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The map label for this gene is folE [H]

Identifier: 157963653

GI number: 157963653

Start: 4669611

End: 4670270

Strand: Direct

Name: folE [H]

Synonym: Spea_3842

Alternate gene names: 157963653

Gene position: 4669611-4670270 (Clockwise)

Preceding gene: 157963652

Following gene: 157963656

Centisome position: 90.24

GC content: 48.64

Gene sequence:

>660_bases
ATGACATCAAAAGAAGCAATCCTCAGTGAATCTGCTCAAATCGTACAATCTGCATTAATTGAGCGCGGCCTCGAGACGCC
GATGTTGCCGAGTGAGCTAAGCGCTGAAGTACGCAAAGACAAGATTGAGCATCATATGCGTGAAATCTTGACCTTAATGT
CACTAGACTTAAGCGATGATAGCCTAGCCGATACCCCGCGTCGTATCGCCAAGATGTATGTCGATGAGATATTCTCAGGC
CTAGACTACGCCAACTTCCCCAAGATCACCGTTATCGAAAACAAGATGGGCTTCGATGAGATGGTAAAGGTGAATGACAT
CAGCTTAACCAGTACCTGTGAACATCACTTAGTGACTATCGATGGCCTAGCGACCGTAGCCTACTTACCGCGCAAGAACA
TCATTGGCCTGTCTAAGATTAACCGTATCGTACGTTTCTTTGCTCAGCGCCCTCAGGTGCAAGAACGTCTGACTCAGCAG
GTTCTAGTGGCGCTACAGGCACTACTAGGCACTAAAGACGTGGCAGTTAAGATGGATGCGGTGCATTACTGCGTTAAATC
ACGCGGCGTGATGGACTCGACCAGCTCGACCACCACTACAGCACTAGGCGGCATCTTTAAGTCCAACCCAGCGACAAGAG
CTGAGTTCTTAAGCAATTAG

Upstream 100 bases:

>100_bases
AACTTGTTTTTATGAAATAACTTAGTCTCAATGAGACTGACGGCCCTAGTACACAGGATACGAATCTCACCACTCATTAT
TTATGGTGAAGGAGGAACTT

Downstream 100 bases:

>100_bases
GTCCTAGGTCCTAGGTCCTAGGTCCTAAAAGATTAAAGCACCAAAACAAAAACGCTGCGTCATAAACGCAGCGTTTTTTC
GTTTTCGGCGAAGCAAAAGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTSKEAILSESAQIVQSALIERGLETPMLPSELSAEVRKDKIEHHMREILTLMSLDLSDDSLADTPRRIAKMYVDEIFSG
LDYANFPKITVIENKMGFDEMVKVNDISLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQ
VLVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN

Sequences:

>Translated_219_residues
MTSKEAILSESAQIVQSALIERGLETPMLPSELSAEVRKDKIEHHMREILTLMSLDLSDDSLADTPRRIAKMYVDEIFSG
LDYANFPKITVIENKMGFDEMVKVNDISLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQ
VLVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
>Mature_218_residues
TSKEAILSESAQIVQSALIERGLETPMLPSELSAEVRKDKIEHHMREILTLMSLDLSDDSLADTPRRIAKMYVDEIFSGL
DYANFPKITVIENKMGFDEMVKVNDISLTSTCEHHLVTIDGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQV
LVALQALLGTKDVAVKMDAVHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=120, Percent_Identity=47.5, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503949, Length=120, Percent_Identity=47.5, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI66932970, Length=98, Percent_Identity=44.8979591836735, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI66932972, Length=82, Percent_Identity=50, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1788476, Length=212, Percent_Identity=70.7547169811321, Blast_Score=301, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=39.5480225988701, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321706, Length=183, Percent_Identity=40.9836065573771, Blast_Score=128, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24656782, Length=120, Percent_Identity=48.3333333333333, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24656796, Length=120, Percent_Identity=48.3333333333333, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24656791, Length=120, Percent_Identity=48.3333333333333, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 24315; Mature: 24184

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKEAILSESAQIVQSALIERGLETPMLPSELSAEVRKDKIEHHMREILTLMSLDLSDD
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLADTPRRIAKMYVDEIFSGLDYANFPKITVIENKMGFDEMVKVNDISLTSTCEHHLVTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHEECCCCHHHHHCHHEEEE
DGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLGTKDVAVKMDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TSKEAILSESAQIVQSALIERGLETPMLPSELSAEVRKDKIEHHMREILTLMSLDLSDD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SLADTPRRIAKMYVDEIFSGLDYANFPKITVIENKMGFDEMVKVNDISLTSTCEHHLVTI
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHEECCCCHHHHHCHHEEEE
DGLATVAYLPRKNIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLGTKDVAVKMDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VHYCVKSRGVMDSTSSTTTTALGGIFKSNPATRAEFLSN
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA