCTTTGCGGCATCCAGCTTTTAAGGCACGAAGTGAGTTGACTTGTTAAGTGCTTTCATTGG
ATTGCTCTTTGATTTGTTTT

Product: O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQAQAISPIHQVAQRNSAAQAYRFMGQSVSYGSLSQGVISLAEQLSAMGLKRGDRLACIGHNCPQLLQIYWACVDSGVLF
CPISPRFTSQQIIELMQTHSYHRYWLAADTPADSTLIQALNHQNQFESVTLDFTRQSQRSAPALEPHIAVNAILTSGSSG
TPKAAAHSLANHIASAKGSRELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVVFEDKRLSLAQQLVRDKISHLSLV
SAQLQQLLANSDSENANNDCLNQVKAMLLGGGAISGDLLAELAKRDIKAYTSYGMTEMGSQITTGIARTDGSSGKLLSGR
ELKIIDGEIWLKGECLFLGYLSEDGLNLPLNKDGWFYTKDLGHFDANANLCIDGRADNMFISGGENIQPEEIEAALKLHP
LISDAIVFPVADAKFGNLPAAILKLNESKLNNSSANQPLEAELTEFLADKIARFKRPRQYYPWPEVSSAGIKVVRKQVIE
AVKR

Sequences:

>Translated_484_residues
MQAQAISPIHQVAQRNSAAQAYRFMGQSVSYGSLSQGVISLAEQLSAMGLKRGDRLACIGHNCPQLLQIYWACVDSGVLF
CPISPRFTSQQIIELMQTHSYHRYWLAADTPADSTLIQALNHQNQFESVTLDFTRQSQRSAPALEPHIAVNAILTSGSSG
TPKAAAHSLANHIASAKGSRELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVVFEDKRLSLAQQLVRDKISHLSLV
SAQLQQLLANSDSENANNDCLNQVKAMLLGGGAISGDLLAELAKRDIKAYTSYGMTEMGSQITTGIARTDGSSGKLLSGR
ELKIIDGEIWLKGECLFLGYLSEDGLNLPLNKDGWFYTKDLGHFDANANLCIDGRADNMFISGGENIQPEEIEAALKLHP
LISDAIVFPVADAKFGNLPAAILKLNESKLNNSSANQPLEAELTEFLADKIARFKRPRQYYPWPEVSSAGIKVVRKQVIE
AVKR
>Mature_484_residues
MQAQAISPIHQVAQRNSAAQAYRFMGQSVSYGSLSQGVISLAEQLSAMGLKRGDRLACIGHNCPQLLQIYWACVDSGVLF
CPISPRFTSQQIIELMQTHSYHRYWLAADTPADSTLIQALNHQNQFESVTLDFTRQSQRSAPALEPHIAVNAILTSGSSG
TPKAAAHSLANHIASAKGSRELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVVFEDKRLSLAQQLVRDKISHLSLV
SAQLQQLLANSDSENANNDCLNQVKAMLLGGGAISGDLLAELAKRDIKAYTSYGMTEMGSQITTGIARTDGSSGKLLSGR
ELKIIDGEIWLKGECLFLGYLSEDGLNLPLNKDGWFYTKDLGHFDANANLCIDGRADNMFISGGENIQPEEIEAALKLHP
LISDAIVFPVADAKFGNLPAAILKLNESKLNNSSANQPLEAELTEFLADKIARFKRPRQYYPWPEVSSAGIKVVRKQVIE
AVKR

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=22.4334600760456, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI115511026, Length=474, Percent_Identity=23.8396624472574, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=469, Percent_Identity=23.6673773987207, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI42544132, Length=469, Percent_Identity=23.6673773987207, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI27477105, Length=134, Percent_Identity=35.8208955223881, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788595, Length=480, Percent_Identity=32.7083333333333, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI145693145, Length=501, Percent_Identity=24.5508982035928, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI221142682, Length=474, Percent_Identity=24.0506329113924, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788107, Length=509, Percent_Identity=20.8251473477407, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786810, Length=511, Percent_Identity=22.3091976516634, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=26.4887063655031, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=470, Percent_Identity=23.4042553191489, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=480, Percent_Identity=27.5, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=339, Percent_Identity=27.7286135693215, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17557194, Length=478, Percent_Identity=25.7322175732218, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=509, Percent_Identity=21.8074656188605, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=364, Percent_Identity=24.4505494505494, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=364, Percent_Identity=24.4505494505494, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=364, Percent_Identity=24.4505494505494, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=286, Percent_Identity=25.5244755244755, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=501, Percent_Identity=20.7584830339321, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=412, Percent_Identity=24.2718446601942, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24581924, Length=528, Percent_Identity=25.5681818181818, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648260, Length=502, Percent_Identity=24.1035856573705, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=477, Percent_Identity=23.0607966457023, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19921316, Length=157, Percent_Identity=32.484076433121, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859661, Length=334, Percent_Identity=23.9520958083832, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI20130357, Length=301, Percent_Identity=27.2425249169435, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52613; Mature: 52613

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQAISPIHQVAQRNSAAQAYRFMGQSVSYGSLSQGVISLAEQLSAMGLKRGDRLACIG
CCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
HNCPQLLQIYWACVDSGVLFCPISPRFTSQQIIELMQTHSYHRYWLAADTPADSTLIQAL
CCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
NHQNQFESVTLDFTRQSQRSAPALEPHIAVNAILTSGSSGTPKAAAHSLANHIASAKGSR
HCCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCH
ELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVVFEDKRLSLAQQLVRDKISHLSLV
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHH
SAQLQQLLANSDSENANNDCLNQVKAMLLGGGAISGDLLAELAKRDIKAYTSYGMTEMGS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCH
QITTGIARTDGSSGKLLSGRELKIIDGEIWLKGECLFLGYLSEDGLNLPLNKDGWFYTKD
HHHHHHHCCCCCCCCEECCCEEEEECCEEEEECCEEEEEEECCCCCCCCCCCCCCEEECC
LGHFDANANLCIDGRADNMFISGGENIQPEEIEAALKLHPLISDAIVFPVADAKFGNLPA
CCCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCH
AILKLNESKLNNSSANQPLEAELTEFLADKIARFKRPRQYYPWPEVSSAGIKVVRKQVIE
HHEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AVKR
HHCC
>Mature Secondary Structure
MQAQAISPIHQVAQRNSAAQAYRFMGQSVSYGSLSQGVISLAEQLSAMGLKRGDRLACIG
CCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEC
HNCPQLLQIYWACVDSGVLFCPISPRFTSQQIIELMQTHSYHRYWLAADTPADSTLIQAL
CCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
NHQNQFESVTLDFTRQSQRSAPALEPHIAVNAILTSGSSGTPKAAAHSLANHIASAKGSR
HCCCCCCEEEEEEHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCH
ELIKLAAEDSWLLSLPLFHIGGLAILNRCALAGACVVFEDKRLSLAQQLVRDKISHLSLV
HHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHHHHHHHHH
SAQLQQLLANSDSENANNDCLNQVKAMLLGGGAISGDLLAELAKRDIKAYTSYGMTEMGS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCH
QITTGIARTDGSSGKLLSGRELKIIDGEIWLKGECLFLGYLSEDGLNLPLNKDGWFYTKD
HHHHHHHCCCCCCCCEECCCEEEEECCEEEEECCEEEEEEECCCCCCCCCCCCCCEEECC
LGHFDANANLCIDGRADNMFISGGENIQPEEIEAALKLHPLISDAIVFPVADAKFGNLPA
CCCCCCCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCH
AILKLNESKLNNSSANQPLEAELTEFLADKIARFKRPRQYYPWPEVSSAGIKVVRKQVIE
HHEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
AVKR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]