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The map label for this gene is hslU [H]

Identifier: 157963594

GI number: 157963594

Start: 4597819

End: 4599144

Strand: Direct

Name: hslU [H]

Synonym: Spea_3782

Alternate gene names: 157963594

Gene position: 4597819-4599144 (Clockwise)

Preceding gene: 157963593

Following gene: 157963595

Centisome position: 88.85

GC content: 46.76

Gene sequence:

>1326_bases
ATGTCTGAGATGACACCACGTGAAATTGTTCACGAGCTAGATAGCCACATTATCGGTCAACAAAAAGCTAAGCGCTCTGT
CGCTATCGCTCTGCGTAACCGCTGGCGCCGTATGCAGCTTGCTGCTGACTTACGTCAAGAAGTGACGCCAAAGAACATCC
TAATGATTGGCCCAACCGGTGTAGGTAAAACTGAGATTGCTCGTCGTCTAGCTCGCCTAGCTAAAGCGCCATTTATTAAA
GTTGAAGCCACTAAGTTCACCGAAGTAGGCTATGTTGGTAAAGAAGTCGAGCAGATCATTCGCGATCTAACCGATTCGGC
TATCAAGCTAACCCGCGAAGAGCAAATCAAAAAATGTAAGTTCCGCGCTGAAGAAGCCGCTGAAGAGCGCATTTTAGATG
CCTTGTTGCCTAAGCCTAAGGAAGATTGGGACAGCGAAAAATCAGATGGCAGCGCCACACGTCAAATTTTCCGTAAGAAG
CTTCGTGAAGGTCAGCTAGACGATAAAGAAATTGAGATCGATGTCTCTGCGCCACAGGCCGGTATTGAAATCATGTCTCC
TCCTGGTATGGAAGAGATGACTAACCAGTTGCAGAGCATGTTCCAAAATATGGGACCGGGTGCTAGTAAGCGCCGAAAAA
TGCCAATCAAAGAAGCTTATAAGCTATTGATTGAAGAAGAAGCATCTAAGCTGATTAATCAAGAAGACTTAAAAGAGCAG
GCGATCGAGCTTGTTGAGCAACACGGCATCGTATTCCTCGATGAGATCGATAAGATCTGTAAGCGTGGCGAATCGTCAGG
CCCAGATGTGTCTCGTGAAGGTGTTCAGCGTGATCTACTGCCTCTTGTTGAAGGTTGTACGGTTAACACTAAACACGGCA
TGGTAAAAACCGACCACATCTTGTTTATCGCCTCGGGGGCTTTCCAGATGTCTAAGCCATCGGATCTGATCCCTGAGCTT
CAAGGCCGTCTACCAATTCGTGTTGAATTAGATGCGTTAACCGCTGGTGACTTTAAGCGTATTCTGACTGAGCCTCATGC
CTCGTTGACTGAGCAATATATTGCTTTGATGGGTACTGAAGGCGTCACAATCGAGTTTACTGAAGATGGTATCGACAGCA
TTGCTGAAGCCGCTTGGCAAGTGAACGAGCGCACTGAAAACATCGGTGCCCGTCGTCTACACACTGTGATGGAGCGCTTG
ATGGAAGAGCTTTCTTATGAAGCCTCTGACAAGTCAGGTTCTGTTACCGTTATCGATGCGGCTTATGTTAAGGCAAGCTT
AGATAACTTGGTGCAAGACGAAGACTTAAGTCGCTATATTCTTTAA

Upstream 100 bases:

>100_bases
ATTTGCGTATTTACCAACCAATTTAAGACTATTGAAGAATTAAAGTACTAAGCGACTCAGTCGTTTATACGAAAAATGCT
GCAAAGCATGAGGAAAGATT

Downstream 100 bases:

>100_bases
GCTAAGGCTAAAGTATCAGCTTAAAATCGACACCAAAGTAATGGCAAAATAAGTGATAGCGGTTAAGGACAAGATTGTGC
TTAACCGCTATTGTGTTTCT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSEMTPREIVHELDSHIIGQQKAKRSVAIALRNRWRRMQLAADLRQEVTPKNILMIGPTGVGKTEIARRLARLAKAPFIK
VEATKFTEVGYVGKEVEQIIRDLTDSAIKLTREEQIKKCKFRAEEAAEERILDALLPKPKEDWDSEKSDGSATRQIFRKK
LREGQLDDKEIEIDVSAPQAGIEIMSPPGMEEMTNQLQSMFQNMGPGASKRRKMPIKEAYKLLIEEEASKLINQEDLKEQ
AIELVEQHGIVFLDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPEL
QGRLPIRVELDALTAGDFKRILTEPHASLTEQYIALMGTEGVTIEFTEDGIDSIAEAAWQVNERTENIGARRLHTVMERL
MEELSYEASDKSGSVTVIDAAYVKASLDNLVQDEDLSRYIL

Sequences:

>Translated_441_residues
MSEMTPREIVHELDSHIIGQQKAKRSVAIALRNRWRRMQLAADLRQEVTPKNILMIGPTGVGKTEIARRLARLAKAPFIK
VEATKFTEVGYVGKEVEQIIRDLTDSAIKLTREEQIKKCKFRAEEAAEERILDALLPKPKEDWDSEKSDGSATRQIFRKK
LREGQLDDKEIEIDVSAPQAGIEIMSPPGMEEMTNQLQSMFQNMGPGASKRRKMPIKEAYKLLIEEEASKLINQEDLKEQ
AIELVEQHGIVFLDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPEL
QGRLPIRVELDALTAGDFKRILTEPHASLTEQYIALMGTEGVTIEFTEDGIDSIAEAAWQVNERTENIGARRLHTVMERL
MEELSYEASDKSGSVTVIDAAYVKASLDNLVQDEDLSRYIL
>Mature_440_residues
SEMTPREIVHELDSHIIGQQKAKRSVAIALRNRWRRMQLAADLRQEVTPKNILMIGPTGVGKTEIARRLARLAKAPFIKV
EATKFTEVGYVGKEVEQIIRDLTDSAIKLTREEQIKKCKFRAEEAAEERILDALLPKPKEDWDSEKSDGSATRQIFRKKL
REGQLDDKEIEIDVSAPQAGIEIMSPPGMEEMTNQLQSMFQNMGPGASKRRKMPIKEAYKLLIEEEASKLINQEDLKEQA
IELVEQHGIVFLDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHILFIASGAFQMSKPSDLIPELQ
GRLPIRVELDALTAGDFKRILTEPHASLTEQYIALMGTEGVTIEFTEDGIDSIAEAAWQVNERTENIGARRLHTVMERLM
EELSYEASDKSGSVTVIDAAYVKASLDNLVQDEDLSRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=77.2009029345372, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=181, Percent_Identity=35.9116022099448, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648291, Length=275, Percent_Identity=31.6363636363636, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648289, Length=275, Percent_Identity=31.6363636363636, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 49607; Mature: 49476

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELDSHIIGQQKAKRSVAIALRNRWRRMQLAADLRQEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLAKAPFIKVEATKFTEVGYVGKEVEQIIRDLTDSAIKLTREEQIKKCK
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEAAEERILDALLPKPKEDWDSEKSDGSATRQIFRKKLREGQLDDKEIEIDVSAPQA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GIEIMSPPGMEEMTNQLQSMFQNMGPGASKRRKMPIKEAYKLLIEEEASKLINQEDLKEQ
CCEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AIELVEQHGIVFLDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHI
HHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCE
LFIASGAFQMSKPSDLIPELQGRLPIRVELDALTAGDFKRILTEPHASLTEQYIALMGTE
EEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCC
GVTIEFTEDGIDSIAEAAWQVNERTENIGARRLHTVMERLMEELSYEASDKSGSVTVIDA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
AYVKASLDNLVQDEDLSRYIL
HHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELDSHIIGQQKAKRSVAIALRNRWRRMQLAADLRQEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLAKAPFIKVEATKFTEVGYVGKEVEQIIRDLTDSAIKLTREEQIKKCK
CCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEAAEERILDALLPKPKEDWDSEKSDGSATRQIFRKKLREGQLDDKEIEIDVSAPQA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GIEIMSPPGMEEMTNQLQSMFQNMGPGASKRRKMPIKEAYKLLIEEEASKLINQEDLKEQ
CCEECCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AIELVEQHGIVFLDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVNTKHGMVKTDHI
HHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCE
LFIASGAFQMSKPSDLIPELQGRLPIRVELDALTAGDFKRILTEPHASLTEQYIALMGTE
EEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHCCC
GVTIEFTEDGIDSIAEAAWQVNERTENIGARRLHTVMERLMEELSYEASDKSGSVTVIDA
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEH
AYVKASLDNLVQDEDLSRYIL
HHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA