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The map label for this gene is dgoD [H]

Identifier: 157963669

GI number: 157963669

Start: 4688627

End: 4689748

Strand: Direct

Name: dgoD [H]

Synonym: Spea_3858

Alternate gene names: 157963669

Gene position: 4688627-4689748 (Clockwise)

Preceding gene: 157963668

Following gene: 157963670

Centisome position: 90.61

GC content: 50.45

Gene sequence:

>1122_bases
ATGTTGAAAATTACCGATATTGAAGTGCTCCACCTACGCGTTCCAGCAATGGATGCCGATTGTGAATGGGGCGAAGACGC
CGTCATCGTTAAAGTGCATACCGATAAAGGTATCGTAGGTGTGGGCGAAGCCGACTCTAGCCCGCTAGTGGTTCAAGCTT
GTATCGAAGCGCCACAAACTAACTTCTACTGCAACGGTCTTAAGCGTCTGCTGATCGGTGAAAATGCGCTGGAAATCGAG
CGCCTGTGGAACAAGATGTACTGGGGTTCAAACTACATGGGTCGTCGCGGTGCTGGCATCCATGCCATTAGTGCTATCGA
CATCGCTCTGTGGGATATCGCAGGTCAGTTCTACGGTGTACCAGTACACACGCTACTTGGCGGTAAGTACCGCGACAAGA
TCCGTTGTTACGGTACCTTTATTCCTGCCGACAAGCCTGAAGACAACGTGGCTATTGTGCAGGGCTTGAAAGACCAAGGT
TTCTCTAGCATTAAGTTTGGTGGCGGCGTGATGGGCGATGACCCAGACACCGACTACGCTATCGTTAAAGCGGTGCGTGA
AGCGGCAGGTCCAGAGATGGAAGTGCAGATCGACTTAGCGTCTAAGTGGCACACCTGTGGTCATTCAGCCATGATGGCGA
AACGTCTAGAAGAGTTTAACCTCAACTGGATTGAAGAGCCGGTACTAGCAGACAGCCTGATCAGCTATGAAAAGCTGTCA
CGTCAGGTATCGCAAAAGATTGCTGGCGGTGAGTCGCTAACCACTCGTTACGAGTTCCAAGAGTTCATCACTAAGTCTAA
CGCCGATATCGTTCAGCCAGACATCACCCGTTGTGGTGGTATCACTGAAATGAAGAAGATCTACGACATCGCGCAGATGA
ATGGTACTCAGCTGATCCCTCATGGTTTCAGCACCGGCATTCTGTTGCATGCTTCGGTACACTTCTTAGCGGCTTGCGAG
CAAGGTACGCTGATGGAGTTCTCTCAGAGCAGCAGCCCACTATTCACTAGCTTAGTGAAAAACCAGCTGCAGTTCGATAA
TGGCTTTGTGGCCGTATCTGACGCACCAGGTTTAGGTATCGAGCTAGACGAAGAGTTAATTGCCAAGTACCGCGTTAACT
AG

Upstream 100 bases:

>100_bases
AACTGCTGCACCATTTGTAATCGTACTGATGCTATGTATGTTCGGCTTAAAGAAATTCCTGTCAAACCACAAGTTTGACA
ACGTTTCAGAGAAATAAATC

Downstream 100 bases:

>100_bases
CACCGTGTAAATTAACATCGCGCTATCTAGCGGCGATTGATTTACCCGCTAAATAACAATGCCAGTAAGTTAAGCTTACT
GGCATTTTTTTATGTCTGTT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLKITDIEVLHLRVPAMDADCEWGEDAVIVKVHTDKGIVGVGEADSSPLVVQACIEAPQTNFYCNGLKRLLIGENALEIE
RLWNKMYWGSNYMGRRGAGIHAISAIDIALWDIAGQFYGVPVHTLLGGKYRDKIRCYGTFIPADKPEDNVAIVQGLKDQG
FSSIKFGGGVMGDDPDTDYAIVKAVREAAGPEMEVQIDLASKWHTCGHSAMMAKRLEEFNLNWIEEPVLADSLISYEKLS
RQVSQKIAGGESLTTRYEFQEFITKSNADIVQPDITRCGGITEMKKIYDIAQMNGTQLIPHGFSTGILLHASVHFLAACE
QGTLMEFSQSSSPLFTSLVKNQLQFDNGFVAVSDAPGLGIELDEELIAKYRVN

Sequences:

>Translated_373_residues
MLKITDIEVLHLRVPAMDADCEWGEDAVIVKVHTDKGIVGVGEADSSPLVVQACIEAPQTNFYCNGLKRLLIGENALEIE
RLWNKMYWGSNYMGRRGAGIHAISAIDIALWDIAGQFYGVPVHTLLGGKYRDKIRCYGTFIPADKPEDNVAIVQGLKDQG
FSSIKFGGGVMGDDPDTDYAIVKAVREAAGPEMEVQIDLASKWHTCGHSAMMAKRLEEFNLNWIEEPVLADSLISYEKLS
RQVSQKIAGGESLTTRYEFQEFITKSNADIVQPDITRCGGITEMKKIYDIAQMNGTQLIPHGFSTGILLHASVHFLAACE
QGTLMEFSQSSSPLFTSLVKNQLQFDNGFVAVSDAPGLGIELDEELIAKYRVN
>Mature_373_residues
MLKITDIEVLHLRVPAMDADCEWGEDAVIVKVHTDKGIVGVGEADSSPLVVQACIEAPQTNFYCNGLKRLLIGENALEIE
RLWNKMYWGSNYMGRRGAGIHAISAIDIALWDIAGQFYGVPVHTLLGGKYRDKIRCYGTFIPADKPEDNVAIVQGLKDQG
FSSIKFGGGVMGDDPDTDYAIVKAVREAAGPEMEVQIDLASKWHTCGHSAMMAKRLEEFNLNWIEEPVLADSLISYEKLS
RQVSQKIAGGESLTTRYEFQEFITKSNADIVQPDITRCGGITEMKKIYDIAQMNGTQLIPHGFSTGILLHASVHFLAACE
QGTLMEFSQSSSPLFTSLVKNQLQFDNGFVAVSDAPGLGIELDEELIAKYRVN

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=362, Percent_Identity=32.0441988950276, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1787864, Length=387, Percent_Identity=28.1653746770026, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI226510960, Length=312, Percent_Identity=30.1282051282051, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 41070; Mature: 41070

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKITDIEVLHLRVPAMDADCEWGEDAVIVKVHTDKGIVGVGEADSSPLVVQACIEAPQT
CEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCEEHEECCCCCC
NFYCNGLKRLLIGENALEIERLWNKMYWGSNYMGRRGAGIHAISAIDIALWDIAGQFYGV
CEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHCCHHCCC
PVHTLLGGKYRDKIRCYGTFIPADKPEDNVAIVQGLKDQGFSSIKFGGGVMGDDPDTDYA
CHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCHHCCCCEEECCCCCCCCCCCCCHH
IVKAVREAAGPEMEVQIDLASKWHTCGHSAMMAKRLEEFNLNWIEEPVLADSLISYEKLS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RQVSQKIAGGESLTTRYEFQEFITKSNADIVQPDITRCGGITEMKKIYDIAQMNGTQLIP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEECC
HGFSTGILLHASVHFLAACEQGTLMEFSQSSSPLFTSLVKNQLQFDNGFVAVSDAPGLGI
CCCCCCEEEEHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
ELDEELIAKYRVN
EECHHHHHHHCCC
>Mature Secondary Structure
MLKITDIEVLHLRVPAMDADCEWGEDAVIVKVHTDKGIVGVGEADSSPLVVQACIEAPQT
CEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCEEHEECCCCCC
NFYCNGLKRLLIGENALEIERLWNKMYWGSNYMGRRGAGIHAISAIDIALWDIAGQFYGV
CEEHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHCCHHCCC
PVHTLLGGKYRDKIRCYGTFIPADKPEDNVAIVQGLKDQGFSSIKFGGGVMGDDPDTDYA
CHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCHHCCCCEEECCCCCCCCCCCCCHH
IVKAVREAAGPEMEVQIDLASKWHTCGHSAMMAKRLEEFNLNWIEEPVLADSLISYEKLS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RQVSQKIAGGESLTTRYEFQEFITKSNADIVQPDITRCGGITEMKKIYDIAQMNGTQLIP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCEECC
HGFSTGILLHASVHFLAACEQGTLMEFSQSSSPLFTSLVKNQLQFDNGFVAVSDAPGLGI
CCCCCCEEEEHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
ELDEELIAKYRVN
EECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA