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The map label for this gene is mraW [H]

Identifier: 157963630

GI number: 157963630

Start: 4643569

End: 4644516

Strand: Reverse

Name: mraW [H]

Synonym: Spea_3819

Alternate gene names: 157963630

Gene position: 4644516-4643569 (Counterclockwise)

Preceding gene: 157963631

Following gene: 157963629

Centisome position: 89.76

GC content: 49.05

Gene sequence:

>948_bases
ATGACACAAGAATTTGAACACCTATCAGTACTGCTAGCGGAAACCGTTGCAGGCTTAAACATTAGACCCGATGGCATCTA
TATCGATGGTACTTTCGGTCGTGGTGGTCATTCTAGAAAAGTTCTTGAGGAACTTGGACCGAATGGAAGACTGATTGCTA
TCGACCGTGATCCGCAGGCTATCGCCGCGGCTGAGCAGTTTAAAGACGACAGTCGTTTTCAGATCGTTCATGGTGGTTTC
GGCCAGTTAGCTGACTATGTCGAAGACTTAGGCTTAAAGGGCCAAATCGATGGCGTATTACTGGACTTTGGCGTGTCATC
GCCGCAGTTAGACGATGCCGAGCGTGGCTTTAGCTTCTTGCGAGATGGTCCATTAGATATGCGTATGGATAACTCTCAAG
GCGAGACTGCTGCTCAGTGGCTTGCCCGCGCTGAAATTGAAGATATGGCATGGGTGTTTAAGACTTATGGCGAGGAGAAA
AACTCCCGTCATATCGCTCGCTGTATCGCCGCAGACCGTGAAAAGGCTCCGTTTTTACGTACCAAAGATCTGGCCGATTT
GATTGCCCGCATCACTAAGAACAAGGAGCGTAATAAGCATCCTGCGACACGCGTATTCCAAGCTATTCGTATCTACATCA
ACAGTGAACTAGAGCAGATCGATCAAGCCCTAGAAGGCGCATTAGCCGTGTTGGCTCCAGAAGGCCGCTTATCTGTGATT
AGCTTCCATTCACTGGAAGACCGTATGGTGAAGCGCTTTATTCGCCGTAATAGCCAAGGTGAGAGTGTACCTCATGGTTT
GCCTATTACTGAGGCTGAGATCAATAAGTCGCGCAAGCTAAAGGCAATGGGCAAAGCCATTAAGCCTTCGGTAGAAGAGG
TTGAGCGTAACGCGAGAGCCCGAAGCTCTGTGCTACGTATCGCCGAGCGTTTACCTTACGAAGCATAA

Upstream 100 bases:

>100_bases
ACAGAGTCGAATAGCGATTCTTAATGAAGACTTGGCTGCCAATGAACGTTTGGCAGATTTTTCACTTTAGTCAGTGATAT
TTAAATAGTAGAAGAAACCC

Downstream 100 bases:

>100_bases
ACGATTAGAGACTTAAGTGAGTAAAGCTCAATTGAATTTAACCCGCATAGTGCTGCTTGATTTATGGCATCACAAATGGG
TCATGATACTCGCGCTGCTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTQEFEHLSVLLAETVAGLNIRPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGF
GQLADYVEDLGLKGQIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEEK
NSRHIARCIAADREKAPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALAVLAPEGRLSVI
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEAEINKSRKLKAMGKAIKPSVEEVERNARARSSVLRIAERLPYEA

Sequences:

>Translated_315_residues
MTQEFEHLSVLLAETVAGLNIRPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGF
GQLADYVEDLGLKGQIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEEK
NSRHIARCIAADREKAPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALAVLAPEGRLSVI
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEAEINKSRKLKAMGKAIKPSVEEVERNARARSSVLRIAERLPYEA
>Mature_314_residues
TQEFEHLSVLLAETVAGLNIRPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQAIAAAEQFKDDSRFQIVHGGFG
QLADYVEDLGLKGQIDGVLLDFGVSSPQLDDAERGFSFLRDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEEKN
SRHIARCIAADREKAPFLRTKDLADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALAVLAPEGRLSVIS
FHSLEDRMVKRFIRRNSQGESVPHGLPITEAEINKSRKLKAMGKAIKPSVEEVERNARARSSVLRIAERLPYEA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=37.1681415929204, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=39.1752577319588, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=63.1410256410256, Blast_Score=399, Evalue=1e-112,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=41.6918429003021, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=41.6918429003021, Blast_Score=198, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35217; Mature: 35086

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEFEHLSVLLAETVAGLNIRPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQA
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IAAAEQFKDDSRFQIVHGGFGQLADYVEDLGLKGQIDGVLLDFGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEEKNSRHIARCIAADREKAPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
TKDLADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALAVLAPEGRLSVI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEE
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEAEINKSRKLKAMGKAIKPSVEEVERNARA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHCCCCHHHHHHHHHH
RSSVLRIAERLPYEA
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQEFEHLSVLLAETVAGLNIRPDGIYIDGTFGRGGHSRKVLEELGPNGRLIAIDRDPQA
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEECCCCHH
IAAAEQFKDDSRFQIVHGGFGQLADYVEDLGLKGQIDGVLLDFGVSSPQLDDAERGFSFL
HHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
RDGPLDMRMDNSQGETAAQWLARAEIEDMAWVFKTYGEEKNSRHIARCIAADREKAPFLR
HCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
TKDLADLIARITKNKERNKHPATRVFQAIRIYINSELEQIDQALEGALAVLAPEGRLSVI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEE
SFHSLEDRMVKRFIRRNSQGESVPHGLPITEAEINKSRKLKAMGKAIKPSVEEVERNARA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHCCCCHHHHHHHHHH
RSSVLRIAERLPYEA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA