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The map label for this gene is murF [H]

Identifier: 157963626

GI number: 157963626

Start: 4638663

End: 4640042

Strand: Reverse

Name: murF [H]

Synonym: Spea_3815

Alternate gene names: 157963626

Gene position: 4640042-4638663 (Counterclockwise)

Preceding gene: 157963627

Following gene: 157963625

Centisome position: 89.67

GC content: 47.75

Gene sequence:

>1380_bases
ATGATTAGGGTTTCTTTAGCCGAGTTAGCGACGCGCCTGAATGGCCAGTTGATTGGAGATGATCTGCCAATCGATAGCGT
CTCTAGCGACAGTCGAGCAGTAGATAGCAAGACGCTTTTTGTCGCATTGAAAGGTGATAATTTTGATGGTCATAACTTCG
CTCAGGCAGCGGTCGATGCTGGTGCGAACGCATTGTTGGTAGAAAGAGAGTTACCGATAGACGCCGCACAAATTGTCGTG
GCAAATAGCCAGCGTGCGATGGGCGAAATTGGCGCCTATGTGCGTGAACAGCTTGCGCCAACTTGTGTTGCCTTAACCGG
TTCAAATGGTAAGACTAGCGTAAAAGAGATGATAGCGACTATCTTGTCGCAACAGCATCGAGTGCTCTATACCGCGGGTA
ACTTCAATAACGAAATTGGTGTGCCGTTAACCTTGCTGAGATTGAAGCAAGGTGATGAGTTTGGCGTATTTGAGCTTGGC
GCTAACCACGCAGGTGAGATTGACTATACCTCTGGTTTGGTCAAACCGAGTGTGGCCATGGTGAATAACGTCGCTTCGGC
TCACCTTGAAGGGTTCGGCTCTTTAGAGGGGGTGGCTAAGGCTAAGTCTGAGATCTTTAATCACGTTACCGTAGGCGGTA
CAGCAGTGATTAATGCCGATGATGCATTTGCTGATGTCATGCTTCAGGCGTCTAGCCACGTTAAGCAGTTATGTTTTGGT
GTTAAAAATGAAGCGTCGAGAGATCTCGATGTTTTTGCTACAGATTTGATTGCAGATTCTTACGGACGTTATGCCTTTAG
CCTTTGCTACGGTGCAGAAGTTCATAGAGTTGAGTTGCCGTTGGCTGGGCGTCACCAGGTGTCTAATGCGCTAGCGGCGA
CCAGTATCTGTTTAGCATTAGGTTTGTCTTTGGCTCATATTTGCCAAGGACTTAGCCAACTAAAACCTGTGAAGGGCCGC
ATGCTGCCAAGTGTACTTGGGCGGGTGACGGTAATCGATGATAGCTATAACGCAAATCCGGCTTCAGTGGGCGCAGCTAT
CGACTGGCTTCAAGAAATTAAAGGAAATCGCTGTTTAGTACTGGGCGATTTGGGCGAATTAGGCGACAATGCCGCCCTTT
TGCATCGCGAGCTTGGGGCGTTAGCCAAGGCAAAAGGCGTAAATTCGCTGTTTTGTGTCGGTACGCTAAGCGAGGGTGCG
AGTCATGCTTTTGGTTCCAAACACTATCAAGATATTGATGATTTGGTTGCAGGATTAATTCAATACATTAATGAATTACC
TGCCGAAATTACCGTGTTAGTGAAAGGTTCTCGCAGTGCGAGAATGGAACGTGTAGTAGAAGCTTTAACAACGGCCTTTG
GTCGCGGGGAGTTTGTTTAA

Upstream 100 bases:

>100_bases
TGCTTGCAGGAAAAGGCCATGAAACTTACCAAGAAGTAAATGGTAAAAAACTAAATTACGATGAAAGAGCATTCGCTAAA
GCGATTGCCGAAGGTAACGC

Downstream 100 bases:

>100_bases
TGCTGGTTTACCTGGCTGAGTATTTAACCCAATTTTACACTGGGTTTAATGTATTTTCTTACGTTACCTTCCGAGCCATT
CTTGGTTTGATGACGGCAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIRVSLAELATRLNGQLIGDDLPIDSVSSDSRAVDSKTLFVALKGDNFDGHNFAQAAVDAGANALLVERELPIDAAQIVV
ANSQRAMGEIGAYVREQLAPTCVALTGSNGKTSVKEMIATILSQQHRVLYTAGNFNNEIGVPLTLLRLKQGDEFGVFELG
ANHAGEIDYTSGLVKPSVAMVNNVASAHLEGFGSLEGVAKAKSEIFNHVTVGGTAVINADDAFADVMLQASSHVKQLCFG
VKNEASRDLDVFATDLIADSYGRYAFSLCYGAEVHRVELPLAGRHQVSNALAATSICLALGLSLAHICQGLSQLKPVKGR
MLPSVLGRVTVIDDSYNANPASVGAAIDWLQEIKGNRCLVLGDLGELGDNAALLHRELGALAKAKGVNSLFCVGTLSEGA
SHAFGSKHYQDIDDLVAGLIQYINELPAEITVLVKGSRSARMERVVEALTTAFGRGEFV

Sequences:

>Translated_459_residues
MIRVSLAELATRLNGQLIGDDLPIDSVSSDSRAVDSKTLFVALKGDNFDGHNFAQAAVDAGANALLVERELPIDAAQIVV
ANSQRAMGEIGAYVREQLAPTCVALTGSNGKTSVKEMIATILSQQHRVLYTAGNFNNEIGVPLTLLRLKQGDEFGVFELG
ANHAGEIDYTSGLVKPSVAMVNNVASAHLEGFGSLEGVAKAKSEIFNHVTVGGTAVINADDAFADVMLQASSHVKQLCFG
VKNEASRDLDVFATDLIADSYGRYAFSLCYGAEVHRVELPLAGRHQVSNALAATSICLALGLSLAHICQGLSQLKPVKGR
MLPSVLGRVTVIDDSYNANPASVGAAIDWLQEIKGNRCLVLGDLGELGDNAALLHRELGALAKAKGVNSLFCVGTLSEGA
SHAFGSKHYQDIDDLVAGLIQYINELPAEITVLVKGSRSARMERVVEALTTAFGRGEFV
>Mature_459_residues
MIRVSLAELATRLNGQLIGDDLPIDSVSSDSRAVDSKTLFVALKGDNFDGHNFAQAAVDAGANALLVERELPIDAAQIVV
ANSQRAMGEIGAYVREQLAPTCVALTGSNGKTSVKEMIATILSQQHRVLYTAGNFNNEIGVPLTLLRLKQGDEFGVFELG
ANHAGEIDYTSGLVKPSVAMVNNVASAHLEGFGSLEGVAKAKSEIFNHVTVGGTAVINADDAFADVMLQASSHVKQLCFG
VKNEASRDLDVFATDLIADSYGRYAFSLCYGAEVHRVELPLAGRHQVSNALAATSICLALGLSLAHICQGLSQLKPVKGR
MLPSVLGRVTVIDDSYNANPASVGAAIDWLQEIKGNRCLVLGDLGELGDNAALLHRELGALAKAKGVNSLFCVGTLSEGA
SHAFGSKHYQDIDDLVAGLIQYINELPAEITVLVKGSRSARMERVVEALTTAFGRGEFV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=452, Percent_Identity=47.3451327433628, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48501; Mature: 48501

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVSLAELATRLNGQLIGDDLPIDSVSSDSRAVDSKTLFVALKGDNFDGHNFAQAAVDA
CEEEEHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHC
GANALLVERELPIDAAQIVVANSQRAMGEIGAYVREQLAPTCVALTGSNGKTSVKEMIAT
CCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
ILSQQHRVLYTAGNFNNEIGVPLTLLRLKQGDEFGVFELGANHAGEIDYTSGLVKPSVAM
HHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCHHHH
VNNVASAHLEGFGSLEGVAKAKSEIFNHVTVGGTAVINADDAFADVMLQASSHVKQLCFG
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHC
VKNEASRDLDVFATDLIADSYGRYAFSLCYGAEVHRVELPLAGRHQVSNALAATSICLAL
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GLSLAHICQGLSQLKPVKGRMLPSVLGRVTVIDDSYNANPASVGAAIDWLQEIKGNRCLV
HHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
LGDLGELGDNAALLHRELGALAKAKGVNSLFCVGTLSEGASHAFGSKHYQDIDDLVAGLI
EECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHH
QYINELPAEITVLVKGSRSARMERVVEALTTAFGRGEFV
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIRVSLAELATRLNGQLIGDDLPIDSVSSDSRAVDSKTLFVALKGDNFDGHNFAQAAVDA
CEEEEHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHC
GANALLVERELPIDAAQIVVANSQRAMGEIGAYVREQLAPTCVALTGSNGKTSVKEMIAT
CCCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
ILSQQHRVLYTAGNFNNEIGVPLTLLRLKQGDEFGVFELGANHAGEIDYTSGLVKPSVAM
HHCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCHHHH
VNNVASAHLEGFGSLEGVAKAKSEIFNHVTVGGTAVINADDAFADVMLQASSHVKQLCFG
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHC
VKNEASRDLDVFATDLIADSYGRYAFSLCYGAEVHRVELPLAGRHQVSNALAATSICLAL
CCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHH
GLSLAHICQGLSQLKPVKGRMLPSVLGRVTVIDDSYNANPASVGAAIDWLQEIKGNRCLV
HHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
LGDLGELGDNAALLHRELGALAKAKGVNSLFCVGTLSEGASHAFGSKHYQDIDDLVAGLI
EECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHHHH
QYINELPAEITVLVKGSRSARMERVVEALTTAFGRGEFV
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2668880; 8973200; 1630901; 9278503; 9166795; 2186811; 11090285 [H]