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The map label for this gene is trmL [H]

Identifier: 157963757

GI number: 157963757

Start: 4799324

End: 4799785

Strand: Direct

Name: trmL [H]

Synonym: Spea_3946

Alternate gene names: 157963757

Gene position: 4799324-4799785 (Clockwise)

Preceding gene: 157963755

Following gene: 157963760

Centisome position: 92.75

GC content: 48.05

Gene sequence:

>462_bases
ATGTTCCATATCGCGCTTTATGAACCCGAAATAGCCCCAAATACCGGTAATATCATCCGCTTAATCGCTAACAACGGCTG
CAAGTTGCATCTGATTGAACCTCTGGGCTTCGATTTGGAAGATAAAAAGCTACGCCGTGCGGGTTTAGACTATGCCGATT
TGGCCAATGTGACCCATCATAAAAATTTCGAGGCCTTCCTTGAAGCCATGGCAGGAAAGCGCATCATGGCCTGTACCACT
AAGGGCAGTCGACCACACTCAGAGCTAACTTTTAAGCAAGATGATGTGTTGCTATTTGGCCCTGAAAGCCGCGGCCTACC
AGCCGATATCATCGACTCGATTCCAACCGAGCAGCGTTTACGTATTCCAATGATGTCCACTAGTCGCAGCTTAAACCTGT
CGAATGCGGTAGCCATTATCAGCTACGAAGCTTGGCGTCAGCTAGATTACGCAGGAATGTAA

Upstream 100 bases:

>100_bases
AGTAACAATAGCTGTTGCTAAGGCGCGCCAATAAGATGAATTTTCCGTGCTGGAATTTTTGCCGTATAATCTCGGCTCAC
CAATTTTTAGGCAGGCCAGC

Downstream 100 bases:

>100_bases
CTGCGCCGACTTCAAGCATAAATGTTCCAGACACATAAAAGCCGCGAATATCGCGGCTTCTTTATGTCTGCACTTAAGCG
TTTACAGCTCTAATCGAGCT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MFHIALYEPEIAPNTGNIIRLIANNGCKLHLIEPLGFDLEDKKLRRAGLDYADLANVTHHKNFEAFLEAMAGKRIMACTT
KGSRPHSELTFKQDDVLLFGPESRGLPADIIDSIPTEQRLRIPMMSTSRSLNLSNAVAIISYEAWRQLDYAGM

Sequences:

>Translated_153_residues
MFHIALYEPEIAPNTGNIIRLIANNGCKLHLIEPLGFDLEDKKLRRAGLDYADLANVTHHKNFEAFLEAMAGKRIMACTT
KGSRPHSELTFKQDDVLLFGPESRGLPADIIDSIPTEQRLRIPMMSTSRSLNLSNAVAIISYEAWRQLDYAGM
>Mature_153_residues
MFHIALYEPEIAPNTGNIIRLIANNGCKLHLIEPLGFDLEDKKLRRAGLDYADLANVTHHKNFEAFLEAMAGKRIMACTT
KGSRPHSELTFKQDDVLLFGPESRGLPADIIDSIPTEQRLRIPMMSTSRSLNLSNAVAIISYEAWRQLDYAGM

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=59.8684210526316, Blast_Score=203, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17156; Mature: 17156

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHIALYEPEIAPNTGNIIRLIANNGCKLHLIEPLGFDLEDKKLRRAGLDYADLANVTHH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHHCCCCCC
KNFEAFLEAMAGKRIMACTTKGSRPHSELTFKQDDVLLFGPESRGLPADIIDSIPTEQRL
CCHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMMSTSRSLNLSNAVAIISYEAWRQLDYAGM
ECCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCC
>Mature Secondary Structure
MFHIALYEPEIAPNTGNIIRLIANNGCKLHLIEPLGFDLEDKKLRRAGLDYADLANVTHH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCHHHHCCCCCC
KNFEAFLEAMAGKRIMACTTKGSRPHSELTFKQDDVLLFGPESRGLPADIIDSIPTEQRL
CCHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMMSTSRSLNLSNAVAIISYEAWRQLDYAGM
ECCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA