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The map label for this gene is gmk [H]

Identifier: 157963688

GI number: 157963688

Start: 4723151

End: 4723783

Strand: Direct

Name: gmk [H]

Synonym: Spea_3877

Alternate gene names: 157963688

Gene position: 4723151-4723783 (Clockwise)

Preceding gene: 157963684

Following gene: 157963689

Centisome position: 91.28

GC content: 48.03

Gene sequence:

>633_bases
ATGCAGCTAATGACCGCTCGCGGAAACCTATTTATCGTCTCGGCGCCAAGTGGCGCAGGTAAATCATCGCTTATTTCGGC
CCTGTTACGAGACCAGCCTGTAGATATGCAAGTTTCAGTTTCGCACACCACAAGGCAGCCGCGTCCAGGTGAAGAGAATG
GTCAGCACTATCACTTCGTTAATCAAGACGAGTTTAAGAGCTTAATTGCCGATGGCGCTTTTTTTGAGTGGGCAGAAGTA
TTTGGTAACTATTATGGCACCTCTCGTGTCACGATTGAGCAAACACTCGCGCAAGGTATCGACGTCTTTCTCGATATCGA
CTGGCAGGGTGCGCAGCAAGTTAAAGAGCTGATGCCTGAATCGATTGGCGTTTTCATTCTCCCGCCGTCAAAAACTGAGC
TAGAACGCCGCCTAACAGGCCGCGGACAAGACACCGCTGAAGTGATTGCGGGCCGCATGGCACAGGCGGTTTCAGAGATG
TCACACTACAATGAATATGACTTCGTTATTGTTAACGATGATTTCGACAAAGCATTAGCCGATTTATTAGCGATCATTCG
CAGCCAAAGATTAACCTCTGCTAGCCAAATTCATACGCAGAATGGTATGATTAAAGATCTGTTGGCAGGCTAA

Upstream 100 bases:

>100_bases
GCGACTAGCGCAAATATCACCCTAGGCTGTATAATGGCTCGCTTAACCTTTTTACCGTCCAGTTTAGCTAACAACAGCTC
TATTGGCACAAACATCGGAT

Downstream 100 bases:

>100_bases
CTGTCTTCATGTACAATTTTGCGTCATTTTTTCAATCGATAAAACACTGGAGTTTCCAAACATGGCTCGCGTAACTGTAG
AAGATGCCGTAAACCAAATC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQLMTARGNLFIVSAPSGAGKSSLISALLRDQPVDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKSLIADGAFFEWAEV
FGNYYGTSRVTIEQTLAQGIDVFLDIDWQGAQQVKELMPESIGVFILPPSKTELERRLTGRGQDTAEVIAGRMAQAVSEM
SHYNEYDFVIVNDDFDKALADLLAIIRSQRLTSASQIHTQNGMIKDLLAG

Sequences:

>Translated_210_residues
MQLMTARGNLFIVSAPSGAGKSSLISALLRDQPVDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKSLIADGAFFEWAEV
FGNYYGTSRVTIEQTLAQGIDVFLDIDWQGAQQVKELMPESIGVFILPPSKTELERRLTGRGQDTAEVIAGRMAQAVSEM
SHYNEYDFVIVNDDFDKALADLLAIIRSQRLTSASQIHTQNGMIKDLLAG
>Mature_210_residues
MQLMTARGNLFIVSAPSGAGKSSLISALLRDQPVDMQVSVSHTTRQPRPGEENGQHYHFVNQDEFKSLIADGAFFEWAEV
FGNYYGTSRVTIEQTLAQGIDVFLDIDWQGAQQVKELMPESIGVFILPPSKTELERRLTGRGQDTAEVIAGRMAQAVSEM
SHYNEYDFVIVNDDFDKALADLLAIIRSQRLTSASQIHTQNGMIKDLLAG

Specific function: Essential for recycling GMP and indirectly, cGMP [H]

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain [H]

Homologues:

Organism=Homo sapiens, GI226874831, Length=174, Percent_Identity=43.1034482758621, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI226874833, Length=174, Percent_Identity=43.1034482758621, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI4504221, Length=174, Percent_Identity=43.1034482758621, Blast_Score=139, Evalue=1e-33,
Organism=Homo sapiens, GI38570142, Length=182, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI14780902, Length=199, Percent_Identity=30.1507537688442, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI21361598, Length=195, Percent_Identity=30.2564102564103, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI262118259, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4505237, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI262118255, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI262118263, Length=179, Percent_Identity=32.4022346368715, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI186700629, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186972120, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI186700627, Length=178, Percent_Identity=26.9662921348315, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI52630447, Length=188, Percent_Identity=27.6595744680851, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI21389483, Length=183, Percent_Identity=27.8688524590164, Blast_Score=67, Evalue=8e-12,
Organism=Homo sapiens, GI111154074, Length=163, Percent_Identity=30.0613496932515, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790080, Length=205, Percent_Identity=68.780487804878, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI193203132, Length=180, Percent_Identity=36.1111111111111, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17508835, Length=180, Percent_Identity=36.1111111111111, Blast_Score=132, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17557440, Length=184, Percent_Identity=28.2608695652174, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71984092, Length=157, Percent_Identity=30.5732484076433, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988041, Length=187, Percent_Identity=25.1336898395722, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71988048, Length=187, Percent_Identity=25.1336898395722, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=44.2622950819672, Blast_Score=155, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21356141, Length=183, Percent_Identity=40.4371584699454, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI85816106, Length=215, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551002, Length=215, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI85724806, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554481, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45554489, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI85816281, Length=215, Percent_Identity=26.046511627907, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007136, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640542, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI161077626, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665 [H]

Pfam domain/function: PF00625 Guanylate_kin [H]

EC number: =2.7.4.8 [H]

Molecular weight: Translated: 23295; Mature: 23295

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00856 GUANYLATE_KINASE_1 ; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLMTARGNLFIVSAPSGAGKSSLISALLRDQPVDMQVSVSHTTRQPRPGEENGQHYHFV
CCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
NQDEFKSLIADGAFFEWAEVFGNYYGTSRVTIEQTLAQGIDVFLDIDWQGAQQVKELMPE
CHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCEEEEECCCCCHHHHHHHCHH
SIGVFILPPSKTELERRLTGRGQDTAEVIAGRMAQAVSEMSHYNEYDFVIVNDDFDKALA
HCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
DLLAIIRSQRLTSASQIHTQNGMIKDLLAG
HHHHHHHHHHCCHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MQLMTARGNLFIVSAPSGAGKSSLISALLRDQPVDMQVSVSHTTRQPRPGEENGQHYHFV
CCEEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
NQDEFKSLIADGAFFEWAEVFGNYYGTSRVTIEQTLAQGIDVFLDIDWQGAQQVKELMPE
CHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHCCCEEEEECCCCCHHHHHHHCHH
SIGVFILPPSKTELERRLTGRGQDTAEVIAGRMAQAVSEMSHYNEYDFVIVNDDFDKALA
HCCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHH
DLLAIIRSQRLTSASQIHTQNGMIKDLLAG
HHHHHHHHHHCCHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]